##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926529_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 478893 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.306772076434612 27.0 7.0 31.0 2.0 31.0 2 16.474989193828268 16.0 2.0 30.0 2.0 31.0 3 20.118575548191348 25.0 15.0 31.0 2.0 31.0 4 22.22228556274575 28.0 2.0 35.0 2.0 37.0 5 22.866076555723303 30.0 2.0 35.0 2.0 37.0 6 23.610743944889567 31.0 2.0 35.0 2.0 37.0 7 23.315475481997023 30.0 7.0 35.0 2.0 37.0 8 23.58376923446365 31.0 8.0 35.0 2.0 37.0 9 26.172572996473114 32.0 17.0 37.0 2.0 39.0 10 26.816693917012778 32.0 23.0 37.0 2.0 39.0 11 24.031597872593668 30.0 7.0 37.0 2.0 39.0 12 25.120170894124573 31.0 10.0 37.0 2.0 39.0 13 21.86141163057301 27.0 2.0 37.0 2.0 39.0 14 25.162027843380464 31.0 17.0 38.0 2.0 40.0 15 26.250078827629554 31.0 17.0 38.0 2.0 40.0 16 26.57783471464398 31.0 17.0 39.0 2.0 40.0 17 25.920291171514304 31.0 11.0 38.0 2.0 40.0 18 25.997118354204385 31.0 15.0 38.0 2.0 40.0 19 26.38781105591437 31.0 17.0 39.0 2.0 40.0 20 26.653530120507085 31.0 17.0 39.0 2.0 40.0 21 24.520293677293257 30.0 2.0 38.0 2.0 40.0 22 25.45948051026012 31.0 2.0 38.0 2.0 40.0 23 25.449666209362007 31.0 2.0 38.0 2.0 40.0 24 24.51662062297841 31.0 2.0 39.0 2.0 40.0 25 24.312222145656754 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.642249958826643 1101 2 10.251754531995857 1101 3 7.039872173652142 1101 4 8.43978927591623 1101 5 8.185912355960788 1101 6 7.809460402328174 1101 7 7.424722139295106 1101 8 5.948541472970803 1101 9 5.559906543007152 1101 10 5.211476030855113 1101 11 8.018926772224336 1101 12 5.405895655867923 1101 13 10.024668933844087 1101 14 7.4401863165096955 1101 15 6.828687713716228 1101 16 6.179197434664658 1101 17 2.6384832905896545 1101 18 5.069110862010945 1101 19 5.6685996439959965 1101 20 3.225631475607255 1101 21 0.689731951348282 1101 22 4.305138817622886 1101 23 5.557199420203727 1101 24 6.772231061429526 1101 25 7.487392543096242 1102 1 4.86163863983289 1102 2 10.093644660499013 1102 3 6.042397011546889 1102 4 7.413920129636946 1102 5 7.2787667622566055 1102 6 6.105762299773378 1102 7 6.104604956858644 1102 8 6.395506914522173 1102 9 4.957969633708316 1102 10 4.3001895877799114 1102 11 6.934423198851366 1102 12 4.584384267451931 1102 13 8.849240950056053 1102 14 6.201209617609219 1102 15 4.5215634363844615 1102 16 4.847302956469289 1102 17 5.425711326804443 1102 18 5.407616296245614 1102 19 3.818135436777812 1102 20 4.196922066733304 1102 21 3.489719564807558 1102 22 4.408029202498632 1102 23 4.649888192524703 1102 24 6.139018136794423 1102 25 6.149219817106687 1103 1 4.961717456155036 1103 2 9.229338602389891 1103 3 6.492056071244285 1103 4 7.681828451924051 1103 5 7.063183222926067 1103 6 6.762627679106782 1103 7 6.345523167011617 1103 8 6.419281678968847 1103 9 5.122828600259147 1103 10 4.426836072691362 1103 11 5.257677596443884 1103 12 4.315063819393082 1103 13 8.519734985093493 1103 14 6.693571300934789 1103 15 5.608856991358845 1103 16 5.3171945243170065 1103 17 2.9145456811674038 1103 18 1.9786286083104834 1103 19 4.071813531811188 1103 20 4.530473339139231 1103 21 6.705795706151495 1103 22 2.6243874704502446 1103 23 4.55221715513472 1103 24 5.928900987079238 1103 25 1.7554770568635476 1104 1 5.236846122722358 1104 2 10.052923556711825 1104 3 6.83574768413272 1104 4 7.021059719332726 1104 5 7.416494739300248 1104 6 6.922520963227782 1104 7 6.735984647278922 1104 8 6.712462350900623 1104 9 5.5883966817804485 1104 10 4.597079994700508 1104 11 7.623732547670947 1104 12 4.80062536728914 1104 13 8.839212065823883 1104 14 6.6254883468191785 1104 15 5.7241625816203054 1104 16 5.220006848797468 1104 17 5.591664237809347 1104 18 3.395324886436594 1104 19 3.887046621757431 1104 20 3.524148904527067 1104 21 2.2058931374051483 1104 22 4.511334261523249 1104 23 4.686450199230958 1104 24 5.912910798979606 1104 25 5.151356001396511 1105 1 3.6052204239919625 1105 2 9.422184734999284 1105 3 6.334737707465699 1105 4 5.597941045675491 1105 5 2.2632706475798194 1105 6 4.915862391295622 1105 7 5.743339851316591 1105 8 3.2407570850333585 1105 9 3.9385983973419236 1105 10 3.5434099465237807 1105 11 6.6436229330288015 1105 12 3.7685159428919093 1105 13 5.193284426035838 1105 14 3.7635627885342906 1105 15 4.424603781953795 1105 16 4.334690958311391 1105 17 5.086933294474356 1105 18 2.238716953223747 1105 19 2.327070514280244 1105 20 1.664095020453363 1105 21 2.4070449584406397 1105 22 3.9216715545619643 1105 23 1.7941246230406662 1105 24 4.308208951925323 1105 25 5.108206776644526 1106 1 6.109034540365322 1106 2 11.02292325398425 1106 3 7.875132541699394 1106 4 8.67965869611352 1106 5 8.234494920936793 1106 6 7.340088244431428 1106 7 7.240147151441427 1106 8 7.094867824215186 1106 9 6.105773244611559 1106 10 5.334948187114154 1106 11 7.576855182611762 1106 12 5.142934573234708 1106 13 4.628952628291717 1106 14 5.613878744300486 1106 15 5.839199060293147 1106 16 5.746919330255405 1106 17 6.0858064592242975 1106 18 6.361116670410194 1106 19 6.095989194336369 1106 20 5.640986612273867 1106 21 6.061567765807048 1106 22 6.130381880570869 1106 23 6.550222790276276 1106 24 7.335230903117893 1106 25 6.303491744441345 1107 1 4.316608119434228 1107 2 -13.832554601493605 1107 3 -5.3621994409596905 1107 4 2.277293926926319 1107 5 4.863881889108811 1107 6 5.060207299830857 1107 7 5.318200328793672 1107 8 5.683674656012673 1107 9 4.274482261451414 1107 10 3.6436834549615718 1107 11 6.359290426495605 1107 12 3.300977075903379 1107 13 4.829269056411885 1107 14 4.363099928101107 1107 15 4.2504276292319965 1107 16 3.7522730108427282 1107 17 4.328788944029213 1107 18 -0.5678394980319545 1107 19 2.1625834256502223 1107 20 2.686130720502085 1107 21 -0.6916727631344131 1107 22 2.169074885385367 1107 23 -0.614521344094026 1107 24 2.76817373909811 1107 25 3.5441759045180277 1108 1 5.046988426356158 1108 2 10.754050479337803 1108 3 0.35853289807896616 1108 4 5.702403464816719 1108 5 7.045094252321345 1108 6 6.967469715923752 1108 7 7.2583295079609655 1108 8 7.123650050456998 1108 9 5.65466507641332 1108 10 4.950295118858314 1108 11 7.440529229888583 1108 12 2.9961244933992255 1108 13 3.169502917109906 1108 14 5.003794063777004 1108 15 5.0042722473062895 1108 16 4.838915863360484 1108 17 0.0823745562542868 1108 18 3.777682398754216 1108 19 4.4412332497420834 1108 20 4.807760946484688 1108 21 4.62980745529385 1108 22 1.4300529562234665 1108 23 4.446393594068326 1108 24 5.288583939611804 1108 25 5.833396404830992 1109 1 4.035070374150234 1109 2 -13.832554601493605 1109 3 -5.482818240605631 1109 4 2.568901679452445 1109 5 2.254166516254145 1109 6 2.8695999381497614 1109 7 4.3764376751858265 1109 8 4.487757837031399 1109 9 4.053910593117909 1109 10 3.2042486283703155 1109 11 1.5494293489128665 1109 12 2.188320306812326 1109 13 3.980852266169215 1109 14 4.094198547498518 1109 15 4.025868479434891 1109 16 4.029344776887495 1109 17 4.469991769608871 1109 18 4.745869678779439 1109 19 3.2584798402218453 1109 20 2.0259503570162067 1109 21 1.187136538977299 1109 22 3.6159012812194185 1109 23 4.02504194406222 1109 24 5.097197718590241 1109 25 5.505923384142356 1110 1 3.3330766794560063 1110 2 9.354338602389891 1110 3 6.581861896487002 1110 4 7.9087701994968675 1110 5 8.377503611275582 1110 6 4.182530591728142 1110 7 6.611299866040746 1110 8 7.350106921687296 1110 9 5.499042192492155 1110 10 5.060331218322428 1110 11 3.4652018682885455 1110 12 5.983753139781431 1110 13 10.334055373443007 1110 14 7.853765475692068 1110 15 7.046963787475349 1110 16 6.438553747617981 1110 17 6.748283545245073 1110 18 6.870618899572619 1110 19 5.887347512393713 1110 20 6.206444212925639 1110 21 4.265261725568969 1110 22 6.280940868508495 1110 23 6.74881909688229 1110 24 7.883998074457878 1110 25 8.237273173368404 1111 1 4.957850027478681 1111 2 9.506688897324363 1111 3 7.34329204177379 1111 4 7.837406385776102 1111 5 8.328503748988176 1111 6 7.536933951915433 1111 7 7.404309230497123 1111 8 7.443699843259971 1111 9 6.06379867072209 1111 10 5.465757094518807 1111 11 7.28440531569381 1111 12 6.447551941681866 1111 13 8.476263709645352 1111 14 6.365651793945876 1111 15 6.056775809784792 1111 16 5.420083045972312 1111 17 6.796158754682978 1111 18 6.605657000056908 1111 19 5.4943674118635215 1111 20 4.788575430075451 1111 21 7.582083892706155 1111 22 7.287269909799324 1111 23 7.271114192018743 1111 24 7.0049642430639985 1111 25 7.456155865190571 1112 1 -1.125879956424935 1112 2 8.46098985648447 1112 3 6.344816734891506 1112 4 0.20663983515312268 1112 5 4.823985819806499 1112 6 3.964698915936129 1112 7 6.348003566240596 1112 8 6.536213729629303 1112 9 5.267985169917271 1112 10 4.111600686056544 1112 11 0.3084563058085017 1112 12 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2204 6 3.6207392481040124 2204 7 4.500053911348189 2204 8 4.428469474363599 2204 9 3.0134219508214137 2204 10 2.3377403589710752 2204 11 4.172988066514431 2204 12 4.143429282362511 2204 13 2.3695405775127583 2204 14 3.354885938545088 2204 15 3.6163451755687603 2204 16 3.585040441757812 2204 17 -1.6337186200130134 2204 18 1.9603519674872203 2204 19 2.5696773766576406 2204 20 3.015950976446863 2204 21 4.364581299036409 2204 22 3.86593888950917 2204 23 2.8328945549624294 2204 24 4.295888950106875 2204 25 4.44774557214841 2205 1 5.504571000760272 2205 2 9.982303889072433 2205 3 6.742136214127594 2205 4 7.328100662951712 2205 5 7.40404235463517 2205 6 6.5560193259285455 2205 7 6.524424980530579 2205 8 6.41573249413646 2205 9 5.164823471114612 2205 10 4.8035648691742345 2205 11 6.988202875799079 2205 12 6.20475286500583 2205 13 9.227247807954821 2205 14 6.62174037952061 2205 15 5.227239478993944 2205 16 4.8435157369933215 2205 17 5.689538811777403 2205 18 5.658366197612551 2205 19 4.388469512760082 2205 20 4.605864894369127 2205 21 -1.9642216963031665 2205 22 2.712464499210089 2205 23 4.185712300765786 2205 24 5.9861688016973 2205 25 6.367322175017058 2206 1 4.40465853811698 2206 2 8.801015138459114 2206 3 6.25700362569814 2206 4 1.9319690335302404 2206 5 5.726848902652478 2206 6 6.289889034657747 2206 7 6.358209939716982 2206 8 6.345591096215056 2206 9 5.048432240462041 2206 10 4.250627070878146 2206 11 3.603554251864022 2206 12 5.009017662722055 2206 13 8.226854726193814 2206 14 1.3089142052933909 2206 15 3.088690703401813 2206 16 3.83143056370281 2206 17 4.7015961069288394 2206 18 -0.4522626216925012 2206 19 1.5203721399959065 2206 20 3.2743910329123267 2206 21 3.822036611382277 2206 22 -1.7387348183330644 2206 23 -2.563401174411517 2206 24 1.639716016113642 2206 25 3.0861795058525097 2207 1 1.2482423369916944 2207 2 -13.832554601493605 2207 3 -6.394788530392969 2207 4 -4.387472202742348 2207 5 0.9159799015773835 2207 6 1.656273667371977 2207 7 2.8171025643437666 2207 8 2.8744461068091525 2207 9 0.45742139541531657 2207 10 0.4984248391312178 2207 11 -1.761400249129796 2207 12 -2.4389429380758543 2207 13 0.7029303192778507 2207 14 -5.476792747456653 2207 15 -1.6485343562701829 2207 16 -0.7042870829534209 2207 17 0.268434446436121 2207 18 0.4652132172087917 2207 19 -0.2320354147497632 2207 20 -0.37731414607514324 2207 21 -4.425430358706155 2207 22 -4.383782684257149 2207 23 -1.7052708114175665 2207 24 -0.3478975888163909 2207 25 0.18210669603578822 2208 1 4.842854137152077 2208 2 6.517388773364832 2208 3 6.397728481056376 2208 4 8.135834268370633 2208 5 3.6303719770629996 2208 6 6.371488139364729 2208 7 6.653885545927274 2208 8 6.750322976839083 2208 9 5.493444274977943 2208 10 4.752878689982371 2208 11 6.939191411878909 2208 12 6.43732327249317 2208 13 9.035084246767617 2208 14 3.6102924853348384 2208 15 5.130653547670626 2208 16 5.261319803319523 2208 17 6.186131954793282 2208 18 0.9798380773535733 2208 19 3.5840319179396793 2208 20 4.504472503506076 2208 21 7.299590030443131 2208 22 6.330251471153936 2208 23 2.7458765136763184 2208 24 3.7469088816135354 2208 25 5.160433406026268 2209 1 2.6523436638915747 2209 2 4.415937557734381 2209 3 4.494234388786765 2209 4 -0.3368087528026642 2209 5 4.391251312916346 2209 6 4.839627105248937 2209 7 5.014931273632065 2209 8 3.9190781936607806 2209 9 3.3681572217120603 2209 10 2.568388650952688 2209 11 -1.019542589298677 2209 12 2.082166832732348 2209 13 1.4870075207253777 2209 14 2.946608941325014 2209 15 3.227219564115824 2209 16 2.94468758532161 2209 17 0.9958168348559049 2209 18 2.630472565821581 2209 19 3.354213077409465 2209 20 3.2209970722602 2209 21 5.253701125009165 2209 22 3.8514404761771104 2209 23 -0.3228823916346961 2209 24 -3.392529675668019 2209 25 -0.7961803968472019 2210 1 -2.003317378148342 2210 2 -13.832554601493605 2210 3 -7.409423296129871 2210 4 -1.9753574473493494 2210 5 -0.17650357992049948 2210 6 -0.8541794049068265 2210 7 -1.0183457463325993 2210 8 -20.914456185108822 2210 9 -12.8211981579696 2210 10 -6.7864165414392765 2210 11 -10.103888763215624 2210 12 -4.358049893731785 2210 13 -5.1372873362794405 2210 14 -3.479617190658864 2210 15 -5.526705467357207 2210 16 -4.665765332108332 2210 17 -4.047410772837608 2210 18 -4.10661884610581 2210 19 -10.091194182834174 2210 20 -7.056173095797266 2210 21 -5.355722047503303 2210 22 -9.959749212127619 2210 23 -7.434794759567975 2210 24 -10.038757325170723 2210 25 -7.361446868507539 2211 1 -0.1442220070088709 2211 2 4.418670888702472 2211 3 0.3049368060624822 2211 4 1.005750496470025 2211 5 0.15711764135363282 2211 6 -0.8439066822105588 2211 7 -20.66661275531848 2211 8 -13.750240498834312 2211 9 -9.763450668730004 2211 10 -8.019432726090287 2211 11 -10.233125278103993 2211 12 -5.777442843466236 2211 13 -7.132213901257165 2211 14 -5.655729098836769 2211 15 -6.191138249455925 2211 16 -11.637593216021848 2211 17 -8.04304997845567 2211 18 -6.45250598144775 2211 19 -9.217616317798559 2211 20 -7.24931916038485 2211 21 -5.052711337442641 2211 22 -6.596890846701861 2211 23 -8.554483777665585 2211 24 -21.96430289641591 2211 25 -21.70932876837917 2212 1 -1.874562564284414 2212 2 -13.832554601493605 2212 3 -8.234936774948869 2212 4 -19.60822009176527 2212 5 -20.143127456685583 2212 6 -20.870867466524285 2212 7 -10.296875913213215 2212 8 -9.65129829037198 2212 9 -5.822738594426596 2212 10 -6.222167504212063 2212 11 -6.433060778619989 2212 12 -3.821624990009063 2212 13 -6.845952291350045 2212 14 -4.790945817103022 2212 15 -4.732417919218566 2212 16 -4.828666487435676 2212 17 -5.000170721489724 2212 18 -4.031169552139186 2212 19 -4.523447071152532 2212 20 -4.639902235720246 2212 21 -2.4212226893518256 2212 22 -3.8223294431930874 2212 23 -4.164287699234215 2212 24 -3.16825026483696 2212 25 -3.4935392946949584 2213 1 -18.73245730112652 2213 2 -13.832554601493605 2213 3 -8.369967286009128 2213 4 -5.070538932344977 2213 5 -20.143127456685583 2213 6 -10.420142828843126 2213 7 -20.66661275531848 2213 8 -10.378224301050851 2213 9 -7.682845383137504 2213 10 -6.528384132739905 2213 11 -5.959052388078415 2213 12 -4.593459467507159 2213 13 -19.10647860713952 2213 14 -11.89336763251111 2213 15 -6.262280619447402 2213 16 -5.506774801699596 2213 17 -4.186059356119777 2213 18 -4.290570772581596 2213 19 -5.199095431183043 2213 20 -5.309677216650833 2213 21 -3.5022104849277227 2213 22 -4.327325629310554 2213 23 -3.93204132242262 2213 24 -2.9932884036622838 2213 25 -3.8339664495385897 2214 1 -18.73245730112652 2214 2 -13.832554601493605 2214 3 -12.437166738559414 2214 4 -19.60822009176527 2214 5 -20.143127456685583 2214 6 -20.870867466524285 2214 7 -16.688535183649 2214 8 -15.086462930471384 2214 9 -14.159864714955576 2214 10 -13.895652016504599 2214 11 -14.978279115355589 2214 12 -12.522865337927765 2214 13 -19.10647860713952 2214 14 -16.74165807413684 2214 15 -23.666628445534357 2214 16 -18.21646270648245 2214 17 -23.4106970372792 2214 18 -17.85424357361784 2214 19 -15.115448047455008 2214 20 -13.821501152912045 2214 21 -11.371890569000358 2214 22 -12.42432198779236 2214 23 -12.266437783551245 2214 24 -11.222313014459754 2214 25 -10.965652545782206 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 87012.0 3 5.0 4 421.0 5 1772.0 6 2447.0 7 2257.0 8 1837.0 9 1748.0 10 1532.0 11 1430.0 12 1437.0 13 1495.0 14 2172.0 15 4635.0 16 7150.0 17 7446.0 18 7672.0 19 8730.0 20 10415.0 21 12166.0 22 12810.0 23 13982.0 24 14662.0 25 14969.0 26 12896.0 27 10008.0 28 10440.0 29 12205.0 30 11872.0 31 13855.0 32 17692.0 33 22196.0 34 27270.0 35 31377.0 36 31328.0 37 32087.0 38 25465.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.881633613671404 18.561390195051896 12.883463576063518 24.673512615213184 2 12.245250882608213 20.317934729409615 43.37635980396635 24.06045458401582 3 20.495314888244025 25.090933256540325 28.488137695766785 25.925614159448862 4 11.224504609131161 17.198228932278433 37.81471534683409 33.76255111175631 5 11.573247320718256 39.376007960332934 35.880864196047234 13.16988052290158 6 31.443975472237636 36.928261916225324 18.46083158792577 13.16693102361127 7 27.304105871937267 31.35317857266048 21.713175353270557 19.629540202131697 8 23.69205850771049 40.61160589408336 20.153081941474245 15.543253656731906 9 26.20732043852387 16.636352993318447 19.920264586103375 37.2360619820543 10 14.661758562852919 31.36231687379798 33.89941921242745 20.076505350921646 11 32.49664304611989 23.39187506370457 24.270001019273124 19.841480870902412 12 24.133939974457217 25.909296509152828 31.741343834255716 18.215419682134243 13 28.793090193997315 22.107709056101257 24.74426724661689 24.35493350328454 14 19.589428244432032 24.926691198484775 27.521854622435782 27.962025934647407 15 23.69746490136267 32.115118801175434 23.05450042885795 21.132915868603945 16 19.463566025129854 29.012478717213735 31.30110562727645 20.222849630379965 17 20.916588944714977 29.643438906247987 29.788066206208875 19.65190594282816 18 20.508479251135622 27.974208988663925 32.69237705457145 18.824934705629 19 22.655193034391207 27.748561201423318 27.832747352483384 21.763498411702088 20 22.445915097698382 29.10825357599112 28.8768351169658 19.5689962093447 21 20.64486953178251 27.457967037768494 27.555184647919322 24.34197878252967 22 21.2618023199018 30.24451985847835 27.28815287057055 21.205524951049306 23 20.126648701422237 29.913595397329463 28.67073219172498 21.28902370952332 24 21.85165813283415 29.340325570298592 28.969379140744007 19.838637156123255 25 21.718842118907347 28.015855427933484 28.38610810290976 21.879194350249414 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 814.0 1 814.0 2 1506.5 3 2199.0 4 2199.0 5 2199.0 6 2868.5 7 3538.0 8 3538.0 9 3538.0 10 4066.5 11 4595.0 12 4595.0 13 4595.0 14 5520.5 15 6446.0 16 6446.0 17 6446.0 18 8817.5 19 11189.0 20 11189.0 21 11189.0 22 15567.5 23 19946.0 24 19946.0 25 19946.0 26 26245.5 27 32545.0 28 32545.0 29 32545.0 30 39781.0 31 47017.0 32 47017.0 33 47017.0 34 50501.5 35 53986.0 36 53986.0 37 53986.0 38 55358.0 39 56730.0 40 56730.0 41 56730.0 42 57846.0 43 58962.0 44 58962.0 45 58962.0 46 61478.5 47 63995.0 48 63995.0 49 63995.0 50 56619.0 51 49243.0 52 49243.0 53 49243.0 54 39399.0 55 29555.0 56 29555.0 57 29555.0 58 23813.5 59 18072.0 60 18072.0 61 18072.0 62 14375.5 63 10679.0 64 10679.0 65 10679.0 66 8060.0 67 5441.0 68 5441.0 69 5441.0 70 3928.5 71 2416.0 72 2416.0 73 2416.0 74 1698.0 75 980.0 76 980.0 77 980.0 78 684.0 79 388.0 80 388.0 81 388.0 82 251.5 83 115.0 84 115.0 85 115.0 86 73.0 87 31.0 88 31.0 89 31.0 90 19.5 91 8.0 92 8.0 93 8.0 94 4.5 95 1.0 96 1.0 97 1.0 98 1.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.04905271114842 2 36.00282317762005 3 5.734683948188843 4 13.694708421296614 5 13.119840966562467 6 14.559410974894183 7 9.19370297749184 8 7.434019707951463 9 0.11714516603917786 10 1.5226783435965863 11 5.7614122570177475 12 5.822803841359135 13 15.794759998580057 14 1.6579904070429092 15 2.8615995639944622 16 3.111968644352705 17 2.8313214016492205 18 1.5798936296834574 19 1.5283163462401832 20 0.2929673225543075 21 4.417897108539903 22 1.6728162658464416 23 0.11735398095190366 24 8.97507376386792 25 0.11923331516643593 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 478893.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.95431105572864 #Duplication Level Percentage of deduplicated Percentage of total 1 96.9413486675128 84.29468186196752 2 1.9382329293411542 3.3707541807277366 3 0.43948007643162856 1.146440618064937 4 0.19083881807601644 0.6637703179395816 5 0.10788784011753702 0.46906564043555166 6 0.06542645414549257 0.34134673470243165 7 0.04637386594520294 0.28226852949790865 8 0.0327219969150068 0.22762549584896757 9 0.026853114604021743 0.21014946720839126 >10 0.17662821093499834 2.994303291169988 >50 0.014588717663574623 0.9116076173762733 >100 0.01735554196882896 3.135291356357544 >500 0.001760707156257983 1.1062646446080455 >1k 5.030591875022808E-4 0.8464302440951239 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 2009 0.4195091596661467 No Hit CCCATGTACTCTGCGTTGATACCAC 1861 0.38860455258272725 No Hit GAGTACATGGGAAGCAGTGGTATCA 971 0.20275928025675882 No Hit GTCCTACAGTGGACATTTCTAAATT 819 0.1710194135224361 No Hit CATGTACTCTGCGTTGATACCACTG 727 0.15180844155166184 No Hit GCGTTGATACCACTGCTTCCCATGT 702 0.14658806873351668 No Hit ACGCAGAGTACATGGGAAGCAGTGG 647 0.1351032485335973 No Hit CTGTAGGACGTGGAATATGGCAAGA 598 0.12487131781003273 No Hit GTATCAACGCAGAGTACATGGGAAG 594 0.12403605815912949 No Hit GTCCTACAGTGTGCATTTCTCATTT 494 0.10315456688654878 No Hit GCTTCCCATGTACTCTGCGTTGATA 492 0.10273693706109716 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 2.0881491272580722E-4 0.0 0.0 0.0 7 0.0 2.0881491272580722E-4 0.0 0.0 0.0 8 0.0 2.0881491272580722E-4 0.0 0.0 0.0 9 0.0 2.0881491272580722E-4 0.0 0.0 0.0 10 0.0 2.0881491272580722E-4 0.0 0.0 0.0 11 0.0 2.0881491272580722E-4 0.0 0.0 0.0 12 0.0 2.0881491272580722E-4 0.0 0.0 2.0881491272580722E-4 13 0.0 2.0881491272580722E-4 0.0 0.0 2.0881491272580722E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTTATA 20 0.00820968 27.257528 2 CTGGACT 30 3.4336533E-4 21.420635 4 CCCCTAC 35 0.005980599 19.02143 1 GTTCTAG 35 0.005980599 19.02143 1 GAACTAA 35 0.005980599 19.02143 1 CCCCTGT 55 2.3767732E-4 16.946365 1 GTAGCAC 40 0.0020973934 16.363697 3 TCTACAC 80 2.3404937E-8 16.363697 3 TGTAGAA 235 0.0 15.65858 2 CTGTACT 55 7.037521E-5 15.578644 4 GTCTTAC 60 4.6120226E-4 15.534167 1 GTCCTAC 490 0.0 15.217144 1 TCCTATA 55 0.0039710426 14.867742 2 CTGTAGG 445 0.0 14.362248 1 GTCCTAA 195 0.0 14.3392315 1 CTACACC 45 0.005193881 14.280424 4 TAGGACC 210 0.0 14.280424 4 TGTAGGA 450 0.0 14.234487 2 GCAATAT 45 9.749308E-4 14.076494 12 CCTGTAC 70 2.9680421E-5 14.026027 3 >>END_MODULE