FastQCFastQC Report
Thu 26 May 2016
SRR1926541_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926541_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences546540
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC22100.404361986313902No Hit
GTACATGGGAAGCAGTGGTATCAAC20050.3668532952757346No Hit
GAGTACATGGGAAGCAGTGGTATCA10410.19047096278405973No Hit
CATGTACTCTGCGTTGATACCACTG8020.14674131811029384No Hit
GCGTTGATACCACTGCTTCCCATGT7610.1392395799026604No Hit
ACGCAGAGTACATGGGAAGCAGTGG6900.1262487649577341No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACTG304.3793052E-420.6640035
GTTTTGG350.00610379318.9555131
CTACAAG358.782266E-418.6349323
CTATACA801.6589183E-917.3238894
TAGGACC1450.016.910324
GGTCTGG552.442132E-416.887641
TCTAGGA757.221919E-616.2850362
CCAACGA602.454217E-715.72284919
TGTAGGA3350.014.9887152
TCCAACG1051.8189894E-1214.71088918
AGAACAG1650.014.40218455
GAACAGT1400.014.0185566
TGTAGAA2050.013.901862
GCCGGTT603.5390254E-513.7686711
CTAGGAC807.1384657E-613.5879733
CTCTATA700.001284014313.5708632
TCGTTGA400.007308779313.56429513
GTAGGAC3950.013.4847723
CTGTAGG3750.013.4457791
CCCTGTA956.721491E-512.8566072
Produced by FastQC (version 0.11.5)