FastQCFastQC Report
Thu 26 May 2016
SRR1926540_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926540_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628541
Sequences flagged as poor quality0
Sequence length25
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC24580.3910643856168492No Hit
CCCATGTACTCTGCGTTGATACCAC23950.3810411731295174No Hit
GAGTACATGGGAAGCAGTGGTATCA12060.19187292475749393No Hit
CATGTACTCTGCGTTGATACCACTG8680.13809759426990442No Hit
GCGTTGATACCACTGCTTCCCATGT8290.13189274844441334No Hit
GTATCAACGCAGAGTACATGGGAAG6840.10882344986245926No Hit
ACGCAGAGTACATGGGAAGCAGTGG6780.10786885819699908No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGT200.00947447126.4708081
GCTCTAG350.006195197318.9077191
GTCCTAC901.7724415E-716.1766031
GTCTTAT500.002560525715.8824841
TCCTAGA707.331927E-515.5044362
GTATATG709.0530426E-515.1261751
TCTCCGT758.846655E-814.6895610
CTACTAT550.004828549514.43862151
GTATAAG658.867179E-414.253511
CTCTCCG852.6768248E-814.0414929
CTACACC550.00127783813.5817814
TACTATA802.2830658E-413.56638052
CACCGTG602.3503343E-413.5217397
GTCCTAG802.8143756E-413.2354041
TCCGTTG854.885187E-712.76835912
TGTAGAA2550.012.7683582
GTAGAAC2400.012.6869083
TAGAACA2450.012.6314534
GATATAC2005.456968E-1212.5736331
CTAGACC600.002459371512.4499664
Produced by FastQC (version 0.11.5)