FastQCFastQC Report
Thu 26 May 2016
SRR1926532_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926532_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844641
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14380.1702498457924728No Hit
CTGTAGGACGTGGAATATGGCAAGA14380.1702498457924728No Hit
GTCCTACAGTGGACATTTCTAAATT14140.1674084019127653No Hit
CCCATGTACTCTGCGTTGATACCAC10810.12798336808182412No Hit
CTTTAGGACGTGAAATATGGCGAGG10270.1215901193524823No Hit
GTCCTAAAGTGTGTATTTCTCATTT9990.11827510149282358No Hit
GTACATGGGAAGCAGTGGTATCAAC9960.11791992100786014No Hit
GATATACACTGTTCTACAAATCCCG9490.112355426743433No Hit
GGTATCAACGCAGAGTACTTTTTTT8920.10560699752912776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1006.8394E-1014.21319419
GTACCGT500.001458552213.3478266
TCCAACG1351.8189894E-1213.33583518
TAGGACC2800.013.2797254
GTCCTAC7900.012.4246951
TTGGACT853.7823356E-612.3383264
CGTTTCC1103.898458E-812.06968114
CTAGGCT802.7594277E-511.9177024
AGACCGT657.778862E-411.7343526
AGGACGT7000.011.7133985
GGACGTG6850.011.6915256
GGCGAGG3700.011.5242119
GTAGGAC7250.011.3115093
CTAGGAC1354.441972E-911.3017663
TTTAGGA4400.011.2703292
TCCTACA8100.011.1847052
ATTTCTA5600.011.1681715
TAGCCTA600.005717028411.1231885
TTACACC951.3050487E-511.0395554
TGTAGGA7550.010.9890032
Produced by FastQC (version 0.11.5)