FastQCFastQC Report
Thu 26 May 2016
SRR1926533_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926533_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences668340
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC49430.7395936200137654No Hit
GTACATGGGAAGCAGTGGTATCAAC47230.7066762426309962No Hit
GAGTACATGGGAAGCAGTGGTATCA21200.3172038184157764No Hit
CTGTAGGACGTGGAATATGGCAAGA19190.28712930544333726No Hit
CATGTACTCTGCGTTGATACCACTG18790.2811443277373792No Hit
GTCCTACAGTGGACATTTCTAAATT18570.2778525899991023No Hit
GCGTTGATACCACTGCTTCCCATGT15110.22608253284256513No Hit
GTATCAACGCAGAGTACTTTTTTTT14400.21545919741448963No Hit
GTCCTACAGTGTGCATTTCTCATTT14030.20992309303647844No Hit
CTTTAGGACGTGAAATATGGCGAGG13710.20513511087171202No Hit
GTATCAACGCAGAGTACATGGGAAG13320.19929975760840293No Hit
GTCCTAAAGTGTGTATTTCTCATTT13320.19929975760840293No Hit
ACGCAGAGTACATGGGAAGCAGTGG13280.1987012598378071No Hit
GATATACACTGTTCTACAAATCCCG12100.18104557560523088No Hit
CTGTAGGACCTGGAATATGGCGAGA11190.1674297513241763No Hit
ACTCTGCGTTGATACCACTGCTTCC10580.1583026603225903No Hit
GCTTCCCATGTACTCTGCGTTGATA9190.1375048627943861No Hit
GTGTATATCAATGAGTTACAATGAA8840.1322680073016728No Hit
GGTATCAACGCAGAGTACTTTTTTT8710.13032288954723645No Hit
TATCAACGCAGAGTACATGGGAAGC8490.12703115180895952No Hit
GTGTATATCAATGAGTTACAATGAG7810.11685668970883084No Hit
CTGAAGGACCTGGAATATGGCGAGA7760.11610856749558608No Hit
GGTATCAACGCAGAGTACATGGGAA7750.11595894305293712No Hit
TATCAACGCAGAGTACTTTTTTTTT7310.10937546757638329No Hit
CAGTGGTATCAACGCAGAGTACATG6930.10368973875572313No Hit
GTTCTACAGTGTGGTTTTTATCATT6780.10144537211598888No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6770.10129574767333992No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCCA307.6918525E-419.0090059
TTAGACC400.005167385614.2942974
CTCGAAC400.00536131214.21515318
TATGTCG400.00536131214.21515316
AATCCCG2800.013.538239519
GATATAC4600.013.4666551
GTCATAG500.001462512613.3423471
TAGACTA655.2870993E-513.1947365
TCCAACG1153.9108272E-1013.18506818
CCAACGA957.601375E-812.96820819
TAGGACC4800.012.9045734
TAGAGTG1051.9013896E-812.7060425
GTCCTAA3600.012.4422681
GTATAAA1001.3792851E-712.3893221
TCTTAGA701.0520535E-412.2596342
TGGCCAC701.1057512E-412.19445110
TATACAC5400.012.1793733
AAATCCC3350.012.16420918
GGACCTG7000.011.9799816
ACTGTTC4300.011.9358878
Produced by FastQC (version 0.11.5)