##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926529_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 478893 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.358558174790613 31.0 28.0 31.0 22.0 34.0 2 28.82461217850334 31.0 28.0 33.0 23.0 34.0 3 29.165429438308767 31.0 29.0 33.0 25.0 34.0 4 32.876016145569054 35.0 33.0 37.0 27.0 37.0 5 32.57763842862602 35.0 33.0 37.0 25.0 37.0 6 32.65904074605392 35.0 33.0 37.0 26.0 37.0 7 32.50892996974272 35.0 33.0 37.0 25.0 37.0 8 32.480798424700296 35.0 33.0 37.0 25.0 37.0 9 33.71488620631331 37.0 34.0 39.0 22.0 39.0 10 33.61518334993412 37.0 33.0 39.0 22.0 39.0 11 33.83238009325674 37.0 34.0 39.0 25.0 39.0 12 33.64260074797502 37.0 34.0 39.0 23.0 39.0 13 33.778081951500646 37.0 34.0 39.0 25.0 39.0 14 34.42399868028975 38.0 34.0 40.0 19.0 41.0 15 34.5385983925428 38.0 34.0 40.0 23.0 41.0 16 34.50193675831553 38.0 34.0 40.0 22.0 41.0 17 34.45784548949348 38.0 34.0 40.0 22.0 41.0 18 34.44875160004427 38.0 34.0 40.0 21.0 41.0 19 34.4710843549603 39.0 34.0 40.0 19.0 41.0 20 34.47785204628174 39.0 34.0 40.0 19.0 41.0 21 34.38469971371475 39.0 34.0 40.0 18.0 41.0 22 34.3276848899441 39.0 34.0 40.0 18.0 41.0 23 33.99006876275076 39.0 34.0 40.0 16.0 41.0 24 34.06835138538254 39.0 34.0 41.0 16.0 41.0 25 33.87396140682783 39.0 34.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.625585792032993 1101 2 0.8351650765105525 1101 3 0.7492970765929208 1101 4 0.6647183271199566 1101 5 0.5537240861818233 1101 6 0.2124922394190918 1101 7 0.4788533604815939 1101 8 0.6219320921896596 1101 9 0.7405296396446204 1101 10 0.8649244479069225 1101 11 0.6756439206287936 1101 12 0.7302116404815138 1101 13 0.6140707868649571 1101 14 0.7482898185877147 1101 15 0.8165661771986379 1101 16 0.5688628698064306 1101 17 0.7856946572513479 1101 18 0.8553090247418567 1101 19 0.44687029014464485 1101 20 0.5270141460772066 1101 21 0.6983271847564154 1101 22 0.7590956220298537 1101 23 0.9810416606459356 1101 24 0.925092259557573 1101 25 1.195741797509129 1102 1 -0.0657704189530044 1102 2 0.10741692080885556 1102 3 -0.11060106571403239 1102 4 -0.21179362301126048 1102 5 0.029070549248324085 1102 6 -0.45974548998395903 1102 7 -0.34523615676018693 1102 8 -0.21346230390886944 1102 9 0.08746512305097554 1102 10 0.2214350153545226 1102 11 0.004839285181972741 1102 12 -0.087320211651992 1102 13 0.03294758687417243 1102 14 0.45791905773725006 1102 15 -0.0011140253645152143 1102 16 0.03836317370876685 1102 17 -0.024281405038550474 1102 18 0.23307259160469584 1102 19 -0.057564379494792206 1102 20 0.10490244252456904 1102 21 0.1448706197458023 1102 22 0.10127524991512615 1102 23 0.35835873592441203 1102 24 0.24519935992599784 1102 25 0.3789344139786195 1103 1 0.2624204479071217 1103 2 0.24634263045430416 1103 3 -0.04009777572967366 1103 4 -0.06320395750294949 1103 5 0.3237838847311991 1103 6 -0.16345134985809295 1103 7 0.18283917253679505 1103 8 0.18544531380808849 1103 9 0.43901875994656336 1103 10 0.4287503045268295 1103 11 0.4139542137098928 1103 12 0.44716043655254367 1103 13 0.5080562578637995 1103 14 0.8831719267707925 1103 15 0.2637535990398874 1103 16 0.5143137463212852 1103 17 0.39307341171350174 1103 18 0.3500595988534059 1103 19 0.5594927862224992 1103 20 0.4444216629594919 1103 21 0.5263580389889242 1103 22 0.43038265506684326 1103 23 0.5021985238368529 1103 24 0.5555804249016987 1103 25 0.8007047360507684 1104 1 0.003951092902021003 1104 2 0.15057917061317383 1104 3 -0.025249539235364438 1104 4 -0.18497296519429085 1104 5 -0.2743867002002034 1104 6 -0.6353410003664557 1104 7 -0.3578412414887211 1104 8 -0.335197216653512 1104 9 0.1386754580586924 1104 10 0.07000699576145308 1104 11 -0.06538033082765082 1104 12 -0.010899707187206786 1104 13 -0.3534810353813498 1104 14 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1.0074884467149658 1107 13 0.7767932145827885 1107 14 1.4397117144488618 1107 15 1.3790675451427816 1107 16 1.042067579911702 1107 17 0.8530248680241783 1107 18 0.9225462153128916 1107 19 0.9928359492748555 1107 20 0.9057568412554744 1107 21 1.1866996595315271 1107 22 1.079178136420623 1107 23 1.3996216251888214 1107 24 1.3459238601616548 1107 25 1.508943552855051 1108 1 1.073506458804225 1108 2 0.8534974993975517 1108 3 0.7744524384006866 1108 4 0.7667081481496396 1108 5 -0.46130098264251274 1108 6 0.3844449034211266 1108 7 0.9802000999118476 1108 8 0.9804379145390456 1108 9 1.3677559513634137 1108 10 1.314535299706094 1108 11 1.1228182016916861 1108 12 0.8377101125542481 1108 13 0.778900335085261 1108 14 1.2611568591683806 1108 15 1.074080063758828 1108 16 0.8995550073361045 1108 17 1.1572973853451956 1108 18 1.3753208154726124 1108 19 1.1097797881732134 1108 20 1.1011740565109207 1108 21 1.0554646333071886 1108 22 1.2139960768924496 1108 23 1.3977006651404693 1108 24 1.0779688192603132 1108 25 1.2484025543875035 1109 1 0.6070463092142759 1109 2 -0.5358795121554714 1109 3 0.7516375566721933 1109 4 0.8656971518964909 1109 5 -0.12837102516741794 1109 6 0.33484245589549744 1109 7 0.8011353069544498 1109 8 0.8910887416015854 1109 9 1.2572068204679496 1109 10 -0.8437556365519967 1109 11 0.3369426062151817 1109 12 0.6494571561216844 1109 13 0.76111486854699 1109 14 1.28448732341991 1109 15 0.948455470330444 1109 16 1.0137629277063027 1109 17 0.9474283120586193 1109 18 1.0079803406821881 1109 19 0.8477246733957386 1109 20 0.9450008435085806 1109 21 1.170700878048386 1109 22 0.9020132572952022 1109 23 1.1074362266993205 1109 24 0.9942768507009063 1109 25 1.264543270067179 1110 1 0.5524689915964416 1110 2 -0.1856962044971553 1110 3 0.33490222427032634 1110 4 0.7681067221086977 1110 5 0.8031528167700301 1110 6 0.4494127278118114 1110 7 0.6779848036047582 1110 8 0.772823954584787 1110 9 1.0094071094611294 1110 10 0.7005949647210059 1110 11 0.5790027573992163 1110 12 0.6194905336399259 1110 13 0.7167941219414686 1110 14 0.8710360044406968 1110 15 0.9943361233117329 1110 16 0.44028462010769687 1110 17 0.9464714699659282 1110 18 0.8876809580767073 1110 19 0.6116772522419183 1110 20 0.6349556435420212 1110 21 0.8091250292801888 1110 22 1.0250428492416006 1110 23 1.197586873351419 1110 24 1.0300949880084929 1110 25 0.8965785224585403 1111 1 0.33764308326712467 1111 2 0.2899516184962607 1111 3 0.6001366799612988 1111 4 0.38668587242199237 1111 5 0.8606679077751025 1111 6 0.38001705657767104 1111 7 0.7131588952754413 1111 8 0.8800360778834602 1111 9 1.1229740951619718 1111 10 0.7054579406901524 1111 11 0.7723713222044637 1111 12 0.9046847083972054 1111 13 0.5630523560069705 1111 14 0.9002655024782911 1111 15 0.7515488204128786 1111 16 0.7671454615316406 1111 17 0.5321028822085765 1111 18 0.6765618137309559 1111 19 0.5031511212772415 1111 20 0.49886404171503074 1111 21 0.841131731293089 1111 22 0.7508656819896586 1111 23 0.8767653029688063 1111 24 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19 0.6011700225760706 2202 20 0.448365117056305 2202 21 0.5174708540616848 2202 22 0.7080934914942176 2202 23 0.83872966789977 2202 24 0.47688430147849914 2202 25 0.7449054371092743 2203 1 -0.32620422620723843 2203 2 -0.22266726703180595 2203 3 -0.23321819711013347 2203 4 -0.6977785071614377 2203 5 -0.4856275746466423 2203 6 -0.4216958142259628 2203 7 -0.34497760010414424 2203 8 -0.045893678040350494 2203 9 -0.03605172572626003 2203 10 0.23792435400245893 2203 11 -0.10461536951038397 2203 12 0.0024975881055908644 2203 13 -0.15992820579626965 2203 14 0.33664253512998243 2203 15 0.05317838876254655 2203 16 0.13024793216752784 2203 17 0.16074054238627156 2203 18 0.04230411040833815 2203 19 0.149941535175536 2203 20 -0.02967853876519655 2203 21 0.13122390976715792 2203 22 0.13594276548457174 2203 23 0.5694754764964358 2203 24 0.3156224213862586 2203 25 0.25945882068076287 2204 1 -0.21673894302673347 2204 2 -0.40959087516838366 2204 3 -0.45450896522467943 2204 4 -0.6212992288586818 2204 5 -0.2787864861565623 2204 6 -0.45199818229505695 2204 7 -0.3903838024812458 2204 8 -0.30266031819117245 2204 9 -0.06957824594386608 2204 10 0.08762029591235887 2204 11 -0.23253539072306495 2204 12 -0.2643800398042302 2204 13 -0.07286444246913959 2204 14 -0.04735937634205101 2204 15 -0.2937596136909022 2204 16 -0.3371710852309491 2204 17 -0.4988935972036401 2204 18 -0.248468374884105 2204 19 -0.060882758002314574 2204 20 -0.3591219099491667 2204 21 -0.3154238752854752 2204 22 -0.23239877027831568 2204 23 -0.0058222440148796295 2204 24 -0.2251697322541304 2204 25 -0.2694944444106824 2205 1 0.0071310176085326304 2205 2 0.3417241105788662 2205 3 0.2942354354811698 2205 4 -0.11259487158977777 2205 5 -0.0777548586283956 2205 6 0.019148485276545557 2205 7 0.07958079188099987 2205 8 -0.006290810024118798 2205 9 0.14490582717499478 2205 10 -0.053332036561279494 2205 11 0.023372147397381582 2205 12 0.21128848198210903 2205 13 0.14606230292699252 2205 14 0.49643671885726093 2205 15 0.6284724120092946 2205 16 0.45189388920459805 2205 17 0.3659965661373832 2205 18 0.33683602310693317 2205 19 0.3784271148541194 2205 20 0.3670952295467842 2205 21 0.2087746903902854 2205 22 0.44898725297855435 2205 23 0.4257193151905838 2205 24 0.7473852426338823 2205 25 0.8651968591502381 2206 1 0.14733575466192406 2206 2 0.35378140573030237 2206 3 0.44410831116697125 2206 4 0.23774970220922853 2206 5 0.41267736403987954 2206 6 0.3232794679251896 2206 7 0.533489864542581 2206 8 0.3729438793019 2206 9 0.773092298471667 2206 10 0.6262617231960661 2206 11 0.6151609203132153 2206 12 0.7332899093428296 2206 13 0.5760547200731665 2206 14 0.9640952897123398 2206 15 0.6924471655665485 2206 16 0.7293204024299911 2206 17 0.9292660027759538 2206 18 0.6422828539200864 2206 19 0.5374300810881181 2206 20 0.5083203064904467 2206 21 0.7737558447682673 2206 22 0.7473195826988785 2206 23 0.8649449719995417 2206 24 0.5563837483656258 2206 25 0.7307266553052898 2207 1 -0.6553231781717912 2207 2 0.4369044255753387 2207 3 0.4142937007245848 2207 4 0.547500833624504 2207 5 0.6660373893630478 2207 6 0.5213206294830215 2207 7 0.8197101247894807 2207 8 0.5243596098953134 2207 9 0.7609910742332815 2207 10 0.5026330384324567 2207 11 0.5501590809613504 2207 12 0.5832130558037179 2207 13 0.3847222023284118 2207 14 -5.35088302304592 2207 15 -2.0842355562179407 2207 16 -0.6130314584522765 2207 17 -0.24739732739198672 2207 18 0.011596191074708884 2207 19 0.47300713852437326 2207 20 0.31405052918034926 2207 21 0.6168006707712834 2207 22 0.3551355448510023 2207 23 0.7472592473662019 2207 24 0.45838764379918473 2207 25 0.42341516035423865 2208 1 0.5102667683724356 2208 2 0.7533795412504638 2208 3 0.5990282729349623 2208 4 0.6152064703192366 2208 5 0.8035032879132018 2208 6 0.84399650553339 2208 7 0.9259864308903758 2208 8 0.9047289233035158 2208 9 1.0460375287070818 2208 10 1.0255064535773926 2208 11 0.6519873421664997 2208 12 0.8101207219872393 2208 13 0.5061554673108475 2208 14 0.8591942029915316 2208 15 0.8921799698630934 2208 16 0.7590291912409697 2208 17 0.7377927049437716 2208 18 0.9453973705716265 2208 19 0.6156423865479539 2208 20 0.7194879088775394 2208 21 0.9335244179485613 2208 22 0.7781943407368388 2208 23 1.1729040840959044 2208 24 1.10330497382472 2208 25 1.0331825532890022 2209 1 -0.4828261938533345 2209 2 -0.1264248868492679 2209 3 0.3986121566956342 2209 4 0.40274811951111644 2209 5 0.15095898164290134 2209 6 0.2788598333430983 2209 7 0.40354607171348533 2209 8 0.6208614193042408 2209 9 0.6198571779727331 2209 10 0.1865896126182207 2209 11 0.007336403036141803 2209 12 0.43966661430793863 2209 13 0.3400901154767837 2209 14 0.5477900770747084 2209 15 0.6962228273767508 2209 16 0.7609130530073145 2209 17 0.7767281249602433 2209 18 0.7076154914365915 2209 19 0.49713259087660333 2209 20 0.5420360539089373 2209 21 0.3031697901922712 2209 22 0.7049546039583632 2209 23 0.7967164246882703 2209 24 0.7080860756447862 2209 25 0.7261895873747406 2210 1 -0.3225793028313184 2210 2 -0.510758208310854 2210 3 0.07259181884639432 2210 4 0.3559636335086793 2210 5 0.5849476869670696 2210 6 0.5784180495461726 2210 7 0.7454910663015184 2210 8 0.559463846405265 2210 9 0.963748622728076 2210 10 0.7570698453870293 2210 11 0.58824880348768 2210 12 0.45859998439207317 2210 13 0.6251235086333935 2210 14 0.8617136219986818 2210 15 0.8482813106941123 2210 16 0.8418138397943764 2210 17 0.8944240978059526 2210 18 0.7534941116093705 2210 19 0.8627911398498753 2210 20 1.0897349224283204 2210 21 1.285289392354585 2210 22 0.8686608185076565 2210 23 0.9792228393022882 2210 24 0.9344704631633505 2210 25 0.7018055374266936 2211 1 -1.1389335402901004 2211 2 -0.19356075808557804 2211 3 0.4335482360731042 2211 4 0.738219038880068 2211 5 0.8946600507315736 2211 6 0.8888996876442832 2211 7 1.0821705070123357 2211 8 0.8923865854623756 2211 9 1.1107539588805082 2211 10 0.9417428801978716 2211 11 0.6513780667462541 2211 12 0.9304200025148504 2211 13 0.9577992618615099 2211 14 1.1413220314728676 2211 15 0.6946026376844685 2211 16 0.9203317360414474 2211 17 0.980475848416333 2211 18 0.9061109028701608 2211 19 1.1287508292455328 2211 20 1.045554349043634 2211 21 1.2376777610525096 2211 22 1.204166683241226 2211 23 1.201893935982298 2211 24 1.0618506514389168 2211 25 0.9899656345849408 2212 1 0.971579875602572 2212 2 -0.0637748963724718 2212 3 0.5111669151236704 2212 4 0.5837556745869819 2212 5 0.5273096895344693 2212 6 0.5717428248991894 2212 7 0.3878851612951024 2212 8 0.48401455243864433 2212 9 0.8443968897370624 2212 10 0.5614023229223335 2212 11 0.49408962505378895 2212 12 0.7362636557656259 2212 13 0.6000593237810179 2212 14 0.6584401945275644 2212 15 0.7934674467659946 2212 16 -0.211363310398184 2212 17 0.5332880259189139 2212 18 0.5144055365130882 2212 19 0.3122776610308762 2212 20 0.5223087350085507 2212 21 0.6546411253455986 2212 22 0.7662538865435948 2212 23 1.204319116580855 2212 24 1.0528594233687372 2212 25 0.4776720329337536 2213 1 0.3266142005453645 2213 2 0.15743029050777224 2213 3 -0.23387503758418404 2213 4 -1.1300887190056947 2213 5 -1.9842082357134352 2213 6 -0.6036194939413946 2213 7 -0.31526129179414397 2213 8 -0.09792671377035589 2213 9 -0.7518845747938272 2213 10 -0.44757937044150964 2213 11 -0.4206701003466691 2213 12 -0.689518190153521 2213 13 -0.49580261367130873 2213 14 -7.850674984724911 2213 15 -3.876326603577251 2213 16 -2.1458980167292268 2213 17 -1.2129171609613323 2213 18 -4.0903618166524325 2213 19 -2.7247551383589013 2213 20 -4.156619563999843 2213 21 -1.939123176713899 2213 22 -1.2934143056760803 2213 23 -4.229052355577807 2213 24 -2.263330507981376 2213 25 -1.895935899941911 2214 1 0.3936132473963063 2214 2 0.09477662577184276 2214 3 -1.5067127334074364 2214 4 -1.863998829717623 2214 5 -0.22704373196194183 2214 6 -0.46299579230149135 2214 7 -0.3008540771889656 2214 8 -0.37871953826873295 2214 9 -4.8359816493594145 2214 10 -1.2533642520310693 2214 11 -0.5132282057796189 2214 12 -0.8128532342672159 2214 13 -1.1572562881831274 2214 14 -2.9133579771241607 2214 15 -1.8055296243265744 2214 16 -1.0942935491485173 2214 17 -0.821932973459802 2214 18 -0.4160772894388032 2214 19 -3.808158124892614 2214 20 -1.3424445267292668 2214 21 -3.4333969504509803 2214 22 -5.324846228837604 2214 23 -5.313613784812599 2214 24 -4.967414385098465 2214 25 -2.9107365940299452 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16387.0 3 15.0 4 327.0 5 1905.0 6 2319.0 7 1958.0 8 1495.0 9 1241.0 10 1076.0 11 973.0 12 924.0 13 903.0 14 868.0 15 909.0 16 974.0 17 972.0 18 1166.0 19 1421.0 20 1750.0 21 2223.0 22 2997.0 23 3663.0 24 4224.0 25 4860.0 26 5752.0 27 6294.0 28 7219.0 29 8793.0 30 10815.0 31 13690.0 32 17516.0 33 22701.0 34 29775.0 35 39381.0 36 54243.0 37 80816.0 38 126348.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.38105439155651 19.733946392183498 9.843147499869378 29.041851716390614 2 11.948198433420366 21.559895561357703 41.43603133159269 25.05587467362924 3 19.799562613706858 25.22908472636203 28.548959484326993 26.42239317560412 4 11.814851021874635 18.381210207048678 36.98154275496733 32.82239601610936 5 12.249914889084291 39.215768180840335 35.490520837031916 13.043796093043461 6 31.855230939523025 37.303776875859526 17.774335021634858 13.066657162982592 7 27.177778335592485 31.67599611760965 21.226174339407937 19.92005120738993 8 24.274940114436344 40.37176121089133 19.752298664211967 15.60100001046036 9 26.024984152156037 16.356439454959695 19.806562158068385 37.812014234815884 10 15.267164412885709 31.039523834386255 33.27478723959693 20.418524513131104 11 32.97638860756057 23.609095408234648 23.694920846393348 19.71959513781143 12 24.007935264943878 25.9961367789089 31.651683633516054 18.344244322631166 13 29.851971108368673 22.359900854679438 25.06948316220951 22.718644874742374 14 20.143220432837474 25.10160626709545 26.67924599269062 28.075927307376457 15 24.189574093099793 31.855104785292472 22.549093308609184 21.406227812998555 16 19.86994627035222 29.058697499785957 31.269067969152957 19.802288260708863 17 21.02215419183872 30.30377700883954 28.891914523953215 19.782154275368523 18 20.827068611236406 28.174961158723 31.95050034247148 19.04746988756912 19 23.30413356024933 27.774100253714355 27.697463900518915 21.2243022855174 20 22.768580544929083 28.795250664038356 28.81070312891532 19.62546566211724 21 20.83225445403139 27.951187324073068 28.014667190097143 23.201891031798404 22 21.792759551716145 30.163314143242854 26.401095860661982 21.642830444379015 23 20.39349082154051 29.66988450447177 28.143029862620665 21.793594811367047 24 22.60085322486004 29.06581692208424 28.624378509424943 19.70895134363078 25 21.761437314807274 28.017323285159733 28.081847093192007 22.139392306840985 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 387.0 1 387.0 2 388.0 3 389.0 4 389.0 5 389.0 6 455.0 7 521.0 8 521.0 9 521.0 10 793.5 11 1066.0 12 1066.0 13 1066.0 14 1493.0 15 1920.0 16 1920.0 17 1920.0 18 3314.5 19 4709.0 20 4709.0 21 4709.0 22 7925.5 23 11142.0 24 11142.0 25 11142.0 26 17032.5 27 22923.0 28 22923.0 29 22923.0 30 31785.0 31 40647.0 32 40647.0 33 40647.0 34 45036.0 35 49425.0 36 49425.0 37 49425.0 38 51915.0 39 54405.0 40 54405.0 41 54405.0 42 56929.0 43 59453.0 44 59453.0 45 59453.0 46 68932.0 47 78411.0 48 78411.0 49 78411.0 50 71140.5 51 63870.0 52 63870.0 53 63870.0 54 50956.5 55 38043.0 56 38043.0 57 38043.0 58 30854.0 59 23665.0 60 23665.0 61 23665.0 62 19160.0 63 14655.0 64 14655.0 65 14655.0 66 11237.5 67 7820.0 68 7820.0 69 7820.0 70 5565.0 71 3310.0 72 3310.0 73 3310.0 74 2330.5 75 1351.0 76 1351.0 77 1351.0 78 943.0 79 535.0 80 535.0 81 535.0 82 359.5 83 184.0 84 184.0 85 184.0 86 117.5 87 51.0 88 51.0 89 51.0 90 30.0 91 9.0 92 9.0 93 9.0 94 5.0 95 1.0 96 1.0 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08728463351938742 2 0.02986053251979043 3 0.02923408778161301 4 0.034454460599758194 5 0.022134380748935565 6 0.3936161104881466 7 0.17477808195150063 8 0.1875157916277749 9 0.19002157058048458 10 0.014199414065354892 11 0.002923408778161301 12 0.00375866842906453 13 0.0 14 0.06890892119951639 15 0.001879334214532265 16 0.002923408778161301 17 0.00459392807996776 18 0.0043851131672419524 19 0.0016705193018064577 20 0.0010440745636290361 21 2.0881491272580722E-4 22 0.0 23 0.0 24 8.352596509032289E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 478893.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.55557665134054 #Duplication Level Percentage of deduplicated Percentage of total 1 93.48069356883171 69.6950701479151 2 4.627200586187642 6.899672159692813 3 0.8028589922059612 1.795728454008887 4 0.3240170869214769 0.9662912304127299 5 0.17779330724324344 0.662774127313449 6 0.10752793186273239 0.4810084179691242 7 0.07152587895199243 0.37328572055318443 8 0.055568316565462614 0.3314342308065844 9 0.04012503267756322 0.26923904544866484 >10 0.24632786855471614 3.632341371131738 >50 0.030520658073197832 1.629790214152729 >100 0.02632056688192463 4.377282003593605 >500 0.005040108551857903 2.5589814383847 >1k 0.004200090459881585 5.252821908356649 >5k 2.8000603065877236E-4 1.0742795302600476 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 5146 1.074561540887004 No Hit GTACATGGGAAGCAGTGGTATCAAC 4950 1.0336338179927458 No Hit GAGTACATGGGAAGCAGTGGTATCA 2308 0.48194481857116306 No Hit CATGTACTCTGCGTTGATACCACTG 1873 0.39111033153543695 No Hit CTGTAGGACGTGGAATATGGCAAGA 1709 0.35686468584840453 No Hit GTCCTACAGTGGACATTTCTAAATT 1658 0.34621512529938836 No Hit GCGTTGATACCACTGCTTCCCATGT 1623 0.33890660335398515 No Hit ACGCAGAGTACATGGGAAGCAGTGG 1537 0.3209485208595657 No Hit GTATCAACGCAGAGTACATGGGAAG 1496 0.3123871094378076 No Hit GTATCAACGCAGAGTACTTTTTTTT 1449 0.30257280853969465 No Hit ACTCTGCGTTGATACCACTGCTTCC 1189 0.2482809312309848 No Hit CTTTAGGACGTGAAATATGGCGAGG 1157 0.24159885402375894 No Hit GTCCTACAGTGTGCATTTCTCATTT 1099 0.22948758908566216 No Hit GCTTCCCATGTACTCTGCGTTGATA 1086 0.22677299522022662 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1017 0.21236476624214595 No Hit GATATACACTGTTCTACAAATCCCG 1011 0.2111118767657911 No Hit GGTATCAACGCAGAGTACATGGGAA 946 0.19753890743861363 No Hit TATCAACGCAGAGTACATGGGAAGC 934 0.19503312848590396 No Hit CTGTAGGACCTGGAATATGGCGAGA 920 0.19210971970774265 No Hit GGTATCAACGCAGAGTACTTTTTTT 886 0.1850100126750652 No Hit CAGTGGTATCAACGCAGAGTACATG 721 0.150555552075307 No Hit TATCAACGCAGAGTACTTTTTTTTT 676 0.14115888100264568 No Hit GTGTATATCAATGAGTTACAATGAG 659 0.13760902748630696 No Hit CTGAAGGACCTGGAATATGGCGAGA 653 0.1363561380099521 No Hit GTGTATATCAATGAGTTACAATGAA 642 0.13405917396996825 No Hit ACATGGGAAGCAGTGGTATCAACGC 626 0.13071813536635532 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 623 0.1300916906281779 No Hit CATGGGAAGCAGTGGTATCAACGCA 609 0.1271682818500166 No Hit GATATACACTGTTCTACAATGCCGG 582 0.1215302792064198 No Hit GTTCTACAGTGTGGTTTTTATCATT 579 0.12090383446824238 No Hit GTGGTATCAACGCAGAGTACATGGG 572 0.11944213007916173 No Hit CTGTAGAACATATTAGATGAGTGAG 553 0.1154746467373714 No Hit ATACCACTGCTTCCCATGTACTCTG 540 0.1127600528719359 No Hit GCAGTGGTATCAACGCAGAGTACAT 537 0.11213360813375847 No Hit GGGAAGCAGTGGTATCAACGCAGAG 500 0.1044074563629036 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 355 0.0 14.763935 4 CTACACT 85 2.586421E-7 13.45334 4 CTAGAAC 50 0.0014612182 13.3426285 3 CTAGGAC 50 0.0014612182 13.3426285 3 CCCTAGA 65 5.260605E-5 13.200165 2 CAATTCC 65 5.4342738E-5 13.154572 9 AGAGTGT 95 7.053313E-8 13.0402975 6 GACGTGG 375 0.0 12.918008 7 GGCGAGG 265 0.0 12.875375 19 GTAGGAC 815 0.0 12.863184 3 GTATAGA 60 3.9669854E-4 12.709935 1 AATTCCC 60 4.1352992E-4 12.647508 10 GATATAC 445 0.0 12.63853 1 TGTAGGA 850 0.0 12.561726 2 ACTGTTC 420 0.0 12.441161 8 TCCACAA 55 0.0029834786 12.133485 2 ACCCTAG 55 0.0029858276 12.132211 1 TCTACAC 55 0.0029905303 12.1296625 3 TAGAGTG 110 3.628884E-8 12.12839 5 CTAGAGT 95 9.967735E-7 12.037198 4 >>END_MODULE