FastQCFastQC Report
Thu 26 May 2016
SRR1926539_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926539_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences554499
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC28280.5100099368979926No Hit
GTACATGGGAAGCAGTGGTATCAAC26690.48133540367070093No Hit
GAGTACATGGGAAGCAGTGGTATCA13130.23679032784549656No Hit
CATGTACTCTGCGTTGATACCACTG10330.18629429448925966No Hit
GCGTTGATACCACTGCTTCCCATGT9560.17240788531629453No Hit
ACGCAGAGTACATGGGAAGCAGTGG8450.15238981495007206No Hit
GTCCTACAGTGGACATTTCTAAATT7500.13525723220420593No Hit
GTATCAACGCAGAGTACATGGGAAG7500.13525723220420593No Hit
CNCATGTACTCTGCGTTGATACCAC6710.12101013707869626No Hit
ACTCTGCGTTGATACCACTGCTTCC6310.1137964180278053No Hit
GCTTCCCATGTACTCTGCGTTGATA6260.11289470314644391No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTACG306.7258065E-527.2391722
GTCCTAG752.1069354E-819.487731
TTTTGGA350.005249487719.4565532
TCTAAGC450.001044093518.1594492
GTTATAA450.001232498817.7161181
GACTGTC350.001387132917.4082747
CTAGGAC606.951146E-616.3529473
GTCTAGC500.002493887715.9445071
GTCTAAG500.002493887715.9445071
CTTGGAC701.7337843E-615.5742363
TCTAGGA707.086392E-515.5652422
CCTAGAG804.210142E-714.9902013
TTCTAGA550.003990846714.857732
GTCCTAA1353.8198777E-1014.76343251
CTACACT805.129641E-714.7310654
TGTAGGA3900.014.6672462
AGGACGG507.58661E-414.55089955
CTTAAAC601.2709913E-414.53595353
TCCTAGA852.2837432E-514.4207392
CTGTAGG3600.014.3943461
Produced by FastQC (version 0.11.5)