##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926537_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 624367 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 19.706744590921687 26.0 2.0 30.0 2.0 31.0 2 15.596677915392709 16.0 2.0 30.0 2.0 31.0 3 18.986877909947193 19.0 10.0 30.0 2.0 31.0 4 21.08548017432055 27.0 2.0 35.0 2.0 35.0 5 21.68467103482407 28.0 2.0 35.0 2.0 37.0 6 22.005469539549654 29.0 2.0 35.0 2.0 36.0 7 21.971994035559213 29.0 2.0 35.0 2.0 37.0 8 22.021386460206898 29.0 2.0 35.0 2.0 37.0 9 24.54821923644267 31.0 10.0 37.0 2.0 39.0 10 25.180038342833623 31.0 8.0 37.0 2.0 39.0 11 22.471360594009614 29.0 2.0 35.0 2.0 39.0 12 23.50386391337146 30.0 2.0 35.0 2.0 39.0 13 20.45319980075821 25.0 2.0 35.0 2.0 39.0 14 23.475186869261186 29.0 2.0 37.0 2.0 40.0 15 24.44849583658329 30.0 2.0 37.0 2.0 40.0 16 24.780409598841707 31.0 2.0 38.0 2.0 40.0 17 24.09050606454217 30.0 2.0 37.0 2.0 40.0 18 24.086876788811708 30.0 2.0 37.0 2.0 40.0 19 24.449469622834005 30.0 2.0 38.0 2.0 40.0 20 24.66004769630682 31.0 2.0 38.0 2.0 40.0 21 22.606271631908797 30.0 2.0 36.0 2.0 40.0 22 23.47605334682967 30.0 2.0 37.0 2.0 40.0 23 23.417533277703658 30.0 2.0 37.0 2.0 40.0 24 22.624573367907015 30.0 2.0 38.0 2.0 40.0 25 22.401896640917922 30.0 2.0 37.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.203601552335499 1101 2 9.720216654135037 1101 3 6.628084306318897 1101 4 8.108615326978512 1101 5 8.167330493472122 1101 6 7.249980690769188 1101 7 7.102985030750194 1101 8 5.42038309979791 1101 9 5.498510221114024 1101 10 5.291617446351516 1101 11 7.997692209964708 1101 12 5.7250397821679755 1101 13 9.746207914547835 1101 14 7.564443285071938 1101 15 6.643385728229866 1101 16 5.992192399128033 1101 17 2.140838234399286 1101 18 4.610128309131966 1101 19 5.253087901240502 1101 20 3.15893275949718 1101 21 0.1272701281228521 1101 22 3.916429356050962 1101 23 5.146013431574779 1101 24 6.592167406957898 1101 25 7.064652102562292 1102 1 5.35001199912865 1102 2 9.892471120037246 1102 3 6.861981632149309 1102 4 8.094995787547184 1102 5 7.995438918184654 1102 6 6.714309577896678 1102 7 6.859753617155963 1102 8 6.808683219886053 1102 9 6.1221321718579915 1102 10 5.666167513717067 1102 11 7.894979841363341 1102 12 5.715639641945767 1102 13 9.849753499100157 1102 14 7.806915493301119 1102 15 5.589378691653515 1102 16 6.345475166519126 1102 17 6.96428240057454 1102 18 6.933494087751182 1102 19 5.850300010009004 1102 20 5.973407320148855 1102 21 4.9062060787251625 1102 22 5.7644100209843465 1102 23 6.256232701862235 1102 24 7.537206986705101 1102 25 7.329523256498742 1103 1 5.00939511790817 1103 2 8.627350136970314 1103 3 6.456651352097172 1103 4 8.075206268719015 1103 5 7.382912784065919 1103 6 7.050152106679217 1103 7 7.173764705303455 1103 8 6.735630106715419 1103 9 5.812737011180669 1103 10 5.027434849449179 1103 11 5.785168135038425 1103 12 4.6803060733753625 1103 13 8.323928229037595 1103 14 7.045218649878599 1103 15 6.30722062354198 1103 16 6.0410215846346205 1103 17 3.8488322056238147 1103 18 2.942869965389498 1103 19 4.957044832993109 1103 20 5.254616438705938 1103 21 7.279567320456859 1103 22 3.222988692983055 1103 23 5.462599625289368 1103 24 6.620188064522964 1103 25 2.542055151525396 1104 1 5.723068985867506 1104 2 10.201506911401907 1104 3 7.387746050368097 1104 4 7.855333054728661 1104 5 7.912350930560059 1104 6 7.518589197153407 1104 7 7.53767967661279 1104 8 7.3743877883063185 1104 9 6.422728486682278 1104 10 5.48489108187535 1104 11 8.13121267236173 1104 12 5.079348021541321 1104 13 9.137150266774402 1104 14 7.47758566134214 1104 15 6.511211392909768 1104 16 6.278102039359357 1104 17 6.833145379154612 1104 18 4.843119020696953 1104 19 5.1781983121335315 1104 20 4.418146058157063 1104 21 3.4492127903857366 1104 22 5.612980518370794 1104 23 6.151353648979839 1104 24 6.924410076813508 1104 25 6.1445876909353 1105 1 3.435763010354627 1105 2 9.16683153596216 1105 3 6.436067762196814 1105 4 5.786910658218893 1105 5 2.7754178729484984 1105 6 4.853209722453823 1105 7 5.9320789730179015 1105 8 2.9694857935265375 1105 9 4.010387835793956 1105 10 4.068669153167924 1105 11 7.025005937773361 1105 12 4.156863247183448 1105 13 4.9189704402773735 1105 14 3.6344412982972614 1105 15 4.517832025388614 1105 16 4.797889857584735 1105 17 5.326305661349121 1105 18 3.114846295716813 1105 19 2.8849707281876 1105 20 1.902339603493541 1105 21 2.7016700135816727 1105 22 4.2056796180719545 1105 23 1.4265500376971012 1105 24 4.595737166477726 1105 25 5.5230315861778365 1106 1 6.7351253854000745 1106 2 11.003142928377123 1106 3 8.071874840576598 1106 4 9.220621510353237 1106 5 8.904333832337535 1106 6 8.13509390223982 1106 7 7.907162732151264 1106 8 7.70228848945645 1106 9 6.901251151052779 1106 10 6.533306574833098 1106 11 8.604745023674852 1106 12 5.521349374638067 1106 13 4.57660610661695 1106 14 5.953528216234815 1106 15 6.434697615924712 1106 16 6.470543917703658 1106 17 6.859036424114326 1106 18 6.906337853343487 1106 19 6.5111708196678215 1106 20 6.145905299192798 1106 21 6.007477443808455 1106 22 6.743276815806059 1106 23 7.245467678132922 1106 24 7.6567083423895 1106 25 6.571271373818625 1107 1 3.6653352940799344 1107 2 -12.812577595640345 1107 3 -5.040587012669116 1107 4 1.5626662924187507 1107 5 4.177015534659411 1107 6 4.423786991537071 1107 7 4.392083112284393 1107 8 4.98848835717569 1107 9 3.2975086111848277 1107 10 2.7919425358605565 1107 11 5.227132188573261 1107 12 2.648323432911223 1107 13 3.812552436212645 1107 14 3.5633371053471805 1107 15 3.2705124383319912 1107 16 3.0762103303809525 1107 17 3.7552478567304313 1107 18 -0.8787291186261434 1107 19 1.5137189648362863 1107 20 1.9388634760390246 1107 21 -1.0716504314608422 1107 22 1.6563497906920546 1107 23 -1.148550291937589 1107 24 1.8277295730647118 1107 25 2.6262460200062705 1108 1 5.220402989891653 1108 2 9.987422404359656 1108 3 0.5985357887986815 1108 4 5.692229432998278 1108 5 7.0141240693930555 1108 6 6.961509217274131 1108 7 7.142646980435728 1108 8 6.869810323517587 1108 9 5.441226977627952 1108 10 4.669020860547384 1108 11 6.71165784338789 1108 12 2.951920959167076 1108 13 3.204428938806597 1108 14 4.831924192529261 1108 15 4.800371352668318 1108 16 4.757314816019317 1108 17 0.008510067283385325 1108 18 3.316018920093331 1108 19 4.380474469075182 1108 20 4.808865374052434 1108 21 4.7195095710698425 1108 22 1.6788507396987598 1108 23 4.570682119804786 1108 24 5.344379895726991 1108 25 5.746587187917189 1109 1 2.653514426451366 1109 2 -12.812577595640345 1109 3 -5.338528184041154 1109 4 1.2301821024164639 1109 5 0.5845382951952125 1109 6 1.419534946205033 1109 7 2.4860425003398703 1109 8 3.0480838556704306 1109 9 2.519318277055458 1109 10 1.785869482568419 1109 11 -0.7377172398234002 1109 12 0.24836866919237366 1109 13 1.1369279402711143 1109 14 2.007241227544572 1109 15 2.287779820249199 1109 16 2.192900124233933 1109 17 2.7320114319485427 1109 18 2.8713585885795503 1109 19 1.4952769254724672 1109 20 0.2177822959003919 1109 21 -0.18498217437018027 1109 22 1.854989070147436 1109 23 2.323465537458894 1109 24 3.302443069738441 1109 25 3.5883251389223823 1110 1 2.852870522359183 1110 2 8.085986997661092 1110 3 5.601483498983235 1110 4 6.99746695863054 1110 5 7.85443934622149 1110 6 3.4914007073629882 1110 7 6.443975586040651 1110 8 7.027166782847729 1110 9 5.319520723355907 1110 10 5.061536239904868 1110 11 2.750170317755625 1110 12 5.40073760304951 1110 13 9.463348966147677 1110 14 7.360431540444505 1110 15 6.641605118902085 1110 16 6.329619156415763 1110 17 6.633813382389331 1110 18 6.813870082089231 1110 19 5.56904675205536 1110 20 5.838786768447516 1110 21 3.8054716353213784 1110 22 5.824014786178598 1110 23 6.447343944821878 1110 24 6.914860073444011 1110 25 7.3678406055521855 1111 1 5.242870522359183 1111 2 9.231967858905108 1111 3 7.254737087499979 1111 4 7.757132030400875 1111 5 8.237549394068381 1111 6 7.789869606884523 1111 7 7.799286590825339 1111 8 7.951855778063042 1111 9 6.4076068477578225 1111 10 5.712397483924008 1111 11 7.1468688823489295 1111 12 6.664469660465777 1111 13 8.52741595179361 1111 14 6.435024841879912 1111 15 6.107968755265723 1111 16 5.922298582253081 1111 17 7.038071755595073 1111 18 6.75808060840502 1111 19 5.792730962581675 1111 20 5.535293945481008 1111 21 8.116619960680232 1111 22 7.558655934503953 1111 23 7.28586069123336 1111 24 7.175386389233484 1111 25 7.4634865385665385 1112 1 -1.8699859062717614 1112 2 6.86661580490961 1112 3 5.486153636120928 1112 4 -0.2072484378934334 1112 5 3.5107907906186675 1112 6 1.9846850389318504 1112 7 5.127697556578195 1112 8 5.540995016459842 1112 9 4.107840994912223 1112 10 3.118091176116323 1112 11 -0.19598504649033544 1112 12 3.6820448625322726 1112 13 7.429648265767444 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3.668012498782989 2204 7 4.877468409007157 2204 8 4.952671608084938 2204 9 3.5186124434110546 2204 10 3.5163574222907243 2204 11 4.8353540011551885 2204 12 5.069993948844662 2204 13 2.7604376045387227 2204 14 4.379229691446046 2204 15 4.552853192862024 2204 16 4.403368244080994 2204 17 -0.5189884990476976 2204 18 2.7569079541190717 2204 19 3.697000689286728 2204 20 4.102528557434621 2204 21 5.49855411038423 2204 22 5.024533803289941 2204 23 3.9744869178167654 2204 24 5.170200272941212 2204 25 5.373322723790771 2205 1 5.615532289414624 2205 2 9.599548942145244 2205 3 7.026357154603353 2205 4 7.194379194484593 2205 5 7.616768119104329 2205 6 6.9894733774549 2205 7 7.145482468761113 2205 8 6.663285718948167 2205 9 5.88804941046752 2205 10 5.150178279578267 2205 11 7.075402194379603 2205 12 6.489246781411616 2205 13 9.27221703822914 2205 14 7.117100253287486 2205 15 6.024957092060738 2205 16 5.593928235552333 2205 17 6.651861498365484 2205 18 6.281097064707943 2205 19 4.805664338520323 2205 20 5.202130500841289 2205 21 -0.7633017520534331 2205 22 3.9312170465026774 2205 23 5.294735910917016 2205 24 6.807827673783731 2205 25 7.126437489197631 2206 1 3.7074002758526667 2206 2 8.222828276553091 2206 3 5.642611165063176 2206 4 1.7682687865635387 2206 5 5.345765754564535 2206 6 5.6028104139185935 2206 7 5.491458046312857 2206 8 5.543147974640213 2206 9 4.757691633438462 2206 10 3.9909922397726483 2206 11 3.4579522549348916 2206 12 4.367010090674601 2206 13 7.453010236610691 2206 14 0.7603522088134973 2206 15 2.621806092367308 2206 16 3.414986602350428 2206 17 4.528903911349509 2206 18 -0.671898980227418 2206 19 0.9896143037655349 2206 20 2.614965793494825 2206 21 3.0793880561426263 2206 22 -1.8415230574885086 2206 23 -2.7624121930498866 2206 24 0.9439450326625938 2206 25 2.2886254928764806 2207 1 1.0107916453538373 2207 2 -12.812577595640345 2207 3 -6.052278286831571 2207 4 -4.478332541791637 2207 5 0.25973856519137684 2207 6 0.7733789651732899 2207 7 1.810372820771864 2207 8 1.931942676458764 2207 9 -0.20488646775019959 2207 10 -0.26173018987278596 2207 11 -1.7821184171163118 2207 12 -3.1459782004967884 2207 13 -0.47033471665558935 2207 14 -5.548537992344688 2207 15 -2.134397554894708 2207 16 -1.3394026209554752 2207 17 -0.1776769075065303 2207 18 -0.22127099587305565 2207 19 -0.9480444384878446 2207 20 -1.1279781133425217 2207 21 -3.905385386913352 2207 22 -4.565949680469306 2207 23 -1.8202054852372278 2207 24 -0.3604257765419163 2207 25 0.03863025514408136 2208 1 4.390457934946596 2208 2 5.784450376387628 2208 3 6.005716108479 2208 4 7.948285876554721 2208 5 3.2808011423201293 2208 6 5.7488905859054995 2208 7 6.018202674741424 2208 8 6.0265410927483565 2208 9 4.550473980624954 2208 10 4.228236644763168 2208 11 6.170505245985293 2208 12 5.887791338787455 2208 13 7.949933434311092 2208 14 2.5143605062155743 2208 15 4.2319897342866994 2208 16 4.637053827008327 2208 17 5.496428398951718 2208 18 0.4765771119015234 2208 19 3.0210526409033527 2208 20 3.9773219175089807 2208 21 6.600116464176736 2208 22 5.675579011427033 2208 23 1.7708257261983924 2208 24 3.004232543079638 2208 25 4.147542482622484 2209 1 2.55022800947901 2209 2 4.0429982727326 2209 3 4.423826334633102 2209 4 -0.3051099506528061 2209 5 4.181855526692612 2209 6 4.8621082640226305 2209 7 5.1608322115665715 2209 8 3.6339099578868748 2209 9 3.4508460034832673 2209 10 2.606432717429076 2209 11 -1.1953332117178839 2209 12 2.2075376345392357 2209 13 1.5550426760748124 2209 14 3.02135191517225 2209 15 3.637815855149384 2209 16 3.091068733491241 2209 17 1.2155239743087165 2209 18 2.9621324614879505 2209 19 3.5555446571138347 2209 20 3.7712902891738764 2209 21 5.66463558308342 2209 22 4.487554056491657 2209 23 0.11337440238509444 2209 24 -3.1367542123952354 2209 25 -0.39677106489033065 2210 1 -2.309296258228649 2210 2 -12.812577595640345 2210 3 -7.309264963081018 2210 4 -2.486811920385179 2210 5 -1.079719915569349 2210 6 -1.8500039406206064 2210 7 -1.8640729443763 2210 8 -19.242689676482414 2210 9 -12.484477909590094 2210 10 -7.44413703418946 2210 11 -9.064798923529404 2210 12 -4.903483176171271 2210 13 -5.5173755724716 2210 14 -4.465679190929386 2210 15 -6.006771504474539 2210 16 -5.466040447138578 2210 17 -4.903848955273006 2210 18 -5.361847621260054 2210 19 -9.996640192578953 2210 20 -7.399781242488917 2210 21 -5.480960353742187 2210 22 -9.428856027218533 2210 23 -7.9088761508719045 2210 24 -10.726766720814362 2210 25 -8.678177849676796 2211 1 -0.27565612340884016 2211 2 4.026192208646702 2211 3 -0.005816161673958931 2211 4 0.8024095022300699 2211 5 -0.028695628383538008 2211 6 -1.135917057522832 2211 7 -19.122531590992843 2211 8 -12.619204121962376 2211 9 -9.272853204771197 2211 10 -7.792881682389641 2211 11 -9.213020128919394 2211 12 -5.554986410024775 2211 13 -6.552727232142679 2211 14 -5.4354428358291464 2211 15 -5.735519340980996 2211 16 -11.172430723855534 2211 17 -7.9921264990859395 2211 18 -6.341129761839834 2211 19 -9.017304621591839 2211 20 -6.951835597855432 2211 21 -4.795420198601306 2211 22 -6.310900957811558 2211 23 -8.084735767294134 2211 24 -19.910977247130152 2211 25 -19.682877097797096 2212 1 -1.730828537201944 2212 2 -12.812577595640345 2212 3 -7.737116920560924 2212 4 -18.22741342414458 2212 5 -18.7742687877498 2212 6 -19.048312211297297 2212 7 -9.667359177199739 2212 8 -9.192443371063694 2212 9 -5.631340756362196 2212 10 -5.981964361127492 2212 11 -6.024949299469254 2212 12 -3.484316725561989 2212 13 -6.056654805035768 2212 14 -4.2790056104264025 2212 15 -4.301968548809512 2212 16 -4.347826809596448 2212 17 -4.604082297248635 2212 18 -3.5616596513352405 2212 19 -3.957179471811518 2212 20 -4.263671571760373 2212 21 -2.184369739274988 2212 22 -3.4193019696608467 2212 23 -3.2764545797026017 2212 24 -2.428218626440497 2212 25 -2.989281038683796 2213 1 -16.965311295822634 2213 2 -12.812577595640345 2213 3 -8.614344740771637 2213 4 -6.36503828540984 2213 5 -18.7742687877498 2213 6 -10.512241179110838 2213 7 -19.122531590992843 2213 8 -10.491302329090626 2213 9 -8.603228587617014 2213 10 -7.860700084434381 2213 11 -7.086658511455935 2213 12 -5.711565048443514 2213 13 -17.50985677547912 2213 14 -11.826180889126041 2213 15 -7.5509486092787235 2213 16 -6.946870018291765 2213 17 -5.565995442467685 2213 18 -5.510636978115009 2213 19 -6.42404332860993 2213 20 -6.1589679857065605 2213 21 -4.540929227090931 2213 22 -5.155607005663537 2213 23 -4.95281855404966 2213 24 -4.134062707729488 2213 25 -5.103520826986884 2214 1 -16.965311295822634 2214 2 -12.812577595640345 2214 3 -11.856767849510575 2214 4 -18.22741342414458 2214 5 -18.7742687877498 2214 6 -19.048312211297297 2214 7 -15.543255685874241 2214 8 -14.246434994834473 2214 9 -13.48132630398432 2214 10 -13.475432501321709 2214 11 -13.769768275748905 2214 12 -12.027833142848262 2214 13 -17.50985677547912 2214 14 -15.642356840108512 2214 15 -21.667485790188824 2214 16 -17.01179856903508 2214 17 -21.317382789859472 2214 18 -16.481081802218064 2214 19 -14.082022186890574 2214 20 -12.963495068251827 2214 21 -10.748091195828678 2214 22 -11.703069181827903 2214 23 -11.61182969578287 2214 24 -10.67002843314763 2214 25 -10.382003190181615 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 137561.0 3 2.0 4 745.0 5 2735.0 6 3641.0 7 3311.0 8 2857.0 9 2793.0 10 2514.0 11 2410.0 12 2262.0 13 2391.0 14 3376.0 15 6106.0 16 8415.0 17 9298.0 18 9782.0 19 11423.0 20 13960.0 21 16084.0 22 17251.0 23 18423.0 24 19220.0 25 19329.0 26 16362.0 27 13884.0 28 15172.0 29 17181.0 30 16647.0 31 19458.0 32 23906.0 33 28866.0 34 33916.0 35 36390.0 36 34728.0 37 33423.0 38 18545.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.11074407314803 17.534721588524082 12.713120289088023 25.64141404923987 2 13.956446147919117 20.0998212010155 41.08795724629807 24.855775404767314 3 20.176376044497925 24.20525593880694 27.74893341299427 27.869434603700867 4 11.767838612917334 16.47984217066578 36.60216722515753 35.15015199125936 5 12.597428796742898 37.46474223779045 36.51768848460396 13.420140480862692 6 32.54540613107588 35.44729338407429 17.642098722969898 14.36520176187993 7 27.71507416714949 30.66133182116762 21.969431476175465 19.654162535507425 8 25.429347385068535 37.01477745760049 20.492305673583765 17.063569483747216 9 26.561375097207634 15.537989144813322 19.1076940344576 38.79294172352144 10 14.852161304469549 29.66774930130418 33.66346407481669 21.81662531940958 11 33.64520435182956 22.211299067922006 23.678518102042087 20.464978478206344 12 24.671485495616167 24.752322163532074 30.67390219267361 19.90229014817815 13 29.10071375498165 20.608455482253696 24.351353582659733 25.939477180104927 14 20.574784544927123 22.827217834072854 26.85142270032703 29.74657492067299 15 24.440232063875005 30.070817229183593 22.754732773688485 22.734217933252914 16 21.34810065284159 27.81807765482622 28.822866035329287 22.010955657002903 17 21.943629718575096 27.880848802188936 28.973346981358635 21.202174497877337 18 21.991761044378304 26.549419160105835 30.838760658986907 20.62005913652895 19 23.3183094488635 26.719155857291977 27.050436555781225 22.912098138063293 20 23.861343835933283 27.489655881370307 27.20005268433091 21.4489475983655 21 21.76089017514096 25.923689769306257 26.807211882307936 25.50820817324485 22 22.339009701970383 28.084813214780713 26.800943449751575 22.77523363349733 23 21.49069358749054 27.739811691060456 27.88348063676129 22.886014084687712 24 22.744916092547687 28.06579534810431 27.28476009559409 21.904528463753905 25 22.642117568202483 26.733544893452915 27.19873128069773 23.425606257646873 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 670.0 1 670.0 2 1199.5 3 1729.0 4 1729.0 5 1729.0 6 2361.0 7 2993.0 8 2993.0 9 2993.0 10 3626.0 11 4259.0 12 4259.0 13 4259.0 14 5177.0 15 6095.0 16 6095.0 17 6095.0 18 8657.0 19 11219.0 20 11219.0 21 11219.0 22 15907.5 23 20596.0 24 20596.0 25 20596.0 26 27578.5 27 34561.0 28 34561.0 29 34561.0 30 42584.0 31 50607.0 32 50607.0 33 50607.0 34 56382.5 35 62158.0 36 62158.0 37 62158.0 38 66141.5 39 70125.0 40 70125.0 41 70125.0 42 73588.5 43 77052.0 44 77052.0 45 77052.0 46 80955.0 47 84858.0 48 84858.0 49 84858.0 50 77849.0 51 70840.0 52 70840.0 53 70840.0 54 60592.5 55 50345.0 56 50345.0 57 50345.0 58 42365.5 59 34386.0 60 34386.0 61 34386.0 62 27916.5 63 21447.0 64 21447.0 65 21447.0 66 16533.0 67 11619.0 68 11619.0 69 11619.0 70 8519.0 71 5419.0 72 5419.0 73 5419.0 74 3831.0 75 2243.0 76 2243.0 77 2243.0 78 1531.0 79 819.0 80 819.0 81 819.0 82 521.0 83 223.0 84 223.0 85 223.0 86 151.5 87 80.0 88 80.0 89 80.0 90 49.0 91 18.0 92 18.0 93 18.0 94 11.5 95 5.0 96 5.0 97 5.0 98 3.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.24231902070417 2 35.146476351248545 3 4.834015891294703 4 12.485285096745985 5 12.668350505391862 6 13.386517865293971 7 9.053649536250315 8 7.235167777925483 9 0.11243387302660134 10 1.029843025015736 11 4.855797952165954 12 4.6991593085476975 13 14.640587987513754 14 1.1693443119191116 15 2.410761619368096 16 2.431903031390192 17 2.36319344231838 18 1.091185152322272 19 1.0349682158089715 20 0.2868505222088932 21 3.705673105721475 22 1.1983336723433493 23 0.1138753329371988 24 8.52095001817841 25 0.1194810103673 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 624367.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.82848122381229 #Duplication Level Percentage of deduplicated Percentage of total 1 96.78110233793376 86.93699434182938 2 2.219791920041943 3.9880107362051573 3 0.4393459212475735 1.183973305126385 4 0.18470336013778 0.6636648927244643 5 0.08585130104198302 0.3855945991844813 6 0.05824724131964467 0.31393567339323386 7 0.039546422516158565 0.24866765507231878 8 0.02674167018808124 0.1921730894706749 9 0.0217383697013735 0.17574522611005075 >10 0.11838988681799882 1.8998418937890411 >50 0.013014527506156911 0.8003139541074643 >100 0.010039681347983973 1.8738424914300864 >500 0.0011155201496884943 0.6790591416974997 >1k 3.7184004989616475E-4 0.6581829998597754 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 1991 0.31888296466661437 No Hit CCCATGTACTCTGCGTTGATACCAC 1950 0.3123163139627815 No Hit GAGTACATGGGAAGCAGTGGTATCA 922 0.14766955973009463 No Hit CATGTACTCTGCGTTGATACCACTG 731 0.11707857718297092 No Hit GCGTTGATACCACTGCTTCCCATGT 683 0.10939079099311783 No Hit GTCCTACAGTGGACATTTCTAAATT 660 0.1057070601104799 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTGT 50 1.1748485E-4 18.583805 1 CCAACGA 50 6.3384687E-7 17.152931 19 CCTAGGC 45 2.4014505E-4 16.927326 3 GTCCTAC 520 0.0 16.33741 1 TGTAGGA 410 0.0 16.23436 2 AAATAGC 40 0.0029485421 15.551208 6 TCCTAGA 70 7.2515795E-5 15.524403 2 CGCAGGG 45 5.686576E-4 15.115764 8 TCCTACA 595 0.0 14.839502 2 GTATTGT 55 0.004735647 14.480887 1 TAGGACA 155 0.0 14.469436 4 CTGTAGG 410 0.0 14.245426 1 TAAACCT 45 0.0052965814 14.239762 4 TCCAACG 80 2.9307557E-8 13.945473 18 CCTACAG 705 0.0 13.274318 3 CCTGTAC 110 8.452116E-8 12.860372 3 CCTAGAC 60 0.0021237843 12.695497 3 TCAGCAC 60 0.0021237843 12.695497 3 GTAGGAC 450 0.0 12.574586 3 TAGGACC 155 4.0017767E-11 12.402373 4 >>END_MODULE