FastQCFastQC Report
Thu 26 May 2016
SRR1926540_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926540_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628541
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC67151.068347172260839No Hit
GTACATGGGAAGCAGTGGTATCAAC63961.01759471538054No Hit
GAGTACATGGGAAGCAGTGGTATCA28440.4524764494281201No Hit
CATGTACTCTGCGTTGATACCACTG25620.40761064115149215No Hit
GCGTTGATACCACTGCTTCCCATGT21690.34508488706385104No Hit
ACGCAGAGTACATGGGAAGCAGTGG18480.294014232961732No Hit
GTATCAACGCAGAGTACATGGGAAG16810.2674447649397573No Hit
ACTCTGCGTTGATACCACTGCTTCC14330.2279883094340703No Hit
TATCAACGCAGAGTACATGGGAAGC11170.17771314838650143No Hit
GCTTCCCATGTACTCTGCGTTGATA11100.17659945811013122No Hit
GTATCAACGCAGAGTACTTTTTTTT10680.16991731645191005No Hit
GGTATCAACGCAGAGTACATGGGAA10150.16148509007367856No Hit
GATATACACTGTTCTACAAATCCCG9310.1481208067572362No Hit
CAGTGGTATCAACGCAGAGTACATG9200.1463707220372259No Hit
CATGGGAAGCAGTGGTATCAACGCA7640.1215513387352615No Hit
GTGGTATCAACGCAGAGTACATGGG7160.11391460541158015No Hit
ATACCACTGCTTCCCATGTACTCTG7080.11264181652429993No Hit
GCAGTGGTATCAACGCAGAGTACAT6980.11105083041519964No Hit
GGTATCAACGCAGAGTACTTTTTTT6940.11041443597155952No Hit
GTGTATATCAATGAGTTACAATGAA6480.10309589986969823No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGCT508.423903E-515.257714
TCTACAC1102.579327E-913.0047713
TTATCAT1403.6379788E-1212.85944218
GATATAC3000.012.391931
TCCAACG1155.506081E-912.35918818
GTCTAGG550.002988090312.1319581
GTATAGA550.002988090312.1319581
CGGTTTC802.917887E-511.85176613
TATACAC3450.011.6100573
ACTGCGA600.005867566411.0837058
TAGGACA1657.4578566E-1110.9809274
CTAATAT1053.301424E-610.8983644
GACGTGA1053.4534678E-610.8575087
ACGTGAA1158.779025E-710.73949058
CAGGACC803.6343365E-410.7280784
ATACACT4000.010.7280784
TGTAGAA3500.010.6284582
GCCGGTT909.68383E-510.53490211
CCGGTTT909.68383E-510.53490212
CCAACGA909.746544E-510.52819719
Produced by FastQC (version 0.11.5)