FastQCFastQC Report
Thu 26 May 2016
SRR1926535_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926535_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences586362
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT14080.24012470112319695No Hit
CTGTAGGACGTGGAATATGGCAAGA10180.17361288760185686No Hit
GTCCTAAAGTGTGTATTTCTCATTT7870.1342174288238324No Hit
GTCCTACAGTGTGCATTTCTCATTT7200.12279104034708935No Hit
CCCATGTACTCTGCGTTGATACCAC6540.11153519498193949No Hit
CTTTAGGACGTGAAATATGGCGAGG6320.10778324652688953No Hit
GTACATGGGAAGCAGTGGTATCAAC5890.1004498927283828No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAGA350.00612965118.9421251
CTGGGCG250.00734436218.3599159
TAGGACC3400.018.2013284
AAACTGT555.0710278E-616.9052776
TCTAGGT452.4291873E-416.9014783
TCTACAC1051.0913936E-1116.5565513
TGTAGGA8300.016.5189762
GTCCTAA2650.016.5118161
TCATATA500.002161566616.2899992
CTCTATA500.002161566616.2899992
CTACACT1450.016.1885784
AGAGGTG453.5528943E-416.0796625
CTGTAGG7900.015.9449541
GTCCTAG1251.2369128E-1015.91138651
GCTCTAT500.00252917715.9113861
AAGGTGT400.003018223215.4965056
GTCCTAC7250.014.814051
GTAGGAC9100.014.5665813
ACTGTCC602.1045635E-514.5493388
AGGACCT4850.014.49301055
Produced by FastQC (version 0.11.5)