##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926536_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 551525 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 18.587714065545534 26.0 2.0 30.0 2.0 31.0 2 14.595853315806174 10.0 2.0 28.0 2.0 31.0 3 17.604157563120438 16.0 2.0 30.0 2.0 31.0 4 19.450989529033137 25.0 2.0 32.0 2.0 35.0 5 19.495654775395494 25.0 2.0 33.0 2.0 35.0 6 20.433059244821177 27.0 2.0 35.0 2.0 35.0 7 20.019154163455873 26.0 2.0 33.0 2.0 35.0 8 20.266927156520556 26.0 2.0 35.0 2.0 35.0 9 22.750076605774897 29.0 2.0 35.0 2.0 39.0 10 23.340624631703005 30.0 2.0 35.0 2.0 39.0 11 20.822341688953358 27.0 2.0 35.0 2.0 39.0 12 21.831532568786546 28.0 2.0 35.0 2.0 39.0 13 18.89079370835411 19.0 2.0 34.0 2.0 38.0 14 21.717205929015005 27.0 2.0 36.0 2.0 39.0 15 22.68512034812565 29.0 2.0 36.0 2.0 40.0 16 22.988062191197134 30.0 2.0 37.0 2.0 40.0 17 22.307364126739493 29.0 2.0 36.0 2.0 40.0 18 22.28414124473052 30.0 2.0 36.0 2.0 40.0 19 22.619455147092154 30.0 2.0 36.0 2.0 40.0 20 22.89299487783872 30.0 2.0 37.0 2.0 40.0 21 20.90417660124201 27.0 2.0 35.0 2.0 40.0 22 21.69932641312724 30.0 2.0 36.0 2.0 40.0 23 21.61998096187843 30.0 2.0 36.0 2.0 40.0 24 20.9173745523775 29.0 2.0 37.0 2.0 40.0 25 20.673615883232856 29.0 2.0 36.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 4.663870818324131 1101 2 8.689679081364716 1101 3 5.642847111364869 1101 4 7.067578815057981 1101 5 6.747710646489459 1101 6 6.505217347474119 1101 7 5.793550253137827 1101 8 4.54100252273329 1101 9 4.75612607490006 1101 10 4.795841731551047 1101 11 7.063725586566516 1101 12 4.794106025057626 1101 13 8.842880972934488 1101 14 6.536196233962379 1101 15 5.906667387141695 1101 16 5.318891156824087 1101 17 1.8788540354036911 1101 18 4.041696788271622 1101 19 4.304321315695738 1101 20 2.460897581180035 1101 21 -0.03194165818127814 1101 22 3.412026454206945 1101 23 4.187545472016517 1101 24 5.2965103585110604 1101 25 5.714503647543673 1102 1 5.0158972145900265 1102 2 9.175223370221413 1102 3 5.818330774651528 1102 4 7.155132868387021 1102 5 6.807523941194109 1102 6 5.721255761552129 1102 7 5.882994965837007 1102 8 5.776309137895048 1102 9 5.230318896403357 1102 10 4.867466818733835 1102 11 7.210268856055222 1102 12 4.566637813243691 1102 13 8.583303742396371 1102 14 6.48172871961075 1102 15 4.779976041039436 1102 16 4.997677572404321 1102 17 5.747145252743678 1102 18 6.126447043113622 1102 19 5.058503407009649 1102 20 5.639431837309655 1102 21 3.760743571713032 1102 22 5.1728709201117695 1102 23 5.723331726643909 1102 24 6.836038230177994 1102 25 6.38566948262066 1103 1 4.058392424778237 1103 2 7.470453844694536 1103 3 4.9853678534298425 1103 4 6.519616132221124 1103 5 5.911545129067434 1103 6 5.586557237941172 1103 7 5.447784777625579 1103 8 4.8075094083785395 1103 9 4.388567515810546 1103 10 3.6495427795137303 1103 11 4.579203827316352 1103 12 3.556264771431451 1103 13 6.9923897336056555 1103 14 5.6333166830954795 1103 15 4.770148474263827 1103 16 4.6471985912254645 1103 17 2.430391054614521 1103 18 1.2442628889380316 1103 19 3.1029754006983694 1103 20 3.5287139290486387 1103 21 5.469971566754168 1103 22 1.5678151328921146 1103 23 3.7664242545375224 1103 24 4.872179332398211 1103 25 1.3808210660760878 1104 1 5.526918886416649 1104 2 9.398897260830289 1104 3 6.62881581077124 1104 4 7.166154540213647 1104 5 7.029340246663665 1104 6 6.618531303347794 1104 7 6.509042437457232 1104 8 7.119467032631892 1104 9 5.898966987218632 1104 10 5.50893224701041 1104 11 7.991672364827149 1104 12 4.897267328207569 1104 13 8.82939249368636 1104 14 7.0819557577944465 1104 15 6.732917217510025 1104 16 6.24236281492238 1104 17 6.438909958626031 1104 18 4.380461490998037 1104 19 5.164592158299641 1104 20 4.06595402513215 1104 21 2.782353478833773 1104 22 5.041828608450263 1104 23 5.469544316943189 1104 24 6.063633689414321 1104 25 5.54659827519032 1105 1 2.887958931702009 1105 2 8.118046874334095 1105 3 5.614655703418798 1105 4 5.082648593348047 1105 5 2.156443891734405 1105 6 4.082813742305852 1105 7 4.75921452392528 1105 8 2.7447022350403643 1105 9 3.476148690500448 1105 10 3.3281850596886215 1105 11 6.347208805520303 1105 12 3.564273424232347 1105 13 4.268984082163048 1105 14 3.504844681118197 1105 15 4.190738903251145 1105 16 4.194758374511736 1105 17 4.7381822937629785 1105 18 2.279399034942017 1105 19 2.1735079766564454 1105 20 1.4749084245899091 1105 21 2.3866916390212403 1105 22 3.5695474428793617 1105 23 1.2454713965541302 1105 24 4.2325577721381045 1105 25 4.736686094630766 1106 1 6.456677143687379 1106 2 10.542504526479934 1106 3 7.690727829464727 1106 4 9.108020787667087 1106 5 8.411129068000765 1106 6 7.445875341590305 1106 7 7.272392293352688 1106 8 6.96626277847318 1106 9 6.421636049389448 1106 10 5.955751520124618 1106 11 7.983145867236022 1106 12 5.401309206753371 1106 13 4.218258746486907 1106 14 5.6620559625886635 1106 15 5.757287194570935 1106 16 5.93629224379783 1106 17 5.978604211020198 1106 18 6.023752742595487 1106 19 6.2156891173996165 1106 20 5.745594913393646 1106 21 5.923225164295523 1106 22 6.351616488696443 1106 23 6.748867587020239 1106 24 7.343270299989829 1106 25 6.574330513436042 1107 1 3.096975572399007 1107 2 -11.996355577147007 1107 3 -4.687804931211909 1107 4 1.424119898079379 1107 5 3.834701559373979 1107 6 4.193370672046285 1107 7 4.008221678239192 1107 8 4.471541209928493 1107 9 3.211369384129803 1107 10 2.6618477813236083 1107 11 4.975879318055437 1107 12 2.6393229885036327 1107 13 3.659502691908642 1107 14 3.3064066057721853 1107 15 3.2406256283478285 1107 16 3.001440033546995 1107 17 3.4902173933804015 1107 18 -1.1322902277621445 1107 19 1.7060862133125774 1107 20 2.049636575223687 1107 21 -0.8368510257786603 1107 22 1.7087611548636197 1107 23 -0.8973371553370093 1107 24 2.0137250133273135 1107 25 2.773711073359216 1108 1 5.117462937943635 1108 2 9.730277588682139 1108 3 0.5445513512185727 1108 4 5.610017819458168 1108 5 6.979822274968242 1108 6 7.289872529602906 1108 7 7.556668597151475 1108 8 7.123963197667596 1108 9 5.853296944538773 1108 10 4.981621804187419 1108 11 6.794669808184288 1108 12 2.485606340088438 1108 13 3.63375071409698 1108 14 5.033535120033921 1108 15 4.859690402319522 1108 16 5.026109204247749 1108 17 -0.027655064632678972 1108 18 3.6055425205005562 1108 19 4.879661830707086 1108 20 5.269926415463583 1108 21 4.929973934051038 1108 22 2.123090438757629 1108 23 4.686071742512727 1108 24 5.113864202143084 1108 25 5.771773687857369 1109 1 2.9494577234008545 1109 2 -11.996355577147007 1109 3 -4.594044064886342 1109 4 1.9725187943993916 1109 5 0.26591348489816014 1109 6 2.0955071327739745 1109 7 2.9301579953620376 1109 8 3.505159645538882 1109 9 3.155388332743655 1109 10 2.554137375391555 1109 11 0.6268495539196159 1109 12 1.4002683418934723 1109 13 2.0846994711861946 1109 14 3.2597214842273914 1109 15 3.320400828062425 1109 16 3.1772317794296434 1109 17 3.614611701570844 1109 18 3.803963672825372 1109 19 2.2842356978253804 1109 20 1.2988203307161186 1109 21 0.7044872991024569 1109 22 2.90971535936956 1109 23 3.308415166951999 1109 24 4.24209961423681 1109 25 4.524710210826985 1110 1 3.3162267148111653 1110 2 8.473182237979042 1110 3 6.265020292563957 1110 4 7.596384526945183 1110 5 8.31564424194599 1110 6 4.193000564736561 1110 7 6.296267853939614 1110 8 7.211295869429769 1110 9 6.067660780525429 1110 10 5.942774204846174 1110 11 3.6841941103674714 1110 12 6.32447505340291 1110 13 10.101543459335776 1110 14 8.191604880601464 1110 15 7.736013192742224 1110 16 7.367602384434395 1110 17 7.601116946684414 1110 18 7.651783177567403 1110 19 6.4834591787920495 1110 20 6.973388051374226 1110 21 4.787605579674111 1110 22 6.834914263765022 1110 23 7.145830620938472 1110 24 7.842898026728193 1110 25 8.264809243788285 1111 1 4.923508550622415 1111 2 8.52261878182735 1111 3 6.948695147121164 1111 4 7.75810182925072 1111 5 7.8866332529350025 1111 6 7.295371820931344 1111 7 7.223453805243214 1111 8 7.481788220216238 1111 9 5.9722352256881095 1111 10 5.469167223592134 1111 11 7.056125806122697 1111 12 6.534600026469061 1111 13 7.7951525952978535 1111 14 6.270473658882008 1111 15 5.847506405411906 1111 16 5.895734252566264 1111 17 7.080748491610073 1111 18 6.731062427729004 1111 19 5.759502057481985 1111 20 5.478487167943932 1111 21 7.703982438109794 1111 22 7.133707627264265 1111 23 7.065625923674951 1111 24 6.6648911316624755 1111 25 7.092093234307569 1112 1 -1.1167668529863235 1112 2 7.118870760301554 1112 3 5.649331424424108 1112 4 0.6271363338407809 1112 5 3.759206852175268 1112 6 2.5588191143786396 1112 7 5.3755810607038015 1112 8 5.514149727022186 1112 9 4.45320388665677 1112 10 3.2662517440099883 1112 11 -0.762009679790566 1112 12 3.4370818245064143 1112 13 7.090511886212703 1112 14 5.1080899931650094 1112 15 3.1170347371624594 1112 16 3.3884724608600294 1112 17 4.5267662187389135 1112 18 4.591796837352305 1112 19 4.181072174607657 1112 20 3.5449765633222157 1112 21 -0.6875085400478511 1112 22 2.2159963262196314 1112 23 3.387332159825629 1112 24 4.683605914821701 1112 25 4.877800736681738 1113 1 -16.027260680146817 1113 2 -11.996355577147007 1113 3 -6.514248822489472 1113 4 -5.36575554793999 1113 5 -1.4029704766687274 1113 6 -0.8292280236684988 1113 7 -17.399110297320888 1113 8 -7.979770961342945 1113 9 -4.354315754196204 1113 10 -3.5494048216465757 1113 11 -4.8084930318294745 1113 12 -2.7595137609780593 1113 13 -1.9989007587555 1113 14 -2.125074989999977 1113 15 -2.3382589403534375 1113 16 -2.286798769966758 1113 17 -2.1192307883692045 1113 18 -2.547559690893113 1113 19 -2.2074426027960072 1113 20 -2.828386698929936 1113 21 -0.4619193144586262 1113 22 -1.3516243247343525 1113 23 -1.4881822434665501 1113 24 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2.3530125051557995 2204 4 4.449561085526728 2204 5 0.39791798914120236 2204 6 2.7503161087552144 2204 7 4.196701221003718 2204 8 3.7090509400444027 2204 9 2.674165686152598 2204 10 2.4399606186787253 2204 11 3.599737029105647 2204 12 4.231719102855152 2204 13 2.1444665971773027 2204 14 3.4963939166347267 2204 15 3.575126220152139 2204 16 3.3735111353693235 2204 17 -1.2303912586919807 2204 18 2.3414208651031494 2204 19 2.775670670718661 2204 20 3.266464077596236 2204 21 4.5711431308202215 2204 22 4.048434436231911 2204 23 3.0369938925298285 2204 24 4.158369450899954 2204 25 4.210122499980816 2205 1 5.096082846866434 2205 2 8.525560834677663 2205 3 6.270777794341424 2205 4 6.579301546964199 2205 5 6.900615845266216 2205 6 6.27382081332529 2205 7 6.450023239886043 2205 8 6.135294061978961 2205 9 5.088440040241547 2205 10 4.728563444089275 2205 11 6.764031396964068 2205 12 6.00001835941033 2205 13 8.65327852645131 2205 14 6.492363410313043 2205 15 5.3439762556432235 2205 16 5.070165366813892 2205 17 5.794246492080553 2205 18 5.732828286742571 2205 19 4.2227718763481334 2205 20 4.870683513901746 2205 21 -0.9198111790783194 2205 22 3.5353699812201995 2205 23 4.755333443474214 2205 24 6.456551133230732 2205 25 6.921435349888217 2206 1 4.006275905219038 2206 2 8.0077094635034 2206 3 5.697622601780324 2206 4 1.5516946997948082 2206 5 4.947177342991285 2206 6 5.60918045132534 2206 7 6.010442548207568 2206 8 5.749708795575835 2206 9 4.771519153142766 2206 10 4.180246022841665 2206 11 3.3210476322640368 2206 12 4.674196649358368 2206 13 7.441222013684346 2206 14 0.21306590403522918 2206 15 2.3374000889641273 2206 16 3.388798667825803 2206 17 4.328118723004696 2206 18 -0.709894420301012 2206 19 1.331353214455092 2206 20 2.4552320084692596 2206 21 3.3936040766338706 2206 22 -1.8266113220790743 2206 23 -2.153983207031743 2206 24 1.216138786446713 2206 25 2.4842445788979397 2207 1 0.9066236173738424 2207 2 -11.996355577147007 2207 3 -5.387004153578138 2207 4 -4.0353173273502545 2207 5 0.5147292478491785 2207 6 1.2407903418044128 2207 7 2.4335343307782864 2207 8 2.4136710343717773 2207 9 0.518898713715231 2207 10 -0.07030830088339002 2207 11 -2.0360208351148543 2207 12 -2.5540755403883253 2207 13 -0.1261199200655696 2207 14 -5.705028566165673 2207 15 -2.0017116492606952 2207 16 -1.2672854549988983 2207 17 -0.06191520832635078 2207 18 0.0252900183202307 2207 19 -0.6036649270486372 2207 20 -0.6876995241518493 2207 21 -3.3558332007967557 2207 22 -4.365985104246647 2207 23 -1.79942599410424 2207 24 -0.41935976895206295 2207 25 0.12582389758368606 2208 1 4.598731383345246 2208 2 5.5899539466625185 2208 3 5.7490889200149375 2208 4 7.321874723023612 2208 5 3.7799299562317046 2208 6 6.072377315436839 2208 7 6.295334147123555 2208 8 6.008564776992792 2208 9 5.0282700242229055 2208 10 4.656242925667836 2208 11 6.383015305512195 2208 12 6.152322859191894 2208 13 8.062035989681249 2208 14 3.061352779761126 2208 15 4.638382473787974 2208 16 4.952919844751857 2208 17 5.89044934631093 2208 18 1.5495422812088577 2208 19 3.503125378605308 2208 20 4.547034176490939 2208 21 6.911146662774016 2208 22 6.088240594297233 2208 23 2.15224985529888 2208 24 3.2491188483901894 2208 25 4.343298973013308 2209 1 2.7068255621529786 2209 2 4.1514883653581265 2209 3 4.641719945731179 2209 4 0.5677190566502865 2209 5 4.435620774653529 2209 6 4.908332385349816 2209 7 5.524914343336196 2209 8 4.266338317620544 2209 9 3.773228223108209 2209 10 3.179901704716759 2209 11 -0.8809815492187454 2209 12 2.3638992419695093 2209 13 1.682246787895128 2209 14 2.866354246466905 2209 15 3.5179312987936804 2209 16 3.024393232190338 2209 17 0.5764806034407499 2209 18 2.9030794866454492 2209 19 3.378518537237685 2209 20 3.720659547241567 2209 21 5.663075788291437 2209 22 4.637124675650515 2209 23 0.660320811265116 2209 24 -2.3216965585391804 2209 25 0.21553871422122128 2210 1 -2.9945602582058886 2210 2 -11.996355577147007 2210 3 -7.130243509159266 2210 4 -2.941989050333831 2210 5 -1.6658204958478606 2210 6 -2.348471087215362 2210 7 -2.2872959513293267 2210 8 -17.670006651578635 2210 9 -11.735028649677034 2210 10 -7.652251147066741 2210 11 -9.529369163043159 2210 12 -5.320979330882448 2210 13 -5.109063568285398 2210 14 -4.3703832057723275 2210 15 -5.926930465733825 2210 16 -5.472964221954996 2210 17 -5.103045731044908 2210 18 -5.3810635054095854 2210 19 -9.419933190814023 2210 20 -7.225469339683462 2210 21 -5.780999710543243 2210 22 -9.385425770983057 2210 23 -8.099684040622357 2210 24 -9.966209160450475 2210 25 -8.269331627750203 2211 1 -0.7357279899003011 2211 2 3.216934883731877 2211 3 -0.22358946001875069 2211 4 0.4867422777625521 2211 5 -0.06729864582004907 2211 6 -1.0347559719617223 2211 7 -17.399110297320888 2211 8 -11.87900986701272 2211 9 -8.884118966743255 2211 10 -6.994148795865364 2211 11 -8.396379759051678 2211 12 -4.713018078898241 2211 13 -6.339628507843377 2211 14 -4.753874597363357 2211 15 -5.214935675551075 2211 16 -10.555303755513544 2211 17 -7.213561014532228 2211 18 -5.407336450991416 2211 19 -7.778840757259543 2211 20 -6.205556034298628 2211 21 -4.137828098620357 2211 22 -5.576062345528214 2211 23 -7.274375725265605 2211 24 -18.39869861192727 2211 25 -18.143804201589866 2212 1 -0.6358133117257658 2212 2 -11.996355577147007 2212 3 -6.570616593769525 2212 4 -16.809077657928768 2212 5 -16.827212900911153 2212 6 -17.7282179226584 2212 7 -8.134855911355976 2212 8 -7.88162945859618 2212 9 -4.105031980789626 2212 10 -4.373936690038093 2212 11 -4.448020617628989 2212 12 -2.021914818335258 2212 13 -4.95608222251591 2212 14 -2.8387098768804755 2212 15 -2.890857292293898 2212 16 -2.728418918823749 2212 17 -2.8719775542738653 2212 18 -1.8552196235530474 2212 19 -2.3632071193061392 2212 20 -2.4796471100587674 2212 21 -0.5516248493395999 2212 22 -1.822786974625071 2212 23 -1.912808624233282 2212 24 -1.0346635242079714 2212 25 -1.6087164822916193 2213 1 -16.027260680146817 2213 2 -11.996355577147007 2213 3 -7.824615561777781 2213 4 -5.600744324595434 2213 5 -16.827212900911153 2213 6 -9.48557086383487 2213 7 -17.399110297320888 2213 8 -9.254565475108047 2213 9 -7.609159947765889 2213 10 -6.879354646694441 2213 11 -6.091015973666142 2213 12 -4.961414302339385 2213 13 -16.234590994445735 2213 14 -10.6343755115554 2213 15 -6.526241709218562 2213 16 -5.872768764024986 2213 17 -4.469436274604103 2213 18 -4.411592172572654 2213 19 -5.124559028490864 2213 20 -5.051822032659388 2213 21 -3.6098033839113235 2213 22 -4.234938677411449 2213 23 -4.063027922478895 2213 24 -3.3570319452606014 2213 25 -3.8913532211977078 2214 1 -16.027260680146817 2214 2 -11.996355577147007 2214 3 -11.426009067583053 2214 4 -16.809077657928768 2214 5 -16.827212900911153 2214 6 -17.7282179226584 2214 7 -14.32048621869681 2214 8 -13.511529993101977 2214 9 -13.381676448223566 2214 10 -13.386954492529581 2214 11 -13.173054563057672 2214 12 -11.965735133866099 2214 13 -16.234590994445735 2214 14 -15.052108083699736 2214 15 -20.146339748434247 2214 16 -16.33703082019881 2214 17 -19.762328431466848 2214 18 -15.806181949515373 2214 19 -14.028642590662663 2214 20 -13.108712479255715 2214 21 -10.908714594587241 2214 22 -11.760836567279929 2214 23 -11.677022598973572 2214 24 -10.8311310443597 2214 25 -10.546752604538268 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 148426.0 3 6.0 4 696.0 5 2543.0 6 3042.0 7 2829.0 8 2538.0 9 2414.0 10 2301.0 11 2179.0 12 2070.0 13 2188.0 14 3003.0 15 5391.0 16 7507.0 17 7982.0 18 8922.0 19 10529.0 20 12381.0 21 14400.0 22 15378.0 23 16369.0 24 16492.0 25 16369.0 26 14205.0 27 12641.0 28 13483.0 29 15011.0 30 14683.0 31 17069.0 32 20494.0 33 24043.0 34 27072.0 35 27976.0 36 25118.0 37 22618.0 38 11157.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.73501847721144 17.67798881379632 12.17748110663515 26.409511602357092 2 12.80263139319847 21.30157968293406 41.13923514547482 24.756553778392647 3 21.689092131603477 23.991045095432387 27.001291278900965 27.31857149406317 4 11.449787762168476 16.465483975261517 36.434613017948955 35.65011524462105 5 11.988803253583711 37.518933212830234 35.341718340739256 15.150545192846801 6 32.06522083534886 35.812313717372156 18.483250142055358 13.639215305223622 7 27.424104395911748 29.9115383693333 21.158671557228363 21.50568567752659 8 26.051191706998395 37.752069228671594 19.21523313277003 16.98150593155998 9 26.589911186055502 15.884500124335894 19.22922637663429 38.29636231297432 10 15.191577310380367 29.861046160843145 33.50791965361487 21.43945687516161 11 33.63368871565593 21.92479651496045 23.441667621995492 20.99984714738813 12 25.465173531196683 24.56364337268463 30.663094133947077 19.30808896217161 13 29.339076204640225 20.474967218849322 23.664235174442936 26.52172140206752 14 20.22370789406017 23.402086470998785 27.07361422326709 29.300591411673953 15 24.058773106738222 31.520758795468097 22.41894007155635 22.00152802623733 16 20.862701608401498 28.00170441435876 29.82315770670676 21.312436270532984 17 21.881106050869402 28.357408286999526 29.404040121818337 20.357445540312742 18 21.57216073928769 26.997359424688554 31.6652506528038 19.765229183219958 19 23.03117004594349 26.911515218208663 26.626683906939206 23.430630828908637 20 23.335557741577098 27.84243916364674 27.762433199565784 21.05956989521037 21 21.17578228211226 25.83852338353527 26.56343184992262 26.422262484429847 22 22.194808296437824 28.866680138886338 26.60022412874543 22.33828743593041 23 21.080919189719015 28.640818993683293 28.30011616931678 21.97814564728091 24 22.997133861212053 28.205709603056683 27.842718396394357 20.954438139336908 25 22.41118299244157 27.337232350955148 27.671044893158204 22.580539763445078 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 621.0 1 621.0 2 1130.5 3 1640.0 4 1640.0 5 1640.0 6 2240.0 7 2840.0 8 2840.0 9 2840.0 10 3385.0 11 3930.0 12 3930.0 13 3930.0 14 4708.0 15 5486.0 16 5486.0 17 5486.0 18 7805.0 19 10124.0 20 10124.0 21 10124.0 22 14236.5 23 18349.0 24 18349.0 25 18349.0 26 24990.0 27 31631.0 28 31631.0 29 31631.0 30 38962.5 31 46294.0 32 46294.0 33 46294.0 34 51533.5 35 56773.0 36 56773.0 37 56773.0 38 60084.5 39 63396.0 40 63396.0 41 63396.0 42 65981.5 43 68567.0 44 68567.0 45 68567.0 46 71909.5 47 75252.0 48 75252.0 49 75252.0 50 68357.5 51 61463.0 52 61463.0 53 61463.0 54 51902.0 55 42341.0 56 42341.0 57 42341.0 58 35282.0 59 28223.0 60 28223.0 61 28223.0 62 22779.0 63 17335.0 64 17335.0 65 17335.0 66 13397.5 67 9460.0 68 9460.0 69 9460.0 70 6992.0 71 4524.0 72 4524.0 73 4524.0 74 3271.0 75 2018.0 76 2018.0 77 2018.0 78 1423.5 79 829.0 80 829.0 81 829.0 82 553.5 83 278.0 84 278.0 85 278.0 86 193.0 87 108.0 88 108.0 89 108.0 90 72.0 91 36.0 92 36.0 93 36.0 94 21.0 95 6.0 96 6.0 97 6.0 98 3.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.861248356828792 2 35.78078962875663 3 5.079189519967364 4 13.074656633878789 5 13.331943248266171 6 14.163999818684555 7 9.241466841938262 8 7.5778976474321205 9 0.10842663523865645 10 1.1306831059335478 11 5.103123158515026 12 4.991070214405513 13 15.512624087756674 14 1.280993608630615 15 2.6988803771361227 16 2.5560038076243146 17 2.6580844023389694 18 1.1908798331897918 19 1.1404741398848646 20 0.2839399845881873 21 3.92819908435701 22 1.3045646162911926 23 0.10969584334345678 24 8.840578396264902 25 0.11187162866597163 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 551525.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.18543590692714 #Duplication Level Percentage of deduplicated Percentage of total 1 97.24805959158593 87.70358645370004 2 1.810729003487758 3.2660276897771845 3 0.38439066598213334 1.0399931931045818 4 0.1712486692061022 0.6177654352333399 5 0.0883038488648732 0.39818605510690014 6 0.05879220481855123 0.318132037169423 7 0.04147705653609558 0.2618438496690829 8 0.027873175090433962 0.2011003556512712 9 0.02464134331408622 0.2000061259301799 >10 0.11861969979830417 1.980491107587291 >50 0.012196393929227715 0.7576287878434044 >100 0.012196371514084639 2.054033942668128 >500 0.0010514113374128081 0.6097053942986416 >1k 4.205645349651232E-4 0.5914995722604861 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 1618 0.2933683876524183 No Hit GTACATGGGAAGCAGTGGTATCAAC 1501 0.27215448075789855 No Hit GTCCTACAGTGGACATTTCTAAATT 795 0.1441457776166085 No Hit GAGTACATGGGAAGCAGTGGTATCA 713 0.1292779112460904 No Hit CATGTACTCTGCGTTGATACCACTG 596 0.10806400435157064 No Hit CTGTAGGACGTGGAATATGGCAAGA 577 0.10461901092425546 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.8131544354290378E-4 0.0 4 0.0 0.0 0.0 1.8131544354290378E-4 0.0 5 0.0 0.0 0.0 1.8131544354290378E-4 0.0 6 0.0 0.0 0.0 1.8131544354290378E-4 0.0 7 0.0 0.0 0.0 1.8131544354290378E-4 0.0 8 0.0 0.0 0.0 1.8131544354290378E-4 0.0 9 0.0 0.0 0.0 1.8131544354290378E-4 0.0 10 0.0 0.0 0.0 1.8131544354290378E-4 0.0 11 0.0 0.0 0.0 3.6263088708580755E-4 0.0 12 0.0 0.0 0.0 3.6263088708580755E-4 0.0 13 0.0 1.8131544354290378E-4 0.0 3.6263088708580755E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCCTCG 45 4.1945554E-5 21.246086 2 GCTACAC 35 8.409328E-4 18.7556 3 GCCTCGG 55 2.9337662E-6 17.903074 3 GTAGGGC 50 2.5934474E-5 17.505228 3 CTCGGTC 45 3.3535823E-4 16.20152 5 CCCTTAG 60 4.5828478E-4 15.54838 1 TGTAGGA 505 0.0 15.416184 2 CGGTCTC 40 0.003321649 15.274964 7 GTCCTAG 70 8.5291016E-5 15.231065 1 TAGGACC 270 0.0 15.099658 4 CTGTAGG 505 0.0 15.0425625 1 TTCAAGG 55 0.00391628 14.899852 2 CCCTACA 65 6.9838326E-4 14.708829 2 GCCCACA 55 0.004610165 14.538744 1 TGTACTG 80 7.059243E-7 14.320987 5 TTATGTA 45 0.005136955 14.30494 4 CTACACT 75 4.1936437E-6 14.304939 4 TATACAC 270 0.0 13.777263 3 GTCCTAC 565 0.0 13.681002 1 TCCTACA 600 0.0 13.658197 2 >>END_MODULE