FastQCFastQC Report
Thu 26 May 2016
SRR1926533_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926533_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences668340
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC17380.26004728132387706No Hit
GTACATGGGAAGCAGTGGTATCAAC16740.2504713169943442No Hit
GTCCTACAGTGGACATTTCTAAATT8580.12837777179280008No Hit
GAGTACATGGGAAGCAGTGGTATCA8260.12358978962803363No Hit
CTGTAGGACGTGGAATATGGCAAGA7030.10518598318221264No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAC450.001173583117.84721
GTTTAGG450.001173583117.84721
TGTAGGA5950.016.8464892
TTAACCC400.002291936716.1497764
CTCTTAG500.002375231116.0624791
TAGGACC2650.015.8450654
GTCCTAC5100.015.7475291
TAGCCTG601.0428217E-515.670515
CTGTAGG6300.015.0851341
GTAGAAC2850.015.0294253
TGTAGAA2450.014.5717172
CTATAAG658.1431284E-414.4150461
GTAGGAC6600.014.1448273
TCCTACA5600.013.9762382
TAGAACA2950.013.5037684
TAAACAG704.4679462E-513.4318655
GTATTAC600.00797584313.38541
GTCCTAA2200.013.38541
GTGTAGG600.00797584313.38541
TTTAGGA3550.013.1508562
Produced by FastQC (version 0.11.5)