FastQCFastQC Report
Thu 26 May 2016
SRR1926525_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926525_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578837
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC22560.3897470272287362No Hit
GTACATGGGAAGCAGTGGTATCAAC22110.3819728179090141No Hit
GAGTACATGGGAAGCAGTGGTATCA10840.18727206450175093No Hit
GTCCTACAGTGGACATTTCTAAATT9210.15911215074364632No Hit
CATGTACTCTGCGTTGATACCACTG8480.14650065562498596No Hit
GCGTTGATACCACTGCTTCCCATGT7130.12317802766581958No Hit
ACGCAGAGTACATGGGAAGCAGTGG6440.11125757337557896No Hit
CTGTAGGACGTGGAATATGGCAAGA6250.10797512944058518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC5000.017.1082121
CTAGGAC852.584784E-916.7560753
CTCTAGG654.4170636E-516.4502051
GATATAC1750.016.038951
TGTAGGA5200.015.816142
CTGTAGG5100.015.724461
TCTAGGA801.2117502E-515.4207362
TCCTACA6000.015.3065082
TAAGACC505.9860706E-415.0128284
TAGGACC2750.014.8178574
GTATTAA550.004522899614.5808621
GTCTTAG550.004522899614.5808621
CCAACGA553.218353E-513.90901119
GCCATAT1253.0426236E-813.9004211
TAAAACA851.0068816E-613.8774044
TGCAATA400.006524867413.79889611
GTAGGAC5800.013.4115273
GTGTAGG600.00805050313.365791
GTGTAGC802.5890395E-413.365791
TAGACAG550.001505318613.2894685
Produced by FastQC (version 0.11.5)