FastQCFastQC Report
Thu 26 May 2016
SRR1926537_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926537_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences624367
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC59360.9507228921451646No Hit
GTACATGGGAAGCAGTGGTATCAAC53250.8528637804368263No Hit
GAGTACATGGGAAGCAGTGGTATCA24180.3872722293138491No Hit
CATGTACTCTGCGTTGATACCACTG20190.32336750661069535No Hit
GCGTTGATACCACTGCTTCCCATGT16860.27003348991858955No Hit
ACGCAGAGTACATGGGAAGCAGTGG14320.22935228799728363No Hit
GTATCAACGCAGAGTACATGGGAAG14170.22694985481295457No Hit
GTCCTACAGTGGACATTTCTAAATT13780.22070352853369893No Hit
CTGTAGGACGTGGAATATGGCAAGA13130.21029298473493954No Hit
ACTCTGCGTTGATACCACTGCTTCC11030.17665892015433232No Hit
GTATCAACGCAGAGTACTTTTTTTT10910.17473697360686902No Hit
CTTTAGGACGTGAAATATGGCGAGG9830.15743945467969958No Hit
GATATACACTGTTCTACAAATCCCG9790.15679880583054517No Hit
GTCCTACAGTGTGCATTTCTCATTT9710.15551750813223633No Hit
TATCAACGCAGAGTACATGGGAAGC9500.15215410167417562No Hit
GTCCTAAAGTGTGTATTTCTCATTT9200.14734923530551744No Hit
GCTTCCCATGTACTCTGCGTTGATA8540.13677852929446943No Hit
GGTATCAACGCAGAGTACATGGGAA8490.1359777182330264No Hit
CTGTAGGACCTGGAATATGGCGAGA7800.1249265255851126No Hit
CAGTGGTATCAACGCAGAGTACATG7350.11771922603212534No Hit
GGTATCAACGCAGAGTACTTTTTTT6970.1116330619651583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2800.014.3064184
TCCAACG1203.6379788E-1214.21131718
GTAGGAC7800.014.1864283
TGTAGGA7950.014.1587382
TCCTATA551.8900588E-413.8751282
CTATAGT903.44171E-813.776554
GATATAC3700.013.6586811
GACGTGG3900.013.3964747
CTGTAGG8000.013.2302371
TAGGACG6550.013.1051164
AGGACGT6350.013.0672795
GGACGTG6100.012.976366
TCTAAAG603.946534E-412.7188662
ACTGTTC3750.012.6657568
ATACACT4250.012.34279254
CACTGTT4300.012.15029057
GTCTAGG550.002980889812.1358431
ACGTGGA4350.012.0106328
TATACAC4600.011.82025053
CCTACCC657.7397795E-411.7404923
Produced by FastQC (version 0.11.5)