FastQCFastQC Report
Thu 26 May 2016
SRR1926534_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926534_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797517
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12860.16125048118096544No Hit
CTGTAGGACGTGGAATATGGCAAGA10100.1266430684236198No Hit
GTCCTAAAGTGTGTATTTCTCATTT9210.11548343170114242No Hit
GTCCTACAGTGTGCATTTCTCATTT8040.10081289803226766No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACTC400.00280243515.6711455
TGTAGGA8150.014.6655642
GTCTTAT550.00442547114.6304171
CTGTAGG8350.014.4552021
GTCCTAC8450.014.4428481
TAGGACC3500.014.4187644
TTTAGGA5200.014.2668312
TCCTACA8800.014.2067872
GTATTAA852.6462727E-514.20010951
GTATAGA852.6462727E-514.20010951
TACTAAA700.001171772113.738432
GTCCTAA4100.013.4112161
CCCTTAT700.001405586513.4112161
AGTAGTG550.001500316813.2967295
TGTAGAA2900.013.2646912
GGTATCA1758.913048E-1113.0280381
GTAGGAC8850.012.909173
TTGGGCT1004.3765613E-712.8848524
TCCTAAA4200.012.7571132
CTTTAGG4950.012.7338811
Produced by FastQC (version 0.11.5)