##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926527_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 547120 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.06285458400351 31.0 28.0 31.0 16.0 34.0 2 28.534869864015207 31.0 28.0 33.0 22.0 34.0 3 28.89226129551104 31.0 28.0 33.0 23.0 34.0 4 32.56488521713701 35.0 32.0 37.0 26.0 37.0 5 32.22230040941658 35.0 32.0 37.0 23.0 37.0 6 32.29676670565872 35.0 33.0 37.0 23.0 37.0 7 32.11442827898816 35.0 33.0 37.0 19.0 37.0 8 32.07722254715602 35.0 33.0 37.0 19.0 37.0 9 33.256137593215385 37.0 33.0 39.0 17.0 39.0 10 33.15515791782424 37.0 33.0 39.0 17.0 39.0 11 33.36609518935517 37.0 33.0 39.0 19.0 39.0 12 33.17365842959497 37.0 33.0 39.0 17.0 39.0 13 33.308087805234685 37.0 33.0 39.0 18.0 39.0 14 33.916332797192574 38.0 33.0 40.0 17.0 41.0 15 34.03714541599649 38.0 33.0 40.0 17.0 41.0 16 33.996275040210556 38.0 33.0 40.0 17.0 41.0 17 33.94780121362772 38.0 33.0 40.0 17.0 41.0 18 33.93168409124141 38.0 33.0 40.0 17.0 41.0 19 33.94102756250914 39.0 33.0 40.0 18.0 41.0 20 33.94804430472291 39.0 33.0 40.0 16.0 41.0 21 33.85597675098698 39.0 33.0 40.0 11.0 41.0 22 33.79682702149437 39.0 33.0 40.0 10.0 41.0 23 33.45698201491446 39.0 33.0 40.0 2.0 41.0 24 33.5532241555783 39.0 33.0 40.0 2.0 41.0 25 33.34003509284983 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6942377334110823 1101 2 0.7681017111699369 1101 3 0.7925884447426981 1101 4 0.328647433405834 1101 5 0.5745668963394834 1101 6 0.0029999294907412377 1101 7 0.30790478844048863 1101 8 0.407333469837031 1101 9 0.7800818742898556 1101 10 0.5759434201462952 1101 11 0.344004879446139 1101 12 0.41623715549800977 1101 13 0.442303899348353 1101 14 0.7363687790250992 1101 15 0.49961565921751117 1101 16 0.3495154513192986 1101 17 0.4933574601702517 1101 18 0.7758586265835703 1101 19 0.7724866217120763 1101 20 0.5548541336688757 1101 21 0.710126495310945 1101 22 0.6237771010559925 1101 23 1.0577695399848324 1101 24 0.7045159214167498 1101 25 0.7113944362923945 1102 1 0.10199365476495714 1102 2 0.3049873172270381 1102 3 -0.001564435109667528 1102 4 -0.3642839431788616 1102 5 -0.12243924124035743 1102 6 -0.4299492076867075 1102 7 -0.2909255100207204 1102 8 -0.17273720252165958 1102 9 0.4016910579963593 1102 10 0.5787670768858391 1102 11 0.17413683510015687 1102 12 0.296653789403166 1102 13 0.1372934696252628 1102 14 0.5844393958819438 1102 15 0.23476313226441903 1102 16 0.18049381022202482 1102 17 0.35164273044114225 1102 18 0.2809069825724464 1102 19 0.3761224555580256 1102 20 0.5019909318595168 1102 21 0.3717247636531482 1102 22 0.33972093318138974 1102 23 0.5908602068858428 1102 24 0.46735419364408415 1102 25 0.4572213907669962 1103 1 0.15359361506722635 1103 2 0.011032714417005707 1103 3 0.01071350571235996 1103 4 -0.25044125016046337 1103 5 -0.21279647355241949 1103 6 -0.16935723891558752 1103 7 0.026965878879984473 1103 8 -0.012363098674288864 1103 9 0.3570476523091841 1103 10 0.5400003530355306 1103 11 0.28379472573291054 1103 12 0.2252569491534473 1103 13 0.12691771170580068 1103 14 0.35091277241018304 1103 15 0.2430878407932937 1103 16 0.5970127918807293 1103 17 0.6084705037293006 1103 18 0.39358001032778134 1103 19 0.49137583577248733 1103 20 0.3291734799812005 1103 21 0.42553172871442513 1103 22 0.15250832820688487 1103 23 0.5576464393349596 1103 24 0.17610754314006272 1103 25 0.21936346138169682 1104 1 -0.027195120441071197 1104 2 0.11482024775310151 1104 3 -0.01068377765482964 1104 4 -0.07152025728598232 1104 5 -0.04983074697520351 1104 6 -0.3911971611659091 1104 7 -0.06676670459268053 1104 8 0.02352012941139847 1104 9 0.2779023460776955 1104 10 0.28862660644974625 1104 11 -0.11232792346771703 1104 12 -0.05297221895260407 1104 13 -0.03802399906354026 1104 14 0.25702358412975457 1104 15 0.19897825427604232 1104 16 0.10207250897875042 1104 17 0.08144465932998912 1104 18 -0.12353597395647142 1104 19 -0.157509276176917 1104 20 -0.0943319804599696 1104 21 2.9900936405624634E-4 1104 22 -0.08187840275986957 1104 23 -0.009624537648740272 1104 24 -0.08847397657141443 1104 25 -0.07224850875284261 1105 1 0.9897816905784289 1105 2 0.8832167127089825 1105 3 0.7696892421256472 1105 4 0.42030378359827125 1105 5 0.47993018976247726 1105 6 0.38484727862494594 1105 7 0.4403031608715793 1105 8 0.5029655008605936 1105 9 1.07438111420349 1105 10 0.9599240888335672 1105 11 0.4594422942088414 1105 12 0.7404478047955507 1105 13 0.5746416086641872 1105 14 1.012308439446464 1105 15 0.9804595954897835 1105 16 1.144182040097519 1105 17 1.1417551675538462 1105 18 1.050213182271186 1105 19 1.1575581030828772 1105 20 1.039254725920621 1105 21 1.0738692747151433 1105 22 1.0247187274116456 1105 23 1.342503105335858 1105 24 1.0916533188792314 1105 25 1.219219978160055 1106 1 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0.606889732031398 1107 13 0.3545570237160902 1107 14 0.8982989612734045 1107 15 0.765587676088316 1107 16 0.5221299595912825 1107 17 0.6143090110705955 1107 18 0.6896525350392011 1107 19 0.6905916129187091 1107 20 0.6140653767236728 1107 21 0.579595625157026 1107 22 0.509393336873778 1107 23 0.9219704333283332 1107 24 0.7941226323874417 1107 25 0.9124045058611259 1108 1 1.4737202911672185 1108 2 1.2196305177995264 1108 3 1.2352873214929687 1108 4 1.110879864768897 1108 5 -0.18493896170461 1108 6 0.7384207634652 1108 7 1.0669182630409821 1108 8 1.1409935933725173 1108 9 1.5159093642333588 1108 10 1.4138745304269946 1108 11 1.1803313472543735 1108 12 1.3188265648576873 1108 13 1.2757464069868902 1108 14 1.7612737094539597 1108 15 1.5866136589097692 1108 16 1.4172225710959694 1108 17 1.3153388639026886 1108 18 1.4590450508686246 1108 19 1.536296438606982 1108 20 1.6646270657683218 1108 21 1.3843664882768962 1108 22 1.6514055274072987 1108 23 1.6845538804328157 1108 24 1.6110378039041606 1108 25 1.825513091909741 1109 1 1.0900630690816584 1109 2 0.22689621093636703 1109 3 0.7923618121788358 1109 4 1.0391867726562793 1109 5 -0.2916366382034816 1109 6 0.6579032868902956 1109 7 1.0377078956191923 1109 8 1.0279135566974311 1109 9 1.400606459297876 1109 10 -0.9407556996868323 1109 11 0.3433342320632775 1109 12 1.0103197330040317 1109 13 0.8856665715775378 1109 14 1.5356324544502726 1109 15 1.6317262559086743 1109 16 1.1945935933022866 1109 17 1.4069572640637062 1109 18 1.4999959196657215 1109 19 1.3837146464474017 1109 20 1.4347458125536576 1109 21 1.3977551324167123 1109 22 1.4872263561748795 1109 23 1.5561530485344335 1109 24 1.585575451778574 1109 25 1.63075718252405 1110 1 0.781751373531069 1110 2 0.09465645656197097 1110 3 0.44617277150290136 1110 4 0.7936145829745982 1110 5 1.2446312274827918 1110 6 0.6860355010973791 1110 7 1.0650992383384477 1110 8 1.107059069431589 1110 9 1.3406074811895863 1110 10 1.022839408238866 1110 11 1.054224810686236 1110 12 1.1763399757497623 1110 13 1.1593222033620876 1110 14 1.558885135754764 1110 15 1.2616086919127056 1110 16 0.8409935152669235 1110 17 1.2046366408955578 1110 18 1.187436406086178 1110 19 1.102666557152645 1110 20 1.2899344676977194 1110 21 1.1102853827337285 1110 22 1.133707564979872 1110 23 1.2958609733046202 1110 24 1.565955130997473 1110 25 1.4915476622093067 1111 1 0.008788566611062976 1111 2 0.261990568823137 1111 3 0.4412972334563321 1111 4 0.27048129510285435 1111 5 0.5471013553109287 1111 6 0.0735245881447355 1111 7 0.5689236581602799 1111 8 0.5954424756627255 1111 9 0.8517137179691687 1111 10 0.8428701599621391 1111 11 0.4899024561814116 1111 12 0.47583917438070245 1111 13 0.41558873567094423 1111 14 0.5134850810271203 1111 15 0.6087252835098411 1111 16 0.5173794059593106 1111 17 0.3929344808910429 1111 18 0.3894856522780472 1111 19 0.37222626023346095 1111 20 0.5084237241401652 1111 21 0.7466571137343294 1111 22 0.5991314218888064 1111 23 1.1606815231002656 1111 24 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0.6780894973860612 2202 20 0.7258998028547339 2202 21 0.6468482716201223 2202 22 0.5092022565053753 2202 23 0.88410048808651 2202 24 0.8945861387667975 2202 25 0.8322653993529272 2203 1 0.060082095002961466 2203 2 0.039356371626631415 2203 3 0.13930736411233013 2203 4 -0.08224344341517309 2203 5 0.09136121683596343 2203 6 0.017825244257693384 2203 7 0.2555147037482328 2203 8 0.31749534998139595 2203 9 0.5409280821968494 2203 10 0.47740617818372044 2203 11 0.3688241192407773 2203 12 0.4383738781100064 2203 13 0.4512114763873427 2203 14 0.8000285329291685 2203 15 0.5267564913695963 2203 16 0.6346389161976234 2203 17 0.7518475317596298 2203 18 0.7579219730594886 2203 19 0.6279856628193841 2203 20 0.7680654116762824 2203 21 0.6663223714768023 2203 22 0.9150696407517671 2203 23 0.9100679045069953 2203 24 0.7897454005176527 2203 25 0.7319290338552591 2204 1 0.20704560738766276 2204 2 0.34365561865116234 2204 3 0.5019630985151942 2204 4 0.140843459788087 2204 5 0.30993263694413997 2204 6 0.16292142487701256 2204 7 0.3667335050302398 2204 8 0.251522613499116 2204 9 0.8676601923646814 2204 10 0.7297068707315688 2204 11 0.6123699168556627 2204 12 0.7540738016868502 2204 13 0.5543199439548943 2204 14 1.0526064815893932 2204 15 1.0912096510821527 2204 16 1.0490905948411964 2204 17 0.8871342963348283 2204 18 1.0823092548239757 2204 19 1.248642428046253 2204 20 0.954688396681945 2204 21 0.9474695482631219 2204 22 1.1533700844800308 2204 23 1.424978714533239 2204 24 0.950825277529681 2204 25 1.0002821443963228 2205 1 0.10268202235996782 2205 2 0.313234296804346 2205 3 0.4407100657398786 2205 4 0.22708426296876638 2205 5 0.08229583237579874 2205 6 0.12023111104427642 2205 7 0.27028659898088847 2205 8 0.3124276363331653 2205 9 0.7260420336874702 2205 10 0.5340377340698197 2205 11 0.33247033632802925 2205 12 0.48744018502089403 2205 13 0.3350933796337898 2205 14 0.521420741679762 2205 15 0.4024158995021523 2205 16 0.3556413895186168 2205 17 0.5146739207686295 2205 18 0.401549279906952 2205 19 0.3075666395793917 2205 20 0.33181079221603227 2205 21 0.48768056485706524 2205 22 0.44226867679076776 2205 23 0.7980431828276764 2205 24 0.5625884512927968 2205 25 0.5985057615766323 2206 1 0.21787729955551427 2206 2 -0.0072760583674700285 2206 3 0.3442092228397904 2206 4 0.03780351634517132 2206 5 0.4618540629907031 2206 6 0.2962850566499782 2206 7 0.27422226935888006 2206 8 0.3920063785852115 2206 9 0.6685741952935302 2206 10 0.5337012150228375 2206 11 0.43363437173623964 2206 12 0.2024149621170892 2206 13 0.30993777963635694 2206 14 0.3278678056312003 2206 15 0.24401478161792767 2206 16 0.3792425920133411 2206 17 0.6751967111204635 2206 18 0.2324885205917866 2206 19 0.2344779075190644 2206 20 0.23813029942395048 2206 21 0.6073948121120338 2206 22 0.5296514559894021 2206 23 0.8500658225471796 2206 24 0.8938642110246064 2206 25 0.7500675304489377 2207 1 -1.1407683664855206 2207 2 -0.1989422412973667 2207 3 -0.18695309987068498 2207 4 0.06125829985684561 2207 5 0.04107724623663955 2207 6 -0.08523490877896478 2207 7 0.061152346821259584 2207 8 0.1408244484110064 2207 9 0.48925297472891316 2207 10 0.14706976439301656 2207 11 -0.2309264275259011 2207 12 0.06514659140702861 2207 13 -0.18266427269751517 2207 14 -4.7786252317842255 2207 15 -1.975601711870972 2207 16 -0.8296155957152251 2207 17 -0.635762184153748 2207 18 -0.21176887896833563 2207 19 -0.1350163618126743 2207 20 -0.13027717463162958 2207 21 0.1037635614267387 2207 22 0.1450968485579125 2207 23 0.3624333592950464 2207 24 0.007058615157653492 2207 25 -0.05277426182804135 2208 1 0.7567539849062186 2208 2 0.9484629710725123 2208 3 1.0251603413571857 2208 4 1.0260944318630223 2208 5 1.0444236231583517 2208 6 0.8353321707236034 2208 7 1.1036104933653448 2208 8 1.1657149075959659 2208 9 1.3790599802756063 2208 10 1.312112314351225 2208 11 1.1417972763466508 2208 12 1.2513343177702723 2208 13 1.1953591804342167 2208 14 1.4430271249262603 2208 15 1.2348116305802108 2208 16 1.0111354870292502 2208 17 0.9401565656879569 2208 18 1.1470406086592533 2208 19 0.8086017776397512 2208 20 0.9806693086649787 2208 21 1.2333921357498667 2208 22 1.2409893845860793 2208 23 1.5863873394890575 2208 24 1.3948425981376715 2208 25 1.4785430139615343 2209 1 -0.38547113363927465 2209 2 -0.3567600167105347 2209 3 0.13660947813641755 2209 4 0.30366226258966833 2209 5 0.16744568178924624 2209 6 0.3538824663232276 2209 7 0.47775906165016835 2209 8 0.48220080876003024 2209 9 0.7405320402163937 2209 10 0.2807785356536101 2209 11 0.5106047479874292 2209 12 0.4993607427176556 2209 13 0.6643408681028617 2209 14 0.5912592033802326 2209 15 0.6059288836431804 2209 16 0.627518561440013 2209 17 0.8009147328829442 2209 18 0.6300196154095801 2209 19 0.7050098273808274 2209 20 0.5889452249364879 2209 21 0.7144870769355833 2209 22 0.7174734955603768 2209 23 0.7596067556252137 2209 24 0.9000286905463923 2209 25 0.7859491599886255 2210 1 0.15120548032615133 2210 2 -0.09781595325519277 2210 3 0.5777069697646482 2210 4 0.9375510407730019 2210 5 1.1213329046946043 2210 6 0.963288981965924 2210 7 1.3039057146433493 2210 8 1.2747996296649085 2210 9 1.5692983645598986 2210 10 1.4644179833355153 2210 11 1.0167007086388935 2210 12 0.9096041923242169 2210 13 0.9608376044492246 2210 14 1.2591162247312866 2210 15 1.2303511221742198 2210 16 1.2839576552961844 2210 17 1.1777683083212196 2210 18 1.2448453027695408 2210 19 1.3519422845742355 2210 20 1.2868993053540834 2210 21 1.4494968703266267 2210 22 1.427185720531341 2210 23 1.6206596788187824 2210 24 1.32429758838893 2210 25 1.3035347367294463 2211 1 -1.389121965788771 2211 2 -0.734044241096381 2211 3 -0.08279271397981702 2211 4 0.142151342432129 2211 5 0.44519073566818435 2211 6 0.3738713786963288 2211 7 0.5298824576978873 2211 8 0.5289328462269687 2211 9 0.8059548137400654 2211 10 0.5531495553687975 2211 11 0.5071133004746358 2211 12 0.39459421811120876 2211 13 0.33734711644260784 2211 14 0.5798747514670382 2211 15 0.35381241736227054 2211 16 0.49906493890441084 2211 17 0.38439994360560803 2211 18 0.34449012347698016 2211 19 0.48299857805936597 2211 20 0.381875707825742 2211 21 0.5450692928440191 2211 22 0.6630782909590138 2211 23 0.7919144834701726 2211 24 0.5821163982950281 2211 25 0.7644333889943198 2212 1 0.9868770012018828 2212 2 -0.2062304645081312 2212 3 0.3411284955802145 2212 4 0.3123114588804583 2212 5 0.35144513769664343 2212 6 0.5076437410008765 2212 7 0.4178478988398382 2212 8 0.32489941428820046 2212 9 0.7041212221543987 2212 10 0.3370839123204803 2212 11 0.1768649086823615 2212 12 0.18550373588366398 2212 13 0.39298406061314495 2212 14 0.1842579979336776 2212 15 0.176862612225392 2212 16 -0.35166051538492127 2212 17 0.09198317129025924 2212 18 0.08620257123957487 2212 19 0.114336325159897 2212 20 0.20835596326077876 2212 21 0.2883101248860882 2212 22 0.436018141642208 2212 23 0.7428958423470107 2212 24 0.46457402143443716 2212 25 0.13076902312118932 2213 1 -0.2896124392843369 2213 2 -0.4192734431337328 2213 3 -1.1632115028875702 2213 4 -1.6596030435327833 2213 5 -2.5904251858560556 2213 6 -1.3597826488456448 2213 7 -1.1665899060070721 2213 8 -0.9259624565460918 2213 9 -1.4418286233472735 2213 10 -1.3634510860196194 2213 11 -1.1639864920173508 2213 12 -1.675958507789563 2213 13 -1.6303004972151953 2213 14 -8.442253148929112 2213 15 -4.202950457566988 2213 16 -3.0147060477018854 2213 17 -2.445009601763193 2213 18 -6.419365752772784 2213 19 -4.252598479099657 2213 20 -6.239912633996557 2213 21 -3.3433479869145195 2213 22 -3.144109032187565 2213 23 -5.6304397893085465 2213 24 -3.239200334396916 2213 25 -2.7342083767102636 2214 1 0.3491810037359926 2214 2 0.1898484548755377 2214 3 -1.4385228382207238 2214 4 -2.303358156704313 2214 5 -0.1924492442749859 2214 6 -0.37699775050306883 2214 7 -0.5138386409705582 2214 8 -0.56797227383543 2214 9 -5.321559253896012 2214 10 -1.8817827528377897 2214 11 -0.8407946609716817 2214 12 -1.0625900261363839 2214 13 -1.4529726785320456 2214 14 -2.7901124344486092 2214 15 -2.221094262675617 2214 16 -1.457244604802753 2214 17 -1.723750928309265 2214 18 -1.037397446861199 2214 19 -4.583595661174016 2214 20 -2.0773365045537773 2214 21 -4.013243147960484 2214 22 -5.595245292527533 2214 23 -6.085802938113201 2214 24 -5.26278303899953 2214 25 -3.2833993593482056 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21912.0 3 13.0 4 376.0 5 2632.0 6 3353.0 7 2605.0 8 1993.0 9 1717.0 10 1430.0 11 1234.0 12 1220.0 13 1097.0 14 1139.0 15 1164.0 16 1206.0 17 1306.0 18 1438.0 19 1676.0 20 2175.0 21 2861.0 22 3648.0 23 4293.0 24 5095.0 25 5914.0 26 6907.0 27 7599.0 28 8576.0 29 10652.0 30 12780.0 31 15764.0 32 19950.0 33 25790.0 34 33341.0 35 44527.0 36 60449.0 37 89334.0 38 139954.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.40162809841764 19.927741699442056 9.563157413335773 29.10747278880454 2 11.464873819284202 21.3861777171548 42.26734002055167 24.88160844300932 3 19.966761069130758 25.756880440406693 28.232796842118734 26.04356164834381 4 11.689377830515541 18.272171952733476 36.933051279754125 33.10539893699686 5 11.92206861501223 39.76332464341474 35.35815749350084 12.956449248072191 6 32.20711256764022 36.8236207266702 17.760133876718186 13.209132828971393 7 27.0583796693113 31.61542237166949 21.52718255276441 19.799015406254806 8 23.926984970187668 41.29342480056419 19.786047278422465 14.993542950825677 9 26.12893830064354 16.208423324155643 20.061038049859953 37.60160032534087 10 15.92823128319029 30.963283884943376 33.037374208231654 20.071110623634684 11 32.58375007311224 23.47231970521144 24.26449084634731 19.679439375329004 12 25.18703333059158 25.833721748508026 31.434394392198794 17.5448505287016 13 30.491117122386314 22.90996490715017 24.700614124872057 21.89830384559146 14 19.833059855889275 25.792672999635975 26.456153059563203 27.918114084911544 15 24.253989142951145 32.979291184588114 22.05132423095904 20.71539544150171 16 19.233342228688617 28.883827878530877 32.89624238259045 18.986587510190056 17 20.650860642927775 31.128459433622556 29.44793462147979 18.772745301969874 18 20.71047989837231 27.835750646597024 33.32163518218956 18.132134272841096 19 23.617640392756094 27.649385496099487 27.72926003640963 21.003714074734788 20 23.31286213010181 28.920875143938147 28.834055308804444 18.9322074171556 21 20.325339961982746 28.286664717063896 27.7781839450212 23.609811375932154 22 21.791197543500513 31.333528293610176 25.682482819125603 21.19279134376371 23 19.331773651118585 30.875859043719846 28.570514695130868 21.221852610030705 24 22.878934930060918 29.148868893238948 29.094036215156116 18.878159961544014 25 21.467319783594093 28.559730954817958 28.11247989472145 21.8604693668665 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 539.0 1 539.0 2 536.0 3 533.0 4 533.0 5 533.0 6 587.0 7 641.0 8 641.0 9 641.0 10 892.0 11 1143.0 12 1143.0 13 1143.0 14 1636.5 15 2130.0 16 2130.0 17 2130.0 18 3545.0 19 4960.0 20 4960.0 21 4960.0 22 8700.0 23 12440.0 24 12440.0 25 12440.0 26 19324.5 27 26209.0 28 26209.0 29 26209.0 30 37263.0 31 48317.0 32 48317.0 33 48317.0 34 53440.0 35 58563.0 36 58563.0 37 58563.0 38 61378.0 39 64193.0 40 64193.0 41 64193.0 42 66760.5 43 69328.0 44 69328.0 45 69328.0 46 81455.0 47 93582.0 48 93582.0 49 93582.0 50 83571.5 51 73561.0 52 73561.0 53 73561.0 54 56843.0 55 40125.0 56 40125.0 57 40125.0 58 32076.0 59 24027.0 60 24027.0 61 24027.0 62 19056.0 63 14085.0 64 14085.0 65 14085.0 66 10762.5 67 7440.0 68 7440.0 69 7440.0 70 5390.5 71 3341.0 72 3341.0 73 3341.0 74 2301.0 75 1261.0 76 1261.0 77 1261.0 78 864.0 79 467.0 80 467.0 81 467.0 82 321.5 83 176.0 84 176.0 85 176.0 86 111.5 87 47.0 88 47.0 89 47.0 90 27.5 91 8.0 92 8.0 93 8.0 94 6.0 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08590437198420822 2 0.03765170346541892 3 0.031254569381488524 4 0.03271677145781547 5 0.02302968270214944 6 0.39132183067699955 7 0.20251498757128233 8 0.22024418774674662 9 0.22499634449480915 10 0.015535897060973828 11 0.0029244041526538967 12 0.0045693814885217135 13 0.0 14 0.08352829361017693 15 0.0018277525954086854 16 0.003289954671735634 17 0.005300482526685188 18 0.0045693814885217135 19 0.0021933031144904225 20 0.0018277525954086854 21 0.0 22 0.0 23 0.0 24 1.8277525954086854E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 547120.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.69767178684413 #Duplication Level Percentage of deduplicated Percentage of total 1 92.53788803630215 63.57137460165616 2 5.062938391561094 6.95624159800953 3 0.9770064489303487 2.013542050867416 4 0.3949082153562055 1.0851709985787585 5 0.23021765387080956 0.7907708412577079 6 0.14331641390754646 0.5907302378572877 7 0.10697558143507534 0.5144281367843536 8 0.06950477247932839 0.38198528379233426 9 0.05307139726076973 0.328129328726062 >10 0.33543799990839557 4.3830517274448 >50 0.04116025378394466 2.0105434022500988 >100 0.03287474567490384 4.519223923270906 >500 0.009087328072737497 4.028431027366141 >1k 0.005078212746529778 6.258573381896185 >5k 5.345487101610292E-4 2.5678034602422737 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 7248 1.3247550811522153 No Hit GTACATGGGAAGCAGTGGTATCAAC 6737 1.2313569235268313 No Hit GAGTACATGGGAAGCAGTGGTATCA 3152 0.5761076180728176 No Hit CTGTAGGACGTGGAATATGGCAAGA 2921 0.533886533118877 No Hit GTCCTACAGTGGACATTTCTAAATT 2784 0.508846322561778 No Hit CATGTACTCTGCGTTGATACCACTG 2594 0.47411902324901295 No Hit GCGTTGATACCACTGCTTCCCATGT 2117 0.38693522444801876 No Hit ACGCAGAGTACATGGGAAGCAGTGG 1982 0.3622605644100015 No Hit GTATCAACGCAGAGTACATGGGAAG 1941 0.35476677876882584 No Hit CTTTAGGACGTGAAATATGGCGAGG 1886 0.3447141394940781 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1863 0.3405103085246381 No Hit GTATCAACGCAGAGTACTTTTTTTT 1575 0.28787103377686796 No Hit ACTCTGCGTTGATACCACTGCTTCC 1510 0.2759906419067115 No Hit GTCCTACAGTGTGCATTTCTCATTT 1488 0.2719695861968124 No Hit GCTTCCCATGTACTCTGCGTTGATA 1480 0.2705073841204855 No Hit CTGTAGGACCTGGAATATGGCGAGA 1256 0.2295657259833309 No Hit CTGAAGGACCTGGAATATGGCGAGA 1179 0.21549203099868403 No Hit TATCAACGCAGAGTACATGGGAAGC 1155 0.21110542476970315 No Hit GGTATCAACGCAGAGTACATGGGAA 1150 0.21019154847199883 No Hit GATATACACTGTTCTACAAATCCCG 1029 0.18807574206755373 No Hit CAGTGGTATCAACGCAGAGTACATG 1024 0.1871618657698494 No Hit GGTATCAACGCAGAGTACTTTTTTT 990 0.18094750694545986 No Hit GTGTATATCAATGAGTTACAATGAA 850 0.15535897060973825 No Hit GTGGTATCAACGCAGAGTACATGGG 792 0.1447580055563679 No Hit TATCAACGCAGAGTACTTTTTTTTT 791 0.14457523029682703 No Hit CATGGGAAGCAGTGGTATCAACGCA 784 0.14329580348004092 No Hit ACATGGGAAGCAGTGGTATCAACGC 764 0.13964029828922356 No Hit GCAGTGGTATCAACGCAGAGTACAT 729 0.13324316420529317 No Hit GTGTATATCAATGAGTTACAATGAG 719 0.13141541160988449 No Hit ATACCACTGCTTCCCATGTACTCTG 711 0.12995320953355755 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 703 0.12849100745723058 No Hit ACCTGGAATATGGCGAGAAAACTGA 678 0.12392162596870887 No Hit GTTCTACAGTGTGGTTTTTATCATT 669 0.12227664863284106 No Hit GGGAAGCAGTGGTATCAACGCAGAG 647 0.11825559292294197 No Hit GTCCTTCAGTGTGCATTTCTCATTT 646 0.11807281766340108 No Hit GATATACACTGTTCTACAATGCCGG 643 0.11752449188477847 No Hit CTGTAGAACATATTAGATGAGTGAG 642 0.11734171662523762 No Hit CTGCTTCCCATGTACTCTGCGTTGA 621 0.11350343617487937 No Hit GAATATGGCAAGAAAACTGAAAATC 613 0.11204123409855242 No Hit ATTCCAGGTCCTTCAGTGTGCATTT 610 0.1114929083199298 No Hit CCATGTACTCTGCGTTGATACCACT 603 0.11021348150314374 No Hit CTGTAGGACATGGAATATGGCAAGA 596 0.10893405468635767 No Hit GACCTGGAATATGGCGAGAAAACTG 594 0.10856850416727591 No Hit ACAGTGGACATTTCTAAATTTTCCA 593 0.10838572890773505 No Hit AAGCAGTGGTATCAACGCAGAGTAC 588 0.10747185261003071 No Hit GCCATATTCCACGTCCTACAGTGGA 584 0.10674075157186723 No Hit CCACTGCTTCCCATGTACTCTGCGT 557 0.10180581956426378 No Hit GATACCACTGCTTCCCATGTACTCT 556 0.10162304430472291 No Hit CTTCCCATGTACTCTGCGTTGATAC 555 0.10144026904518205 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 1.8277525954086854E-4 0.0 0.0 0.0 0.0 9 1.8277525954086854E-4 0.0 0.0 0.0 0.0 10 1.8277525954086854E-4 0.0 0.0 0.0 0.0 11 1.8277525954086854E-4 0.0 0.0 0.0 0.0 12 1.8277525954086854E-4 0.0 0.0 0.0 0.0 13 1.8277525954086854E-4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGACAG 60 7.393464E-8 17.470331 5 GTCCTAG 85 9.749783E-10 15.690945 1 TAGAACC 70 4.269241E-7 14.974569 4 TGTCGAG 45 6.877584E-4 14.742021 18 GATTTCA 40 0.0053587477 14.21552 16 TAGGACC 405 0.0 14.117439 4 TCCAACG 125 7.2759576E-12 13.646899 18 ACTGTTC 525 0.0 13.579549 8 TGAAGTG 50 0.0014630478 13.34098 5 ATGTCGA 50 0.001524966 13.267819 17 GATATAC 580 0.0 13.1402 1 CTTAAGG 60 3.9890627E-4 12.702192 1 GTTCAGG 60 3.9890627E-4 12.702192 1 CTCTAGG 60 3.9890627E-4 12.702192 1 CACTGTT 575 0.0 12.56173 7 TAGGACA 275 0.0 12.474683 4 GTCCTAA 555 0.0 12.358891 1 AATCCCG 315 0.0 12.334033 19 TAGGACT 155 1.8189894E-12 12.295834 4 TATACAC 680 0.0 12.192982 3 >>END_MODULE