FastQCFastQC Report
Thu 26 May 2016
SRR1926530_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926530_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences593258
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC48770.8220706673993441No Hit
GTACATGGGAAGCAGTGGTATCAAC47040.7929096615637716No Hit
GAGTACATGGGAAGCAGTGGTATCA21940.36982223585691215No Hit
CATGTACTCTGCGTTGATACCACTG17130.28874452599037853No Hit
GCGTTGATACCACTGCTTCCCATGT15020.25317821251462264No Hit
GTATCAACGCAGAGTACTTTTTTTT13890.23413085032144532No Hit
ACGCAGAGTACATGGGAAGCAGTGG13870.2337937288666988No Hit
GTATCAACGCAGAGTACATGGGAAG13360.22519713177066303No Hit
GTCCTACAGTGGACATTTCTAAATT12430.20952098412495068No Hit
CTGTAGGACGTGGAATATGGCAAGA12180.20530696594061945No Hit
ACTCTGCGTTGATACCACTGCTTCC11000.18541680011057582No Hit
GCTTCCCATGTACTCTGCGTTGATA10080.16990921319223676No Hit
GGTATCAACGCAGAGTACTTTTTTT9130.15389594409177795No Hit
TATCAACGCAGAGTACATGGGAAGC8600.14496222554099564No Hit
GGTATCAACGCAGAGTACATGGGAA7740.1304660029868961No Hit
CTTTAGGACGTGAAATATGGCGAGG7610.12827471353104383No Hit
GTCCTAAAGTGTGTATTTCTCATTT7380.12439781680145907No Hit
CAGTGGTATCAACGCAGAGTACATG7330.1235550131645928No Hit
GATATACACTGTTCTACAAATCCCG7190.1211951629813673No Hit
TATCAACGCAGAGTACTTTTTTTTT6930.11681258406966279No Hit
GTCCTACAGTGTGCATTTCTCATTT6870.11580121970542327No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6480.10922735133786649No Hit
GTGGTATCAACGCAGAGTACATGGG6180.10417052951666897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC1750.015.796174
CCGTTTC551.145999E-515.51654513
TCCAACG755.996117E-815.1640618
GTCTTAC400.00516569814.2946211
CCCGTTT602.6083586E-514.22349912
TAGACTG551.9015826E-413.8649785
GTAGGAC5250.013.6185443
TCCCGTT655.537257E-513.12938411
TGTAGGA5400.013.0659282
AGGACCT4050.012.9449255
GTTCAAG751.4282465E-512.7063311
GGACCTG4200.012.4815486
GTGTAAA1001.3791941E-712.3886741
GATATAC2850.012.3719541
TTAGACT550.00298793912.1318564
TAGGACT550.00298793912.1318564
CTGTAGG5650.011.9754361
CAGAGGT1209.471478E-911.91521554
AATGCCG1302.6975613E-911.66466118
CCAACGA658.177031E-411.66367819
Produced by FastQC (version 0.11.5)