FastQCFastQC Report
Thu 26 May 2016
SRR1926531_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926531_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533354
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGA23840.4469826794211725No Hit
GCTTACTCTGCGTTGATACCACTGC20060.37611042572100334No Hit
GTACATGGGAAGCAGTGGTATCAAC19090.3579236304593197No Hit
CCCATGTACTCTGCGTTGATACCAC18710.3507989065423715No Hit
CTGCTTACTCTGCGTTGATACCACT11950.2240538179145558No Hit
GCGTTGATACCACTGCTTACTCTGC9610.18018051800492732No Hit
GAGTACATGGGAAGCAGTGGTATCA9600.17999302527027078No Hit
GTCCTACAGTGGACATTTCTAAATT9100.17061838853744418No Hit
CATGTACTCTGCGTTGATACCACTG7110.13330733434079428No Hit
CTGTAGGACGTGGAATATGGCAAGA6910.12955747964766365No Hit
GCGTTGATACCACTGCTTCCCATGT6580.12337021940399809No Hit
GTATCAACGCAGAGTAAGCAGTGGT5900.11062071344735391No Hit
GNGTAAGCAGTGGTATCAACGCAGA5580.10462094593834488No Hit
GTCCTAAAGTGTGTATTTCTCATTT5550.1040584677343753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATA2800.016.2642631
TCTGCGG701.9958861E-716.0094747
CTGTAGA1301.9099389E-1015.4547281
GATATAC1750.015.3075411
AAGTGTC400.00331147315.2817737
CCCTGTA902.1923988E-615.2240172
CCCCTGT801.50759515E-515.068361
CTGCGGT652.5698846E-614.9966038
CCAACGT406.421121E-414.87546219
TGTAGGA5100.014.7762532
ACTCCAG400.004442295514.6216888
TAGACAG507.478297E-414.5782215
GTCCTAC5500.014.3682151
CGGTGAT654.6285077E-614.17022111
TCCTACA5900.014.1660772
TAGGACC1750.014.0824694
CTGTAGG4900.013.9407961
TGTATAT3000.013.7016162
TCTTACA600.006829607813.7016152
CTGCGCT500.001232217213.6469088