FastQCFastQC Report
Thu 26 May 2016
SRR1926528_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926528_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614879
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC77631.2625248219568401No Hit
GTACATGGGAAGCAGTGGTATCAAC69271.1265631124172397No Hit
GAGTACATGGGAAGCAGTGGTATCA31850.5179880919660617No Hit
CATGTACTCTGCGTTGATACCACTG26710.4343944093065465No Hit
GCGTTGATACCACTGCTTCCCATGT24010.3904833308667234No Hit
ACGCAGAGTACATGGGAAGCAGTGG21910.35633026985797206No Hit
GTATCAACGCAGAGTACATGGGAAG20220.32884518742711977No Hit
ACTCTGCGTTGATACCACTGCTTCC16780.2728992208223081No Hit
GTCCTACAGTGGACATTTCTAAATT15510.25224475059320617No Hit
CTGTAGGACGTGGAATATGGCAAGA15190.24704047462996787No Hit
GTATCAACGCAGAGTACTTTTTTTT14600.23744509082274726No Hit
TATCAACGCAGAGTACATGGGAAGC13250.21548955160283567No Hit
GCTTCCCATGTACTCTGCGTTGATA12570.2044304651809543No Hit
GGTATCAACGCAGAGTACATGGGAA12100.19678668485994805No Hit
CAGTGGTATCAACGCAGAGTACATG10370.168651067933691No Hit
CTTTAGGACGTGAAATATGGCGAGG10360.1684884343098398No Hit
GTCCTAAAGTGTGTATTTCTCATTT10010.16279625747504795No Hit
GGTATCAACGCAGAGTACTTTTTTT9240.15027346843850578No Hit
GTCCTACAGTGTGCATTTCTCATTT9120.14832186495229144No Hit
CATGGGAAGCAGTGGTATCAACGCA8590.139702282888178No Hit
GCAGTGGTATCAACGCAGAGTACAT8350.13579907591574927No Hit
ATACCACTGCTTCCCATGTACTCTG8040.13075743357636216No Hit
ACATGGGAAGCAGTGGTATCAACGC7880.12815529559474304No Hit
TATCAACGCAGAGTACTTTTTTTTT7690.1250652567415703No Hit
GATATACACTGTTCTACAAATCCCG7520.12230048513609995No Hit
CTGTAGGACCTGGAATATGGCGAGA7370.119860980778332No Hit
GTGGTATCAACGCAGAGTACATGGG7370.119860980778332No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7210.11725884279671285No Hit
GGGAAGCAGTGGTATCAACGCAGAG6640.10798872623719463No Hit
CCATGTACTCTGCGTTGATACCACT6580.10701292449408746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTCGA504.664047E-617.05688917
TATGTCG504.664047E-617.05688916
TAGGCTG350.002119056416.3416755
GTATTAC350.002122534216.3376661
AGGACCG456.5806275E-414.8285565
GATATAC3400.014.2954561
TAGGACC3050.014.0645564
TCCAACG1152.5465852E-1114.00693318
CTAGCAC500.001457711713.3478853
TATACAC4150.013.095173
GGCGAGG2700.012.98464119
CCTAACA603.958226E-412.7143522
ACAGGTC604.0773273E-412.6697658
TGTAGGA8050.012.5564112
CCAACGA1001.4864963E-712.31786219
TAGGACT701.0544975E-412.2562564
TGTCGAG701.11563924E-412.18250218
CTGTAGG8050.012.0756651
GTAGGAC8100.012.0060333
ACTGTTC3200.011.8779058
Produced by FastQC (version 0.11.5)