FastQCFastQC Report
Thu 26 May 2016
SRR1926527_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926527_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences547120
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC72481.3247550811522153No Hit
GTACATGGGAAGCAGTGGTATCAAC67371.2313569235268313No Hit
GAGTACATGGGAAGCAGTGGTATCA31520.5761076180728176No Hit
CTGTAGGACGTGGAATATGGCAAGA29210.533886533118877No Hit
GTCCTACAGTGGACATTTCTAAATT27840.508846322561778No Hit
CATGTACTCTGCGTTGATACCACTG25940.47411902324901295No Hit
GCGTTGATACCACTGCTTCCCATGT21170.38693522444801876No Hit
ACGCAGAGTACATGGGAAGCAGTGG19820.3622605644100015No Hit
GTATCAACGCAGAGTACATGGGAAG19410.35476677876882584No Hit
CTTTAGGACGTGAAATATGGCGAGG18860.3447141394940781No Hit
GTCCTAAAGTGTGTATTTCTCATTT18630.3405103085246381No Hit
GTATCAACGCAGAGTACTTTTTTTT15750.28787103377686796No Hit
ACTCTGCGTTGATACCACTGCTTCC15100.2759906419067115No Hit
GTCCTACAGTGTGCATTTCTCATTT14880.2719695861968124No Hit
GCTTCCCATGTACTCTGCGTTGATA14800.2705073841204855No Hit
CTGTAGGACCTGGAATATGGCGAGA12560.2295657259833309No Hit
CTGAAGGACCTGGAATATGGCGAGA11790.21549203099868403No Hit
TATCAACGCAGAGTACATGGGAAGC11550.21110542476970315No Hit
GGTATCAACGCAGAGTACATGGGAA11500.21019154847199883No Hit
GATATACACTGTTCTACAAATCCCG10290.18807574206755373No Hit
CAGTGGTATCAACGCAGAGTACATG10240.1871618657698494No Hit
GGTATCAACGCAGAGTACTTTTTTT9900.18094750694545986No Hit
GTGTATATCAATGAGTTACAATGAA8500.15535897060973825No Hit
GTGGTATCAACGCAGAGTACATGGG7920.1447580055563679No Hit
TATCAACGCAGAGTACTTTTTTTTT7910.14457523029682703No Hit
CATGGGAAGCAGTGGTATCAACGCA7840.14329580348004092No Hit
ACATGGGAAGCAGTGGTATCAACGC7640.13964029828922356No Hit
GCAGTGGTATCAACGCAGAGTACAT7290.13324316420529317No Hit
GTGTATATCAATGAGTTACAATGAG7190.13141541160988449No Hit
ATACCACTGCTTCCCATGTACTCTG7110.12995320953355755No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7030.12849100745723058No Hit
ACCTGGAATATGGCGAGAAAACTGA6780.12392162596870887No Hit
GTTCTACAGTGTGGTTTTTATCATT6690.12227664863284106No Hit
GGGAAGCAGTGGTATCAACGCAGAG6470.11825559292294197No Hit
GTCCTTCAGTGTGCATTTCTCATTT6460.11807281766340108No Hit
GATATACACTGTTCTACAATGCCGG6430.11752449188477847No Hit
CTGTAGAACATATTAGATGAGTGAG6420.11734171662523762No Hit
CTGCTTCCCATGTACTCTGCGTTGA6210.11350343617487937No Hit
GAATATGGCAAGAAAACTGAAAATC6130.11204123409855242No Hit
ATTCCAGGTCCTTCAGTGTGCATTT6100.1114929083199298No Hit
CCATGTACTCTGCGTTGATACCACT6030.11021348150314374No Hit
CTGTAGGACATGGAATATGGCAAGA5960.10893405468635767No Hit
GACCTGGAATATGGCGAGAAAACTG5940.10856850416727591No Hit
ACAGTGGACATTTCTAAATTTTCCA5930.10838572890773505No Hit
AAGCAGTGGTATCAACGCAGAGTAC5880.10747185261003071No Hit
GCCATATTCCACGTCCTACAGTGGA5840.10674075157186723No Hit
CCACTGCTTCCCATGTACTCTGCGT5570.10180581956426378No Hit
GATACCACTGCTTCCCATGTACTCT5560.10162304430472291No Hit
CTTCCCATGTACTCTGCGTTGATAC5550.10144026904518205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAG607.393464E-817.4703315
GTCCTAG859.749783E-1015.6909451
TAGAACC704.269241E-714.9745694
TGTCGAG456.877584E-414.74202118
GATTTCA400.005358747714.2155216
TAGGACC4050.014.1174394
TCCAACG1257.2759576E-1213.64689918
ACTGTTC5250.013.5795498
TGAAGTG500.001463047813.340985
ATGTCGA500.00152496613.26781917
GATATAC5800.013.14021
CTTAAGG603.9890627E-412.7021921
GTTCAGG603.9890627E-412.7021921
CTCTAGG603.9890627E-412.7021921
CACTGTT5750.012.561737
TAGGACA2750.012.4746834
GTCCTAA5550.012.3588911
AATCCCG3150.012.33403319
TAGGACT1551.8189894E-1212.2958344
TATACAC6800.012.1929823
Produced by FastQC (version 0.11.5)