FastQCFastQC Report
Thu 26 May 2016
SRR1926531_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926531_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533354
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGA66631.2492640910164732No Hit
CCCATGTACTCTGCGTTGATACCAC51620.9678374962970184No Hit
GTACATGGGAAGCAGTGGTATCAAC48970.9181519216130375No Hit
GCTTACTCTGCGTTGATACCACTGC42860.8035938607378964No Hit
CTGCTTACTCTGCGTTGATACCACT28330.5311669172819553No Hit
GAGTACATGGGAAGCAGTGGTATCA23470.44004544823888075No Hit
GCGTTGATACCACTGCTTACTCTGC20370.3819227004953558No Hit
CATGTACTCTGCGTTGATACCACTG19580.36711077445748974No Hit
CTGTAGGACGTGGAATATGGCAAGA17770.33317458948465745No Hit
GTCCTACAGTGGACATTTCTAAATT17200.3224875036092351No Hit
GCGTTGATACCACTGCTTCCCATGT16450.30842554850999526No Hit
GTATCAACGCAGAGTAAGCAGTGGT15370.28817633316708974No Hit
ACGCAGAGTACATGGGAAGCAGTGG14130.2649272340696798No Hit
GTATCAACGCAGAGTACATGGGAAG13680.25649006101013583No Hit
ACGCAGAGTAAGCAGTGGTATCAAC13640.2557400900715097No Hit
GTATCAACGCAGAGTACTTTTTTTT13590.25480262639822704No Hit
CTTTAGGACGTGAAATATGGCGAGG12040.2257412525264646No Hit
GTCCTAAAGTGTGTATTTCTCATTT11670.21880402134417293No Hit
GCTTCCCATGTACTCTGCGTTGATA11320.21224177563119428No Hit
ACTCTGCGTTGATACCACTGCTTCC11030.20680448632615483No Hit
GTCCTACAGTGTGCATTTCTCATTT10550.19780483506264135No Hit
TATCAACGCAGAGTAAGCAGTGGTA10310.19330500943088455No Hit
GTGGTATCAACGCAGAGTAAGCAGT9900.18561780730996674No Hit
ACTCTGCGTTGATACCACTGCTTAC9520.17849308339301853No Hit
TATCAACGCAGAGTACATGGGAAGC9090.17043089580278764No Hit
GGTATCAACGCAGAGTAAGCAGTGG9080.1702434030681311No Hit
GTAAGCAGTGGTATCAACGCAGAGT8830.16555608470171781No Hit
GGTATCAACGCAGAGTACATGGGAA8520.1597438099273653No Hit
CTGTAGGACCTGGAATATGGCGAGA8370.15693141890751733No Hit
GGTATCAACGCAGAGTACTTTTTTT8220.15411902788766935No Hit
CTGAAGGACCTGGAATATGGCGAGA7620.14286946380827742No Hit
GATATACACTGTTCTACAAATCCCG7590.14230698560430782No Hit
AAGCAGTGGTATCAACGCAGAGTAA7060.13236987066751163No Hit
CCACTGCTTACTCTGCGTTGATACC7040.13199488519819857No Hit
TATCAACGCAGAGTACTTTTTTTTT6980.13086992879025938No Hit
CAGTGGTATCAACGCAGAGTACATG6700.12562013221987647No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6110.11455806087514109No Hit
CATGGGAAGCAGTGGTATCAACGCA6060.11362059720185842No Hit
GTGTATATCAATGAGTTACAATGAA5800.10874578610078858No Hit
TGCTTACTCTGCGTTGATACCACTG5790.10855829336613207No Hit
CAGTGGTATCAACGCAGAGTAAGCA5780.10837080063147553No Hit
GCAGTGGTATCAACGCAGAGTACAT5750.10780832242750593No Hit
GTGGTATCAACGCAGAGTACATGGG5580.10462094593834488No Hit
ACATGGGAAGCAGTGGTATCAACGC5420.10162106218384037No Hit
ATACCACTGCTTCCCATGTACTCTG5370.10068359851055772No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAAAC456.544752E-414.8386413
TAGGACC2900.014.8006944
AATCCCG2000.014.21076719
GATATAC3350.013.6602661
TCCTAGA706.9399593E-613.6273232
TATACAC4000.013.1162983
ACTGTTC3500.013.028128
TCCAACG802.0550215E-613.02653518
AAATCCC2300.012.76909418
GGCGAGG3300.012.63179219
CTGTAGG8650.012.564681
TTGTAGA1301.8553692E-1012.4671811
CTGTTCT3900.012.42264659
GTATAGG1001.3706995E-712.3938441
TGTAGGA8700.012.3898992
TAGAACA3400.012.3435864
GAACATA1404.9112714E-1112.2622766
CAAATCC2400.012.23704917
TGTAGAA2900.012.17060852
CACTGTT3750.012.1618657
Produced by FastQC (version 0.11.5)