FastQCFastQC Report
Thu 26 May 2016
SRR1926524_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926524_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences582592
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC65501.1242859496869164No Hit
GTACATGGGAAGCAGTGGTATCAAC60471.0379476546193562No Hit
GAGTACATGGGAAGCAGTGGTATCA29760.5108206085905745No Hit
CATGTACTCTGCGTTGATACCACTG23690.40663105569592445No Hit
CTGTAGGACGTGGAATATGGCAAGA21060.36148797099857194No Hit
GCGTTGATACCACTGCTTCCCATGT20670.3547937493134132No Hit
GTCCTACAGTGGACATTTCTAAATT20070.3444949467208613No Hit
ACGCAGAGTACATGGGAAGCAGTGG19420.33333791057893003No Hit
GTATCAACGCAGAGTACATGGGAAG17290.29677716137537075No Hit
GTATCAACGCAGAGTACTTTTTTTT15290.26244781940019773No Hit
CTTTAGGACGTGAAATATGGCGAGG13810.2370441063385697No Hit
GTCCTAAAGTGTGTATTTCTCATTT13760.2361858727891904No Hit
ACTCTGCGTTGATACCACTGCTTCC13520.2320663517521696No Hit
GTCCTACAGTGTGCATTTCTCATTT12740.21867790838185214No Hit
GATATACACTGTTCTACAAATCCCG12430.21335686037570034No Hit
CTGTAGGACCTGGAATATGGCGAGA12300.21112545314731407No Hit
TATCAACGCAGAGTACATGGGAAGC11940.2049461715917829No Hit
GCTTCCCATGTACTCTGCGTTGATA11740.2015132373942656No Hit
GGTATCAACGCAGAGTACATGGGAA11730.20134159068438975No Hit
GGTATCAACGCAGAGTACTTTTTTT10620.18228880588816873No Hit
GTGTATATCAATGAGTTACAATGAA8870.15225063165989233No Hit
CAGTGGTATCAACGCAGAGTACATG8720.14967593101175436No Hit
GTGTATATCAATGAGTTACAATGAG8220.1410935955179611No Hit
CTGAAGGACCTGGAATATGGCGAGA7970.1368024277710645No Hit
TATCAACGCAGAGTACTTTTTTTTT7710.132339613314292No Hit
GTGGTATCAACGCAGAGTACATGGG7390.1268469185982643No Hit
GATATACACTGTTCTACAATGCCGG7340.125988685048885No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7070.12135422388223664No Hit
CATGGGAAGCAGTGGTATCAACGCA7050.1210109304624849No Hit
GTTCTACAGTGTGGTTTTTATCATT6950.11929446336372625No Hit
GCAGTGGTATCAACGCAGAGTACAT6890.11826458310447105No Hit
ATACCACTGCTTCCCATGTACTCTG6630.11380176864769856No Hit
ACATGGGAAGCAGTGGTATCAACGC6590.1131151818081951No Hit
TTGTAGAACAGTGTATATCAATGAG6360.1091673074810502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5100.015.1546414
TCCAACG1900.014.95608318
TGTAGGA12400.013.7752572
CTGTAGG12050.013.7722621
CCAACGT707.479879E-613.52937119
GTAGGAC12350.013.4446853
GTATAGG500.001453540413.3527611
CCAACGA1351.8189894E-1213.32893819
TAGGACT801.8973133E-613.1199894
GACGTGG5800.012.9439967
ATTATAC603.9336705E-412.72351553
CGTGGAA6150.012.3597419
GGCGAGG3400.012.25601919
AGGACCT7750.012.1888935
AGGACGT9650.012.1621015
GGACTGC550.002969126412.1420236
GGCCTAC550.00297490212.1388741
ACGTGGA6350.012.1200888
GGACGTG9400.012.0774386
GGACCTG7900.012.0761536
Produced by FastQC (version 0.11.5)