FastQCFastQC Report
Thu 26 May 2016
SRR1926530_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926530_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences593258
Sequences flagged as poor quality0
Sequence length25
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC19300.32532220383037397No Hit
CCCATGTACTCTGCGTTGATACCAC17170.28941876889987156No Hit
GAGTACATGGGAAGCAGTGGTATCA9470.15962700882246847No Hit
CATGTACTCTGCGTTGATACCACTG6640.11192432297583851No Hit
GTCCTACAGTGGACATTTCTAAATT6510.10973303351998626No Hit
GCGTTGATACCACTGCTTCCCATGT6440.1085531084283735No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT300.002410641622.2537041
CTAACCT557.10116E-515.5638774
GTATTAA952.962861E-715.4604671
TTGTAGA1403.8198777E-1115.2596831
GACCGTG400.003353493515.2534427
GTCCAAG801.5540381E-515.021251
TGTAGAA1651.8189894E-1214.9129252
TATAGGA751.2366736E-414.5815262
CCTACAC1207.8216544E-1114.571363
CTAAAAG450.00454519314.571363
GATATAC2000.014.0198331
GAACAAT450.00632450813.8636366
TCCTACA4250.013.8310072
GTCCTAC3600.013.7231181
TAGGACC1451.0913936E-1113.2829664
TATACTG550.00155008113.2380525
ATCCCGT701.004238E-513.14751110
TGTAGGA2600.013.1444052
TCTACAC1003.5246376E-713.11422353
CCAACGA651.03716175E-513.10688119
Produced by FastQC (version 0.11.5)