FastQCFastQC Report
Thu 26 May 2016
SRR1926535_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926535_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences586362
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA32940.5617690095879337No Hit
GTCCTACAGTGGACATTTCTAAATT30560.521179749028757No Hit
CTTTAGGACGTGAAATATGGCGAGG21110.3600165085732022No Hit
GTCCTAAAGTGTGTATTTCTCATTT19700.3359699298385639No Hit
GTCCTACAGTGTGCATTTCTCATTT19120.3260784293661595No Hit
GTACATGGGAAGCAGTGGTATCAAC18330.3126055235502983No Hit
CCCATGTACTCTGCGTTGATACCAC18100.30868303198365515No Hit
CTGTAGGACCTGGAATATGGCGAGA16460.28071396168237367No Hit
GATATACACTGTTCTACAAATCCCG15510.26451236608102163No Hit
CTGAAGGACCTGGAATATGGCGAGA12940.22068278640157446No Hit
GTATCAACGCAGAGTACTTTTTTTT11930.20345793213066332No Hit
GTGTATATCAATGAGTTACAATGAG10880.18555090541337943No Hit
GTGTATATCAATGAGTTACAATGAA10320.1760004911641614No Hit
GTTCTACAGTGTGGTTTTTATCATT10220.1742950600482296No Hit
GAGTACATGGGAAGCAGTGGTATCA8770.14956630886721856No Hit
GATATACACTGTTCTACAATGCCGG8330.14206241195711863No Hit
GGTATCAACGCAGAGTACTTTTTTT8050.1372872048325096No Hit
CTGTAGAACATATTAGATGAGTGAG7730.13182982526152787No Hit
ACCTGGAATATGGCGAGAAAACTGA7710.13148873903834152No Hit
GTTCTACAAATCCCGTTTCCAACGA7640.13029493725718924No Hit
TTGTAGAACAGTGTATATCAATGAG7470.1273957043601052No Hit
CATGTACTCTGCGTTGATACCACTG7240.12347321279346207No Hit
GTCCTTCAGTGTGCATTTCTCATTT6900.11767474699929395No Hit
GAATATGGCAAGAAAACTGAAAATC6770.1154576865485826No Hit
GACCTGGAATATGGCGAGAAAACTG6710.11443442787902353No Hit
ATTCCAGGTCCTTCAGTGTGCATTT6680.11392279854424399No Hit
ACAGTGGACATTTCTAAATTTTCCA6540.11153519498193949No Hit
TATCAACGCAGAGTACTTTTTTTTT6280.10710107408051682No Hit
CTGTAGGACATGGAATATGGCAAGA6100.10403129807183958No Hit
GCGTTGATACCACTGCTTCCCATGT6040.10300803940228051No Hit
GCCATATTCCACGTCCTACAGTGGA5980.10198478073272144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAGG607.903327E-817.36921919
TGTCGAG855.820766E-1116.71903418
TAGGACC5100.015.333584
TCGTCAA755.5251803E-815.2653322
TTACACC653.2313328E-614.6719054
GTCTAGG653.234214E-614.6706451
CGTCAAG851.6209015E-814.5881033
TAGGCTG602.4712226E-514.3051075
GTCGTCA801.2258351E-714.303881
CCAACGA955.0677045E-913.96186119
TAGGACA3050.013.7579174
TATGTCG903.7554855E-813.68483916
GTATTAA706.9669604E-613.6227441
GTCCTAT1009.506039E-913.3502871
ATAGGAC655.2391046E-513.2069823
TGTAGGA15300.013.1576192
CTGTAGG15050.012.9257651
ACTGTTC5400.012.842658
TCCAACG1700.012.81792518
ACCATCC604.0850928E-412.6667238
Produced by FastQC (version 0.11.5)