FastQCFastQC Report
Thu 26 May 2016
SRR1926541_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926541_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences546540
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC60701.1106231931789072No Hit
GTACATGGGAAGCAGTGGTATCAAC56841.0399970724924068No Hit
GAGTACATGGGAAGCAGTGGTATCA27810.5088374135470414No Hit
CATGTACTCTGCGTTGATACCACTG22060.40363010941559635No Hit
GCGTTGATACCACTGCTTCCCATGT18840.34471401910198707No Hit
GTATCAACGCAGAGTACATGGGAAG17170.31415815859772384No Hit
ACGCAGAGTACATGGGAAGCAGTGG17070.3123284663519596No Hit
ACTCTGCGTTGATACCACTGCTTCC13360.24444688403410544No Hit
CTGTAGGACGTGGAATATGGCAAGA12320.22541808467815713No Hit
GTCCTACAGTGGACATTTCTAAATT12120.22175870018662863No Hit
GCTTCCCATGTACTCTGCGTTGATA11840.21663556189848868No Hit
TATCAACGCAGAGTACATGGGAAGC10420.19065393200863615No Hit
GATATACACTGTTCTACAAATCCCG10040.18370110147473195No Hit
GTATCAACGCAGAGTACTTTTTTTT9810.17949280930947414No Hit
GGTATCAACGCAGAGTACATGGGAA9680.1771142093899806No Hit
CTTTAGGACGTGAAATATGGCGAGG9190.16814871738573572No Hit
GTCCTAAAGTGTGTATTTCTCATTT8510.15570681011453874No Hit
CAGTGGTATCAACGCAGAGTACATG8430.1542430563179273No Hit
GTCCTACAGTGTGCATTTCTCATTT7210.1319208109196033No Hit
CATGGGAAGCAGTGGTATCAACGCA6860.1255168880594284No Hit
GTGGTATCAACGCAGAGTACATGGG6830.12496798038569912No Hit
ATACCACTGCTTCCCATGTACTCTG6810.12460204193654627No Hit
ACATGGGAAGCAGTGGTATCAACGC6250.11435576536026641No Hit
GGTATCAACGCAGAGTACTTTTTTT6220.11380685768653713No Hit
GCAGTGGTATCAACGCAGAGTACAT5940.10868371939839719No Hit
GTGTATATCAATGAGTTACAATGAA5800.10612215025432722No Hit
CTGTAGGACCTGGAATATGGCGAGA5760.10539027335602152No Hit
GGGAAGCAGTGGTATCAACGCAGAG5490.100450104292458No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCGT250.005900178619.070166
TTAGACA504.382726E-617.1678874
CCAACGA1450.015.68256919
TAGACTG508.414894E-515.2589385
CACTATA400.005120760814.3131622
ATCCCGT1350.014.04586210
TTTAGAC551.8888363E-413.8755953
TAGGACC1800.013.7766994
TCCAACG1800.013.68594618
TCATATA500.001448269713.35895252
GGTCATA500.001451290813.3552611
GGCGAGG1850.013.31605419
GAGAGCG500.001498296413.2989097
GTAGGAC6250.013.2789453
GACGTGG3600.012.9294957
CGTGGAA3700.012.5765889
TGTAGGA6750.012.4400822
CGTTTCC1457.2759576E-1212.42218714
TTACAAT2450.012.375360516
CCCGTTT1551.8189894E-1212.23349512
Produced by FastQC (version 0.11.5)