##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926524_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 582592 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.53250473744919 27.0 2.0 30.0 2.0 31.0 2 16.037908175876083 16.0 2.0 30.0 2.0 31.0 3 19.51180929363946 22.0 15.0 30.0 2.0 31.0 4 21.592943260463585 27.0 2.0 35.0 2.0 37.0 5 21.86443514500714 28.0 2.0 35.0 2.0 35.0 6 22.81320546797759 30.0 2.0 35.0 2.0 37.0 7 22.688335919477094 30.0 2.0 35.0 2.0 37.0 8 23.13347934746787 30.0 2.0 35.0 2.0 37.0 9 25.640879037130617 31.0 17.0 37.0 2.0 39.0 10 26.29827563715259 31.0 18.0 37.0 2.0 39.0 11 23.451844515544327 30.0 7.0 37.0 2.0 39.0 12 24.48635408656487 30.0 8.0 37.0 2.0 39.0 13 21.25839352411293 27.0 2.0 35.0 2.0 39.0 14 24.527374217291 30.0 10.0 37.0 2.0 40.0 15 25.586465313632868 31.0 11.0 38.0 2.0 40.0 16 25.93419751730199 31.0 10.0 38.0 2.0 40.0 17 25.246215190047238 31.0 8.0 38.0 2.0 40.0 18 25.313988176974625 31.0 10.0 38.0 2.0 40.0 19 25.690318438976163 31.0 8.0 38.0 2.0 40.0 20 25.944504902230033 31.0 2.0 39.0 2.0 40.0 21 23.7964630478963 30.0 2.0 37.0 2.0 40.0 22 24.740549132154236 31.0 2.0 38.0 2.0 40.0 23 24.72305146654949 31.0 2.0 38.0 2.0 40.0 24 23.817860526749424 31.0 2.0 38.0 2.0 40.0 25 23.610823698231354 30.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 4.985806047088243 1101 2 9.558190542569607 1101 3 6.212056687780375 1101 4 8.015605726232316 1101 5 7.5945509499183785 1101 6 7.258805078219478 1101 7 6.641400628700847 1101 8 5.222842261881578 1101 9 5.155037670688365 1101 10 4.653999461416376 1101 11 7.426650963656876 1101 12 5.0791991723793934 1101 13 9.53008461068664 1101 14 6.8579196061331835 1101 15 5.969945120690099 1101 16 5.4364731397679975 1101 17 1.0939981555205662 1101 18 3.7387879119022394 1101 19 4.5546680732680045 1101 20 2.8425035992183254 1101 21 -0.6102298057501905 1101 22 3.5676283864107567 1101 23 4.734915877575002 1101 24 5.970344281249311 1101 25 6.30824967270042 1102 1 5.269975035543478 1102 2 10.497656984230423 1102 3 6.7581470223444455 1102 4 8.185444560128161 1102 5 7.559964794573734 1102 6 6.494569494072575 1102 7 6.644946547260808 1102 8 7.036581964843652 1102 9 5.606777502009482 1102 10 5.131668763218688 1102 11 7.828551621308208 1102 12 5.096840990245461 1102 13 9.685156491750657 1102 14 7.312156775891793 1102 15 4.927110953022986 1102 16 5.395580402624667 1102 17 6.3206566298600855 1102 18 6.193558337978757 1102 19 4.91282094888809 1102 20 5.1762607061565085 1102 21 4.306437804732969 1102 22 5.054041204193769 1102 23 5.486403332366194 1102 24 6.971860994351751 1102 25 6.8606494205126545 1103 1 4.112366224816217 1103 2 8.420841405141685 1103 3 5.364232377020986 1103 4 7.3517043870473415 1103 5 6.254281439915346 1103 6 5.862077366194825 1103 7 6.302094071285527 1103 8 5.619714903134078 1103 9 4.747882835693694 1103 10 3.9029976016534 1103 11 4.798018978846237 1103 12 3.824687885407851 1103 13 7.863255506563256 1103 14 6.017317439781664 1103 15 5.002634929344612 1103 16 4.654756983174398 1103 17 2.1939283171409727 1103 18 1.4520089591277667 1103 19 3.191888817017567 1103 20 3.584540273121725 1103 21 5.725839674683392 1103 22 1.6403381155390022 1103 23 3.9051367147017864 1103 24 5.4297689608487225 1103 25 1.1977970655306045 1104 1 5.003939549061933 1104 2 9.480200907235721 1104 3 6.408364135565293 1104 4 7.070840106479007 1104 5 6.820326620935372 1104 6 6.618899807835259 1104 7 6.541657506764913 1104 8 6.541546417448576 1104 9 5.2660446252375515 1104 10 4.175784661359668 1104 11 7.184544787390394 1104 12 4.197713794095197 1104 13 8.372266898972104 1104 14 6.149004239713509 1104 15 5.639686115966676 1104 16 5.221483735754955 1104 17 5.3260605300663215 1104 18 3.2562992727403675 1104 19 3.876939979724842 1104 20 3.269116129345999 1104 21 2.483394506816502 1104 22 4.431887823928012 1104 23 4.74259983360912 1104 24 5.758681047085464 1104 25 4.754787508582012 1105 1 3.0136382187886497 1105 2 9.215806613668919 1105 3 6.105339499300825 1105 4 5.150197495233311 1105 5 1.854361216540795 1105 6 4.3132939597329205 1105 7 5.184724927111439 1105 8 2.34301410979986 1105 9 3.5032611098593627 1105 10 3.215012847852929 1105 11 6.386389319669711 1105 12 3.840208871092379 1105 13 4.9613187073787515 1105 14 3.6447074145190683 1105 15 3.9299406954218163 1105 16 4.218573851498647 1105 17 5.112981634518981 1105 18 2.471208533652433 1105 19 2.482470743179867 1105 20 1.5665816683262648 1105 21 2.5808855462430245 1105 22 4.144934135571798 1105 23 1.5174932275768462 1105 24 4.46252169763018 1105 25 5.210800655478749 1106 1 6.378415429037396 1106 2 11.207711711740705 1106 3 7.791113899588744 1106 4 9.28313217402572 1106 5 8.490289957041604 1106 6 7.620233040335883 1106 7 7.716585453390799 1106 8 7.447982065172699 1106 9 6.505813606295003 1106 10 5.819721954617599 1106 11 8.176730252355227 1106 12 6.006658215665553 1106 13 4.965712212631853 1106 14 6.172982818364421 1106 15 6.0569038614032955 1106 16 6.262531051504183 1106 17 6.727136639156111 1106 18 6.894181001573202 1106 19 6.736012262773464 1106 20 6.186741406986066 1106 21 6.327526743314021 1106 22 6.596172317817828 1106 23 7.116075789209116 1106 24 8.266751865258879 1106 25 7.041270043516345 1107 1 3.9517421398011194 1107 2 -13.490473579567796 1107 3 -5.351617607731244 1107 4 2.0738491829029897 1107 5 4.406323073450178 1107 6 4.967680280086256 1107 7 4.828760566949175 1107 8 5.3038913288479925 1107 9 3.613043155773344 1107 10 3.2049908318339106 1107 11 5.894194876350827 1107 12 3.0295808517687064 1107 13 4.377659185788193 1107 14 3.8888799228594912 1107 15 3.9045722985391755 1107 16 3.6954984522613294 1107 17 4.270699172146564 1107 18 -0.615745286734672 1107 19 1.9780751932071077 1107 20 2.2708124358253983 1107 21 -0.8131912892131226 1107 22 2.1015171053481723 1107 23 -0.6834072230987154 1107 24 2.474101493317921 1107 25 3.0623892755831115 1108 1 5.24326606414575 1108 2 10.600939162814475 1108 3 0.5060995993985138 1108 4 5.739052929012264 1108 5 6.970953612863408 1108 6 7.057948502267745 1108 7 7.357792225410581 1108 8 7.193988991703321 1108 9 5.756899018513533 1108 10 4.787055081583709 1108 11 7.138880387155357 1108 12 2.7073278508453456 1108 13 3.7502383749687773 1108 14 4.857465855243301 1108 15 5.044180528413907 1108 16 4.75328553536124 1108 17 0.22385989806495488 1108 18 3.3053809211428984 1108 19 4.556184394202926 1108 20 4.7727026152396945 1108 21 5.048968033162076 1108 22 1.6572014552822125 1108 23 4.447191997767007 1108 24 5.108981477897018 1108 25 5.501664734058114 1109 1 2.831445228839616 1109 2 -13.490473579567796 1109 3 -5.613889262218873 1109 4 1.4389905250445345 1109 5 0.6850835763824854 1109 6 1.875162927017449 1109 7 3.267682891482231 1109 8 3.191055642406184 1109 9 2.6843816170582855 1109 10 1.914651240837184 1109 11 0.5396081915697124 1109 12 1.1307596336940513 1109 13 2.3690462777782244 1109 14 2.9651253980616765 1109 15 2.749290941713209 1109 16 2.680558608956197 1109 17 3.1518368303657205 1109 18 3.269138547034739 1109 19 1.8788537239226315 1109 20 0.7221789842720909 1109 21 0.13233719435478974 1109 22 2.062289374128028 1109 23 2.468936333790605 1109 24 3.35066079769377 1109 25 3.722954827246827 1110 1 3.62062953493119 1110 2 9.090401293270455 1110 3 6.505788681288333 1110 4 7.58682892519483 1110 5 8.160693503093345 1110 6 4.110259769808554 1110 7 6.747914610669117 1110 8 7.125069436832849 1110 9 5.4806290213597 1110 10 5.03776779794655 1110 11 3.133310162401955 1110 12 5.691839244444189 1110 13 9.963082016975225 1110 14 7.43332846513313 1110 15 6.956697001907433 1110 16 6.214063356661292 1110 17 6.77786336794864 1110 18 6.79298599690453 1110 19 5.709682129009501 1110 20 6.025722325193492 1110 21 4.334858644468412 1110 22 5.915092528738729 1110 23 6.409216779161515 1110 24 7.293862215909527 1110 25 7.519542995058366 1111 1 5.161270879396522 1111 2 9.889310522493046 1111 3 7.565259947974102 1111 4 8.177436476331572 1111 5 8.736183406525292 1111 6 7.821157594157121 1111 7 7.755077598338794 1111 8 8.032380875550121 1111 9 6.4402287725769085 1111 10 5.848142799069667 1111 11 7.490102821113297 1111 12 6.7763914503948115 1111 13 8.672342586999292 1111 14 6.566716772940936 1111 15 6.253878628319942 1111 16 5.76483071979332 1111 17 6.996828163985686 1111 18 7.087365322775042 1111 19 5.766791356834439 1111 20 5.348973233420395 1111 21 8.036582852970753 1111 22 7.095282350407789 1111 23 7.214953558582426 1111 24 7.012369279563778 1111 25 7.8771383077190364 1112 1 -1.7547539085355588 1112 2 7.9262732924245025 1112 3 6.090262757915344 1112 4 0.2455400068456477 1112 5 4.297677671883864 1112 6 3.190215749078863 1112 7 5.930588889579994 1112 8 6.021198726185922 1112 9 4.713614803721448 1112 10 3.2570822847417986 1112 11 0.12828439715502782 1112 12 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2204 7 4.938453380653797 2204 8 5.173647204406901 2204 9 3.5307653862555384 2204 10 3.2213428540560543 2204 11 4.252196007765349 2204 12 4.759152836204503 2204 13 3.1702032728781013 2204 14 4.100549629598298 2204 15 4.256828418875266 2204 16 4.152088579219093 2204 17 -1.472227673926664 2204 18 2.173545983604498 2204 19 3.0287413497702182 2204 20 3.456647271063158 2204 21 5.098546592503293 2204 22 4.414622475578188 2204 23 3.3599047390714247 2204 24 4.9282964302379355 2204 25 5.005182128814717 2205 1 5.901940791149194 2205 2 10.33573724664303 2205 3 6.939155924421598 2205 4 7.7321290365851425 2205 5 7.9077573105165975 2205 6 7.1808607461402865 2205 7 7.466099054862376 2205 8 7.435941150552988 2205 9 6.287694818930756 2205 10 5.375217645738886 2205 11 7.680530283375891 2205 12 7.043825866013719 2205 13 10.135487748214459 2205 14 7.689490436113683 2205 15 6.64209231192126 2205 16 6.165425496901015 2205 17 6.7393163304188946 2205 18 6.5721375563205875 2205 19 5.112752753172021 2205 20 5.4445122187205826 2205 21 -0.430890332199521 2205 22 4.0240125450849895 2205 23 5.26542666826391 2205 24 7.019068210698432 2205 25 7.540574799745649 2206 1 4.18187838442012 2206 2 8.497145468051253 2206 3 6.051536876802551 2206 4 1.7801767912995636 2206 5 5.8586676347602555 2206 6 5.988490647103518 2206 7 6.1962225116525005 2206 8 6.098611073222909 2206 9 4.805014042916646 2206 10 3.8901681273560342 2206 11 3.302207125052732 2206 12 4.830533232721088 2206 13 8.093699659759704 2206 14 0.3353078285977418 2206 15 2.5631912130201613 2206 16 3.4376490757912634 2206 17 4.689434360536925 2206 18 -0.9920626878796597 2206 19 1.057777851530453 2206 20 2.638852199727232 2206 21 3.398663324020802 2206 22 -1.5692963682239238 2206 23 -2.4814163652457886 2206 24 1.5354133470435691 2206 25 3.1692547617589426 2207 1 1.8140744084608045 2207 2 -13.490473579567796 2207 3 -5.886086794025003 2207 4 -3.9175948222002077 2207 5 1.3538687443302955 2207 6 2.234990878263943 2207 7 3.4170639356007193 2207 8 3.463604138410151 2207 9 1.1528272652929736 2207 10 0.8697335457564037 2207 11 -0.6947138180818015 2207 12 -2.371390022017703 2207 13 0.7551698400648341 2207 14 -4.5511276776435885 2207 15 -0.8855651438915366 2207 16 0.11500460052542749 2207 17 1.3591708376520444 2207 18 1.3111550652410067 2207 19 0.916427874646498 2207 20 0.8051305168793341 2207 21 -2.998618229377257 2207 22 -3.4499066317468063 2207 23 -0.44436597227306507 2207 24 1.2694679009039476 2207 25 1.8339796808527957 2208 1 4.502534311282943 2208 2 6.332452795082718 2208 3 5.986686213331197 2208 4 7.770960086910446 2208 5 3.297282998892861 2208 6 6.048761361167337 2208 7 6.481137081394657 2208 8 6.432502698838672 2208 9 4.736062727068777 2208 10 4.203126898935498 2208 11 6.621128706732936 2208 12 6.0631316439401886 2208 13 8.755105597717364 2208 14 2.656820277006741 2208 15 4.225168757066669 2208 16 4.743960707620136 2208 17 5.467344092929416 2208 18 0.3376563908049377 2208 19 2.8354843264783085 2208 20 3.940896312805826 2208 21 6.383453631569729 2208 22 5.507854477202784 2208 23 1.6521006986161026 2208 24 3.0267575976981647 2208 25 4.325203814259719 2209 1 2.932711717753456 2209 2 4.5948438807496625 2209 3 5.210981321246994 2209 4 -0.020100986478215788 2209 5 4.924461285553907 2209 6 5.338768424881298 2209 7 6.0697145751445625 2209 8 4.345714247826084 2209 9 4.243823566726167 2209 10 3.3826284448163513 2209 11 -0.6156897003440918 2209 12 3.052398312086165 2209 13 2.320842516902559 2209 14 3.769474495264408 2209 15 4.214818197147146 2209 16 3.884196694838881 2209 17 1.6771724557750218 2209 18 3.6860722327552615 2209 19 4.022619121371722 2209 20 4.1467490251240555 2209 21 6.388028403385881 2209 22 5.264473473045918 2209 23 0.3539407776113528 2209 24 -2.7929993513691294 2209 25 0.23770714271132576 2210 1 -1.2981924363610204 2210 2 -13.490473579567796 2210 3 -7.033097127742664 2210 4 -1.4641633598552382 2210 5 0.02662070277991546 2210 6 -0.5413988936184282 2210 7 -0.6842140403267827 2210 8 -20.498531783919947 2210 9 -12.547988441095828 2210 10 -6.581091319819063 2210 11 -9.211580729711816 2210 12 -4.007045119374467 2210 13 -4.750704970016736 2210 14 -3.5826309695630307 2210 15 -5.051439091932011 2210 16 -4.4461604480182615 2210 17 -4.205989771379993 2210 18 -4.105390736080071 2210 19 -9.041888128068933 2210 20 -6.0783657063031065 2210 21 -4.167196831362105 2210 22 -9.41109754152343 2210 23 -7.226304056171216 2210 24 -9.959961104623892 2210 25 -7.458196218633214 2211 1 -0.17336574491059764 2211 2 4.462007941023545 2211 3 0.3911094619083997 2211 4 1.5489946119864442 2211 5 0.8181124982389107 2211 6 -0.26186787856651605 2211 7 -20.077110821680833 2211 8 -13.359144244321215 2211 9 -9.382974090034528 2211 10 -7.426616706957837 2211 11 -9.887356911755234 2211 12 -5.147821471356952 2211 13 -6.88352465763357 2211 14 -5.198057686562915 2211 15 -5.53520170697783 2211 16 -11.887554323414248 2211 17 -7.9962919933626075 2211 18 -6.429793869053288 2211 19 -8.804778592377627 2211 20 -7.122527596119301 2211 21 -4.894346179675079 2211 22 -6.558628092465888 2211 23 -8.723883303446623 2211 24 -21.368396176765955 2211 25 -21.16407857157439 2212 1 -0.45793113938940166 2212 2 -13.490473579567796 2212 3 -7.360272647006973 2212 4 -19.084585113462342 2212 5 -19.244189508096888 2212 6 -20.156271257658386 2212 7 -9.371967964537976 2212 8 -8.714531783919947 2212 9 -4.350795480892877 2212 10 -4.7351652059772995 2212 11 -5.102935732090124 2212 12 -2.184133433945579 2212 13 -5.164014625954584 2212 14 -2.9469778856879714 2212 15 -2.994713548884601 2212 16 -3.1341604480182603 2212 17 -3.367708496605932 2212 18 -2.3680626878796573 2212 19 -3.098984053231451 2212 20 -3.150481133606103 2212 21 -1.2104795331220544 2212 22 -2.259582082509638 2212 23 -2.55779731762674 2212 24 -1.5466818910516693 2212 25 -1.9103642858601049 2213 1 -18.076216853675117 2213 2 -13.490473579567796 2213 3 -8.307016042598425 2213 4 -5.163501485664934 2213 5 -19.244189508096888 2213 6 -10.251677618082415 2213 7 -20.077110821680833 2213 8 -10.431393974732668 2213 9 -8.094410490147464 2213 10 -6.939900861370198 2213 11 -6.4682017593490855 2213 12 -4.919771327265451 2213 13 -18.572014625954584 2213 14 -11.95908221010325 2213 15 -6.823795157499781 2213 16 -5.989152203024151 2213 17 -4.462771596050658 2213 18 -4.749296408222918 2213 19 -5.60834027063007 2213 20 -5.407225538788669 2213 21 -3.5190751918802583 2213 22 -4.402673450505599 2213 23 -4.288863782375184 2213 24 -3.2623891096281454 2213 25 -4.281864201727512 2214 1 -18.076216853675117 2214 2 -13.490473579567796 2214 3 -11.649849311180496 2214 4 -19.084585113462342 2214 5 -19.244189508096888 2214 6 -20.156271257658386 2214 7 -15.956691973513294 2214 8 -14.357170527375445 2214 9 -12.890089422553313 2214 10 -12.738340972619033 2214 11 -13.775474251162676 2214 12 -11.31596888645119 2214 13 -18.572014625954584 2214 14 -15.78158820730353 2214 15 -23.07585640602746 2214 16 -17.23804526477219 2214 17 -22.801994210891646 2214 18 -16.837358125426405 2214 19 -13.71714860072584 2214 20 -12.461915688579925 2214 21 -9.988457094827366 2214 22 -11.053469891036189 2214 23 -10.901029927948356 2214 24 -9.922061098231923 2214 25 -9.647062864768106 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 113801.0 3 12.0 4 563.0 5 1977.0 6 2744.0 7 2530.0 8 2360.0 9 2258.0 10 2070.0 11 1862.0 12 1864.0 13 1959.0 14 2959.0 15 5985.0 16 8799.0 17 9337.0 18 9744.0 19 10803.0 20 13151.0 21 15183.0 22 15827.0 23 17250.0 24 18308.0 25 18563.0 26 15482.0 27 12566.0 28 13711.0 29 15585.0 30 15271.0 31 17327.0 32 22001.0 33 27529.0 34 32871.0 35 36826.0 36 35278.0 37 35383.0 38 22853.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.67369094622731 18.320116171031334 12.931159154709146 25.075033728032203 2 12.322792005905587 20.215855636440054 42.96798805950848 24.493364298145885 3 20.86686144105098 24.929237846497614 27.873089239596187 26.33081147285522 4 11.367294575125902 17.186404347548926 37.273299547395936 34.173001529929245 5 11.810163929878515 38.06311884866654 35.97580186538846 14.150915356066477 6 31.285107411061226 36.37157985866436 18.97823592180233 13.365076808472084 7 26.733920862969573 30.940519708691095 22.520143198541568 19.805416229797764 8 23.85308688424022 40.29954203170379 20.077026152936575 15.770344931119418 9 26.232815160632395 16.5063807925001 19.765765549047135 37.495038497820374 10 14.85404527020194 30.934173039436196 33.717443080968174 20.49433860939369 11 32.502779853807034 23.295357188428518 23.974370659326638 20.227492298437813 12 24.480455874546944 25.59314582987518 31.504995248266667 18.421403047311212 13 28.988839431825618 21.847998625384566 24.42581167768541 24.737350265104403 14 19.736920213564638 24.762812656044307 26.988899248193814 28.51136788219724 15 23.96095155381897 32.13890888636931 22.627085216388462 21.27305434342327 16 19.821097704766885 28.584827879679786 30.980061879981207 20.614012535572122 17 20.90336933205773 29.25870450570574 30.033440439411972 19.80448572282456 18 20.83699702717414 27.548011835475723 32.645256737707435 18.9697343996427 19 22.716073492973806 27.55323916254139 27.38200897301991 22.34867837146489 20 22.85473804515834 28.914347875478935 28.338594576730962 19.892319502631768 21 20.72085666399004 27.184997961093714 27.195955959624268 24.898189415291977 22 21.244523909714573 30.110682937646345 26.989944422812663 21.654848729826423 23 20.345550457927384 29.445675893946422 28.655085313675965 21.55368833445023 24 22.193724451087636 29.1066043112787 28.428130885922105 20.271540351711565 25 21.68232682182478 27.800269441733228 28.224193393178727 22.29321034326327 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1010.0 1 1010.0 2 1827.0 3 2644.0 4 2644.0 5 2644.0 6 3506.5 7 4369.0 8 4369.0 9 4369.0 10 5004.0 11 5639.0 12 5639.0 13 5639.0 14 6531.5 15 7424.0 16 7424.0 17 7424.0 18 10236.0 19 13048.0 20 13048.0 21 13048.0 22 18029.0 23 23010.0 24 23010.0 25 23010.0 26 30422.5 27 37835.0 28 37835.0 29 37835.0 30 46265.0 31 54695.0 32 54695.0 33 54695.0 34 59415.5 35 64136.0 36 64136.0 37 64136.0 38 66457.0 39 68778.0 40 68778.0 41 68778.0 42 70258.0 43 71738.0 44 71738.0 45 71738.0 46 75134.0 47 78530.0 48 78530.0 49 78530.0 50 69651.5 51 60773.0 52 60773.0 53 60773.0 54 49447.5 55 38122.0 56 38122.0 57 38122.0 58 31024.0 59 23926.0 60 23926.0 61 23926.0 62 19022.5 63 14119.0 64 14119.0 65 14119.0 66 10792.5 67 7466.0 68 7466.0 69 7466.0 70 5384.0 71 3302.0 72 3302.0 73 3302.0 74 2332.5 75 1363.0 76 1363.0 77 1363.0 78 904.0 79 445.0 80 445.0 81 445.0 82 299.0 83 153.0 84 153.0 85 153.0 86 99.5 87 46.0 88 46.0 89 46.0 90 33.0 91 20.0 92 20.0 93 20.0 94 10.0 95 0.0 96 0.0 97 0.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.357375315829948 2 36.28920410853565 3 5.822771339118972 4 13.836097989673735 5 13.37556986707679 6 14.744795671756563 7 9.141388827858947 8 7.3868161595078545 9 0.10487613973415358 10 1.5547758980555861 11 5.8363314291991655 12 5.899669065143359 13 16.088102823245084 14 1.688660331758761 15 2.959704218389542 16 3.192113863561463 17 2.936703559266176 18 1.6134790728331319 19 1.561126826320993 20 0.27703778973964627 21 4.4492543666922995 22 1.695011260024168 23 0.1067642535427881 24 9.050244424914863 25 0.11122706799956059 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 582592.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.62214043617344 #Duplication Level Percentage of deduplicated Percentage of total 1 96.51869232575304 83.60655721357196 2 2.2925709737300806 3.971748096926839 3 0.4816967515624254 1.251768109844669 4 0.21181520507512586 0.7339154576213771 5 0.11442090167513876 0.4955691706868732 6 0.07475800815948071 0.38854192089114836 7 0.0501861105434421 0.30430598208075565 8 0.03355225150690837 0.2325094273584983 9 0.026200590183086406 0.20425960821149441 >10 0.16177822846326845 2.6782359752364147 >50 0.015795406982194265 0.962870084754513 >100 0.015584775353080185 2.7393782128441693 >500 0.0023166557957156656 1.3700539676613352 >1k 6.318152170133633E-4 1.060286772309944 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 2380 0.4085191695045589 No Hit CCCATGTACTCTGCGTTGATACCAC 2330 0.3999368340107657 No Hit GAGTACATGGGAAGCAGTGGTATCA 1102 0.18915467428320334 No Hit GTCCTACAGTGGACATTTCTAAATT 996 0.17096012303636166 No Hit CATGTACTCTGCGTTGATACCACTG 830 0.14246676919696802 No Hit CTGTAGGACGTGGAATATGGCAAGA 783 0.13439937383280237 No Hit GCGTTGATACCACTGCTTCCCATGT 777 0.1333694935735472 No Hit GTATCAACGCAGAGTACATGGGAAG 720 0.12358563111062287 No Hit ACGCAGAGTACATGGGAAGCAGTGG 641 0.11002554103042954 No Hit GTCCTAAAGTGTGTATTTCTCATTT 596 0.10230143908601559 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 1.716467098758651E-4 0.0 6 0.0 0.0 0.0 1.716467098758651E-4 0.0 7 0.0 0.0 0.0 1.716467098758651E-4 0.0 8 0.0 0.0 0.0 1.716467098758651E-4 0.0 9 0.0 0.0 0.0 1.716467098758651E-4 0.0 10 0.0 0.0 0.0 1.716467098758651E-4 0.0 11 0.0 0.0 0.0 1.716467098758651E-4 0.0 12 0.0 0.0 0.0 1.716467098758651E-4 0.0 13 0.0 0.0 0.0 1.716467098758651E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACCGTC 35 0.0013804695 17.42102 7 GATATAC 210 0.0 15.864405 1 TAGGACC 260 0.0 15.634059 4 TGTAGGA 535 0.0 15.569643 2 GTCCTAA 345 0.0 15.450551 1 GTCCTAC 650 0.0 15.376268 1 TCTATAC 65 1.4737827E-5 15.107255 3 CTACACT 95 1.5452315E-8 14.637983 4 CTGTAGG 565 0.0 14.623331 1 TTTGGAC 45 0.004594276 14.547729 3 GATTAAA 55 0.0046133124 14.537563 1 TCCTAAA 370 0.0 14.39347 2 GTGTAGA 65 8.417712E-4 14.351184 1 GATCTAC 75 1.5360599E-4 14.214507 1 TTTGGCG 35 0.005619616 14.11393 16 TCCTACA 765 0.0 14.101596 2 TACACAA 85 1.4618672E-6 13.425787 5 GTAGGAC 545 0.0 13.413272 3 CCTACAG 865 0.0 12.992045 3 TCTAGGA 95 6.343527E-5 12.936641 2 >>END_MODULE