FastQCFastQC Report
Thu 26 May 2016
SRR1926527_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926527_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences547120
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC25050.45785202514987566No Hit
CCCATGTACTCTGCGTTGATACCAC24040.43939172393624804No Hit
GTCCTACAGTGGACATTTCTAAATT13580.24820880245649948No Hit
GAGTACATGGGAAGCAGTGGTATCA12080.22079251352536922No Hit
CTGTAGGACGTGGAATATGGCAAGA10260.18752741628893113No Hit
CATGTACTCTGCGTTGATACCACTG9020.16486328410586343No Hit
GCGTTGATACCACTGCTTCCCATGT8650.1581005995028513No Hit
ACGCAGAGTACATGGGAAGCAGTGG7650.13982307354876444No Hit
GTCCTAAAGTGTGTATTTCTCATTT7550.13799532095335576No Hit
GTATCAACGCAGAGTACATGGGAAG7310.1336087147243749No Hit
GTCCTACAGTGTGCATTTCTCATTT6320.11551396402982891No Hit
CTTTAGGACGTGAAATATGGCGAGG6160.11258955987717503No Hit
CNCATGTACTCTGCGTTGATACCAC5630.10290247112150899No Hit
GCTTCCCATGTACTCTGCGTTGATA5630.10290247112150899No Hit
ACTCTGCGTTGATACCACTGCTTCC5560.10162304430472291No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGTG250.00349450920.87535
GTGGTAG454.8516948E-520.8485811
GTGCTAG405.266765E-420.103991
AGTCGAC250.005170287619.509748
GGACGGG552.1989035E-718.9703566
AGGACGG451.571007E-518.5558225
GTCGACA351.2743864E-418.3893159
CGGGGAA351.2743864E-418.3893159
TCGACAT351.2743864E-418.38931510
AGGCGTG350.001349271617.480137
GACGGGG350.001349271617.480137
TAGAGGT452.626597E-416.7288044
TAGGACC2700.016.3304984
CTCTCCG350.002694935815.762279
TGTAGGA6400.015.6687062
AACTGTC400.002817916715.6564755
GTCCTAC8150.015.6227321
ACGGGGA455.5199803E-415.1742448
CTGTAGG6200.014.9158651
CCCTGTA751.1858925E-414.6527442
Produced by FastQC (version 0.11.5)