##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926527_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 547120 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.109443997660478 27.0 2.0 30.0 2.0 31.0 2 15.652429083199298 15.0 2.0 30.0 2.0 31.0 3 18.970247843251936 18.0 10.0 30.0 2.0 31.0 4 20.81681897938295 27.0 2.0 35.0 2.0 35.0 5 21.63822379002778 28.0 2.0 35.0 2.0 37.0 6 22.199166544816492 30.0 2.0 35.0 2.0 37.0 7 21.989148632841058 30.0 2.0 35.0 2.0 37.0 8 22.137885655797632 29.0 2.0 35.0 2.0 37.0 9 24.696037432373153 31.0 11.0 37.0 2.0 39.0 10 25.335802017838866 31.0 10.0 37.0 2.0 39.0 11 22.60575193741775 29.0 2.0 37.0 2.0 39.0 12 23.647442974119024 30.0 2.0 37.0 2.0 39.0 13 20.484559146073988 26.0 2.0 35.0 2.0 39.0 14 23.65887373885071 30.0 2.0 37.0 2.0 40.0 15 24.693780157917825 31.0 2.0 38.0 2.0 40.0 16 25.021481576253837 31.0 2.0 38.0 2.0 40.0 17 24.383464322269337 30.0 2.0 38.0 2.0 40.0 18 24.466276136862113 30.0 2.0 37.0 2.0 40.0 19 24.80754496271385 30.0 2.0 38.0 2.0 40.0 20 25.061704927620998 31.0 2.0 38.0 2.0 40.0 21 23.02395269776283 30.0 2.0 37.0 2.0 40.0 22 23.892529975142565 30.0 2.0 37.0 2.0 40.0 23 23.87572379002778 30.0 2.0 38.0 2.0 40.0 24 22.976485962860068 30.0 2.0 38.0 2.0 40.0 25 22.77722071940342 30.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 4.525581352475498 1101 2 9.195045313135328 1101 3 5.783559806623796 1101 4 7.190773696853821 1101 5 6.940610327631077 1101 6 6.422720196800544 1101 7 6.28479405572801 1101 8 4.694748898577814 1101 9 4.7330045506833684 1101 10 4.457018426164524 1101 11 6.935624661354073 1101 12 4.946790659975537 1101 13 9.088937430459282 1101 14 6.508721766477571 1101 15 5.706445758925238 1101 16 5.200259391281659 1101 17 1.3624009523601117 1101 18 3.4895943806950775 1101 19 3.9781240661553277 1101 20 2.1220219064528507 1101 21 0.22220892221911015 1101 22 3.5363431219954258 1101 23 4.7366264545132815 1101 24 5.9925699508466685 1101 25 6.25291949101382 1102 1 5.051276397547628 1102 2 9.741109538028834 1102 3 6.222445329428119 1102 4 7.145918949826637 1102 5 7.311556957974862 1102 6 5.985536730802366 1102 7 6.171137824951309 1102 8 6.071849296803602 1102 9 5.230053702198802 1102 10 4.408685563055219 1102 11 7.243868073977971 1102 12 4.508733039802465 1102 13 8.90157473972948 1102 14 6.538318129256179 1102 15 4.614102239460276 1102 16 5.1521600984677605 1102 17 5.931223249304271 1102 18 5.560047044552732 1102 19 4.445334034288013 1102 20 4.720472942256169 1102 21 4.403262902395696 1102 22 5.128936399796071 1102 23 5.500496136163907 1102 24 6.79088546962711 1102 25 6.5055592978673715 1103 1 4.865301983679885 1103 2 9.001514832949887 1103 3 6.1390233850900096 1103 4 7.603959866844203 1103 5 7.276684392414534 1103 6 6.685951194008716 1103 7 6.493684245303012 1103 8 6.221282895261062 1103 9 5.235835865630577 1103 10 4.357123410114987 1103 11 5.058446592780044 1103 12 4.303150782797466 1103 13 8.214007690735865 1103 14 6.520279291643526 1103 15 5.574990523431374 1103 16 5.348751292187959 1103 17 2.572986679188155 1103 18 1.8010831553093993 1103 19 3.861522201528878 1103 20 4.198780968286538 1103 21 6.28202887009876 1103 22 1.5504648569248864 1103 23 3.8854388816400203 1103 24 5.50516105694085 1103 25 1.5161737147597023 1104 1 4.7916009975302 1104 2 9.425768772251708 1104 3 6.100339694559533 1104 4 6.38282236321696 1104 5 6.625592995085107 1104 6 6.205533704015302 1104 7 6.077495453601223 1104 8 5.885717943417042 1104 9 5.043110619553758 1104 10 3.4700009313372426 1104 11 6.959831624124035 1104 12 4.0112140879879945 1104 13 8.124745253320214 1104 14 6.000641509351844 1104 15 5.408422886275499 1104 16 4.870104818329448 1104 17 5.177700023299586 1104 18 3.1471222051788175 1104 19 3.7546739654056758 1104 20 3.0803877336142342 1104 21 2.507657246889927 1104 22 3.952637518331475 1104 23 4.319455288296645 1104 24 5.199733318544574 1104 25 4.375047449830426 1105 1 3.373605644682982 1105 2 9.128515849958962 1105 3 5.785062903812847 1105 4 5.693073278278323 1105 5 2.0866758752504992 1105 6 4.53124988016155 1105 7 5.537935618724408 1105 8 3.2979132664119817 1105 9 4.079812210412722 1105 10 3.4927265332281827 1105 11 6.557845111114904 1105 12 3.716204541943089 1105 13 4.980878447937002 1105 14 3.5001491695794797 1105 15 4.054940751971447 1105 16 4.358242994266284 1105 17 5.069329578834843 1105 18 2.295473535275928 1105 19 2.3363667231099043 1105 20 1.636083391890331 1105 21 2.417200395681242 1105 22 4.029883623611987 1105 23 1.497811497235844 1105 24 4.475280962999513 1105 25 5.221816163754287 1106 1 6.280876456063385 1106 2 10.937788768820923 1106 3 7.605926971252256 1106 4 9.098763478489072 1106 5 8.783198530677264 1106 6 7.539679806399697 1106 7 7.26607049754422 1106 8 7.261032339120096 1106 9 6.370644665628738 1106 10 5.67923865124715 1106 11 8.075231833981078 1106 12 5.5149400530073684 1106 13 4.302269385766294 1106 14 5.895301961992544 1106 15 5.96221156335185 1106 16 6.159085986890098 1106 17 6.342248440794801 1106 18 6.3123333877522185 1106 19 6.245745016049842 1106 20 5.970119219076842 1106 21 5.998865306113277 1106 22 6.148956331725788 1106 23 6.787590211672089 1106 24 7.452098665844613 1106 25 6.738149145840694 1107 1 3.6411635042816393 1107 2 -13.271905646352945 1107 3 -5.346504159261064 1107 4 1.4963314140032118 1107 5 4.131807625811707 1107 6 4.4343694583136255 1107 7 4.394648848500331 1107 8 4.748162746958435 1107 9 3.214048237359478 1107 10 2.629321664923804 1107 11 5.368483827463049 1107 12 2.6252103446595214 1107 13 4.474802681887233 1107 14 3.4947068566679107 1107 15 3.202337001357499 1107 16 3.050904497346746 1107 17 3.665024954352237 1107 18 -0.7707747849469939 1107 19 1.6147169100692302 1107 20 1.847373251915318 1107 21 -1.2438082287310408 1107 22 1.561252464749849 1107 23 -1.259140280113897 1107 24 1.943366022625419 1107 25 2.7353483270214767 1108 1 5.333435308029099 1108 2 10.41056858045118 1108 3 0.458503204509185 1108 4 5.762641896357039 1108 5 7.488006630358967 1108 6 7.286809177522855 1108 7 7.392610642895267 1108 8 7.368591028136208 1108 9 5.803562658524136 1108 10 5.004570796858918 1108 11 7.202221720242253 1108 12 2.6073867956447607 1108 13 3.5471815559291358 1108 14 4.8730767221850435 1108 15 4.811890552629766 1108 16 5.043244026280451 1108 17 0.6202274311590479 1108 18 3.904115296000729 1108 19 4.833242436014427 1108 20 5.2049665352968475 1108 21 4.88523620114114 1108 22 2.269598787702236 1108 23 5.090672131870239 1108 24 5.786287665659938 1108 25 6.388107322108713 1109 1 3.6781744452699705 1109 2 -13.271905646352945 1109 3 -5.1777715903115755 1109 4 2.598196607093449 1109 5 1.7302120555887477 1109 6 3.2360790301516076 1109 7 4.173307022348272 1109 8 4.47857380873419 1109 9 3.95673483018145 1109 10 2.9260391422309233 1109 11 1.4586186162773203 1109 12 2.0944163369825084 1109 13 3.3278220282327347 1109 14 4.053502511034921 1109 15 4.077225883711904 1109 16 3.80400391625518 1109 17 4.4268848545103445 1109 18 4.541608866678544 1109 19 3.0711474833118757 1109 20 2.172750599522981 1109 21 1.168989583523679 1109 22 3.6072444258264404 1109 23 4.069910452866726 1109 24 5.34836920064663 1109 25 5.622695957303382 1110 1 3.000070333084839 1110 2 8.95614574551001 1110 3 6.473777272369404 1110 4 7.298547766243793 1110 5 8.357637306532833 1110 6 4.007012714024764 1110 7 6.20294420434254 1110 8 7.308958365163516 1110 9 5.586666038289696 1110 10 5.084276750632732 1110 11 3.0432800217500144 1110 12 5.849389930371796 1110 13 9.925728543721362 1110 14 7.769237785343169 1110 15 7.245487320770341 1110 16 6.83801433026067 1110 17 7.196301958190549 1110 18 7.189797099131038 1110 19 6.118586483344391 1110 20 6.234112874068124 1110 21 4.1611628220378485 1110 22 6.079804311002974 1110 23 6.448962601858536 1110 24 7.433160665063898 1110 25 7.676617002234103 1111 1 5.506238051593641 1111 2 9.683469607196752 1111 3 7.634515834943514 1111 4 8.261836810725203 1111 5 8.62211298530763 1111 6 7.872263266730524 1111 7 7.674624820766912 1111 8 7.886371919376458 1111 9 6.3193757950122915 1111 10 5.536824409288602 1111 11 7.4492136275633065 1111 12 6.63915108330081 1111 13 8.365048183561349 1111 14 6.645777000305532 1111 15 6.350890155314779 1111 16 6.0299172451591865 1111 17 7.096313251196268 1111 18 6.9450993824899285 1111 19 5.770920443584323 1111 20 5.751519305000535 1111 21 8.286311042737928 1111 22 7.8191899714917845 1111 23 7.577535643716498 1111 24 7.260175706478826 1111 25 7.90017942432322 1112 1 -1.6174358753970068 1112 2 7.321278592198706 1112 3 5.652126602947973 1112 4 -0.501095573862429 1112 5 3.531530668873291 1112 6 2.536477062858207 1112 7 5.282783342520883 1112 8 5.36310043164729 1112 9 4.1535796759697625 1112 10 3.354286989781855 1112 11 0.562741246388736 1112 12 3.7825127796373827 1112 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4.116345314659213 2204 7 5.108293208954457 2204 8 5.169733466723301 2204 9 3.975422592273631 2204 10 3.3644159936972606 2204 11 4.953983480800247 2204 12 4.975120831568713 2204 13 3.232411847496955 2204 14 4.488411637076101 2204 15 4.503284546354553 2204 16 4.447789729290147 2204 17 -0.6909706534798019 2204 18 2.7178693819951967 2204 19 3.4057594086068335 2204 20 3.6250800138856896 2204 21 5.109824321966322 2204 22 4.797487408139315 2204 23 3.695637640064092 2204 24 5.162093112194906 2204 25 5.011756347482873 2205 1 5.211764545312725 2205 2 9.1522429604582 2205 3 6.373973505489044 2205 4 6.2964263271125205 2205 5 6.784882993492392 2205 6 6.52627913805183 2205 7 6.382286469818549 2205 8 6.188087160826939 2205 9 5.02662820595852 2205 10 4.685698114850684 2205 11 6.8053219511105105 2205 12 6.111945645771897 2205 13 8.799605672618675 2205 14 6.163267481071344 2205 15 5.133068725472423 2205 16 4.5982066298785895 2205 17 5.15038605999311 2205 18 5.263415456650989 2205 19 3.8284984657187664 2205 20 4.067718016790149 2205 21 -1.744630916622949 2205 22 2.4459470264008623 2205 23 3.6166623651854835 2205 24 5.218095353466477 2205 25 5.95654752510255 2206 1 4.055385746653673 2206 2 8.4941399023013 2206 3 5.883309505335209 2206 4 1.6280129663448726 2206 5 5.551870795478177 2206 6 5.859465043993154 2206 7 5.9857612693493465 2206 8 6.060482343156163 2206 9 4.705842225662511 2206 10 3.7385764744325023 2206 11 3.64695929254594 2206 12 4.571848600088636 2206 13 7.766017866786964 2206 14 0.6482106574265529 2206 15 2.675127196458071 2206 16 3.3341705806763002 2206 17 4.4016920743897145 2206 18 -0.5610530035019501 2206 19 1.2293214951593754 2206 20 2.7246608854708043 2206 21 3.7471772482906154 2206 22 -1.3095670785546183 2206 23 -2.6114622942892893 2206 24 1.4484891128008748 2206 25 2.944291821905942 2207 1 0.7298083261961246 2207 2 -13.271905646352945 2207 3 -6.197119794923429 2207 4 -4.703962679121224 2207 5 0.41917212478681876 2207 6 1.1874579233878997 2207 7 2.264854811140978 2207 8 2.4326016906920103 2207 9 0.06945425587348453 2207 10 -0.21340781379721463 2207 11 -1.6681532891249518 2207 12 -2.723430774020482 2207 13 0.035439898094406175 2207 14 -5.838902595025992 2207 15 -1.9424692122801481 2207 16 -0.8224762850319216 2207 17 0.11146936198089819 2207 18 -0.01163445509925154 2207 19 -0.4680850307721407 2207 20 -0.5052688759502217 2207 21 -3.5346897176478684 2207 22 -4.40727545623049 2207 23 -1.57932604821562 2207 24 0.07907224135230706 2207 25 0.7190833313581564 2208 1 4.488501941169659 2208 2 6.282972402427543 2208 3 6.103553912748225 2208 4 7.925934998360226 2208 5 3.6989570955337214 2208 6 6.031067831449597 2208 7 6.420108337975311 2208 8 6.373383166263775 2208 9 5.08902280436271 2208 10 4.7320370612122815 2208 11 6.664967927601236 2208 12 5.982543949278153 2208 13 8.84353768701622 2208 14 3.2280281093277807 2208 15 4.858533751020513 2208 16 5.363749432365438 2208 17 5.885337229164243 2208 18 1.2524222275236525 2208 19 3.6262878638913847 2208 20 4.588008610562962 2208 21 6.808790387713934 2208 22 5.997911586294141 2208 23 1.7049165372008872 2208 24 3.303662571367756 2208 25 4.41609533652494 2209 1 3.5675279032874947 2209 2 5.3800814642807815 2209 3 5.696460394981685 2209 4 0.9218765071845496 2209 5 5.3583123652410904 2209 6 6.009482945890753 2209 7 6.4096237205083 2209 8 5.398213316552386 2209 9 5.067655143049144 2209 10 4.3638430570380855 2209 11 0.3663042387624138 2209 12 3.7564560815251475 2209 13 2.879469196975787 2209 14 4.252882274357077 2209 15 4.815804977868119 2209 16 4.290925822818924 2209 17 2.0447438030903484 2209 18 3.890487338035122 2209 19 4.67826995326115 2209 20 4.625204169145828 2209 21 6.720565688334826 2209 22 5.523493834597826 2209 23 1.093568917280006 2209 24 -1.983374708242419 2209 25 0.8810436393377188 2210 1 -1.9395413211634178 2210 2 -13.271905646352945 2210 3 -7.270227494749106 2210 4 -2.1147429989174853 2210 5 -0.3548725038482452 2210 6 -0.8161436610273185 2210 7 -1.0500982249500481 2210 8 -19.72316154918338 2210 9 -12.178400769194294 2210 10 -6.557574092817809 2210 11 -9.18719610874501 2210 12 -4.329369746781886 2210 13 -5.104954937050293 2210 14 -3.5355695436170755 2210 15 -5.448220470367858 2210 16 -4.622758679322011 2210 17 -4.003936397734922 2210 18 -4.241903270029965 2210 19 -9.039307361065388 2210 20 -6.275200932165951 2210 21 -4.502033472787115 2210 22 -8.915011932081315 2210 23 -7.338207842006703 2210 24 -9.793732859600127 2210 25 -7.677055713763844 2211 1 0.0689635199789933 2211 2 4.657126611711572 2211 3 0.8667062862074459 2211 4 1.9502596770908838 2211 5 1.2148107540703066 2211 6 -0.16495891914048855 2211 7 -19.603265645764527 2211 8 -12.557570151333918 2211 9 -8.556630224748227 2211 10 -6.806725068344498 2211 11 -8.91157941045279 2211 12 -4.486483063572621 2211 13 -6.445335903332586 2211 14 -4.138972939714968 2211 15 -4.678576136207393 2211 16 -10.520895204403509 2211 17 -6.670150575713805 2211 18 -5.130858653672259 2211 19 -7.992430992652018 2211 20 -5.7893910747242145 2211 21 -3.872451416639592 2211 22 -5.405392898363608 2211 23 -7.340100509743774 2211 24 -20.635361818876145 2211 25 -20.430449378922216 2212 1 -1.035062868714249 2212 2 -13.271905646352945 2212 3 -7.364751196067054 2212 4 -18.474471505704816 2212 5 -19.185189245929692 2212 6 -19.759582575054466 2212 7 -9.378374816070204 2212 8 -8.811589496781634 2212 9 -4.793459355611731 2212 10 -5.077669332794887 2212 11 -5.0872731701372516 2212 12 -2.5743311172891055 2212 13 -5.4862853328296985 2212 14 -3.7115066299060757 2212 15 -3.416309150246928 2212 16 -3.3126804323698913 2212 17 -3.9080690618855662 2212 18 -2.894203256198434 2212 19 -3.5709420505474974 2212 20 -3.4649744902287445 2212 21 -1.4209065981205526 2212 22 -2.734115253624914 2212 23 -2.6473574003856477 2212 24 -1.8438771027189382 2212 25 -2.038527981542302 2213 1 -17.767595619805952 2213 2 -13.271905646352945 2213 3 -8.548007918659561 2213 4 -5.796108932605401 2213 5 -19.185189245929692 2213 6 -10.543208306048617 2213 7 -19.603265645764527 2213 8 -10.437781432224316 2213 9 -8.42882415042263 2213 10 -7.3680342552903575 2213 11 -6.848685616828945 2213 12 -5.449320264100823 2213 13 -18.051787516235812 2213 14 -11.970891445657244 2213 15 -7.0039109786899445 2213 16 -6.509670907101114 2213 17 -5.154945897351233 2213 18 -5.2413661050965175 2213 19 -5.875113505385464 2213 20 -6.073733651596502 2213 21 -4.268263527562059 2213 22 -5.025879598986133 2213 23 -4.839477985691708 2213 24 -3.9944261463615263 2213 25 -4.803548794126893 2214 1 -17.767595619805952 2214 2 -13.271905646352945 2214 3 -12.207347978654107 2214 4 -18.474471505704816 2214 5 -19.185189245929692 2214 6 -19.759582575054466 2214 7 -16.16155580120494 2214 8 -14.679120098406178 2214 9 -14.007574567051421 2214 10 -14.06479515581455 2214 11 -14.510192146538365 2214 12 -12.59640840760293 2214 13 -18.051787516235812 2214 14 -16.28161319822519 2214 15 -22.409758931906318 2214 16 -17.663705388814897 2214 17 -22.10348390904714 2214 18 -17.299191151304438 2214 19 -14.951974699822335 2214 20 -13.723498824459575 2214 21 -11.458759846078559 2214 22 -12.369616531992223 2214 23 -12.352931308116942 2214 24 -11.311527621984954 2214 25 -11.012055596538795 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 119690.0 3 14.0 4 490.0 5 2156.0 6 2988.0 7 2863.0 8 2346.0 9 2174.0 10 2095.0 11 1842.0 12 1762.0 13 1931.0 14 2686.0 15 5607.0 16 8099.0 17 8708.0 18 8968.0 19 10681.0 20 12359.0 21 14041.0 22 14791.0 23 15625.0 24 16924.0 25 17047.0 26 14086.0 27 11446.0 28 11979.0 29 13986.0 30 13387.0 31 15225.0 32 19462.0 33 24094.0 34 28996.0 35 32467.0 36 31659.0 37 31981.0 38 22465.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.07283054137609 18.849834850900603 12.587490960148529 25.489843647574784 2 11.662572560928087 20.37738899007889 44.80173655512033 23.158301893872697 3 20.41370903719577 26.315963143909176 27.749988839134073 25.520338979760982 4 11.886020787297078 17.64474436984119 37.48561754691179 32.98361729594995 5 11.444338370549747 38.62413926679184 36.91145746236905 13.020064900289363 6 30.434192643774217 37.890787379358024 18.376779879686485 13.298240097181269 7 26.12420781708024 31.285694949221504 21.956472111726832 20.63362512197142 8 24.491317590285195 40.40247616787311 20.04489537597405 15.061310865867647 9 26.521989734769125 16.27871657166906 20.03476637908162 37.164527314480196 10 15.357705933633817 30.968226587279506 33.52419639048096 20.149871088605718 11 32.2397401624179 23.101549046673306 24.424616232859826 20.234094558048966 12 25.35490453286693 25.785356847884177 31.539620074819023 17.320118544429867 13 29.261164137375822 22.34030684013292 24.205718085979118 24.19281093651214 14 19.26560169127851 25.778250054338635 27.040578423194887 27.915569831187963 15 23.835823903825613 33.19010904761815 22.432087765531612 20.541979283024624 16 18.793562095015094 28.963973899977564 32.69421836616574 19.5482456388416 17 20.67618204756435 30.079085619598168 30.43723049692131 18.807501835916167 18 20.23125463994061 27.34985152190052 34.28303637713437 18.1358574610245 19 22.860561316292273 27.36370550371923 27.777180062698253 21.99855311729025 20 22.992443823623223 28.88101863563538 28.932896314301743 19.193641226439652 21 19.918113274722497 27.634377111530945 27.414634333086408 25.03287528066015 22 21.038833634635715 31.2640393594429 26.995330896902566 20.70179610901882 23 19.152081827580215 30.65533425432056 29.53093693677209 20.661646981327138 24 22.08899943498167 29.14268019761566 29.50964049954859 19.258679867854074 25 21.12305189636464 28.34107664691039 28.782253386594153 21.753618070130816 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 995.0 1 995.0 2 1768.0 3 2541.0 4 2541.0 5 2541.0 6 3305.5 7 4070.0 8 4070.0 9 4070.0 10 4637.5 11 5205.0 12 5205.0 13 5205.0 14 6201.5 15 7198.0 16 7198.0 17 7198.0 18 10047.0 19 12896.0 20 12896.0 21 12896.0 22 18016.5 23 23137.0 24 23137.0 25 23137.0 26 30579.0 27 38021.0 28 38021.0 29 38021.0 30 46531.0 31 55041.0 32 55041.0 33 55041.0 34 59514.5 35 63988.0 36 63988.0 37 63988.0 38 65345.5 39 66703.0 40 66703.0 41 66703.0 42 67530.0 43 68357.0 44 68357.0 45 68357.0 46 71452.5 47 74548.0 48 74548.0 49 74548.0 50 64703.0 51 54858.0 52 54858.0 53 54858.0 54 43180.0 55 31502.0 56 31502.0 57 31502.0 58 24950.5 59 18399.0 60 18399.0 61 18399.0 62 14407.5 63 10416.0 64 10416.0 65 10416.0 66 7866.5 67 5317.0 68 5317.0 69 5317.0 70 3846.0 71 2375.0 72 2375.0 73 2375.0 74 1683.0 75 991.0 76 991.0 77 991.0 78 686.5 79 382.0 80 382.0 81 382.0 82 248.0 83 114.0 84 114.0 85 114.0 86 82.0 87 50.0 88 50.0 89 50.0 90 32.0 91 14.0 92 14.0 93 14.0 94 7.0 95 0.0 96 0.0 97 0.0 98 1.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.56448311156602 2 36.680618511478286 3 5.83546571136131 4 14.060717941219478 5 13.653860213481503 6 15.139823073548763 7 9.527525954086855 8 7.585173270946045 9 0.11240678461763415 10 1.4603743237315396 11 5.854108787834479 12 5.948420821757567 13 16.451418336014036 14 1.6137227664863283 15 2.970280742798655 16 3.0521640590729637 17 2.93354291563094 18 1.5206901593800262 19 1.4676853341131744 20 0.2935370668226349 21 4.512903933323585 22 1.626882585173271 23 0.11057903202222547 24 9.100562947799386 25 0.11460008773212457 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 547120.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.60426086253173 #Duplication Level Percentage of deduplicated Percentage of total 1 96.57144074693227 81.70355364823983 2 2.1247624769251 3.595279177373804 3 0.4819376749524951 1.2232194231348879 4 0.23561801083103207 0.7973715060903787 5 0.13557939121499407 0.5735297090968301 6 0.08082060668024361 0.4102660614386042 7 0.05950056439049036 0.3523800889812639 8 0.0346962416766161 0.2348359905406299 9 0.03482127552194449 0.2651425450042214 >10 0.19815970201410665 3.2568687580147997 >50 0.01891022892517879 1.103530957071696 >100 0.020063281110562367 3.3667825944633964 >500 0.002536736692138613 1.4586269515647206 >1k 0.0011530621327902784 1.6586125889849774 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 2505 0.45785202514987566 No Hit CCCATGTACTCTGCGTTGATACCAC 2404 0.43939172393624804 No Hit GTCCTACAGTGGACATTTCTAAATT 1358 0.24820880245649948 No Hit GAGTACATGGGAAGCAGTGGTATCA 1208 0.22079251352536922 No Hit CTGTAGGACGTGGAATATGGCAAGA 1026 0.18752741628893113 No Hit CATGTACTCTGCGTTGATACCACTG 902 0.16486328410586343 No Hit GCGTTGATACCACTGCTTCCCATGT 865 0.1581005995028513 No Hit ACGCAGAGTACATGGGAAGCAGTGG 765 0.13982307354876444 No Hit GTCCTAAAGTGTGTATTTCTCATTT 755 0.13799532095335576 No Hit GTATCAACGCAGAGTACATGGGAAG 731 0.1336087147243749 No Hit GTCCTACAGTGTGCATTTCTCATTT 632 0.11551396402982891 No Hit CTTTAGGACGTGAAATATGGCGAGG 616 0.11258955987717503 No Hit CNCATGTACTCTGCGTTGATACCAC 563 0.10290247112150899 No Hit GCTTCCCATGTACTCTGCGTTGATA 563 0.10290247112150899 No Hit ACTCTGCGTTGATACCACTGCTTCC 556 0.10162304430472291 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.8277525954086854E-4 0.0 0.0 0.0 0.0 7 1.8277525954086854E-4 0.0 0.0 0.0 0.0 8 1.8277525954086854E-4 0.0 0.0 1.8277525954086854E-4 0.0 9 1.8277525954086854E-4 0.0 0.0 1.8277525954086854E-4 0.0 10 1.8277525954086854E-4 0.0 0.0 1.8277525954086854E-4 0.0 11 1.8277525954086854E-4 0.0 0.0 1.8277525954086854E-4 0.0 12 1.8277525954086854E-4 0.0 0.0 1.8277525954086854E-4 0.0 13 1.8277525954086854E-4 0.0 0.0 1.8277525954086854E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACGGTG 25 0.003494509 20.8753 5 GTGGTAG 45 4.8516948E-5 20.848581 1 GTGCTAG 40 5.266765E-4 20.10399 1 AGTCGAC 25 0.0051702876 19.50974 8 GGACGGG 55 2.1989035E-7 18.970356 6 AGGACGG 45 1.571007E-5 18.555822 5 GTCGACA 35 1.2743864E-4 18.389315 9 CGGGGAA 35 1.2743864E-4 18.389315 9 TCGACAT 35 1.2743864E-4 18.389315 10 AGGCGTG 35 0.0013492716 17.48013 7 GACGGGG 35 0.0013492716 17.48013 7 TAGAGGT 45 2.626597E-4 16.728804 4 TAGGACC 270 0.0 16.330498 4 CTCTCCG 35 0.0026949358 15.76227 9 TGTAGGA 640 0.0 15.668706 2 AACTGTC 40 0.0028179167 15.656475 5 GTCCTAC 815 0.0 15.622732 1 ACGGGGA 45 5.5199803E-4 15.174244 8 CTGTAGG 620 0.0 14.915865 1 CCCTGTA 75 1.1858925E-4 14.652744 2 >>END_MODULE