FastQCFastQC Report
Thu 26 May 2016
SRR1926525_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926525_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578837
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC66111.1421177291707336No Hit
GTACATGGGAAGCAGTGGTATCAAC59661.03068739558805No Hit
GAGTACATGGGAAGCAGTGGTATCA26240.4533227834433528No Hit
CATGTACTCTGCGTTGATACCACTG22780.3935477517850448No Hit
GTCCTACAGTGGACATTTCTAAATT19090.32979923536332334No Hit
ACGCAGAGTACATGGGAAGCAGTGG17890.309068010510731No Hit
CTGTAGGACGTGGAATATGGCAAGA17840.3082042094752063No Hit
GCGTTGATACCACTGCTTCCCATGT17460.3016393216052187No Hit
GTATCAACGCAGAGTACATGGGAAG16840.2909281887647127No Hit
GTCCTACAGTGTGCATTTCTCATTT13430.2320169581419294No Hit
ACTCTGCGTTGATACCACTGCTTCC12340.21318609556749135No Hit
CTTTAGGACGTGAAATATGGCGAGG12280.21214953432486175No Hit
GCTTCCCATGTACTCTGCGTTGATA12020.20765776894013344No Hit
GTATCAACGCAGAGTACTTTTTTTT11710.20230220251988038No Hit
CTGTAGGACCTGGAATATGGCGAGA11650.20126564127725077No Hit
GTCCTAAAGTGTGTATTTCTCATTT11320.1955645544427879No Hit
TATCAACGCAGAGTACATGGGAAGC10270.17742473269676956No Hit
GGTATCAACGCAGAGTACATGGGAA9390.16222183447153515No Hit
CAGTGGTATCAACGCAGAGTACATG8620.14891929852445507No Hit
GATATACACTGTTCTACAAATCCCG8500.14684617603919584No Hit
GGTATCAACGCAGAGTACTTTTTTT7600.13129775739975155No Hit
CTGAAGGACCTGGAATATGGCGAGA7070.12214146642318995No Hit
GTGGTATCAACGCAGAGTACATGGG6790.11730418062425173No Hit
CATGGGAAGCAGTGGTATCAACGCA6450.11143033358268391No Hit
GCAGTGGTATCAACGCAGAGTACAT6280.10849341006189997No Hit
ACATGGGAAGCAGTGGTATCAACGC5940.10261956302033215No Hit
ATACCACTGCTTCCCATGTACTCTG5910.10210128239901733No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG456.5661396E-414.8326195
TCCAACG1350.014.73780118
TAGGACC2850.014.7210944
GTCCTAG954.611138E-914.055621
TCCTAGA1051.2787496E-913.62890152
GCCGGTT655.5494165E-513.12619911
CCGGTTT655.5494165E-513.12619912
TAGCACT801.9119634E-613.1109754
CCAACGA1102.8430804E-912.91950119
GGCGAGG3250.012.82674519
TGTAGGA10150.012.6889772
GTAGGAC9350.012.6489863
GATATAC2900.012.4977311
GTGTTAC701.0490375E-412.2628111
GTCCTAA3050.012.1958031
AACGTGA550.00305512712.0958177
CTGTAGG10350.011.97977351
ACTGTTC2950.011.9180268
CGGTTTC802.9212997E-511.85004113
TAGAGTG1052.5810914E-711.8055545
Produced by FastQC (version 0.11.5)