##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926534_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 797517 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.42861907645856 27.0 7.0 31.0 2.0 31.0 2 16.44368207825037 18.0 2.0 30.0 2.0 31.0 3 20.115518540670607 24.0 15.0 31.0 2.0 31.0 4 22.098291321689693 28.0 7.0 35.0 2.0 37.0 5 23.059618791825127 30.0 7.0 35.0 2.0 37.0 6 23.66693499950471 31.0 2.0 35.0 2.0 37.0 7 23.473192420976606 30.0 7.0 35.0 2.0 37.0 8 23.676454545796517 31.0 10.0 35.0 2.0 37.0 9 26.527640163156395 32.0 17.0 37.0 2.0 39.0 10 27.280170830214278 32.0 24.0 37.0 2.0 39.0 11 24.301125869417202 30.0 8.0 37.0 2.0 39.0 12 25.501777391579115 31.0 16.0 37.0 2.0 39.0 13 22.091498989990182 28.0 2.0 37.0 2.0 39.0 14 25.54839583356844 31.0 17.0 38.0 2.0 40.0 15 26.686919526480313 31.0 17.0 38.0 2.0 40.0 16 27.05574552015819 32.0 18.0 39.0 2.0 40.0 17 26.393740823079632 31.0 17.0 38.0 2.0 40.0 18 26.48470440128549 31.0 17.0 38.0 2.0 40.0 19 26.879651468244564 31.0 18.0 39.0 2.0 40.0 20 27.139294836348316 31.0 21.0 39.0 2.0 40.0 21 24.962468511643014 30.0 8.0 38.0 2.0 40.0 22 25.912206260180035 31.0 10.0 38.0 2.0 40.0 23 25.911540443651983 31.0 2.0 38.0 2.0 40.0 24 24.950915152905832 31.0 2.0 39.0 2.0 40.0 25 24.755962568822984 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.349512752875043 1101 2 11.0196040775717 1101 3 7.555422847509195 1101 4 9.1961478801169 1101 5 8.991972061728923 1101 6 8.329132131525355 1101 7 8.021528609694645 1101 8 6.662282676492573 1101 9 6.506418830245181 1101 10 5.955322494944056 1101 11 8.85055606770785 1101 12 6.316392198436063 1101 13 11.222902390273735 1101 14 8.58018482126172 1101 15 7.747193360891998 1101 16 7.523426924633426 1101 17 3.5887066976240796 1101 18 6.036597311635589 1101 19 6.705805253458223 1101 20 4.699478204017279 1101 21 1.325745398696558 1101 22 5.336246263398884 1101 23 6.584095134337812 1101 24 7.989324271678111 1101 25 8.615839159123869 1102 1 5.932108956281233 1102 2 10.72928199218672 1102 3 7.144014423062728 1102 4 8.339051473830917 1102 5 8.354651500684039 1102 6 7.055804793198622 1102 7 7.024191269942268 1102 8 7.227706475937669 1102 9 6.122378890341466 1102 10 5.4375003273105165 1102 11 8.234960642921422 1102 12 5.884795589270283 1102 13 10.255286392379297 1102 14 7.655039241299665 1102 15 5.590184138086116 1102 16 6.18358407422712 1102 17 6.943313165651322 1102 18 6.956237720904454 1102 19 5.442959672399521 1102 20 6.077497548880032 1102 21 5.094940569060753 1102 22 6.238470812950904 1102 23 6.634072653063068 1102 24 8.067711541793368 1102 25 7.598354758935244 1103 1 5.0662831444595575 1103 2 9.413843213992912 1103 3 6.429262138140906 1103 4 7.873476183250677 1103 5 7.1977742316909215 1103 6 6.517957377986775 1103 7 6.624282368575514 1103 8 6.39699105342709 1103 9 5.501949479581523 1103 10 4.644855457437863 1103 11 5.721707689671831 1103 12 4.574166059756763 1103 13 8.833168403381627 1103 14 6.807637043545139 1103 15 5.964542041841497 1103 16 5.754778469492159 1103 17 3.3187116543900643 1103 18 2.075411543061488 1103 19 4.05607787558743 1103 20 4.682126769308901 1103 21 6.741177463464968 1103 22 2.276007787879802 1103 23 4.670557655679438 1103 24 6.0807766041118825 1103 25 2.624064636901032 1104 1 5.9702436412488495 1104 2 10.600850963605968 1104 3 7.190013320398737 1104 4 7.929463522514489 1104 5 7.995878870211907 1104 6 7.408165123315317 1104 7 7.412906986316614 1104 8 7.344001802199308 1104 9 6.232876166206879 1104 10 5.178014910948221 1104 11 8.263598980863406 1104 12 5.1400388418517835 1104 13 9.596903213443806 1104 14 7.339716264192209 1104 15 6.588087583537842 1104 16 6.132004140152347 1104 17 6.514393936321735 1104 18 4.313740261617177 1104 19 5.000025733063101 1104 20 4.248078814896122 1104 21 3.5933601551032055 1104 22 5.5593870904681815 1104 23 5.6608709071428684 1104 24 6.680561842893152 1104 25 5.849778677785999 1105 1 3.4487908998358314 1105 2 9.561178566436052 1105 3 6.3123430827483595 1105 4 5.63557273309242 1105 5 2.1933892290926735 1105 6 4.6431976503041525 1105 7 5.8186963980643185 1105 8 3.184424456921864 1105 9 3.9489179279288784 1105 10 3.505248186542964 1105 11 6.670901707927804 1105 12 4.256255470978047 1105 13 5.1615979593699315 1105 14 4.021486492139196 1105 15 4.442664794619521 1105 16 4.865473802619032 1105 17 5.513379209884963 1105 18 3.2146986258327424 1105 19 2.7194079370530524 1105 20 1.9967404702112042 1105 21 3.0004295879919027 1105 22 4.509829626809427 1105 23 2.2821673621036744 1105 24 5.126193977204533 1105 25 6.142610352941407 1106 1 6.527427449852791 1106 2 11.33455600830947 1106 3 7.802796300528794 1106 4 9.101281464862211 1106 5 8.850858235840573 1106 6 8.104258280141707 1106 7 7.824970475100709 1106 8 7.624533698960306 1106 9 6.499840511872627 1106 10 5.773145302613898 1106 11 8.092628623080454 1106 12 5.260801822911549 1106 13 4.746469607568045 1106 14 5.937615638704756 1106 15 6.016136899427082 1106 16 6.035231779363336 1106 17 6.592037021095688 1106 18 6.442697593146185 1106 19 6.338795108453709 1106 20 5.741792433581203 1106 21 6.170309057272007 1106 22 6.425902076858279 1106 23 6.966784888414399 1106 24 7.7722264865822375 1106 25 6.675362715718119 1107 1 3.7843962558069997 1107 2 -14.034127265711884 1107 3 -5.797456510064418 1107 4 1.6365073180979621 1107 5 4.521311929035797 1107 6 4.699488653173919 1107 7 4.606549076099533 1107 8 5.269180844410833 1107 9 3.3325548979874178 1107 10 2.665975961734322 1107 11 5.484432394378754 1107 12 2.748603090937962 1107 13 4.257825472003955 1107 14 3.817467528451523 1107 15 3.393352655139534 1107 16 3.2529344810592526 1107 17 3.826788792384054 1107 18 -1.2355043225521598 1107 19 1.6081362363664304 1107 20 2.0905672458978977 1107 21 -1.4108597819598252 1107 22 1.954206184657906 1107 23 -1.072309860923152 1107 24 2.541934910042464 1107 25 3.414096129679322 1108 1 5.54985479267604 1108 2 11.039483845399229 1108 3 0.5762665456849803 1108 4 6.287721263201476 1108 5 7.7522125260738655 1108 6 7.898846061873616 1108 7 7.905701495266783 1108 8 7.612883837354929 1108 9 5.986715264739374 1108 10 4.958411217995469 1108 11 7.258474807343006 1108 12 3.065853226628434 1108 13 4.011376685464448 1108 14 5.468368900863712 1108 15 5.51644087332981 1108 16 5.6075998102055635 1108 17 0.9623489032625514 1108 18 4.359482883774895 1108 19 5.283904341352088 1108 20 5.495954157582787 1108 21 5.8628308049983815 1108 22 2.846092863730558 1108 23 5.313292895531838 1108 24 6.125518301528857 1108 25 6.523457318327846 1109 1 3.137801221247468 1109 2 -14.034127265711884 1109 3 -5.874874327330891 1109 4 1.6287926917729045 1109 5 1.3713097482960883 1109 6 2.3329234428259973 1109 7 3.3860586381239237 1109 8 3.6412072500533412 1109 9 3.0395922488663594 1109 10 2.0949191545034047 1109 11 0.46973472797792937 1109 12 1.149881004406211 1109 13 2.3841941457819082 1109 14 2.9902439008637103 1109 15 3.018673016186952 1109 16 2.9529867149674693 1109 17 3.42623779215144 1109 18 3.5524888361558453 1109 19 1.9156999762727231 1109 20 1.0558747925034204 1109 21 0.15682882087140015 1109 22 2.328334927222624 1109 23 2.7055548002937435 1109 24 3.844863539624093 1109 25 4.054112080232606 1110 1 3.441928900917123 1110 2 9.180632881889593 1110 3 6.4801846473659985 1110 4 7.8424798732875765 1110 5 8.478955043499042 1110 6 3.8788429868428658 1110 7 6.627972405475884 1110 8 7.19920507556731 1110 9 5.261359073677465 1110 10 4.993194940832698 1110 11 2.439788870483639 1110 12 5.436896687063037 1110 13 10.000696078658379 1110 14 7.48217578893259 1110 15 6.560852004941125 1110 16 6.425627177979244 1110 17 6.655981027083794 1110 18 6.89902777922385 1110 19 5.568993004738722 1110 20 6.041536363404845 1110 21 3.858520636896703 1110 22 5.952022548027401 1110 23 6.28605581066099 1110 24 7.429116087506717 1110 25 7.6491568878235405 1111 1 5.0926250948479 1111 2 9.662048371681884 1111 3 7.544268985093229 1111 4 8.430243690019228 1111 5 8.564165626483057 1111 6 7.863635958002039 1111 7 7.733079918307386 1111 8 7.865641942485553 1111 9 6.386398535120236 1111 10 5.478780276856028 1111 11 7.2671071649130425 1111 12 6.583928454831142 1111 13 8.64781042798884 1111 14 6.438647465547376 1111 15 5.970023262376081 1111 16 5.682003728996875 1111 17 6.898587886580149 1111 18 6.631815272821164 1111 19 5.627740900999555 1111 20 5.096748932527703 1111 21 7.968500461907418 1111 22 7.251691699109845 1111 23 7.217489071169229 1111 24 7.17643898141554 1111 25 7.3790817281484316 1112 1 -2.1302150531813773 1112 2 7.6652946996060365 1112 3 5.683664586789671 1112 4 -0.39256534566172263 1112 5 3.906082147140019 1112 6 3.2200647516616705 1112 7 5.707375387366977 1112 8 6.09828043337291 1112 9 4.607167255747722 1112 10 3.1755515519710613 1112 11 -0.11914240524530229 1112 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2204 7 4.974513684321508 2204 8 5.010790139179139 2204 9 3.4802994272601033 2204 10 3.011292644197791 2204 11 4.628168008006359 2204 12 4.623854885003226 2204 13 3.0214098529603 2204 14 4.006304224246797 2204 15 4.188238137011403 2204 16 4.09730752164835 2204 17 -0.7739798695401028 2204 18 2.606000296710217 2204 19 3.2401336116671793 2204 20 3.5987319353605614 2204 21 5.178730473323423 2204 22 4.363111935040674 2204 23 3.275886179113929 2204 24 5.069921286603481 2204 25 5.0038988606164025 2205 1 5.8760963446914865 2205 2 10.419891561225118 2205 3 7.1575054828480695 2205 4 7.639207203341471 2205 5 7.855662119766038 2205 6 7.356995349829202 2205 7 7.185370515300576 2205 8 6.8454216521429245 2205 9 5.617386980082525 2205 10 5.114868897615715 2205 11 7.434231103102924 2205 12 6.4298185496632705 2205 13 9.515411603800597 2205 14 7.210506240955436 2205 15 6.274096859330292 2205 16 5.6552992429837445 2205 17 6.424932880251848 2205 18 6.174484090629832 2205 19 4.997448773727982 2205 20 5.198007822398942 2205 21 -1.258066893931538 2205 22 3.5945832893480656 2205 23 4.913007776053199 2205 24 6.816919460109595 2205 25 7.264932396773418 2206 1 4.362448076258129 2206 2 8.969426394558194 2206 3 6.3172565362085535 2206 4 2.093056195681932 2206 5 6.085539936640082 2206 6 6.57156283515009 2206 7 6.565096484605803 2206 8 6.486945078766915 2206 9 5.068998787601256 2206 10 3.955571251162965 2206 11 4.062851732242322 2206 12 5.0235705921816205 2206 13 8.30676877264758 2206 14 0.8344492135858168 2206 15 2.900569338148575 2206 16 3.723675680852331 2206 17 4.914577344390246 2206 18 -0.6490814374759957 2206 19 1.5863311951781967 2206 20 2.888852759809744 2206 21 3.952326777516742 2206 22 -1.851991121107158 2206 23 -2.389394560047407 2206 24 1.9309731700434263 2206 25 3.404502982862315 2207 1 2.06808776104587 2207 2 -14.034127265711884 2207 3 -6.060559285620776 2207 4 -4.004173505654972 2207 5 1.8859310986610502 2207 6 2.6522828015329836 2207 7 3.809457577991843 2207 8 3.9477277140783684 2207 9 1.8815196037499184 2207 10 1.4681942674682986 2207 11 -0.29556006694734194 2207 12 -1.379544178075541 2207 13 1.4344504891951857 2207 14 -4.620256403272542 2207 15 -0.6046205621933041 2207 16 0.5285091776021602 2207 17 1.549519422321758 2207 18 1.5900181635802468 2207 19 1.0213080315386236 2207 20 0.8542063879153083 2207 21 -2.888983874644765 2207 22 -3.504809407153463 2207 23 -0.5675009000886 2207 24 1.0994234110179057 2207 25 1.474445761150232 2208 1 4.667271574523539 2208 2 6.630696507612235 2208 3 6.278785646506606 2208 4 8.20561316598885 2208 5 3.4508169183029977 2208 6 6.027857234414505 2208 7 6.531607104573393 2208 8 6.58214879166653 2208 9 4.900614231951231 2208 10 4.624658434458649 2208 11 6.662208586042233 2208 12 5.964691819886514 2208 13 8.806013097965408 2208 14 2.868328587571842 2208 15 4.538821949118798 2208 16 5.017575910182529 2208 17 5.795284382798759 2208 18 0.7006139595638814 2208 19 3.4631549940434816 2208 20 4.1786904702603564 2208 21 6.884391697265908 2208 22 6.021679326143214 2208 23 2.180306544460656 2208 24 3.4263303264079177 2208 25 4.750627037286613 2209 1 3.419187565052006 2209 2 5.025708186793725 2209 3 5.423029094509047 2209 4 0.38660713954154247 2209 5 5.214580597211572 2209 6 5.994373985084785 2209 7 6.284096845553496 2209 8 5.032884045116916 2209 9 4.718693470506846 2209 10 3.628872657370522 2209 11 -0.3011882027126731 2209 12 3.3966788727682165 2209 13 2.4274034452084834 2209 14 4.11059652489762 2209 15 4.54307855719917 2209 16 3.863369880013593 2209 17 1.5720789165593203 2209 18 3.80808672321643 2209 19 4.404141917185214 2209 20 4.549517275674699 2209 21 6.586043013092791 2209 22 5.349503276761389 2209 23 1.0916259172221885 2209 24 -2.340057614701511 2209 25 0.7467074429850378 2210 1 -1.5203618739906268 2210 2 -14.034127265711884 2210 3 -7.548027898759463 2210 4 -1.8611954034651887 2210 5 0.1286347482960899 2210 6 -0.6965872714597197 2210 7 -0.5219818380665515 2210 8 -21.169060607089516 2210 9 -12.837312513038404 2210 10 -6.519799893115643 2210 11 -9.804214081545881 2210 12 -4.099368995593789 2210 13 -4.767067758979998 2210 14 -3.501564432469621 2210 15 -5.137642460003523 2210 16 -4.799683523127769 2210 17 -4.212845541181892 2210 18 -4.336650449558441 2210 19 -10.055567880870136 2210 20 -6.486068064639436 2210 21 -4.9386247505571745 2210 22 -9.506362691824997 2210 23 -7.528657104468163 2210 24 -10.238281698471145 2210 25 -7.645659348338821 2211 1 0.3043922894929487 2211 2 5.2072783874584765 2211 3 0.8665193892466476 2211 4 1.9091514261757574 2211 5 1.5869442975703443 2211 6 0.39388362235235164 2211 7 -20.99638183806655 2211 8 -13.35775426637141 2211 9 -9.196624965292031 2211 10 -7.152995920617553 2211 11 -9.863817442890419 2211 12 -4.834195580773315 2211 13 -7.019278606955551 2211 14 -4.902378489001325 2211 15 -5.46058516435799 2211 16 -11.756019046427998 2211 17 -7.814443707721235 2211 18 -5.8809169125072565 2211 19 -8.799934573001533 2211 20 -6.908983878237603 2211 21 -4.629040640549533 2211 22 -6.239647947745546 2211 23 -8.19980393410911 2211 24 -22.549481698471144 2211 25 -22.37445934833882 2212 1 -0.8715771996611252 2212 2 -14.034127265711884 2212 3 -7.841094565426129 2212 4 -19.68519540346519 2212 5 -20.55056525170391 2212 6 -21.146987271459718 2212 7 -10.017071493238964 2212 8 -9.500095089848138 2212 9 -5.063596037942617 2212 10 -5.42046809234936 2212 11 -5.925145882312165 2212 12 -2.948227233141683 2212 13 -6.50184170534015 2212 14 -3.9001176891746034 2212 15 -3.9055658316510318 2212 16 -4.060575477150756 2212 17 -4.404676958806412 2212 18 -3.165996043428173 2212 19 -3.82761385788163 2212 20 -4.099352355827175 2212 21 -1.8917190034307367 2212 22 -3.0685144159629267 2212 23 -3.3732731964221863 2212 24 -2.3594433842948987 2212 25 -2.8687122219020402 2213 1 -19.035561873990627 2213 2 -14.034127265711884 2213 3 -9.0019972299177 2213 4 -6.336908290904017 2213 5 -20.55056525170391 2213 6 -11.22773767929006 2213 7 -20.99638183806655 2213 8 -11.125830590776303 2213 9 -8.889319038323883 2213 10 -7.972581622316294 2213 11 -7.131923053813416 2213 12 -5.8785141831957475 2213 13 -19.583067758979997 2213 14 -13.04126948956587 2213 15 -7.810112280558172 2213 16 -7.071074061463818 2213 17 -5.7006824090448625 2213 18 -5.712757464240333 2213 19 -6.669649446938649 2213 20 -6.642214231034217 2213 21 -4.824596691829605 2213 22 -5.528732675511783 2213 23 -5.4170394861973605 2213 24 -4.4026627751432486 2213 25 -5.323888385859213 2214 1 -19.035561873990627 2214 2 -14.034127265711884 2214 3 -12.04699107565477 2214 4 -19.68519540346519 2214 5 -20.55056525170391 2214 6 -21.146987271459718 2214 7 -16.41334934709182 2214 8 -14.657327755103957 2214 9 -13.397854029283891 2214 10 -13.259333467122863 2214 11 -14.044042240390645 2214 12 -11.80194661292231 2214 13 -19.583067758979997 2214 14 -16.671552880123052 2214 15 -24.285642460003523 2214 16 -18.17697449785701 2214 17 -24.000845541181892 2214 18 -17.81157608132739 2214 19 -14.697365714805153 2214 20 -13.08014893106543 2214 21 -10.691957602542733 2214 22 -11.738930200850268 2214 23 -11.725493205551194 2214 24 -10.485402276088474 2214 25 -10.273376315847846 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 138179.0 3 3.0 4 499.0 5 2326.0 6 3280.0 7 3220.0 8 2791.0 9 2617.0 10 2376.0 11 2152.0 12 2198.0 13 2393.0 14 3756.0 15 8072.0 16 12285.0 17 12937.0 18 13527.0 19 15545.0 20 18224.0 21 21001.0 22 21425.0 23 23500.0 24 24677.0 25 25270.0 26 21093.0 27 15930.0 28 17089.0 29 20178.0 30 19799.0 31 23155.0 32 29607.0 33 37715.0 34 46166.0 35 53666.0 36 53231.0 37 56154.0 38 41481.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.90913428250586 18.688216024995782 12.798264957613064 25.604384734885286 2 13.98491627456683 19.655655998629477 42.71252076701501 23.646906959788687 3 19.37128008496937 23.775392281948683 28.86674103713402 27.98658659594792 4 12.12127407584814 16.487310208057387 38.225780306470995 33.16563540962348 5 12.467467365359338 37.360288181582376 37.01419632370299 13.158048129355294 6 31.453276123648777 38.012190641001325 17.945488810923575 12.589044424426325 7 28.389291799705802 30.707673452297936 21.919395961895464 18.9836387861008 8 24.073474782306008 38.71621731870618 20.048379464698492 17.16192843428932 9 26.221566658297768 15.495982927441625 19.955561134822997 38.32688927943761 10 14.560029971022914 29.498138279571535 34.861192877294656 21.080638872110892 11 33.579232234138516 21.61157328522268 25.51321372490061 19.2959807557382 12 24.19189841771814 23.985165439801705 32.19318582463488 19.629750317845275 13 28.7938570050639 21.515553412105138 25.276555341210518 24.41403424162045 14 20.04302974309763 22.889347714012995 27.388712221365825 29.67891032152355 15 22.047205380409327 32.675144071897506 23.17493834732002 22.10271220037315 16 21.284682876290997 27.88862628290219 29.2425939715738 21.58409686923301 17 20.82173035210548 28.61983912544671 29.374932904265545 21.183497618182265 18 20.67748504047646 27.43040458609966 31.829199408886844 20.062910964537036 19 23.41299172660587 26.24419511548598 26.875659501149507 23.467153656758644 20 23.20499728353824 27.993892991528664 28.414441515584443 20.386668209348652 21 21.917017609512616 26.304110643187318 26.51704817975301 25.261823567547054 22 22.48472302343746 28.971631024642264 27.787250552625803 20.756395399294476 23 21.851117248305297 28.215415515942755 28.346974642229476 21.586492593522472 24 22.9935095116364 26.857646381815563 28.900517911091928 21.248326195456105 25 22.42552673304367 27.52143275907307 28.797166820871222 21.25587368701204 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 769.0 1 769.0 2 1763.5 3 2758.0 4 2758.0 5 2758.0 6 3996.5 7 5235.0 8 5235.0 9 5235.0 10 6031.5 11 6828.0 12 6828.0 13 6828.0 14 8108.0 15 9388.0 16 9388.0 17 9388.0 18 13340.0 19 17292.0 20 17292.0 21 17292.0 22 24570.0 23 31848.0 24 31848.0 25 31848.0 26 42420.5 27 52993.0 28 52993.0 29 52993.0 30 64745.0 31 76497.0 32 76497.0 33 76497.0 34 82429.5 35 88362.0 36 88362.0 37 88362.0 38 91857.5 39 95353.0 40 95353.0 41 95353.0 42 95747.5 43 96142.0 44 96142.0 45 96142.0 46 94097.5 47 92053.0 48 92053.0 49 92053.0 50 84897.0 51 77741.0 52 77741.0 53 77741.0 54 67719.0 55 57697.0 56 57697.0 57 57697.0 58 48609.0 59 39521.0 60 39521.0 61 39521.0 62 31862.5 63 24204.0 64 24204.0 65 24204.0 66 18686.0 67 13168.0 68 13168.0 69 13168.0 70 9594.0 71 6020.0 72 6020.0 73 6020.0 74 4181.0 75 2342.0 76 2342.0 77 2342.0 78 1620.0 79 898.0 80 898.0 81 898.0 82 594.0 83 290.0 84 290.0 85 290.0 86 187.5 87 85.0 88 85.0 89 85.0 90 57.5 91 30.0 92 30.0 93 30.0 94 15.5 95 1.0 96 1.0 97 1.0 98 1.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.797950388518366 2 37.054884096514556 3 6.145574326315302 4 14.138131224788939 5 13.953683745926417 6 15.369327550384506 7 9.559420049980126 8 7.784285476046279 9 0.11498187499451422 10 1.5998405049672921 11 6.174413836946423 12 6.109587632614728 13 16.801773504514635 14 1.739524047763245 15 3.089463923653038 16 3.1776125148429437 17 3.0554834567789775 18 1.6594003638793906 19 1.6091193040399139 20 0.2965454027939216 21 4.841150721552017 22 1.7535676355488348 23 0.11498187499451422 24 9.35566263791242 25 0.11987205288413914 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 797517.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.80254201808675 #Duplication Level Percentage of deduplicated Percentage of total 1 95.84725578273509 86.07327214747376 2 2.996939007939638 5.382654823722851 3 0.5328663281517521 1.4355825247161396 4 0.19998411319016848 0.718363269108397 5 0.11169266797659916 0.501514275454038 6 0.06788400836572296 0.3657693908171394 7 0.04638855531582899 0.2916067131535653 8 0.032238640108738614 0.23160894663767811 9 0.025459974910836874 0.2057733420038877 >10 0.11997171067988875 2.0169450555539052 >50 0.01031389970319523 0.6261593512266062 >100 0.008133829243662008 1.456833565374547 >500 5.809877859186575E-4 0.394436801230583 >1k 2.904938929593287E-4 0.29947979352692417 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1286 0.16125048118096544 No Hit CTGTAGGACGTGGAATATGGCAAGA 1010 0.1266430684236198 No Hit GTCCTAAAGTGTGTATTTCTCATTT 921 0.11548343170114242 No Hit GTCCTACAGTGTGCATTTCTCATTT 804 0.10081289803226766 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACTC 40 0.002802435 15.671145 5 TGTAGGA 815 0.0 14.665564 2 GTCTTAT 55 0.004425471 14.630417 1 CTGTAGG 835 0.0 14.455202 1 GTCCTAC 845 0.0 14.442848 1 TAGGACC 350 0.0 14.418764 4 TTTAGGA 520 0.0 14.266831 2 TCCTACA 880 0.0 14.206787 2 GTATTAA 85 2.6462727E-5 14.2001095 1 GTATAGA 85 2.6462727E-5 14.2001095 1 TACTAAA 70 0.0011717721 13.73843 2 GTCCTAA 410 0.0 13.411216 1 CCCTTAT 70 0.0014055865 13.411216 1 AGTAGTG 55 0.0015003168 13.296729 5 TGTAGAA 290 0.0 13.264691 2 GGTATCA 175 8.913048E-11 13.028038 1 GTAGGAC 885 0.0 12.90917 3 TTGGGCT 100 4.3765613E-7 12.884852 4 TCCTAAA 420 0.0 12.757113 2 CTTTAGG 495 0.0 12.733881 1 >>END_MODULE