FastQCFastQC Report
Thu 26 May 2016
SRR1926542_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926542_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643531
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC24240.37667183088304995No Hit
GTACATGGGAAGCAGTGGTATCAAC23770.3693683754162581No Hit
GTCCTACAGTGGACATTTCTAAATT11210.17419518251645996No Hit
GAGTACATGGGAAGCAGTGGTATCA10960.17031036577880473No Hit
CATGTACTCTGCGTTGATACCACTG8240.12804355967311598No Hit
CTGTAGGACGTGGAATATGGCAAGA7990.12415874293546075No Hit
GCGTTGATACCACTGCTTCCCATGT7820.12151706755385522No Hit
GTCCTAAAGTGTGTATTTCTCATTT7310.11359204140903859No Hit
ACGCAGAGTACATGGGAAGCAGTGG6770.10520083725570331No Hit
GTATCAACGCAGAGTACATGGGAAG6650.1033361252216288No Hit
GTCCTACAGTGTGCATTTCTCATTT6480.10069444984002324No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGCGT250.003588203820.7812236
CGCAGAC304.8875477E-420.331477
GCGTCTC304.8875477E-420.331477
TAGGACC2350.018.6677044
GTCCTAC6550.016.0308841
TGTAGGA5950.015.7854662
CTCCGTT350.00270779715.75180511
AGGACCG400.002896976415.5925195
GATATAC2100.015.1902061
TCCTACA7200.014.7464482
CTGTAGG5800.014.208081
GTAGGAC7050.013.9419343
TAGGGTC450.00633656413.8600185
TATACAC3250.013.7774913
CTGTACT1107.179551E-913.6179054
TTTAGGA4550.013.4625222
TTAGGAC4250.013.3623873
GTGTAGG1008.961808E-613.29142951
TAGGACT1055.738002E-813.247354
GTACTGA550.001562527313.2244156
Produced by FastQC (version 0.11.5)