##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926531_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 533354 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.803282622798367 31.0 28.0 31.0 24.0 34.0 2 29.185953419305 31.0 29.0 33.0 25.0 34.0 3 29.54474139127109 31.0 30.0 33.0 25.0 34.0 4 33.27859920428084 35.0 33.0 37.0 28.0 37.0 5 33.055102239788205 35.0 33.0 37.0 27.0 37.0 6 33.165826074239625 35.0 35.0 37.0 28.0 37.0 7 33.03205563284423 35.0 35.0 37.0 26.0 37.0 8 33.01031397533345 35.0 35.0 37.0 26.0 37.0 9 34.28204344581648 38.0 35.0 39.0 26.0 39.0 10 34.21510853954409 38.0 34.0 39.0 26.0 39.0 11 34.42828590392122 38.0 35.0 39.0 27.0 39.0 12 34.258259992425295 38.0 34.0 39.0 27.0 39.0 13 34.40418933766316 38.0 35.0 39.0 27.0 39.0 14 35.045376991641575 39.0 34.0 40.0 25.0 41.0 15 35.18696213021745 39.0 35.0 40.0 27.0 41.0 16 35.16787911968411 39.0 35.0 40.0 27.0 41.0 17 35.137985278070474 39.0 35.0 40.0 26.0 41.0 18 35.135587245994216 39.0 35.0 41.0 26.0 41.0 19 35.165824199312276 39.0 35.0 41.0 26.0 41.0 20 35.171531478155224 39.0 35.0 41.0 26.0 41.0 21 35.07874882348309 39.0 35.0 41.0 25.0 41.0 22 35.03220937688665 39.0 35.0 41.0 25.0 41.0 23 34.70807006228509 39.0 34.0 41.0 23.0 41.0 24 34.76948705737653 39.0 35.0 41.0 24.0 41.0 25 34.50763657908256 39.0 34.0 40.0 23.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8140283545002163 1101 2 0.9529215378047766 1101 3 0.8172564892794476 1101 4 0.6696383377306034 1101 5 0.8243734339283364 1101 6 0.2886088675932754 1101 7 0.620374064898229 1101 8 0.6233036488625174 1101 9 1.02475346413582 1101 10 0.9156943192833538 1101 11 0.7769973313715113 1101 12 0.9410035019769296 1101 13 0.7793879098280527 1101 14 0.9733377010801618 1101 15 0.9470369568046593 1101 16 0.8568050913616361 1101 17 0.9177625342345692 1101 18 0.9664059563416032 1101 19 1.1449321702836315 1101 20 1.0290470258680458 1101 21 1.132536343427482 1101 22 1.1242650719565717 1101 23 1.4253852601787855 1101 24 1.3561784292516705 1101 25 1.62899861382283 1102 1 0.9023895615376745 1102 2 0.8808753447766229 1102 3 0.8466615372658062 1102 4 0.8501444810741319 1102 5 0.827365408573641 1102 6 0.33806513039553465 1102 7 0.7614733170784547 1102 8 0.709709233572724 1102 9 1.0368766996416667 1102 10 1.1375629761174224 1102 11 0.8967667371845849 1102 12 1.102749813594393 1102 13 0.9471912022753202 1102 14 1.4288504810199 1102 15 1.1203616654609831 1102 16 0.8296605433978854 1102 17 1.0948002590818717 1102 18 1.15296560282259 1102 19 1.389389598112821 1102 20 1.1969685598549589 1102 21 1.258329112377723 1102 22 1.2508878501458796 1102 23 1.3244916091698542 1102 24 1.2383658049966186 1102 25 1.1784090870092427 1103 1 0.8325392615905542 1103 2 0.46227072188339235 1103 3 0.570849978209349 1103 4 0.24166937942031552 1103 5 0.5902205891193262 1103 6 0.04855507441755691 1103 7 0.45229134915790326 1103 8 0.3515875650522844 1103 9 0.8743910588123924 1103 10 0.9114362750495246 1103 11 0.6173922804018943 1103 12 0.5660091782048866 1103 13 0.3508647187982419 1103 14 0.7919755228058492 1103 15 0.6940064332526816 1103 16 0.7653776510202519 1103 17 0.7575636624940358 1103 18 0.7442413049784378 1103 19 0.8016536974509307 1103 20 0.7378920444686088 1103 21 0.7172658347792407 1103 22 0.5779779072178854 1103 23 0.8547956893016249 1103 24 0.7221443485968351 1103 25 0.8850820747500023 1104 1 -0.05517410527676603 1104 2 0.05765528289836652 1104 3 0.02354702965299893 1104 4 -0.37308871644604835 1104 5 -0.23716929649601326 1104 6 -0.4268092248438293 1104 7 -0.14484844516173467 1104 8 -0.09522061975376062 1104 9 0.30245123150849906 1104 10 0.03540286671628934 1104 11 -0.46405879099722824 1104 12 -0.23739997963653536 1104 13 -0.46283316903174665 1104 14 -0.08373393307182653 1104 15 -0.30962419026744925 1104 16 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0.9396454482921826 1107 15 0.592751990665846 1107 16 0.6602380901305125 1107 17 0.4396704174403965 1107 18 0.7255353848508932 1107 19 0.7333745978151995 1107 20 0.45631673916186344 1107 21 0.5731942662277731 1107 22 0.42361614501945155 1107 23 0.24700109458733266 1107 24 0.6451937623414565 1107 25 0.6829241677758375 1108 1 1.1903786315604243 1108 2 0.9653016184780263 1108 3 1.1288495058019734 1108 4 0.8520992125222477 1108 5 -0.3366588144951663 1108 6 0.38924605662163003 1108 7 0.8460624850672076 1108 8 0.831452064790156 1108 9 1.3185438653154833 1108 10 1.3023787277655785 1108 11 1.083861150484104 1108 12 1.0614343160383797 1108 13 1.0498432818732724 1108 14 1.4201413723613854 1108 15 1.1366740370526784 1108 16 1.28033856219038 1108 17 1.2001068272777928 1108 18 1.1562957585701739 1108 19 1.3234483845962686 1108 20 1.3834159845765726 1108 21 1.5391198608999161 1108 22 1.2553651487620812 1108 23 1.5746846159574943 1108 24 1.4482252685237853 1108 25 1.4581398755208355 1109 1 0.401570275862035 1109 2 -0.43032052890910677 1109 3 0.18755136395339278 1109 4 0.3519453637385084 1109 5 -0.4916871055770713 1109 6 0.21676447573990032 1109 7 0.47209028041413603 1109 8 0.38754704077265245 1109 9 0.9514777616213976 1109 10 -1.3183669260062771 1109 11 -0.029422837276371183 1109 12 0.3722857835848288 1109 13 0.5270651147251115 1109 14 0.772395257043172 1109 15 0.665785645798131 1109 16 0.8596134215896853 1109 17 0.8863835157681947 1109 18 0.8850347113727892 1109 19 0.6889930947622531 1109 20 0.8797748966947267 1109 21 0.6299846620076224 1109 22 0.6665516647708998 1109 23 0.8912028470383717 1109 24 0.9111049758692147 1109 25 0.9406011141744841 1110 1 0.6010670943853 1110 2 -0.3449702350643804 1110 3 0.3362909247328574 1110 4 0.767636230249046 1110 5 1.0344468478496438 1110 6 0.7819322765441115 1110 7 1.0075256345743568 1110 8 0.8821423967003597 1110 9 1.4902906731144867 1110 10 0.8895309795215383 1110 11 0.8021715465334509 1110 12 1.2325355516021546 1110 13 1.1490769568885142 1110 14 1.4428003429872334 1110 15 1.3674310657972413 1110 16 1.015174377800193 1110 17 1.2224104257589161 1110 18 1.2270738391179137 1110 19 1.446005047393598 1110 20 1.124350920732553 1110 21 1.2929994426182887 1110 22 1.3363850791140592 1110 23 1.5495614038851713 1110 24 1.4072910701646464 1110 25 1.3266644785861317 1111 1 0.13027785702024275 1111 2 0.0807035159253644 1111 3 0.29227140019682807 1111 4 0.29680716167176513 1111 5 0.4706336248527947 1111 6 0.006552552320940208 1111 7 0.408110549890786 1111 8 0.3818708202533969 1111 9 0.7879939812777081 1111 10 0.4179716720535467 1111 11 0.3043163969612124 1111 12 0.08100434324387606 1111 13 0.012168631440957256 1111 14 0.35753137367416343 1111 15 0.34295659639727205 1111 16 0.16128009225143813 1111 17 0.42272762704896394 1111 18 0.2883016543397119 1111 19 0.41223180044973873 1111 20 0.17741092343624842 1111 21 0.3568537038337922 1111 22 0.23831822061383434 1111 23 0.7229273585789784 1111 24 0.3694477975356918 1111 25 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0.8820902985038828 2203 1 -0.2125847265888119 2203 2 -0.14936360935135085 2203 3 -0.380042993652399 2203 4 -0.7102707464951408 2203 5 -0.6128052363978327 2203 6 -0.5451684255181988 2203 7 -0.30703667345547814 2203 8 0.021946038648579247 2203 9 0.2783227047072927 2203 10 -0.0035964886662682716 2203 11 -0.16220427619582267 2203 12 -0.008518638832249792 2203 13 -0.08277912033833701 2203 14 0.45785628857740335 2203 15 0.22858178979900146 2203 16 0.012037036861187289 2203 17 0.13876628359130194 2203 18 0.3814891528520121 2203 19 0.4099859600746001 2203 20 0.17814644508533917 2203 21 0.07040729694035974 2203 22 0.06885546086044059 2203 23 0.26412371191501194 2203 24 0.1706796177732457 2203 25 0.19248281996393501 2204 1 0.616075790495767 2204 2 0.6915679224142721 2204 3 0.43422230451571764 2204 4 0.33858595091943755 2204 5 0.2104310894434036 2204 6 0.1737935077025341 2204 7 0.33819523508031324 2204 8 0.4003791944235573 2204 9 0.8741132724609599 2204 10 0.8545950145167893 2204 11 0.5046789250514223 2204 12 0.704285050917008 2204 13 0.5825385358913664 2204 14 1.066502514379664 2204 15 0.85893127661204 2204 16 0.8557878488658659 2204 17 0.7024014661299489 2204 18 0.6779553084970686 2204 19 0.7137991340384815 2204 20 0.7940357526077477 2204 21 0.6289236019942805 2204 22 0.8716227728001513 2204 23 1.1018526972339657 2204 24 0.9359842819281141 2204 25 1.1212992419465238 2205 1 0.6871123615487704 2205 2 0.6122300978989941 2205 3 0.5998671099239132 2205 4 0.5081824487364415 2205 5 0.5202463563322866 2205 6 0.4300514298658129 2205 7 0.4019187691866932 2205 8 0.5372577447263609 2205 9 0.9147991557528243 2205 10 0.8554517818047103 2205 11 0.7764560716642563 2205 12 0.6957807553440247 2205 13 0.7515622906446637 2205 14 1.0113055752688211 2205 15 0.922518666875412 2205 16 1.0732447757707035 2205 17 0.9597062874998414 2205 18 0.809611099305009 2205 19 1.1093850220249593 2205 20 1.0462234433264967 2205 21 0.9070889463443095 2205 22 0.9652498325626055 2205 23 1.2055884373400616 2205 24 1.0691846011619504 2205 25 1.3640237619221125 2206 1 0.2260409993895749 2206 2 0.4144599993432365 2206 3 0.47900569897386447 2206 4 0.2818617307759155 2206 5 0.6325926120105336 2206 6 0.46247609625502406 2206 7 0.5065700606832664 2206 8 0.46945749501636413 2206 9 0.6401380795204332 2206 10 0.5953571222338212 2206 11 0.5104009140901624 2206 12 0.5068623007124344 2206 13 0.529651345022998 2206 14 0.7773419160432766 2206 15 0.4549400126529193 2206 16 0.6418419722889297 2206 17 0.4614927765949517 2206 18 0.49643756856499976 2206 19 0.6317604105365291 2206 20 0.7434832745508757 2206 21 0.6530842886329609 2206 22 0.533116494506622 2206 23 0.9269658713484432 2206 24 0.7028591457954008 2206 25 0.8492304367943788 2207 1 -1.2175838688928273 2207 2 -0.28100891530375804 2207 3 0.05013320160821522 2207 4 0.13749924394767277 2207 5 0.07410999873339819 2207 6 0.38133805621287564 2207 7 0.17780293739559738 2207 8 0.16808763200266696 2207 9 0.6432993018597415 2207 10 0.20968799683867445 2207 11 0.15002156740944628 2207 12 0.05480750619189223 2207 13 -0.09100197426045753 2207 14 -5.700002657191707 2207 15 -2.2847860147443413 2207 16 -0.9756501246552176 2207 17 -0.9431436828360305 2207 18 -0.260148311308555 2207 19 -0.1279866916763268 2207 20 0.14811973398185074 2207 21 0.1114615278321196 2207 22 0.18327455562359063 2207 23 0.48755596618511277 2207 24 0.37198454094819056 2207 25 0.3529185295235635 2208 1 0.5896231634403755 2208 2 0.9596044727501472 2208 3 0.8664356806720512 2208 4 0.6795795500871051 2208 5 0.7202472068749302 2208 6 0.6534804775982721 2208 7 0.7209785793455765 2208 8 0.6476922019100471 2208 9 1.0188885647957946 2208 10 0.8452877971825217 2208 11 0.8339825235405485 2208 12 0.6779703923324263 2208 13 0.5128192225672308 2208 14 0.9792996554920066 2208 15 0.8291843695937473 2208 16 0.8341912596274028 2208 17 0.7306126257436389 2208 18 0.8910080305471411 2208 19 0.5984427908814922 2208 20 0.5711273320074781 2208 21 1.0362438471710362 2208 22 1.017005352584377 2208 23 1.0365133174135508 2208 24 1.005130234476006 2208 25 0.9464796187587723 2209 1 -0.2704828076664576 2209 2 -0.5029981245375232 2209 3 -0.033213663369011215 2209 4 0.016816558117895397 2209 5 -0.029548151634834596 2209 6 0.017857371914764997 2209 7 0.2797405536381774 2209 8 0.23282416801698957 2209 9 0.4254824454621513 2209 10 0.2085651583028536 2209 11 0.21179496131847486 2209 12 0.04277758686352229 2209 13 0.26851710535743223 2209 14 0.5059627824139739 2209 15 0.4496263952126469 2209 16 0.400440900122085 2209 17 0.34703925347311326 2209 18 0.44732979333999623 2209 19 0.4438720955428934 2209 20 -0.003363276607380783 2209 21 0.27690113351425794 2209 22 0.44812855782320327 2209 23 0.6142317307229916 2209 24 0.2780058345733494 2209 25 0.20375490027909393 2210 1 -0.7344701571063013 2210 2 -0.5332242915580743 2210 3 0.19214229304266794 2210 4 0.38373891402967786 2210 5 0.6278218836260478 2210 6 0.7673699045135507 2210 7 0.8227033607362628 2210 8 0.6650167829974478 2210 9 1.0501280831230702 2210 10 0.9049134567897568 2210 11 0.6005701396952006 2210 12 0.5231073686030996 2210 13 0.5542144483572216 2210 14 0.6484142110218443 2210 15 0.5380170832781417 2210 16 0.6426264374201907 2210 17 0.6367039243608872 2210 18 0.5477831475875661 2210 19 0.7082777296330889 2210 20 0.8410348334601494 2210 21 0.6251792162912579 2210 22 0.6595395671262096 2210 23 0.8647804105120613 2210 24 0.7198763831256088 2210 25 0.6918369758891458 2211 1 -1.6286169469344003 2211 2 -0.7106667144572363 2211 3 -0.15683914799824805 2211 4 0.07713343695295549 2211 5 0.15679980452720343 2211 6 -0.19939084983936084 2211 7 0.02087679932612474 2211 8 -0.027553766470298058 2211 9 0.1713199763715707 2211 10 0.29146652289668395 2211 11 -0.03945515316087267 2211 12 0.12551410119633744 2211 13 -0.18729386634327483 2211 14 0.13130698365340976 2211 15 0.019825131360647674 2211 16 0.012212429871446773 2211 17 -0.24843652269686345 2211 18 -0.21701675854203728 2211 19 -0.26832377098945415 2211 20 -0.4439530742078901 2211 21 0.07473365786020025 2211 22 -0.09485767412721913 2211 23 0.04710409388201242 2211 24 0.04324685176051446 2211 25 0.19727689557780792 2212 1 0.9572276540425797 2212 2 0.3498331101183716 2212 3 0.5449204935962229 2212 4 0.5719840563359995 2212 5 0.6395933705002719 2212 6 0.8131452471028524 2212 7 1.0073718199722208 2212 8 0.8893261083036776 2212 9 1.108431681226783 2212 10 1.031820880168695 2212 11 0.9059622219504533 2212 12 1.045149488344471 2212 13 0.9143672677079522 2212 14 1.08098852091085 2212 15 0.8585952714302749 2212 16 0.4787374076060473 2212 17 1.0998942467415063 2212 18 1.137707555278702 2212 19 0.794588813686552 2212 20 0.9990205041798248 2212 21 1.160610644878794 2212 22 1.3192262057205681 2212 23 1.4035799527868207 2212 24 1.5491707985394498 2212 25 0.8975103449611197 2213 1 0.41503118865407984 2213 2 0.4196456172982401 2213 3 -0.30322604223056615 2213 4 -0.9244789136808649 2213 5 -1.8046016679838246 2213 6 -0.5532759436367769 2213 7 -0.2876708964668069 2213 8 0.009768025434389926 2213 9 -0.47734280741542534 2213 10 -0.19376152053749962 2213 11 -0.08061973738276862 2213 12 -0.4499818049992612 2213 13 -0.30384178587956256 2213 14 -7.213484340697562 2213 15 -3.173024869688149 2213 16 -1.9185753565375379 2213 17 -1.1254447942094359 2213 18 -4.916653874769729 2213 19 -2.7461263314526008 2213 20 -5.170699496062024 2213 21 -2.3063921907913993 2213 22 -1.5689446963127764 2213 23 -4.5454172840974465 2213 24 -2.296004027480997 2213 25 -1.5189326121168634 2214 1 0.6081363266903814 2214 2 0.35472153891984703 2214 3 -1.512877988115303 2214 4 -1.651675939063125 2214 5 -0.07814299941001224 2214 6 -0.17218946649272482 2214 7 -0.275524430315329 2214 8 -0.3102368086505436 2214 9 -5.1266729886604985 2214 10 -1.285926991926999 2214 11 -0.8709528919211778 2214 12 -0.968940688789182 2214 13 -1.0175397946338975 2214 14 -2.3326875775478584 2214 15 -1.6720580527013098 2214 16 -1.4662821461091582 2214 17 -1.552509710010277 2214 18 -0.8758075125841884 2214 19 -3.476632718404545 2214 20 -1.5031074001219906 2214 21 -3.2864217912840736 2214 22 -5.484719043876858 2214 23 -5.540404404241013 2214 24 -4.907005875028524 2214 25 -2.7023861353701406 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14712.0 3 5.0 4 246.0 5 1688.0 6 2073.0 7 1716.0 8 1352.0 9 1103.0 10 878.0 11 917.0 12 860.0 13 835.0 14 924.0 15 865.0 16 905.0 17 935.0 18 1042.0 19 1399.0 20 1669.0 21 2245.0 22 2903.0 23 3524.0 24 4341.0 25 4856.0 26 5864.0 27 6239.0 28 7471.0 29 9157.0 30 11359.0 31 14278.0 32 18543.0 33 24566.0 34 32810.0 35 44685.0 36 61729.0 37 94393.0 38 150267.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.36804102625688 19.21576501096773 9.708646775261903 27.707547187513487 2 11.99676670380081 22.52504679277272 39.34325077550347 26.134935727923004 3 22.471501288490607 24.259071011399485 28.40287181487747 24.866555885232437 4 11.901988916082933 17.4345701934528 37.75058373421105 32.912857156253224 5 12.296267124251035 39.7644565716804 34.89455868425741 13.044717619811152 6 31.61976416390345 36.85362429570971 17.157395941932982 14.369215598453856 7 27.394562578453257 31.83999669274885 21.44489292774408 19.320547801053813 8 24.353618776484296 37.05499337599008 19.32669992765266 19.26468791987297 9 24.985341872632148 17.749676769499068 21.233800890011427 36.03118046785736 10 18.09438264613446 29.18019937143243 31.431753913545705 21.293664068887406 11 31.831860547527572 22.652401925215106 24.58774184717902 20.927995680078297 12 27.486082960368545 25.10073330470864 29.73305908624721 17.68012464867561 13 30.584939833581448 21.2463017058089 24.760853016945596 23.40790544366406 14 20.443322025757258 24.00483323826964 28.811241031491097 26.740603704482 15 25.39542959140817 31.445183596328075 22.26105477890442 20.89833203335933 16 19.139278100584427 28.818040173021863 31.844797813039637 20.19788391335407 17 21.981201238250776 29.293330808331756 28.354517721573576 20.370950231843896 18 20.85507553442221 29.334768334230212 30.627956642472903 19.18219948887468 19 22.861042779144416 28.860360292794144 26.86521269574608 21.413384232315355 20 22.741479749581416 27.902285003946776 26.471134177186713 22.8851010692851 21 22.111955661718106 27.67730250452795 27.196196147399288 23.014545686354655 22 21.770156406439252 28.099536142974458 24.935221260176167 25.19508619041012 23 20.181905451163768 30.663499289402534 28.385837548794985 20.768757710638713 24 26.07935082393837 27.498111007156613 26.711390017493102 19.711148151411916 25 21.098557430899554 28.40064947483285 27.271568226731212 23.229224867536384 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 287.0 1 287.0 2 302.0 3 317.0 4 317.0 5 317.0 6 425.0 7 533.0 8 533.0 9 533.0 10 797.0 11 1061.0 12 1061.0 13 1061.0 14 1447.5 15 1834.0 16 1834.0 17 1834.0 18 3104.5 19 4375.0 20 4375.0 21 4375.0 22 7438.0 23 10501.0 24 10501.0 25 10501.0 26 16014.5 27 21528.0 28 21528.0 29 21528.0 30 30162.0 31 38796.0 32 38796.0 33 38796.0 34 43278.0 35 47760.0 36 47760.0 37 47760.0 38 50897.0 39 54034.0 40 54034.0 41 54034.0 42 59817.0 43 65600.0 44 65600.0 45 65600.0 46 84665.0 47 103730.0 48 103730.0 49 103730.0 50 91919.0 51 80108.0 52 80108.0 53 80108.0 54 61516.5 55 42925.0 56 42925.0 57 42925.0 58 34953.0 59 26981.0 60 26981.0 61 26981.0 62 21963.5 63 16946.0 64 16946.0 65 16946.0 66 13108.0 67 9270.0 68 9270.0 69 9270.0 70 6714.0 71 4158.0 72 4158.0 73 4158.0 74 2911.0 75 1664.0 76 1664.0 77 1664.0 78 1153.5 79 643.0 80 643.0 81 643.0 82 431.0 83 219.0 84 219.0 85 219.0 86 139.5 87 60.0 88 60.0 89 60.0 90 39.5 91 19.0 92 19.0 93 19.0 94 12.0 95 5.0 96 5.0 97 5.0 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0800593976983392 2 0.028498895667792873 3 0.03224875036092351 4 0.02793641746382328 5 0.022311635424127316 6 0.3689857018040551 7 0.22311635424127316 8 0.22480378885318195 9 0.22986609268890829 10 0.0146244333032095 11 0.0020624200812218527 12 0.002812391019847981 13 0.0 14 0.07087225370016913 15 0.0018749273465653205 16 0.0020624200812218527 17 0.003937347427787174 18 0.003937347427787174 19 0.0018749273465653205 20 0.0013124491425957245 21 0.0 22 0.0 23 0.0 24 1.8749273465653205E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 533354.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.38041290381447 #Duplication Level Percentage of deduplicated Percentage of total 1 92.30538008776279 63.11880003644754 2 5.298577988868395 7.246379013637676 3 1.0070588221337924 2.0658929422781336 4 0.402788334751966 1.1017133057271713 5 0.20974979896198204 0.7171388929756205 6 0.13998292685479882 0.5743254202689363 7 0.09630457738469983 0.46097427362651905 8 0.07759917202474571 0.42450107392369935 9 0.05599984170042751 0.3446363068221133 >10 0.3226921412011846 4.27711144746366 >50 0.03657121081481435 1.732853623956222 >100 0.03409646566868878 5.296898623078007 >500 0.007149258930531492 3.4145638775725544 >1k 0.005499429946562686 7.000796082249941 >5k 5.499429946562686E-4 2.223415079972217 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGA 6663 1.2492640910164732 No Hit CCCATGTACTCTGCGTTGATACCAC 5162 0.9678374962970184 No Hit GTACATGGGAAGCAGTGGTATCAAC 4897 0.9181519216130375 No Hit GCTTACTCTGCGTTGATACCACTGC 4286 0.8035938607378964 No Hit CTGCTTACTCTGCGTTGATACCACT 2833 0.5311669172819553 No Hit GAGTACATGGGAAGCAGTGGTATCA 2347 0.44004544823888075 No Hit GCGTTGATACCACTGCTTACTCTGC 2037 0.3819227004953558 No Hit CATGTACTCTGCGTTGATACCACTG 1958 0.36711077445748974 No Hit CTGTAGGACGTGGAATATGGCAAGA 1777 0.33317458948465745 No Hit GTCCTACAGTGGACATTTCTAAATT 1720 0.3224875036092351 No Hit GCGTTGATACCACTGCTTCCCATGT 1645 0.30842554850999526 No Hit GTATCAACGCAGAGTAAGCAGTGGT 1537 0.28817633316708974 No Hit ACGCAGAGTACATGGGAAGCAGTGG 1413 0.2649272340696798 No Hit GTATCAACGCAGAGTACATGGGAAG 1368 0.25649006101013583 No Hit ACGCAGAGTAAGCAGTGGTATCAAC 1364 0.2557400900715097 No Hit GTATCAACGCAGAGTACTTTTTTTT 1359 0.25480262639822704 No Hit CTTTAGGACGTGAAATATGGCGAGG 1204 0.2257412525264646 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1167 0.21880402134417293 No Hit GCTTCCCATGTACTCTGCGTTGATA 1132 0.21224177563119428 No Hit ACTCTGCGTTGATACCACTGCTTCC 1103 0.20680448632615483 No Hit GTCCTACAGTGTGCATTTCTCATTT 1055 0.19780483506264135 No Hit TATCAACGCAGAGTAAGCAGTGGTA 1031 0.19330500943088455 No Hit GTGGTATCAACGCAGAGTAAGCAGT 990 0.18561780730996674 No Hit ACTCTGCGTTGATACCACTGCTTAC 952 0.17849308339301853 No Hit TATCAACGCAGAGTACATGGGAAGC 909 0.17043089580278764 No Hit GGTATCAACGCAGAGTAAGCAGTGG 908 0.1702434030681311 No Hit GTAAGCAGTGGTATCAACGCAGAGT 883 0.16555608470171781 No Hit GGTATCAACGCAGAGTACATGGGAA 852 0.1597438099273653 No Hit CTGTAGGACCTGGAATATGGCGAGA 837 0.15693141890751733 No Hit GGTATCAACGCAGAGTACTTTTTTT 822 0.15411902788766935 No Hit CTGAAGGACCTGGAATATGGCGAGA 762 0.14286946380827742 No Hit GATATACACTGTTCTACAAATCCCG 759 0.14230698560430782 No Hit AAGCAGTGGTATCAACGCAGAGTAA 706 0.13236987066751163 No Hit CCACTGCTTACTCTGCGTTGATACC 704 0.13199488519819857 No Hit TATCAACGCAGAGTACTTTTTTTTT 698 0.13086992879025938 No Hit CAGTGGTATCAACGCAGAGTACATG 670 0.12562013221987647 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 611 0.11455806087514109 No Hit CATGGGAAGCAGTGGTATCAACGCA 606 0.11362059720185842 No Hit GTGTATATCAATGAGTTACAATGAA 580 0.10874578610078858 No Hit TGCTTACTCTGCGTTGATACCACTG 579 0.10855829336613207 No Hit CAGTGGTATCAACGCAGAGTAAGCA 578 0.10837080063147553 No Hit GCAGTGGTATCAACGCAGAGTACAT 575 0.10780832242750593 No Hit GTGGTATCAACGCAGAGTACATGGG 558 0.10462094593834488 No Hit ACATGGGAAGCAGTGGTATCAACGC 542 0.10162106218384037 No Hit ATACCACTGCTTCCCATGTACTCTG 537 0.10068359851055772 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTAAAC 45 6.544752E-4 14.838641 3 TAGGACC 290 0.0 14.800694 4 AATCCCG 200 0.0 14.210767 19 GATATAC 335 0.0 13.660266 1 TCCTAGA 70 6.9399593E-6 13.627323 2 TATACAC 400 0.0 13.116298 3 ACTGTTC 350 0.0 13.02812 8 TCCAACG 80 2.0550215E-6 13.026535 18 AAATCCC 230 0.0 12.769094 18 GGCGAGG 330 0.0 12.631792 19 CTGTAGG 865 0.0 12.56468 1 TTGTAGA 130 1.8553692E-10 12.467181 1 CTGTTCT 390 0.0 12.4226465 9 GTATAGG 100 1.3706995E-7 12.393844 1 TGTAGGA 870 0.0 12.389899 2 TAGAACA 340 0.0 12.343586 4 GAACATA 140 4.9112714E-11 12.262276 6 CAAATCC 240 0.0 12.237049 17 TGTAGAA 290 0.0 12.1706085 2 CACTGTT 375 0.0 12.161865 7 >>END_MODULE