FastQCFastQC Report
Thu 26 May 2016
SRR1926526_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926526_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences713923
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC14600.2045038470535338No Hit
CCCATGTACTCTGCGTTGATACCAC13510.1892360940885782No Hit
GTCCTACAGTGGACATTTCTAAATT10130.14189205278440392No Hit
CTGTAGGACGTGGAATATGGCAAGA8240.1154186095699396No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTTAC350.0058068119.1214451
TGTAGGA6300.017.6468622
CCTGATG452.2504017E-417.0726073
TAGGACC2550.016.0086364
GATATAC2550.015.7470721
TAGGACA1700.015.1660754
CTGTAGG6100.015.1404231
GTAGGAC7250.015.138273
TTTAGAC450.00441978914.6336623
CTTATAC450.00441978914.6336623
GTCCTAA2750.014.6018311
GTCTTAG550.00448233314.60183051
GTCCTAG751.4807998E-414.2773441
GTCCAAT751.4807998E-414.2773441
ATACACT3400.013.5862754
AGGACCT5000.013.5790015
ACTGTTC2900.013.4643028
GTCTTAA700.001426041113.3850111
TCCAACG1400.013.37566418
TCCTAAA3200.013.2964212
Produced by FastQC (version 0.11.5)