FastQCFastQC Report
Thu 26 May 2016
SRR1926539_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926539_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences554499
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC88081.588460935006195No Hit
GTACATGGGAAGCAGTGGTATCAAC78741.4200205951678904No Hit
GAGTACATGGGAAGCAGTGGTATCA36410.6566287766073519No Hit
CATGTACTCTGCGTTGATACCACTG32260.5817864414543579No Hit
GCGTTGATACCACTGCTTCCCATGT25670.46294042009092895No Hit
ACGCAGAGTACATGGGAAGCAGTGG23560.42488805209747904No Hit
GTATCAACGCAGAGTACATGGGAAG21640.3902622006532022No Hit
ACTCTGCGTTGATACCACTGCTTCC18710.3374217086054258No Hit
CTGTAGGACGTGGAATATGGCAAGA18110.32660113002908936No Hit
GCTTCCCATGTACTCTGCGTTGATA17630.3179446671680201No Hit
GTCCTACAGTGGACATTTCTAAATT16710.30135311335097087No Hit
TATCAACGCAGAGTACATGGGAAGC14160.25536565440154085No Hit
GGTATCAACGCAGAGTACATGGGAA13590.2450861047540212No Hit
GTATCAACGCAGAGTACTTTTTTTT12210.22019877402844729No Hit
CTTTAGGACGTGAAATATGGCGAGG12070.21767397236063546No Hit
CAGTGGTATCAACGCAGAGTACATG11300.2037875631876703No Hit
GTCCTAAAGTGTGTATTTCTCATTT10430.1880977242519824No Hit
CATGGGAAGCAGTGGTATCAACGCA9320.16807965388575993No Hit
GTCCTACAGTGTGCATTTCTCATTT8770.15816079019078483No Hit
GTGGTATCAACGCAGAGTACATGGG8750.15780010423824029No Hit
GATATACACTGTTCTACAAATCCCG8740.157619761261968No Hit
ACATGGGAAGCAGTGGTATCAACGC8690.15671804638060663No Hit
ATACCACTGCTTCCCATGTACTCTG8690.15671804638060663No Hit
GCAGTGGTATCAACGCAGAGTACAT8600.15509495959415617No Hit
GGTATCAACGCAGAGTACTTTTTTT8470.1527505009026166No Hit
CTGTAGGACCTGGAATATGGCGAGA8220.14824192649580972No Hit
CCATGTACTCTGCGTTGATACCACT7860.14174957935000784No Hit
GGGAAGCAGTGGTATCAACGCAGAG7530.13579826113302276No Hit
GTGTATATCAATGAGTTACAATGAA7440.1341751743465723No Hit
CCACTGCTTCCCATGTACTCTGCGT7410.1336341454177555No Hit
CTGCTTCCCATGTACTCTGCGTTGA7050.1271417982719536No Hit
AAGCAGTGGTATCAACGCAGAGTAC6940.12515802553295857No Hit
GTACTCTGCGTTGATACCACTGCTT6880.12407596767532493No Hit
CTGAAGGACCTGGAATATGGCGAGA6810.122813566841419No Hit
GATATACACTGTTCTACAATGCCGG6770.12209219493632992No Hit
TACCACTGCTTCCCATGTACTCTGC6520.11758362052952305No Hit
GATACCACTGCTTCCCATGTACTCT6490.11704259160070622No Hit
CTTCCCATGTACTCTGCGTTGATAC6250.11271436017017164No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6240.11253401719389935No Hit
TATCAACGCAGAGTACTTTTTTTTT6010.10838612873963703No Hit
GTGTATATCAATGAGTTACAATGAG5860.10568098409555293No Hit
GCAGAGTACATGGGAAGCAGTGGTA5800.10459892623791928No Hit
ATGGGAAGCAGTGGTATCAACGCAG5560.10027069480738468No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTATA453.4276432E-516.9481642
TAGGACC2700.014.8256084
TCCAACG1600.014.80718118
GTGTAGC1400.014.2896421
GTGTAGA759.342875E-713.9720951
TTGTAGA1550.013.5213811
GTAGCCC852.5817826E-713.4563953
CCAACGA1205.2750693E-1113.42517919
TAAAGCA500.001461383213.3430474
ACAATTC500.00149570113.3020268
CGAAATC655.520563E-513.13328613
TGTAGGA8750.013.0742982
CTGTAGG8550.013.0361651
GTAGGAC7900.012.9099063
AGAACAG2400.012.70766455
ACGTTTT604.1705227E-412.63546219
CCAACGT604.1705227E-412.63546219
TGTAGAA3700.012.6252382
GCCGGTT1001.4654506E-712.33069511
CCGGTTT1001.4654506E-712.33069512
Produced by FastQC (version 0.11.5)