##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926525_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 578837 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 19.974899669509725 26.0 2.0 30.0 2.0 31.0 2 15.514749402681584 12.0 2.0 30.0 2.0 31.0 3 18.82833682021018 16.0 7.0 30.0 2.0 31.0 4 20.855116379913515 27.0 2.0 35.0 2.0 35.0 5 21.08062373345173 27.0 2.0 35.0 2.0 35.0 6 21.97658926433521 29.0 2.0 35.0 2.0 36.0 7 21.68640567206312 28.0 2.0 35.0 2.0 37.0 8 21.93128635522608 29.0 2.0 35.0 2.0 37.0 9 24.573049062171215 31.0 10.0 37.0 2.0 39.0 10 25.222724186601756 31.0 8.0 37.0 2.0 39.0 11 22.512173547993648 29.0 2.0 37.0 2.0 39.0 12 23.579024837734977 30.0 2.0 37.0 2.0 39.0 13 20.419527431729485 25.0 2.0 35.0 2.0 39.0 14 23.573966418870942 30.0 2.0 37.0 2.0 40.0 15 24.604272014401293 30.0 2.0 38.0 2.0 40.0 16 24.940803715035493 31.0 2.0 38.0 2.0 40.0 17 24.282960488013032 30.0 2.0 38.0 2.0 40.0 18 24.352427367289927 30.0 2.0 37.0 2.0 40.0 19 24.684458664529046 30.0 2.0 38.0 2.0 40.0 20 24.932300457641787 31.0 2.0 38.0 2.0 40.0 21 22.854318227756693 30.0 2.0 37.0 2.0 40.0 22 23.754737516779336 30.0 2.0 37.0 2.0 40.0 23 23.719705201982595 30.0 2.0 37.0 2.0 40.0 24 22.86686061879251 30.0 2.0 38.0 2.0 40.0 25 22.657079626907056 30.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.339999954971724 1101 2 9.58307557453001 1101 3 6.500987707084796 1101 4 7.889585589118621 1101 5 7.2209017286478385 1101 6 7.277755705002587 1101 7 6.714585255993711 1101 8 5.057928310073109 1101 9 5.170769142040367 1101 10 4.876785605124947 1101 11 7.517191320259329 1101 12 5.310796192895683 1101 13 9.609577109115019 1101 14 7.1723713175056325 1101 15 6.5175231683695145 1101 16 5.952576646130694 1101 17 2.3243710467099667 1101 18 4.488904611330227 1101 19 5.155230084067121 1101 20 2.682763489689407 1101 21 0.385597594962924 1101 22 4.052105731853555 1101 23 5.223368556716583 1101 24 6.430249977469135 1101 25 7.019883969015289 1102 1 5.497467626725108 1102 2 10.078762077359372 1102 3 6.932049381003612 1102 4 8.06405802073391 1102 5 7.6835214912676015 1102 6 6.611787696177437 1102 7 6.6850158907100585 1102 8 6.5096432903595165 1102 9 6.028981435966944 1102 10 5.461935040274383 1102 11 7.986544346755213 1102 12 5.267926792883426 1102 13 9.659456580423061 1102 14 7.37433654804229 1102 15 5.37138110794174 1102 16 5.719997127836891 1102 17 6.45085671605278 1102 18 6.40332209003342 1102 19 5.4928452502537155 1102 20 5.801978623190255 1102 21 5.044950825744728 1102 22 5.943297944474338 1102 23 6.432583894503349 1102 24 7.650253654615671 1102 25 7.616164943867691 1103 1 4.606393621365388 1103 2 8.807900399354835 1103 3 5.8658980719951614 1103 4 7.482878882411914 1103 5 6.581789889535997 1103 6 6.431618658865542 1103 7 6.451012592421048 1103 8 6.077711729856528 1103 9 5.199925571196797 1103 10 4.4781688065081475 1103 11 5.31690921997723 1103 12 4.2843151664146575 1103 13 8.325948025485935 1103 14 6.5353342804685965 1103 15 5.663032313878659 1103 16 5.382169875723925 1103 17 3.3464658688047884 1103 18 2.367009989435605 1103 19 3.930177759014793 1103 20 4.491135498061418 1103 21 6.60141341077874 1103 22 2.4278269075747296 1103 23 4.51955565290368 1103 24 6.02405287127203 1103 25 2.2239880731193935 1104 1 5.348730028919189 1104 2 9.561282041866914 1104 3 6.4990776943052175 1104 4 7.154003168753594 1104 5 7.200630469246143 1104 6 6.732167626805815 1104 7 6.6507051703744935 1104 8 6.662055164199963 1104 9 5.4967195984256065 1104 10 4.528761691883641 1104 11 7.346421736446267 1104 12 4.652233479985146 1104 13 8.562070115955851 1104 14 6.3063926906574395 1104 15 5.557301088582218 1104 16 5.147035927546497 1104 17 5.583554142849582 1104 18 2.8246296644465865 1104 19 3.691728044478122 1104 20 3.291157456779029 1104 21 2.151347534625909 1104 22 4.388345133310349 1104 23 4.62598955716367 1104 24 5.312304957350204 1104 25 4.795595451248509 1105 1 3.56961028100352 1105 2 9.267630905208602 1105 3 6.221240763321948 1105 4 5.598496289082998 1105 5 2.2908205540338606 1105 6 4.775848230928116 1105 7 5.75705914140994 1105 8 2.9370867926113142 1105 9 3.8494687163661823 1105 10 3.3122190504987508 1105 11 6.658670771877947 1105 12 4.184610364920591 1105 13 5.126205069082939 1105 14 3.8355772669938872 1105 15 4.401510535458275 1105 16 4.6073059280965545 1105 17 5.094294051225415 1105 18 2.154547391287121 1105 19 2.430314313425704 1105 20 1.6650897523337633 1105 21 2.6838521119053134 1105 22 4.0391006790870705 1105 23 1.7511378767324253 1105 24 4.693553041965043 1105 25 5.01431279147592 1106 1 6.404820156778225 1106 2 10.598485309117827 1106 3 7.673089358638503 1106 4 9.138086536249705 1106 5 8.475693702617896 1106 6 7.486011906409473 1106 7 7.241389947181961 1106 8 7.0073229101526415 1106 9 6.37691266325238 1106 10 5.616240324031722 1106 11 7.797950553073356 1106 12 5.209358159950803 1106 13 4.60003527765538 1106 14 5.561840042590795 1106 15 5.7724434506870566 1106 16 5.740265509162022 1106 17 6.299137398188645 1106 18 6.2549994733155 1106 19 6.016552267307112 1106 20 5.431444656178794 1106 21 5.53083284978722 1106 22 6.095031240532489 1106 23 6.476447467329741 1106 24 7.374380017489589 1106 25 6.59593849712461 1107 1 3.785062664740316 1107 2 -13.03654404508961 1107 3 -5.077714524558612 1107 4 1.8479115621504754 1107 5 4.3660144884664795 1107 6 5.078974570927336 1107 7 4.859865830686051 1107 8 5.371948212328306 1107 9 3.5583331766632256 1107 10 3.2504504464368473 1107 11 5.543647187891668 1107 12 3.1717483214948707 1107 13 4.586227288706375 1107 14 4.1229159798150015 1107 15 3.7821053976576273 1107 16 3.476079560810078 1107 17 4.166162838501759 1107 18 -0.6503993985620191 1107 19 1.855430284267321 1107 20 2.3696857060233896 1107 21 -1.127017961770278 1107 22 2.0695084286680157 1107 23 -1.257212023863996 1107 24 2.463238848693301 1107 25 3.2465370927272375 1108 1 5.822815783312095 1108 2 10.69974084422704 1108 3 0.6003941395037415 1108 4 6.660047099714877 1108 5 7.763392246114293 1108 6 7.90421565870027 1108 7 7.964021410145936 1108 8 7.656214758215189 1108 9 6.159476810247554 1108 10 5.534814182306555 1108 11 7.506622522395055 1108 12 3.014533616556111 1108 13 3.8427833663828714 1108 14 5.4135932902001755 1108 15 5.433878407150164 1108 16 5.363453355371213 1108 17 0.6705420492813552 1108 18 4.128732119300182 1108 19 5.133421942913454 1108 20 5.573841285483276 1108 21 5.185372345652016 1108 22 2.7184987851570988 1108 23 5.3790728654526525 1108 24 5.853777451044731 1108 25 6.433580630065176 1109 1 3.8614377062823557 1109 2 -13.03654404508961 1109 3 -4.870320041341596 1109 4 2.535162309907207 1109 5 1.1493810554322508 1109 6 2.996663171791411 1109 7 4.355765631284253 1109 8 4.7719357497161425 1109 9 4.04418811185764 1109 10 3.6192897618240174 1109 11 1.9630057787856536 1109 12 2.5503333995939066 1109 13 3.3698638789356856 1109 14 4.315654431127729 1109 15 4.3247233770527735 1109 16 4.126582219500673 1109 17 4.787773007993284 1109 18 4.966051249493816 1109 19 3.59024666844811 1109 20 2.234653303231763 1109 21 1.2063569136035994 1109 22 3.717597494802945 1109 23 4.2052879761360025 1109 24 5.248113391730854 1109 25 5.435781858430129 1110 1 3.1297897447615135 1110 2 8.567406058860495 1110 3 5.877248583345672 1110 4 7.603414802947835 1110 5 8.106479614225723 1110 6 3.5293149565618407 1110 7 6.312452353860806 1110 8 7.296859549004349 1110 9 5.665340636611862 1110 10 5.472612200951541 1110 11 2.853373656441665 1110 12 5.7043050864045775 1110 13 9.85312835266626 1110 14 7.755107900242216 1110 15 6.939445090291436 1110 16 6.5509827445367925 1110 17 7.0641877865267055 1110 18 7.016740139165755 1110 19 5.947599376436411 1110 20 6.13536994229586 1110 21 3.876742586107916 1110 22 5.861145540893364 1110 23 6.559627892975918 1110 24 7.359910407129565 1110 25 7.902920852052173 1111 1 5.338566838687122 1111 2 9.444644073722273 1111 3 7.533564838671829 1111 4 8.095640158539528 1111 5 8.636845395086553 1111 6 7.836439140913747 1111 7 7.480075498711674 1111 8 7.694953454028944 1111 9 6.394034982137889 1111 10 5.562147204347934 1111 11 7.509498478903122 1111 12 6.893346069504965 1111 13 8.484683899073293 1111 14 6.634894236464195 1111 15 6.309206931340409 1111 16 5.921633822118562 1111 17 7.179529175135421 1111 18 6.887632051641827 1111 19 5.857080449283821 1111 20 5.502766661177727 1111 21 8.117915060943755 1111 22 7.383942519135886 1111 23 7.4111048078191715 1111 24 7.3831087768625885 1111 25 7.701545828894972 1112 1 -1.4818218668500975 1112 2 7.371892168902161 1112 3 5.722987484640129 1112 4 -0.22349971841113003 1112 5 4.000570286094174 1112 6 2.9341065002422724 1112 7 5.655109113184235 1112 8 5.809735672991582 1112 9 4.609652469812584 1112 10 3.698913288363741 1112 11 0.5194157669282227 1112 12 4.380087706631642 1112 13 7.97750058814961 1112 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3.6753966771709834 2204 8 3.5860204265798608 2204 9 2.131922368437497 2204 10 1.7727364340026064 2204 11 3.03839850305188 2204 12 3.2806196749142877 2204 13 1.6898738692898263 2204 14 2.5482746598723907 2204 15 2.6537935851277368 2204 16 2.7261154288158167 2204 17 -2.041357486701493 2204 18 1.3072046186546231 2204 19 2.0546172761372397 2204 20 2.4244441947847477 2204 21 3.898567882323455 2204 22 3.3271124458846586 2204 23 1.8328286265425078 2204 24 3.314506184896075 2204 25 3.271345796696629 2205 1 5.555322213646583 2205 2 9.493321080151237 2205 3 6.663031253521407 2205 4 6.850830070594316 2205 5 7.289742245465234 2205 6 6.845765911741118 2205 7 7.053756797477391 2205 8 6.632233829465335 2205 9 5.486362191737463 2205 10 5.31358656793747 2205 11 7.257599358355229 2205 12 6.477080974787402 2205 13 9.451358196560847 2205 14 7.134692019710727 2205 15 5.954677085292218 2205 16 5.6937889567071664 2205 17 6.501519293222376 2205 18 6.140408716591558 2205 19 4.320748203342465 2205 20 4.853364726764635 2205 21 -1.0779700188937085 2205 22 3.3977924590431705 2205 23 4.744907436636964 2205 24 6.568900188373682 2205 25 7.1037568592465625 2206 1 3.586150730024233 2206 2 7.998138035835247 2206 3 5.43952450978345 2206 4 1.6937973152956651 2206 5 4.896678661650206 2206 6 5.19644028553688 2206 7 5.451637336976425 2206 8 5.269998762027953 2206 9 4.1780770279609705 2206 10 2.9773630611937314 2206 11 2.646809377623054 2206 12 3.7651349246910613 2206 13 6.724961279412483 2206 14 -0.3123214677651873 2206 15 1.9469699561978047 2206 16 2.588779322795798 2206 17 3.583407539850505 2206 18 -1.6746201851425475 2206 19 0.35254011085691417 2206 20 1.7127096207916104 2206 21 2.3950550292758486 2206 22 -2.493344342498254 2206 23 -2.983416262784999 2206 24 0.560229668142469 2206 25 1.7270709247571574 2207 1 1.2411812567554357 2207 2 -13.03654404508961 2207 3 -5.880785632519869 2207 4 -4.42689354061352 2207 5 0.7705266547787879 2207 6 1.4565791067175553 2207 7 2.5998138580897816 2207 8 2.8877762302824763 2207 9 0.720924719304378 2207 10 0.24638713436502968 2207 11 -1.5655661359921034 2207 12 -1.6849254323440128 2207 13 0.36299317578409784 2207 14 -5.16319316263716 2207 15 -1.321816170969825 2207 16 -0.16245103214999546 2207 17 0.8762029991443292 2207 18 0.8287052553477388 2207 19 0.32631381840386453 2207 20 0.3281076290094518 2207 21 -3.1650524526750736 2207 22 -3.4877322989483304 2207 23 -0.9054047949483355 2207 24 0.6557736939567071 2207 25 1.0767993180993116 2208 1 4.966882540979785 2208 2 6.4653570955948005 2208 3 6.491815036315167 2208 4 8.093102794536968 2208 5 3.609811551017735 2208 6 6.123532089752356 2208 7 6.486940310668157 2208 8 6.648228686495159 2208 9 5.120511660223951 2208 10 4.720408695896328 2208 11 7.063009001057999 2208 12 6.298170449281347 2208 13 8.663022189176068 2208 14 3.3931500810600603 2208 15 4.870515894936386 2208 16 5.015023123025841 2208 17 5.890877667399096 2208 18 1.1272454236254106 2208 19 3.5357217925055977 2208 20 4.484592885625268 2208 21 6.846533816355418 2208 22 5.783718523352277 2208 23 1.9460199152995017 2208 24 3.1219654572074305 2208 25 4.360046374767048 2209 1 2.851044008734224 2209 2 4.416854013162819 2209 3 4.854914449361054 2209 4 0.5632456569534483 2209 5 4.627626900421554 2209 6 5.005150089678526 2209 7 5.482767030000726 2209 8 4.1571566646412705 2209 9 3.6815770373142804 2209 10 2.917621586737628 2209 11 -0.7529642722263574 2209 12 2.6282398501451674 2209 13 1.640167894074736 2209 14 3.1422382718386075 2209 15 3.5409670005512126 2209 16 3.2246137317600123 2209 17 1.1025706054920477 2209 18 2.922987237645863 2209 19 3.4839092486686134 2209 20 3.4430723422312717 2209 21 5.481348685859647 2209 22 4.224286650636415 2209 23 -0.11449357726205633 2209 24 -3.1678957410649318 2209 25 -0.6957530272042298 2210 1 -2.455464220860552 2210 2 -13.03654404508961 2210 3 -7.450672542121465 2210 4 -2.5526357733481397 2210 5 -1.129829370549519 2210 6 -2.0149265478023644 2210 7 -1.631208390413434 2210 8 -19.4515811994364 2210 9 -12.711966354192063 2210 10 -7.532353595425295 2210 11 -9.701607232895052 2210 12 -5.044184554723103 2210 13 -5.439689949354495 2210 14 -4.5572355900153845 2210 15 -6.323861160744876 2210 16 -5.7795020583406504 2210 17 -5.038233889514601 2210 18 -5.11018527610322 2210 19 -9.963588120640656 2210 20 -7.193167656180389 2210 21 -5.267911742595494 2210 22 -9.374019780161527 2210 23 -7.507518772330256 2210 24 -9.875116738940525 2210 25 -7.688895362463429 2211 1 -0.4817475698851297 2211 2 4.136976516595446 2211 3 0.25307981925714884 2211 4 1.144875984830282 2211 5 0.3699665407016184 2211 6 -0.8923492043199737 2211 7 -19.181310639902186 2211 8 -12.91095933383961 2211 9 -9.457478965646056 2211 10 -7.903138752532609 2211 11 -9.782659182198996 2211 12 -5.5248699647363715 2211 13 -6.623186246015438 2211 14 -5.33061913533437 2211 15 -5.867285913530839 2211 16 -11.559003138267546 2211 17 -8.03629453361459 2211 18 -6.492077373663793 2211 19 -9.254064867855716 2211 20 -7.30769939044248 2211 21 -5.128242222788629 2211 22 -6.651123073682172 2211 23 -8.606961271607227 2211 24 -20.443623896404738 2211 25 -20.20934526021394 2212 1 -1.713623301792893 2212 2 -13.03654404508961 2212 3 -7.699652723922128 2212 4 -18.37267667314364 2212 5 -18.58790708016097 2212 6 -19.424946997700115 2212 7 -9.553038388593286 2212 8 -9.452628319855249 2212 9 -5.7632559825606755 2212 10 -5.895843568551346 2212 11 -5.608380324684042 2212 12 -3.3776870547498667 2212 13 -5.627523866205852 2212 14 -4.023013596203889 2212 15 -4.074521232051847 2212 16 -4.321759470090239 2212 17 -4.396926166733845 2212 18 -3.625083722553395 2212 19 -4.0766814085773575 2212 20 -4.26677872268003 2212 21 -2.4170857166209103 2212 22 -3.5160833996863623 2212 23 -3.6441230186283953 2212 24 -2.7755610691796058 2212 25 -3.011439501051637 2213 1 -17.518858903887136 2213 2 -13.03654404508961 2213 3 -8.020973017016237 2213 4 -5.200262880040192 2213 5 -18.58790708016097 2213 6 -9.938621195084181 2213 7 -19.181310639902186 2213 8 -9.78451817922237 2213 9 -7.781318498513386 2213 10 -6.597866216732584 2213 11 -5.83729753244593 2213 12 -4.580492241793465 2213 13 -17.85789035835245 2213 14 -11.319943827603806 2213 15 -6.5784550928881735 2213 16 -5.567707014462123 2213 17 -4.291684961736053 2213 18 -4.498115702681762 2213 19 -5.110285133885672 2213 20 -5.044736900117758 2213 21 -3.2417098551744914 2213 22 -4.321747312629693 2213 23 -3.879209645742712 2213 24 -3.086905703776914 2213 25 -3.956075343448184 2214 1 -17.518858903887136 2214 2 -13.03654404508961 2214 3 -11.719960513863425 2214 4 -18.37267667314364 2214 5 -18.58790708016097 2214 6 -19.424946997700115 2214 7 -15.386159124750671 2214 8 -14.094005441860643 2214 9 -13.284518873247649 2214 10 -13.36910392076458 2214 11 -13.666727143659134 2214 12 -11.787199985100495 2214 13 -17.85789035835245 2214 14 -15.793554608420294 2214 15 -22.155149504303157 2214 16 -17.22065840710436 2214 17 -21.883837161498242 2214 18 -16.743091020665403 2214 19 -14.147600018762983 2214 20 -13.009066522140603 2214 21 -10.620404771039441 2214 22 -11.595607435859613 2214 23 -11.55963626628824 2214 24 -10.547866320647161 2214 25 -10.271163442032123 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 129163.0 3 6.0 4 686.0 5 2265.0 6 2919.0 7 2721.0 8 2313.0 9 2273.0 10 2098.0 11 1974.0 12 1804.0 13 1975.0 14 3021.0 15 5916.0 16 8480.0 17 9030.0 18 9406.0 19 11255.0 20 13165.0 21 15085.0 22 15741.0 23 16979.0 24 17410.0 25 17254.0 26 14756.0 27 12310.0 28 13060.0 29 14940.0 30 14560.0 31 16854.0 32 21322.0 33 25996.0 34 31064.0 35 34690.0 36 33284.0 37 32230.0 38 20832.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.147892659196934 17.63766497866741 12.037960046253836 26.176482315881817 2 12.72822502107322 20.758963279402256 41.39544272857965 25.11736897094487 3 21.362673371844117 24.38916664223089 27.05315514764097 27.19500483828402 4 11.648857866749452 16.425288162071762 36.33616070981503 35.589693261363756 5 12.036996203358845 38.05521366630451 35.31537779987251 14.592412330464136 6 32.185504534916824 35.691829015333305 18.39345995851466 13.729206491235205 7 27.395891044716446 30.493314522992215 21.141643010949217 20.969151421342122 8 25.890060452692254 38.27170907727635 19.335301560522986 16.502928909508412 9 26.668765144059897 15.953904983941458 19.140016292025035 38.23731357997361 10 15.18876592043988 30.07051983392246 33.383654547494814 21.357059698142848 11 33.32025829913356 22.438958225265733 23.498219290318914 20.74256418528179 12 24.91692525380641 24.964396537345603 30.90985404871397 19.208824160134018 13 29.229679029485656 20.91101414782494 24.035187756181408 25.824119066508 14 20.361190858728193 23.688641890764785 26.58080262684261 29.369364623664413 15 24.24996304798064 31.171298876836694 22.351430621915842 22.227307453266825 16 20.63968354373992 28.084423972987178 30.0474862574949 21.228406225778 17 21.989987395227278 28.518476317982095 29.059698197588713 20.43183808920191 18 21.78167271961682 26.9458041651315 31.42025018860642 19.852272926645263 19 23.24351906405185 27.098939483208483 26.932006284543952 22.72553516819572 20 23.34535222395681 27.85460203379728 27.88769585564856 20.912349886597347 21 21.096468317159182 26.636650043651233 26.84686280499377 25.42001883419581 22 22.232369925952984 28.93506484382684 26.259291003358108 22.573274226862065 23 20.794908160088553 28.961742018056665 28.037289425438445 22.20606039641634 24 22.785255983093123 28.428878776916775 27.733754736020412 21.05211050396969 25 22.4486866160581 27.33934893792927 27.225540202330485 22.986424243682144 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 852.0 1 852.0 2 1588.0 3 2324.0 4 2324.0 5 2324.0 6 3079.5 7 3835.0 8 3835.0 9 3835.0 10 4316.0 11 4797.0 12 4797.0 13 4797.0 14 5690.0 15 6583.0 16 6583.0 17 6583.0 18 8842.0 19 11101.0 20 11101.0 21 11101.0 22 15399.5 23 19698.0 24 19698.0 25 19698.0 26 26494.0 27 33290.0 28 33290.0 29 33290.0 30 40961.0 31 48632.0 32 48632.0 33 48632.0 34 54017.0 35 59402.0 36 59402.0 37 59402.0 38 62481.0 39 65560.0 40 65560.0 41 65560.0 42 68454.5 43 71349.0 44 71349.0 45 71349.0 46 75950.0 47 80551.0 48 80551.0 49 80551.0 50 72588.5 51 64626.0 52 64626.0 53 64626.0 54 53923.5 55 43221.0 56 43221.0 57 43221.0 58 35799.0 59 28377.0 60 28377.0 61 28377.0 62 22902.5 63 17428.0 64 17428.0 65 17428.0 66 13457.0 67 9486.0 68 9486.0 69 9486.0 70 6984.5 71 4483.0 72 4483.0 73 4483.0 74 3230.0 75 1977.0 76 1977.0 77 1977.0 78 1398.5 79 820.0 80 820.0 81 820.0 82 561.5 83 303.0 84 303.0 85 303.0 86 204.0 87 105.0 88 105.0 89 105.0 90 67.5 91 30.0 92 30.0 93 30.0 94 18.5 95 7.0 96 7.0 97 7.0 98 3.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.346935320306061 2 36.66973603967956 3 5.73373851360573 4 13.743419995611891 5 13.816497563217279 6 15.046895758218634 7 9.258910539581954 8 7.836748514694119 9 0.11056653254715922 10 1.4686345205990632 11 5.74738656996702 12 5.743240324996501 13 16.378531434583486 14 1.6121982527032654 15 2.9884060625011877 16 3.044380369603187 17 2.9619737508141326 18 1.5318647563994698 19 1.4769270105401002 20 0.29196475000734234 21 4.4205881794011095 22 1.6358664010766417 23 0.11350345606794313 24 9.261156422274318 25 0.11644037958872704 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 578837.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.39516328018802 #Duplication Level Percentage of deduplicated Percentage of total 1 96.80975483512539 85.5751408576588 2 2.1817068772156576 3.8570467128197437 3 0.42682918748369475 1.1318890716111356 4 0.1694162136936418 0.5990229548704276 5 0.09469502908965284 0.4185291279101009 6 0.06010983996026809 0.3188051470820321 7 0.04141350798081135 0.25625276599784325 8 0.03109757094159943 0.21990998887999294 9 0.021513245389374276 0.17115001549924422 >10 0.1360150142808291 2.330509772061856 >50 0.011144588535382024 0.7023592816110363 >100 0.013621138734746978 2.2415263134505743 >500 0.002063808899187842 1.1651850381659157 >1k 6.191426697563527E-4 1.0126729523812428 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 2256 0.3897470272287362 No Hit GTACATGGGAAGCAGTGGTATCAAC 2211 0.3819728179090141 No Hit GAGTACATGGGAAGCAGTGGTATCA 1084 0.18727206450175093 No Hit GTCCTACAGTGGACATTTCTAAATT 921 0.15911215074364632 No Hit CATGTACTCTGCGTTGATACCACTG 848 0.14650065562498596 No Hit GCGTTGATACCACTGCTTCCCATGT 713 0.12317802766581958 No Hit ACGCAGAGTACATGGGAAGCAGTGG 644 0.11125757337557896 No Hit CTGTAGGACGTGGAATATGGCAAGA 625 0.10797512944058518 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTAC 500 0.0 17.108212 1 CTAGGAC 85 2.584784E-9 16.756075 3 CTCTAGG 65 4.4170636E-5 16.450205 1 GATATAC 175 0.0 16.03895 1 TGTAGGA 520 0.0 15.81614 2 CTGTAGG 510 0.0 15.72446 1 TCTAGGA 80 1.2117502E-5 15.420736 2 TCCTACA 600 0.0 15.306508 2 TAAGACC 50 5.9860706E-4 15.012828 4 TAGGACC 275 0.0 14.817857 4 GTATTAA 55 0.0045228996 14.580862 1 GTCTTAG 55 0.0045228996 14.580862 1 CCAACGA 55 3.218353E-5 13.909011 19 GCCATAT 125 3.0426236E-8 13.900421 1 TAAAACA 85 1.0068816E-6 13.877404 4 TGCAATA 40 0.0065248674 13.798896 11 GTAGGAC 580 0.0 13.411527 3 GTGTAGG 60 0.008050503 13.36579 1 GTGTAGC 80 2.5890395E-4 13.36579 1 TAGACAG 55 0.0015053186 13.289468 5 >>END_MODULE