FastQCFastQC Report
Thu 26 May 2016
SRR1926537_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926537_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences624367
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC19910.31888296466661437No Hit
CCCATGTACTCTGCGTTGATACCAC19500.3123163139627815No Hit
GAGTACATGGGAAGCAGTGGTATCA9220.14766955973009463No Hit
CATGTACTCTGCGTTGATACCACTG7310.11707857718297092No Hit
GCGTTGATACCACTGCTTCCCATGT6830.10939079099311783No Hit
GTCCTACAGTGGACATTTCTAAATT6600.1057070601104799No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTGT501.1748485E-418.5838051
CCAACGA506.3384687E-717.15293119
CCTAGGC452.4014505E-416.9273263
GTCCTAC5200.016.337411
TGTAGGA4100.016.234362
AAATAGC400.002948542115.5512086
TCCTAGA707.2515795E-515.5244032
CGCAGGG455.686576E-415.1157648
TCCTACA5950.014.8395022
GTATTGT550.00473564714.4808871
TAGGACA1550.014.4694364
CTGTAGG4100.014.2454261
TAAACCT450.005296581414.2397624
TCCAACG802.9307557E-813.94547318
CCTACAG7050.013.2743183
CCTGTAC1108.452116E-812.8603723
CCTAGAC600.002123784312.6954973
TCAGCAC600.002123784312.6954973
GTAGGAC4500.012.5745863
TAGGACC1554.0017767E-1112.4023734
Produced by FastQC (version 0.11.5)