FastQCFastQC Report
Thu 26 May 2016
SRR1926526_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926526_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences713923
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC38430.5382933453607742No Hit
GTACATGGGAAGCAGTGGTATCAAC38090.533530927004733No Hit
CTGTAGGACGTGGAATATGGCAAGA24040.33673099199773643No Hit
GTCCTACAGTGGACATTTCTAAATT22270.3119384023206984No Hit
GTATCAACGCAGAGTACTTTTTTTT16530.23153757478047354No Hit
GAGTACATGGGAAGCAGTGGTATCA16470.2306971480117604No Hit
GTCCTAAAGTGTGTATTTCTCATTT16000.224113804990174No Hit
CTTTAGGACGTGAAATATGGCGAGG14840.20786555412838642No Hit
GTCCTACAGTGTGCATTTCTCATTT14820.20758541187214868No Hit
CATGTACTCTGCGTTGATACCACTG13810.19343822793214394No Hit
CTGTAGGACCTGGAATATGGCGAGA12150.1701864206644134No Hit
GCGTTGATACCACTGCTTCCCATGT11020.15435838318698236No Hit
ACGCAGAGTACATGGGAAGCAGTGG10800.15127681836836745No Hit
GGTATCAACGCAGAGTACTTTTTTT10620.148755538062228No Hit
GTATCAACGCAGAGTACATGGGAAG10490.14693461339668284No Hit
GATATACACTGTTCTACAAATCCCG10270.14385304857806794No Hit
CTGAAGGACCTGGAATATGGCGAGA9490.13292750058479696No Hit
GTGTATATCAATGAGTTACAATGAA7470.10463313270478748No Hit
TATCAACGCAGAGTACTTTTTTTTT7140.10001078547686515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1250.016.67383619
TCCAACG1400.016.2407518
GGGACGC400.005149861314.3018076
TAGGACC4450.013.92783454
CCCTTAG551.8938986E-413.8723311
GGACTGT801.9153758E-613.109996
ACTGTTC4050.012.9035718
GGCGAGG4700.012.90045419
TGTAGGA12050.012.7489792
CCTGGAC1501.8189894E-1212.7163043
AGAGAGT603.9639208E-412.7127176
GTAGGAC11750.012.4998563
CTTATAC701.05023326E-412.262153
GTCTTAT701.05023326E-412.262151
GATATAC4700.011.9722651
CTGTAGG12350.011.96981
AATGCCG1900.011.96686818
TAGGACG11250.011.9507984
GGACGTG10800.011.9181746
AGGACGT10900.011.8971435
Produced by FastQC (version 0.11.5)