FastQCFastQC Report
Thu 26 May 2016
SRR1926536_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926536_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences551525
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC16180.2933683876524183No Hit
GTACATGGGAAGCAGTGGTATCAAC15010.27215448075789855No Hit
GTCCTACAGTGGACATTTCTAAATT7950.1441457776166085No Hit
GAGTACATGGGAAGCAGTGGTATCA7130.1292779112460904No Hit
CATGTACTCTGCGTTGATACCACTG5960.10806400435157064No Hit
CTGTAGGACGTGGAATATGGCAAGA5770.10461901092425546No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTCG454.1945554E-521.2460862
GCTACAC358.409328E-418.75563
GCCTCGG552.9337662E-617.9030743
GTAGGGC502.5934474E-517.5052283
CTCGGTC453.3535823E-416.201525
CCCTTAG604.5828478E-415.548381
TGTAGGA5050.015.4161842
CGGTCTC400.00332164915.2749647
GTCCTAG708.5291016E-515.2310651
TAGGACC2700.015.0996584
CTGTAGG5050.015.04256251
TTCAAGG550.0039162814.8998522
CCCTACA656.9838326E-414.7088292
GCCCACA550.00461016514.5387441
TGTACTG807.059243E-714.3209875
TTATGTA450.00513695514.304944
CTACACT754.1936437E-614.3049394
TATACAC2700.013.7772633
GTCCTAC5650.013.6810021
TCCTACA6000.013.6581972
Produced by FastQC (version 0.11.5)