FastQCFastQC Report
Thu 26 May 2016
SRR1926538_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926538_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences560916
Sequences flagged as poor quality0
Sequence length25
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT11420.2035955472833722No Hit
CTGTAGGACGTGGAATATGGCAAGA9190.16383914882085732No Hit
GTACATGGGAAGCAGTGGTATCAAC8650.15421203887926177No Hit
CCCATGTACTCTGCGTTGATACCAC8620.15367719943806202No Hit
GTCCTAAAGTGTGTATTTCTCATTT7760.13834513545700247No Hit
CTTTAGGACGTGAAATATGGCGAGG6850.12212167240727666No Hit
GTCCTACAGTGTGCATTTCTCATTT6160.10982036525968239No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGG350.006046626718.9865911
GTGTAGA500.00248958815.9487361
TGTAGGA7300.015.4810922
TAGGACC2550.015.4809844
GAGTGTC400.00343384315.1990887
CTGTAGG7150.015.05649951
CCTAAAC450.00468695514.5039313
GTGTTAA550.004695432714.4988511
GTACCAA550.004695432714.4988511
GTATAAG550.004695432714.4988511
GATATAC3050.014.3800081
GTTTAAG658.588596E-414.3129671
TAATGGT450.005330000514.2257694
GTAGGAC7500.013.7787363
TAGGACA2350.013.6204184
TCTTATA600.007120837513.61592
TGTACAG851.5002697E-613.3946855
TGTAGAA2550.013.3489232
GTCTTAC600.0083560813.2906131
GTCCTAC8100.013.1265321
Produced by FastQC (version 0.11.5)