FastQCFastQC Report
Thu 26 May 2016
SRR1926529_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926529_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478893
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC51461.074561540887004No Hit
GTACATGGGAAGCAGTGGTATCAAC49501.0336338179927458No Hit
GAGTACATGGGAAGCAGTGGTATCA23080.48194481857116306No Hit
CATGTACTCTGCGTTGATACCACTG18730.39111033153543695No Hit
CTGTAGGACGTGGAATATGGCAAGA17090.35686468584840453No Hit
GTCCTACAGTGGACATTTCTAAATT16580.34621512529938836No Hit
GCGTTGATACCACTGCTTCCCATGT16230.33890660335398515No Hit
ACGCAGAGTACATGGGAAGCAGTGG15370.3209485208595657No Hit
GTATCAACGCAGAGTACATGGGAAG14960.3123871094378076No Hit
GTATCAACGCAGAGTACTTTTTTTT14490.30257280853969465No Hit
ACTCTGCGTTGATACCACTGCTTCC11890.2482809312309848No Hit
CTTTAGGACGTGAAATATGGCGAGG11570.24159885402375894No Hit
GTCCTACAGTGTGCATTTCTCATTT10990.22948758908566216No Hit
GCTTCCCATGTACTCTGCGTTGATA10860.22677299522022662No Hit
GTCCTAAAGTGTGTATTTCTCATTT10170.21236476624214595No Hit
GATATACACTGTTCTACAAATCCCG10110.2111118767657911No Hit
GGTATCAACGCAGAGTACATGGGAA9460.19753890743861363No Hit
TATCAACGCAGAGTACATGGGAAGC9340.19503312848590396No Hit
CTGTAGGACCTGGAATATGGCGAGA9200.19210971970774265No Hit
GGTATCAACGCAGAGTACTTTTTTT8860.1850100126750652No Hit
CAGTGGTATCAACGCAGAGTACATG7210.150555552075307No Hit
TATCAACGCAGAGTACTTTTTTTTT6760.14115888100264568No Hit
GTGTATATCAATGAGTTACAATGAG6590.13760902748630696No Hit
CTGAAGGACCTGGAATATGGCGAGA6530.1363561380099521No Hit
GTGTATATCAATGAGTTACAATGAA6420.13405917396996825No Hit
ACATGGGAAGCAGTGGTATCAACGC6260.13071813536635532No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6230.1300916906281779No Hit
CATGGGAAGCAGTGGTATCAACGCA6090.1271682818500166No Hit
GATATACACTGTTCTACAATGCCGG5820.1215302792064198No Hit
GTTCTACAGTGTGGTTTTTATCATT5790.12090383446824238No Hit
GTGGTATCAACGCAGAGTACATGGG5720.11944213007916173No Hit
CTGTAGAACATATTAGATGAGTGAG5530.1154746467373714No Hit
ATACCACTGCTTCCCATGTACTCTG5400.1127600528719359No Hit
GCAGTGGTATCAACGCAGAGTACAT5370.11213360813375847No Hit
GGGAAGCAGTGGTATCAACGCAGAG5000.1044074563629036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3550.014.7639354
CTACACT852.586421E-713.453344
CTAGAAC500.001461218213.34262853
CTAGGAC500.001461218213.34262853
CCCTAGA655.260605E-513.2001652
CAATTCC655.4342738E-513.1545729
AGAGTGT957.053313E-813.04029756
GACGTGG3750.012.9180087
GGCGAGG2650.012.87537519
GTAGGAC8150.012.8631843
GTATAGA603.9669854E-412.7099351
AATTCCC604.1352992E-412.64750810
GATATAC4450.012.638531
TGTAGGA8500.012.5617262
ACTGTTC4200.012.4411618
TCCACAA550.002983478612.1334852
ACCCTAG550.002985827612.1322111
TCTACAC550.002990530312.12966253
TAGAGTG1103.628884E-812.128395
CTAGAGT959.967735E-712.0371984
Produced by FastQC (version 0.11.5)