FastQCFastQC Report
Thu 26 May 2016
SRR1926528_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926528_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614879
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC25330.41195096921508134No Hit
CCCATGTACTCTGCGTTGATACCAC24480.39812711118772964No Hit
GAGTACATGGGAAGCAGTGGTATCA12760.20752050403412703No Hit
CATGTACTCTGCGTTGATACCACTG9140.14864713219999381No Hit
GCGTTGATACCACTGCTTCCCATGT8900.1447439252275651No Hit
ACGCAGAGTACATGGGAAGCAGTGG8040.13075743357636216No Hit
GTCCTACAGTGGACATTTCTAAATT7030.11433143756739131No Hit
GTATCAACGCAGAGTACATGGGAAG6810.11075349784266499No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGTA552.415808E-416.9125211
TTAGACT400.002408138216.0302334
CTCTTTA550.00470167514.4964481
GTATAAA550.00470167514.4964481
TAACATC703.6079276E-513.7402014
GTCCTAC5150.013.675451
CTATGAC652.5155157E-413.4131663
TAGACAG704.779203E-513.3354955
GTCCTAT700.001505396413.288411
GTTTTAA904.9345777E-513.288411
TCTATGG500.00930243713.0778353
GATATAC1953.6379788E-1212.9476811
TGTAGGA4650.012.8697512
TTGCACT756.895041E-512.8241874
CCTGTAC851.2997969E-512.8214083
CTTCTGG1152.6989765E-612.7106521
TAGAAAT1101.0702024E-712.6298824
GGCGAGG2050.012.505980519
TCAGGAC704.704131E-412.4550823
GTGGTAC609.67473E-512.36948815
Produced by FastQC (version 0.11.5)