FastQCFastQC Report
Thu 26 May 2016
SRR1926524_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926524_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences582592
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC23800.4085191695045589No Hit
CCCATGTACTCTGCGTTGATACCAC23300.3999368340107657No Hit
GAGTACATGGGAAGCAGTGGTATCA11020.18915467428320334No Hit
GTCCTACAGTGGACATTTCTAAATT9960.17096012303636166No Hit
CATGTACTCTGCGTTGATACCACTG8300.14246676919696802No Hit
CTGTAGGACGTGGAATATGGCAAGA7830.13439937383280237No Hit
GCGTTGATACCACTGCTTCCCATGT7770.1333694935735472No Hit
GTATCAACGCAGAGTACATGGGAAG7200.12358563111062287No Hit
ACGCAGAGTACATGGGAAGCAGTGG6410.11002554103042954No Hit
GTCCTAAAGTGTGTATTTCTCATTT5960.10230143908601559No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGTC350.001380469517.421027
GATATAC2100.015.8644051
TAGGACC2600.015.6340594
TGTAGGA5350.015.5696432
GTCCTAA3450.015.4505511
GTCCTAC6500.015.3762681
TCTATAC651.4737827E-515.1072553
CTACACT951.5452315E-814.6379834
CTGTAGG5650.014.6233311
TTTGGAC450.00459427614.5477293
GATTAAA550.004613312414.5375631
TCCTAAA3700.014.393472
GTGTAGA658.417712E-414.3511841
GATCTAC751.5360599E-414.2145071
TTTGGCG350.00561961614.1139316
TCCTACA7650.014.1015962
TACACAA851.4618672E-613.4257875
GTAGGAC5450.013.4132723
CCTACAG8650.012.9920453
TCTAGGA956.343527E-512.9366412
Produced by FastQC (version 0.11.5)