FastQCFastQC Report
Thu 26 May 2016
SRR1926532_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926532_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844641
Sequences flagged as poor quality0
Sequence length25
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGAC300.00769741318.211763
TAGGACC1500.016.440994
GTTATAA902.9346684E-614.8073861
TAAGACT805.0943163E-714.74327854
GTATAAG658.424802E-414.3517741
CTAAGAC702.9299272E-514.0490723
GTATAAT955.140442E-614.0280491
TGTAGGA4450.013.8064072
GTAGGAC4400.013.4104773
GTCCTAT904.810359E-513.3266481
GTCCAGA904.810359E-513.3266481
GTATTAG700.001474515513.3266471
GTCCTAC4650.012.8967551
GTATAGA1254.766316E-712.7935821
ATTGAGC600.002060050612.7482323
GTATCAA5400.012.5862781
TTAGGAG704.604905E-412.4880643
TAATACT953.5230933E-612.4153924
TAAACCA600.002960688712.1468425
ACTATAC902.278534E-512.1411733
Produced by FastQC (version 0.11.5)