FastQCFastQC Report
Thu 26 May 2016
SRR1926529_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926529_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478893
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC20090.4195091596661467No Hit
CCCATGTACTCTGCGTTGATACCAC18610.38860455258272725No Hit
GAGTACATGGGAAGCAGTGGTATCA9710.20275928025675882No Hit
GTCCTACAGTGGACATTTCTAAATT8190.1710194135224361No Hit
CATGTACTCTGCGTTGATACCACTG7270.15180844155166184No Hit
GCGTTGATACCACTGCTTCCCATGT7020.14658806873351668No Hit
ACGCAGAGTACATGGGAAGCAGTGG6470.1351032485335973No Hit
CTGTAGGACGTGGAATATGGCAAGA5980.12487131781003273No Hit
GTATCAACGCAGAGTACATGGGAAG5940.12403605815912949No Hit
GTCCTACAGTGTGCATTTCTCATTT4940.10315456688654878No Hit
GCTTCCCATGTACTCTGCGTTGATA4920.10273693706109716No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTATA200.0082096827.2575282
CTGGACT303.4336533E-421.4206354
CCCCTAC350.00598059919.021431
GTTCTAG350.00598059919.021431
GAACTAA350.00598059919.021431
CCCCTGT552.3767732E-416.9463651
GTAGCAC400.002097393416.3636973
TCTACAC802.3404937E-816.3636973
TGTAGAA2350.015.658582
CTGTACT557.037521E-515.5786444
GTCTTAC604.6120226E-415.5341671
GTCCTAC4900.015.2171441
TCCTATA550.003971042614.8677422
CTGTAGG4450.014.3622481
GTCCTAA1950.014.33923151
CTACACC450.00519388114.2804244
TAGGACC2100.014.2804244
TGTAGGA4500.014.2344872
GCAATAT459.749308E-414.07649412
CCTGTAC702.9680421E-514.0260273
Produced by FastQC (version 0.11.5)