FastQCFastQC Report
Thu 26 May 2016
SRR1926542_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926542_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643531
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC67201.0442387390817225No Hit
GTACATGGGAAGCAGTGGTATCAAC60930.9468075353013297No Hit
GAGTACATGGGAAGCAGTGGTATCA26110.40573026008071095No Hit
GTCCTACAGTGGACATTTCTAAATT25130.3905017784691025No Hit
CTGTAGGACGTGGAATATGGCAAGA24860.38630617639243486No Hit
CATGTACTCTGCGTTGATACCACTG23880.3710776947808264No Hit
GCGTTGATACCACTGCTTCCCATGT19960.31016376833439263No Hit
GTCCTAAAGTGTGTATTTCTCATTT17440.271004815618828No Hit
CTTTAGGACGTGAAATATGGCGAGG17140.26634303553364175No Hit
GTATCAACGCAGAGTACATGGGAAG16760.2604381140924058No Hit
ACGCAGAGTACATGGGAAGCAGTGG16700.2595057580753686No Hit
GTCCTACAGTGTGCATTTCTCATTT15750.24474345447227874No Hit
ACTCTGCGTTGATACCACTGCTTCC13800.214441883918568No Hit
CTGTAGGACCTGGAATATGGCGAGA13020.20232125569708376No Hit
GTATCAACGCAGAGTACTTTTTTTT11750.18258638666979524No Hit
GCTTCCCATGTACTCTGCGTTGATA11050.17170889980436063No Hit
TATCAACGCAGAGTACATGGGAAGC10700.16627015637164333No Hit
CTGAAGGACCTGGAATATGGCGAGA10420.16191916162546946No Hit
GGTATCAACGCAGAGTACATGGGAA9930.15430492081966526No Hit
GATATACACTGTTCTACAAATCCCG9080.1410965439116375No Hit
CAGTGGTATCAACGCAGAGTACATG8300.12897591569015324No Hit
GGTATCAACGCAGAGTACTTTTTTT7090.11017340267990199No Hit
GCAGTGGTATCAACGCAGAGTACAT6690.10395769589965362No Hit
ATACCACTGCTTCCCATGTACTCTG6640.1031807325521226No Hit
CATGGGAAGCAGTGGTATCAACGCA6530.10147141318755429No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCTA400.005121347514.3134695
TGCGGCC400.005354530614.21774912
CCAACGA801.3330828E-714.206719
TAGGACC5450.014.0070644
TCCAACG1152.7284841E-1114.00189518
TATGTCG552.0071972E-413.777266516
ATAGGAC1204.7293724E-1113.5193353
CTATACA655.2203963E-513.2124334
GAGTAAG801.9020918E-613.1175991
AAGACTC603.9357998E-412.72308355
GACAGCC604.0838053E-412.6675497
ATGTCGA604.189731E-412.6291617
TGTCGAG604.189731E-412.6291618
TGTAGGA13000.012.554762
GGCGAGG4800.012.43086319
GTATAAA853.752244E-612.3459761
CTGTAGG13450.012.3418041
GTCCTAA5350.012.3040181
GTAGGAC12850.012.2537343
TGGCGAG12400.012.14538318
Produced by FastQC (version 0.11.5)