FastQCFastQC Report
Thu 26 May 2016
SRR1926534_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926534_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797517
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA27250.34168550639045936No Hit
GTCCTACAGTGGACATTTCTAAATT26600.3335352099077512No Hit
CTTTAGGACGTGAAATATGGCGAGG21020.26356804933311767No Hit
GTCCTAAAGTGTGTATTTCTCATTT19710.2471420671910442No Hit
GTCCTACAGTGTGCATTTCTCATTT17530.21980722667980745No Hit
CTGTAGGACCTGGAATATGGCGAGA14070.17642257155646837No Hit
GTATCAACGCAGAGTACTTTTTTTT12580.15773958423456805No Hit
CTGAAGGACCTGGAATATGGCGAGA12250.15360174140488542No Hit
GATATACACTGTTCTACAAATCCCG10620.13316330560978637No Hit
GTGTATATCAATGAGTTACAATGAA8270.1036968490953798No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC400.005337547514.22515610
GATATAC4500.014.1874321
TAGGACC4550.013.4057844
GGACCTA500.001462454913.3429446
CCAACGT500.001526175813.26765419
TCCTATA1501.8189894E-1212.713982
CCAACGA1207.6579454E-1012.63586119
TCCAACG1600.012.43842618
ATAGGAC1001.3718636E-712.3953483
TAATGAC550.002983137712.1353053
ACTGTTC4400.012.0948718
GTCCTAT1353.5106495E-1011.999321
TATACAC6200.011.9954983
ATACACT5050.011.8897534
TGTAGGA15100.011.80884552
AGGACCT8750.011.5457325
CTGTAGG15150.011.5101231
GGCGAGG5150.011.22506219
TTTATAC855.1202176E-511.2175093
AGGACGT14300.011.1968775
Produced by FastQC (version 0.11.5)