FastQCFastQC Report
Thu 26 May 2016
SRR1926538_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926538_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences560916
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA28400.5063146710024318No Hit
CCCATGTACTCTGCGTTGATACCAC26320.4692324697459156No Hit
GTCCTACAGTGGACATTTCTAAATT25710.45835740110818735No Hit
GTACATGGGAAGCAGTGGTATCAAC24940.44462985545072703No Hit
CTTTAGGACGTGAAATATGGCGAGG20720.3693957740552953No Hit
GTCCTAAAGTGTGTATTTCTCATTT19150.34140584329917495No Hit
GTCCTACAGTGTGCATTTCTCATTT15350.2736595140805397No Hit
CTGTAGGACCTGGAATATGGCGAGA13710.2444216246282866No Hit
GTATCAACGCAGAGTACTTTTTTTT12800.2281981615785608No Hit
GAGTACATGGGAAGCAGTGGTATCA12160.21678825349963274No Hit
CTGAAGGACCTGGAATATGGCGAGA11830.21090501964643546No Hit
GATATACACTGTTCTACAAATCCCG11300.20145618951857316No Hit
CATGTACTCTGCGTTGATACCACTG9660.1722183000663201No Hit
GCGTTGATACCACTGCTTCCCATGT8700.15510343794792805No Hit
GTATCAACGCAGAGTACATGGGAAG8130.14494148856513275No Hit
GGTATCAACGCAGAGTACTTTTTTT8010.14280213080033374No Hit
ACGCAGAGTACATGGGAAGCAGTGG7920.14119761247673449No Hit
GTGTATATCAATGAGTTACAATGAG7740.13798857582953597No Hit
GTGTATATCAATGAGTTACAATGAA7360.13121394290767247No Hit
GAATATGGCAAGAAAACTGAAAATC7170.1278266264467407No Hit
GATATACACTGTTCTACAATGCCGG6470.11534703948541314No Hit
ACCTGGAATATGGCGAGAAAACTGA6380.11374252116181388No Hit
ACAGTGGACATTTCTAAATTTTCCA6190.11035520470088214No Hit
GTCCTTCAGTGTGCATTTCTCATTT6100.10875068637728287No Hit
CTGTAGGACATGGAATATGGCAAGA6040.10768100749488337No Hit
ACTCTGCGTTGATACCACTGCTTCC6020.10732444786741688No Hit
TATCAACGCAGAGTACTTTTTTTTT5970.10643304879875061No Hit
GCCATATTCCACGTCCTACAGTGGA5890.1050068102888846No Hit
GTTCTACAGTGTGGTTTTTATCATT5850.10429369103395161No Hit
CTGTAGAACATATTAGATGAGTGAG5840.10411541122021836No Hit
ATTCCAGGTCCTTCAGTGTGCATTT5840.10411541122021836No Hit
TTGTAGAACAGTGTATATCAATGAG5760.10268917271035236No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGCCG402.6929117E-416.6764475
TAGGACC5200.014.8438714
CCAACGA1203.6379788E-1214.21637119
GATATAC4100.013.2481871
CCTACAC801.9229665E-613.1040983
GGACCTG8150.013.0956156
TGTAGGA14200.012.9565742
ATAGGAC1550.012.9119553
AGGACCT8600.012.8536075
CTGTAGG14050.012.2763071
GGACATC550.002994109612.1283256
GTGCCCC550.003100250112.0720611
GTAGGAC14100.012.0310993
GACGTGG7150.011.9618117
TGTCGAG658.1697217E-411.66471518
TCCAACG1950.011.66471418
CTACACT1406.4028427E-1011.5714124
CACTATA752.0040419E-411.439382
AGTACTG752.0107313E-411.4352795
GTGTAGG1251.7394996E-811.4352781
Produced by FastQC (version 0.11.5)