##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926542_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 643531 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.50211100941524 31.0 28.0 31.0 23.0 34.0 2 28.942082044221646 31.0 28.0 33.0 23.0 34.0 3 29.320418130595108 31.0 30.0 33.0 25.0 34.0 4 33.057246659446086 35.0 33.0 37.0 28.0 37.0 5 32.834269056191545 35.0 33.0 37.0 26.0 37.0 6 32.98286018855347 35.0 33.0 37.0 27.0 37.0 7 32.802399573602514 35.0 33.0 37.0 26.0 37.0 8 32.79735863540373 35.0 33.0 37.0 26.0 37.0 9 34.00808352666771 38.0 34.0 39.0 25.0 39.0 10 33.889795518786194 37.0 34.0 39.0 25.0 39.0 11 34.15670884541693 38.0 34.0 39.0 27.0 39.0 12 33.97811449642675 37.0 34.0 39.0 25.0 39.0 13 34.12393186963798 38.0 34.0 39.0 26.0 39.0 14 34.77681261664162 39.0 34.0 40.0 24.0 41.0 15 34.91739636474389 39.0 34.0 40.0 25.0 41.0 16 34.86645087804628 39.0 34.0 40.0 25.0 41.0 17 34.820296147349545 39.0 34.0 40.0 25.0 41.0 18 34.792745027046095 39.0 34.0 40.0 25.0 41.0 19 34.784187552736384 39.0 34.0 40.0 24.0 41.0 20 34.80705513798092 39.0 34.0 41.0 24.0 41.0 21 34.71802290798734 39.0 34.0 40.0 24.0 41.0 22 34.67160867153253 39.0 34.0 40.0 24.0 41.0 23 34.33655099754324 39.0 34.0 40.0 18.0 41.0 24 34.43873410915713 39.0 34.0 41.0 19.0 41.0 25 34.23970873198028 39.0 34.0 40.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7810894911930717 1101 2 0.8061911537947637 1101 3 0.7728783422337706 1101 4 0.6427510479777112 1101 5 0.8602894626578106 1101 6 0.46801936273003975 1101 7 0.7239545136791747 1101 8 0.5779335675896746 1101 9 1.2625408326470762 1101 10 1.0049959051060284 1101 11 0.9148935291571547 1101 12 1.1128417455362296 1101 13 0.9102952007444358 1101 14 1.1980474196738413 1101 15 1.0046683151852562 1101 16 1.2505855408175819 1101 17 1.1318093021662747 1101 18 1.0733663197894714 1101 19 1.1892138832003667 1101 20 1.0768229078891807 1101 21 0.8952250114352296 1101 22 0.9467472357520279 1101 23 1.316472481698682 1101 24 1.2235315240567601 1101 25 1.282979271609264 1102 1 0.43115215973346466 1102 2 0.5824682626860991 1102 3 0.46018891232758463 1102 4 0.26038939352824286 1102 5 0.1769554963089206 1102 6 -0.18025440383913605 1102 7 0.0996657066602964 1102 8 0.28389277606702024 1102 9 0.6624041012862207 1102 10 0.6570829574057768 1102 11 0.2644700417478347 1102 12 0.46076228942320085 1102 13 0.25313389571955724 1102 14 0.5094492959319581 1102 15 0.39312211137956865 1102 16 0.3576841773020689 1102 17 0.5351934033474848 1102 18 0.43394970692912693 1102 19 0.3979874788553488 1102 20 0.27408068448532674 1102 21 0.552044488057966 1102 22 0.5453799221434608 1102 23 0.8369480029869933 1102 24 0.49240614899503754 1102 25 0.5122891580462721 1103 1 -0.10069358250536453 1103 2 -0.2115681803933569 1103 3 -0.21201651139049815 1103 4 -0.2852895210948958 1103 5 -0.20083670531534636 1103 6 -0.4904733472747935 1103 7 -0.21322868439832376 1103 8 0.0037575788912747043 1103 9 0.23585384561474143 1103 10 0.23940283962275544 1103 11 0.0413440424127387 1103 12 0.049515910776122496 1103 13 -0.09340622807926025 1103 14 0.34694988959636675 1103 15 0.21238058494962786 1103 16 0.20619526695330848 1103 17 0.13126571958856204 1103 18 0.01064165050726018 1103 19 0.18698964770933912 1103 20 0.2215548402945302 1103 21 0.17022328394822495 1103 22 -0.05464857374838061 1103 23 0.35734789533584177 1103 24 0.16326909958632996 1103 25 0.5146084070212069 1104 1 -0.1377130728432583 1104 2 0.12633330503157225 1104 3 0.030864385432689545 1104 4 -0.11627455917265905 1104 5 0.07293324686249036 1104 6 -0.31716915741358775 1104 7 -0.0680094392470707 1104 8 -0.05357440904320043 1104 9 0.2248965871520383 1104 10 0.051887361639558094 1104 11 -0.013689128539567719 1104 12 -0.11329016874495323 1104 13 -0.29872542447006367 1104 14 0.08950469127052685 1104 15 0.13597664535106446 1104 16 0.07694699318104625 1104 17 0.003487181263921002 1104 18 0.06181782341152342 1104 19 0.20706987942147492 1104 20 -0.07652919688646875 1104 21 -0.03337314561752436 1104 22 -0.13751981077187025 1104 23 0.11964837355746027 1104 24 -0.16242458324306597 1104 25 -0.058545348379738016 1105 1 0.6627504694914101 1105 2 0.5671842254757777 1105 3 0.7919465609853091 1105 4 0.44332607193704376 1105 5 0.7454399802930212 1105 6 -0.012817338799187894 1105 7 0.04710964514298155 1105 8 0.4622226856968652 1105 9 0.9064206912775461 1105 10 0.9136951312101473 1105 11 0.6359296329342072 1105 12 0.5772171551176868 1105 13 0.37767755379702805 1105 14 1.0561559014666386 1105 15 0.9070384897001063 1105 16 0.9345519182045194 1105 17 0.7039028363665665 1105 18 0.5571311736280791 1105 19 0.7543192474390281 1105 20 0.6085299108324875 1105 21 0.6936764620638556 1105 22 0.6801618856048606 1105 23 1.1006710657305874 1105 24 0.6225860301157198 1105 25 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0.621831819494254 1107 13 0.46050170213644037 1107 14 0.8426362741151792 1107 15 0.7050011446772615 1107 16 0.5281704858915219 1107 17 0.5673179796379486 1107 18 0.9066404425722965 1107 19 1.0951551654581664 1107 20 0.6443880303005542 1107 21 0.9474612210773614 1107 22 0.8961924586374579 1107 23 1.324104390668893 1107 24 1.2105640457274518 1107 25 1.1451841842461192 1108 1 0.8595534003398129 1108 2 0.9018849653728154 1108 3 0.697438952139219 1108 4 0.6511030455063107 1108 5 -0.7269468021494205 1108 6 0.3079287448440411 1108 7 0.6683024804771307 1108 8 0.8756574551852481 1108 9 1.102112604292948 1108 10 1.0713757132050006 1108 11 1.0039609078214937 1108 12 0.8034942016707731 1108 13 0.7587022162752532 1108 14 1.0791401301563113 1108 15 1.0717535011468442 1108 16 1.1314266984022296 1108 17 1.179143172440007 1108 18 1.1577115651087126 1108 19 1.2725330574890137 1108 20 1.2733511836853069 1108 21 1.4153275449848124 1108 22 1.0512910019108048 1108 23 1.3892286408831183 1108 24 1.2311295984266835 1108 25 1.3936846126951323 1109 1 0.49988102676360313 1109 2 -0.7632339586013757 1109 3 0.4395518088993917 1109 4 0.5097430684404785 1109 5 -1.0315591693654724 1109 6 -0.03169346808336826 1109 7 0.4338084967785605 1109 8 0.45887133975309524 1109 9 0.989963920735832 1109 10 -1.2185103835853113 1109 11 -0.12983032550071272 1109 12 0.6277455124741138 1109 13 0.7309258744935647 1109 14 1.015826801553935 1109 15 1.0062783457067965 1109 16 1.0833753973114497 1109 17 0.9714151114576879 1109 18 1.1873544407143797 1109 19 1.1935206363839441 1109 20 1.3973241201197624 1109 21 1.2388558970304828 1109 22 1.4096435645372054 1109 23 1.375724272583021 1109 24 1.1707190706432868 1109 25 1.4554141681577377 1110 1 0.7822492840216348 1110 2 -0.03650544103513198 1110 3 0.5757411026325059 1110 4 0.8369972208908862 1110 5 0.9155938317718295 1110 6 0.5203844679605893 1110 7 0.6770180957253373 1110 8 0.8577542434214536 1110 9 1.273881709224007 1110 10 0.8944766759551399 1110 11 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17 0.5676119685894321 2202 18 0.6194907224315429 2202 19 0.49123576856852225 2202 20 0.3308590689703621 2202 21 0.46104624541690953 2202 22 0.5154106759249188 2202 23 0.733343848088353 2202 24 0.5878589827363285 2202 25 0.6277727455690183 2203 1 0.23930878020432544 2203 2 0.23032266241894206 2203 3 0.16059799564700228 2203 4 -0.16499046955407692 2203 5 0.20981574008918358 2203 6 0.13816269417388582 2203 7 0.5009931402886139 2203 8 0.42812386639928945 2203 9 0.6177639276345204 2203 10 0.7942177044420049 2203 11 0.5045769043292339 2203 12 0.6387385792879527 2203 13 0.3057887629761993 2203 14 0.9033798352540501 2203 15 0.6271762105614016 2203 16 0.5044198593858837 2203 17 0.5445188333024049 2203 18 0.6114584730002619 2203 19 0.47953698628563757 2203 20 0.5161661745504489 2203 21 0.7873174885588838 2203 22 0.7763366761925496 2203 23 0.9423328752528164 2203 24 0.8588947113593761 2203 25 0.7446401348370557 2204 1 0.16442618248051133 2204 2 0.22236103332657464 2204 3 0.08083213171665804 2204 4 -0.14152004155801734 2204 5 0.2345011354131472 2204 6 0.19686216105892385 2204 7 0.3856255583577024 2204 8 0.5026563463030342 2204 9 0.7957165322102853 2204 10 0.7185705296170681 2204 11 0.37729445367161674 2204 12 0.547876737952322 2204 13 0.4261164866254461 2204 14 0.8391160854002777 2204 15 0.49431276486726716 2204 16 0.6013832766695089 2204 17 0.6116186210591437 2204 18 0.5244236951872026 2204 19 0.6540477405012552 2204 20 0.6735696205788244 2204 21 0.6496326113090234 2204 22 0.6260684475046148 2204 23 0.8476364499641136 2204 24 0.6375216478702299 2204 25 0.6226813133720768 2205 1 0.660599293648545 2205 2 0.7724500702159318 2205 3 0.4830882987171492 2205 4 0.20342383588278068 2205 5 0.3263958465910548 2205 6 0.21019609105771764 2205 7 0.01144610196108431 2205 8 0.3695083907566641 2205 9 0.7075476367923699 2205 10 0.7454536521114576 2205 11 0.5729912097660872 2205 12 0.6834627920287275 2205 13 0.5568340591434477 2205 14 0.8652955541416958 2205 15 0.8770973202846264 2205 16 0.5776727684648435 2205 17 0.6579170319744136 2205 18 0.6250538973642037 2205 19 0.7441053907297928 2205 20 0.886960336669965 2205 21 0.7226143132252432 2205 22 1.0377560437555005 2205 23 1.2200397978745983 2205 24 1.0307012986974868 2205 25 1.1582914398137163 2206 1 0.3979674844632761 2206 2 0.5201763904491585 2206 3 0.5125543625361964 2206 4 0.5991091212819768 2206 5 0.9700009406126497 2206 6 0.7013246866532228 2206 7 0.7909404625010836 2206 8 0.7827016986586415 2206 9 1.0402903079416461 2206 10 1.1796683645708868 2206 11 1.0437423423997032 2206 12 0.8785235611489028 2206 13 0.6230407383932288 2206 14 1.0092348553809387 2206 15 0.9583563255248748 2206 16 0.9975203944464326 2206 17 1.1352314743148781 2206 18 1.0473771040404003 2206 19 0.7185947496331622 2206 20 0.9753914135055055 2206 21 1.2828672094328724 2206 22 1.1161647279426816 2206 23 1.3192962824123526 2206 24 1.3909850918652822 2206 25 1.4115914492969424 2207 1 -1.0152554929054887 2207 2 -0.2647431717062112 2207 3 0.0642460206232478 2207 4 0.1771128289392081 2207 5 0.18814477497448223 2207 6 0.15496397013424712 2207 7 0.13588522661954983 2207 8 0.4149298932759038 2207 9 0.7623818182277091 2207 10 0.4822182474038641 2207 11 0.10063703735488616 2207 12 0.27761464824410353 2207 13 -0.10176519385833416 2207 14 -5.402881193658672 2207 15 -2.3511256762310495 2207 16 -1.0276997279631033 2207 17 -0.831611206062334 2207 18 -0.15722620190133085 2207 19 -0.31545079707257884 2207 20 -0.1895756470865848 2207 21 -0.0727883890032146 2207 22 0.10271104839290501 2207 23 0.1518856104231645 2207 24 -0.05072589404309724 2207 25 -0.00444701054098573 2208 1 0.40525067143256166 2208 2 0.8891221491353534 2208 3 0.9277977485104607 2208 4 0.838979557219055 2208 5 0.9384075370570102 2208 6 0.7369238430419642 2208 7 0.7984322670728261 2208 8 0.9337430543339593 2208 9 1.0569517038808982 2208 10 1.299746592485981 2208 11 0.9134167883651614 2208 12 1.0917572887916194 2208 13 0.8390801147814031 2208 14 1.3710596033259037 2208 15 1.3789330979083303 2208 16 1.3078829394042657 2208 17 1.1814332701954484 2208 18 1.375298911509205 2208 19 0.9281183274766462 2208 20 1.2628266628035405 2208 21 1.4304689547439509 2208 22 1.258278282263234 2208 23 1.5471892667424427 2208 24 1.762801369788157 2208 25 1.6125432763227252 2209 1 -0.20815742715049268 2209 2 -0.06592298781625061 2209 3 0.321186066362376 2209 4 0.512897861710023 2209 5 0.4822570291933488 2209 6 0.5259501283809627 2209 7 0.5837086175824027 2209 8 0.7817496904502121 2209 9 1.0590643980211496 2209 10 0.6787631430906487 2209 11 0.7124003607091822 2209 12 0.7647130140371061 2209 13 0.8834996063496945 2209 14 0.9616124755394253 2209 15 0.8578641291442963 2209 16 1.008240657819094 2209 17 1.0574247384906457 2209 18 1.3147669172270966 2209 19 0.9407991612554767 2209 20 0.9378188228796134 2209 21 1.144284106846257 2209 22 1.205217365859781 2209 23 1.2729351100264523 2209 24 1.2167854907323061 2209 25 1.1881648869660353 2210 1 0.10933493376307979 2210 2 -0.04062820625282981 2210 3 0.7114199646363559 2210 4 0.8131713088879238 2210 5 0.9970378585274418 2210 6 0.8680151133780711 2210 7 1.0529733383084192 2210 8 1.0295249499038661 2210 9 1.5883131523683147 2210 10 1.5386040298670025 2210 11 1.1906594748504347 2210 12 1.0422132663792993 2210 13 1.0241055946593676 2210 14 1.4700994317419998 2210 15 1.4607555118877613 2210 16 1.564965347897001 2210 17 1.6491424088675046 2210 18 1.5650545873286745 2210 19 1.5594174271599144 2210 20 1.5688936171060277 2210 21 1.6780236346451929 2210 22 1.7519951854397036 2210 23 2.0271171083931065 2210 24 1.8787900971243658 2210 25 1.9983576764025273 2211 1 -0.8926058853188295 2211 2 -0.07048494192639865 2211 3 0.32312300994088616 2211 4 0.4072400907661091 2211 5 0.7912622809696899 2211 6 0.600598371703903 2211 7 0.750046170591645 2211 8 0.8017159190007774 2211 9 1.00399534558764 2211 10 0.9914018096449766 2211 11 0.7540801919221423 2211 12 0.9494589909517615 2211 13 0.7663948235822602 2211 14 0.8497348952883499 2211 15 0.7625106874053174 2211 16 0.8965903854007493 2211 17 0.889380182644885 2211 18 0.8456351453844775 2211 19 0.9849293533525341 2211 20 0.9612991076550799 2211 21 1.0492957618579126 2211 22 0.8325044863697926 2211 23 1.2971534870309753 2211 24 1.0585230704216926 2211 25 0.9837124618810762 2212 1 1.0776498777455608 2212 2 0.3265616681206893 2212 3 0.689170580498704 2212 4 0.44035514261924646 2212 5 0.787528566320816 2212 6 0.7129131303484968 2212 7 0.9974183071141596 2212 8 0.7040164505315332 2212 9 0.865729645209214 2212 10 0.5607788498275497 2212 11 0.2775106957208564 2212 12 0.4603134844087293 2212 13 0.42004043123167634 2212 14 0.6588279709448202 2212 15 0.6102661254848698 2212 16 0.006911000994243466 2212 17 0.518043483913047 2212 18 0.6186549044623888 2212 19 0.5223486487854103 2212 20 0.5313387558048319 2212 21 0.8516977608757372 2212 22 0.8636687264830343 2212 23 1.326412052186818 2212 24 1.2118220275814693 2212 25 0.8471339744327153 2213 1 0.17352031571590842 2213 2 0.16283915267818472 2213 3 -0.43198138769400174 2213 4 -1.0854844103425378 2213 5 -2.2463393450465716 2213 6 -0.8544090192790961 2213 7 -0.7612019816626052 2213 8 -0.6066820854338104 2213 9 -1.2335092737767326 2213 10 -0.8955161504437115 2213 11 -1.2006731340202066 2213 12 -1.3609622950792826 2213 13 -1.2423819687311166 2213 14 -7.8357787780302 2213 15 -3.6895403103773923 2213 16 -2.442216894717504 2213 17 -1.6513164175029402 2213 18 -6.147158646189801 2213 19 -3.551771496610513 2213 20 -5.594456620356745 2213 21 -2.8952718656365413 2213 22 -2.5418026089738888 2213 23 -5.271975300847817 2213 24 -3.1194148453210992 2213 25 -2.318624936492906 2214 1 0.4702014702437154 2214 2 0.40304578052300855 2214 3 -1.4513114296103353 2214 4 -1.6666726962738387 2214 5 -0.13693841107183147 2214 6 -0.35316067202054313 2214 7 -0.4145201238405676 2214 8 -0.39798655557193996 2214 9 -5.134527643822029 2214 10 -1.5051773032836167 2214 11 -0.7163671447665436 2214 12 -1.0886819338673632 2214 13 -1.175699126297161 2214 14 -2.397158781968649 2214 15 -1.8418984559311653 2214 16 -1.2653348793575603 2214 17 -1.4709073815786695 2214 18 -0.7734667734579261 2214 19 -3.980091748671164 2214 20 -1.954233816744626 2214 21 -3.7564345516578648 2214 22 -5.946428878419972 2214 23 -6.211854193581381 2214 24 -5.474479970366811 2214 25 -3.2600159118213448 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19674.0 3 13.0 4 344.0 5 2218.0 6 2813.0 7 2325.0 8 1668.0 9 1393.0 10 1278.0 11 1209.0 12 1149.0 13 1096.0 14 1108.0 15 1127.0 16 1146.0 17 1306.0 18 1425.0 19 1643.0 20 2157.0 21 2850.0 22 3620.0 23 4407.0 24 5508.0 25 6378.0 26 7417.0 27 8149.0 28 9610.0 29 11736.0 30 14280.0 31 18124.0 32 23649.0 33 30415.0 34 40428.0 35 53906.0 36 73530.0 37 110064.0 38 174368.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.96289706486171 19.155696706523496 9.19283696855831 29.688569260056486 2 12.600837475635801 20.96378088851999 40.6331116851788 25.802269950665412 3 19.867002011446903 24.95468803919679 28.029217276681205 27.149092672675103 4 12.265853419694194 17.53408347283814 36.424666626253014 33.77539648121465 5 12.667400305890252 39.16996182589125 35.24079531465662 12.92184255356188 6 33.25004445193109 36.508916901404056 17.27713985357378 12.963898793091078 7 28.28057434089539 31.06336529133584 21.220435473849925 19.435624893918842 8 25.09466924931022 39.693819717362466 19.345225119737915 15.866285913589397 9 26.73661230775459 15.496572009598061 19.14582856466624 38.6209871179811 10 15.506890276217163 30.151388701097066 33.22298944894886 21.118731573736913 11 34.1395380639587 22.586941285980462 23.804484474964607 19.469036175096228 12 25.033760526368955 24.915501559433288 31.23497481752222 18.81576309667554 13 30.86378123198416 21.78232284070231 24.47030523782071 22.883590689492813 14 20.67785329868805 23.967289131053402 26.214878500487494 29.13997906977106 15 24.469784557242132 31.91626574174219 21.905419013165258 21.708530687850423 16 20.84516278412049 27.85410266331635 30.701872976950245 20.59886157561292 17 21.712402314157977 29.86696544147241 28.288296968067666 20.132335276301948 18 21.948445085375784 27.049857966023733 31.31787017410817 19.683826774492314 19 24.23103340035989 27.14606713637056 26.725105123957693 21.897794339311854 20 24.0256026951514 27.91666537171769 27.779763366204318 20.27796856692659 21 21.65136046493559 27.12849439808556 27.0776809161966 24.142464220782248 22 22.88871864758652 29.593912336779425 25.352780207946466 22.164588807687586 23 20.947242634775947 29.202478202293285 27.56945663845254 22.280822524478232 24 23.652354979565832 28.164965114291483 27.835532143023634 20.347147763119047 25 22.50303404187211 27.698432554142695 27.09069182370391 22.70784158028129 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 277.0 1 277.0 2 270.5 3 264.0 4 264.0 5 264.0 6 358.0 7 452.0 8 452.0 9 452.0 10 653.5 11 855.0 12 855.0 13 855.0 14 1318.0 15 1781.0 16 1781.0 17 1781.0 18 3349.5 19 4918.0 20 4918.0 21 4918.0 22 8610.0 23 12302.0 24 12302.0 25 12302.0 26 19359.5 27 26417.0 28 26417.0 29 26417.0 30 37935.0 31 49453.0 32 49453.0 33 49453.0 34 55667.5 35 61882.0 36 61882.0 37 61882.0 38 65813.5 39 69745.0 40 69745.0 41 69745.0 42 74086.0 43 78427.0 44 78427.0 45 78427.0 46 90815.5 47 103204.0 48 103204.0 49 103204.0 50 95949.0 51 88694.0 52 88694.0 53 88694.0 54 72855.0 55 57016.0 56 57016.0 57 57016.0 58 47694.5 59 38373.0 60 38373.0 61 38373.0 62 31596.0 63 24819.0 64 24819.0 65 24819.0 66 19388.0 67 13957.0 68 13957.0 69 13957.0 70 10169.5 71 6382.0 72 6382.0 73 6382.0 74 4566.0 75 2750.0 76 2750.0 77 2750.0 78 1922.0 79 1094.0 80 1094.0 81 1094.0 82 706.5 83 319.0 84 319.0 85 319.0 86 221.0 87 123.0 88 123.0 89 123.0 90 74.5 91 26.0 92 26.0 93 26.0 94 13.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0874860729319955 2 0.026261361146549273 3 0.033098638604822456 4 0.028592251189142402 5 0.02532900512951202 6 0.3712330874503326 7 0.20729382112128245 8 0.2012335070105403 9 0.20480753840918306 10 0.013674554916546367 11 0.0034186387291365917 12 0.004350994746173844 13 0.0 14 0.06868355992174426 15 0.0029524607206179655 16 0.001553926695062087 17 0.002175497373086922 18 0.0035740313986428003 19 7.769633475310435E-4 20 7.769633475310435E-4 21 1.553926695062087E-4 22 0.0 23 0.0 24 1.553926695062087E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 643531.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.04648734826267 #Duplication Level Percentage of deduplicated Percentage of total 1 90.83999187078565 65.44702325034844 2 6.4710704845699425 9.324357955925686 3 1.2061222921442465 2.606906233842841 4 0.47687257934456734 1.3742797701792704 5 0.26565623900187013 0.9569799431117642 6 0.1528540419861528 0.6607558081251702 7 0.10249547945657331 0.5169107484745488 8 0.07006523468572495 0.403836323547051 9 0.052094110690496256 0.3377877918103651 >10 0.2934914865671842 3.906306833809562 >50 0.03344855170379398 1.660135708060365 >100 0.02649812573218219 4.005191364850392 >500 0.005429943797463451 2.499508563003917 >1k 0.0034751640303766086 4.294998530568366 >5k 4.3439550379707607E-4 2.005021174342277 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 6720 1.0442387390817225 No Hit GTACATGGGAAGCAGTGGTATCAAC 6093 0.9468075353013297 No Hit GAGTACATGGGAAGCAGTGGTATCA 2611 0.40573026008071095 No Hit GTCCTACAGTGGACATTTCTAAATT 2513 0.3905017784691025 No Hit CTGTAGGACGTGGAATATGGCAAGA 2486 0.38630617639243486 No Hit CATGTACTCTGCGTTGATACCACTG 2388 0.3710776947808264 No Hit GCGTTGATACCACTGCTTCCCATGT 1996 0.31016376833439263 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1744 0.271004815618828 No Hit CTTTAGGACGTGAAATATGGCGAGG 1714 0.26634303553364175 No Hit GTATCAACGCAGAGTACATGGGAAG 1676 0.2604381140924058 No Hit ACGCAGAGTACATGGGAAGCAGTGG 1670 0.2595057580753686 No Hit GTCCTACAGTGTGCATTTCTCATTT 1575 0.24474345447227874 No Hit ACTCTGCGTTGATACCACTGCTTCC 1380 0.214441883918568 No Hit CTGTAGGACCTGGAATATGGCGAGA 1302 0.20232125569708376 No Hit GTATCAACGCAGAGTACTTTTTTTT 1175 0.18258638666979524 No Hit GCTTCCCATGTACTCTGCGTTGATA 1105 0.17170889980436063 No Hit TATCAACGCAGAGTACATGGGAAGC 1070 0.16627015637164333 No Hit CTGAAGGACCTGGAATATGGCGAGA 1042 0.16191916162546946 No Hit GGTATCAACGCAGAGTACATGGGAA 993 0.15430492081966526 No Hit GATATACACTGTTCTACAAATCCCG 908 0.1410965439116375 No Hit CAGTGGTATCAACGCAGAGTACATG 830 0.12897591569015324 No Hit GGTATCAACGCAGAGTACTTTTTTT 709 0.11017340267990199 No Hit GCAGTGGTATCAACGCAGAGTACAT 669 0.10395769589965362 No Hit ATACCACTGCTTCCCATGTACTCTG 664 0.1031807325521226 No Hit CATGGGAAGCAGTGGTATCAACGCA 653 0.10147141318755429 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.553926695062087E-4 2 0.0 0.0 0.0 0.0 1.553926695062087E-4 3 0.0 0.0 0.0 0.0 1.553926695062087E-4 4 0.0 0.0 0.0 0.0 1.553926695062087E-4 5 0.0 0.0 0.0 0.0 1.553926695062087E-4 6 0.0 0.0 0.0 0.0 1.553926695062087E-4 7 0.0 0.0 0.0 0.0 1.553926695062087E-4 8 0.0 0.0 0.0 0.0 1.553926695062087E-4 9 0.0 0.0 0.0 0.0 1.553926695062087E-4 10 0.0 0.0 0.0 3.107853390124174E-4 1.553926695062087E-4 11 0.0 0.0 0.0 3.107853390124174E-4 1.553926695062087E-4 12 0.0 0.0 0.0 3.107853390124174E-4 1.553926695062087E-4 13 0.0 0.0 0.0 3.107853390124174E-4 1.553926695062087E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGCTA 40 0.0051213475 14.313469 5 TGCGGCC 40 0.0053545306 14.217749 12 CCAACGA 80 1.3330828E-7 14.2067 19 TAGGACC 545 0.0 14.007064 4 TCCAACG 115 2.7284841E-11 14.001895 18 TATGTCG 55 2.0071972E-4 13.7772665 16 ATAGGAC 120 4.7293724E-11 13.519335 3 CTATACA 65 5.2203963E-5 13.212433 4 GAGTAAG 80 1.9020918E-6 13.117599 1 AAGACTC 60 3.9357998E-4 12.7230835 5 GACAGCC 60 4.0838053E-4 12.667549 7 ATGTCGA 60 4.189731E-4 12.62916 17 TGTCGAG 60 4.189731E-4 12.62916 18 TGTAGGA 1300 0.0 12.55476 2 GGCGAGG 480 0.0 12.430863 19 GTATAAA 85 3.752244E-6 12.345976 1 CTGTAGG 1345 0.0 12.341804 1 GTCCTAA 535 0.0 12.304018 1 GTAGGAC 1285 0.0 12.253734 3 TGGCGAG 1240 0.0 12.145383 18 >>END_MODULE