FastQCFastQC Report
Thu 26 May 2016
SRR1926536_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926536_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences551525
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC47770.8661438738044513No Hit
GTACATGGGAAGCAGTGGTATCAAC43890.7957934817098047No Hit
GAGTACATGGGAAGCAGTGGTATCA20180.3658945650695798No Hit
CTGTAGGACGTGGAATATGGCAAGA19710.35737273922306334No Hit
GTCCTACAGTGGACATTTCTAAATT18410.3338017315624858No Hit
CATGTACTCTGCGTTGATACCACTG16840.30533520692624994No Hit
GCGTTGATACCACTGCTTCCCATGT13740.24912741942794978No Hit
ACGCAGAGTACATGGGAAGCAGTGG13340.24187480168623363No Hit
GTCCTACAGTGTGCATTTCTCATTT12490.22646298898508682No Hit
GTATCAACGCAGAGTACATGGGAAG11800.21395222338062644No Hit
CTTTAGGACGTGAAATATGGCGAGG11790.21377090793708353No Hit
GTCCTAAAGTGTGTATTTCTCATTT11450.20760618285662483No Hit
CTGTAGGACCTGGAATATGGCGAGA10270.18621096051856217No Hit
GCTTCCCATGTACTCTGCGTTGATA9670.17533203390598795No Hit
GTATCAACGCAGAGTACTTTTTTTT9560.173337564027016No Hit
ACTCTGCGTTGATACCACTGCTTCC9410.17061783237387246No Hit
GATATACACTGTTCTACAAATCCCG8070.14632156293912335No Hit
GAGTAAGCAGTGGTATCAACGCAGA7500.13598658265717783No Hit
GGTATCAACGCAGAGTACATGGGAA7290.13217895834277685No Hit
TATCAACGCAGAGTACATGGGAAGC7100.12873396491546168No Hit
CTGAAGGACCTGGAATATGGCGAGA7050.12782738769774715No Hit
GTGTATATCAATGAGTTACAATGAA6550.11876161552060198No Hit
CAGTGGTATCAACGCAGAGTACATG6240.11314083677077195No Hit
GGTATCAACGCAGAGTACTTTTTTT6000.10878926612574226No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAGG402.8098165E-416.58374219
ATTAGAC350.002125198316.3341673
TTAAGAC350.002125198316.3341673
TAGGACC3450.015.1899874
TAGAGCT602.491502E-514.29239754
TAATAGT400.00517042214.2923964
TAGACAT400.00517042214.2923965
TTATACC400.00517042214.2923964
CCGGTTT551.9798448E-413.79890312
GGCGAGG3000.013.58287419
TCCAACG1051.4133548E-913.53774818
CCAACGA802.0491098E-613.03008319
TGTCGAG802.0491098E-613.03008318
TGTAGGA9300.012.8114792
CCTAGAC603.9834323E-412.7043533
CTGTAGG9100.012.6648311
GTAGGAC9450.012.5026963
TACACCT853.8017934E-612.3306945
CTAGACA550.002996669812.1268814
TAGGATC550.002996669812.1268814
Produced by FastQC (version 0.11.5)