FastQCFastQC Report
Thu 26 May 2016
SRR1926523_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926523_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660138
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC23410.354622821288882No Hit
CCCATGTACTCTGCGTTGATACCAC22160.33568738657674607No Hit
GAGTACATGGGAAGCAGTGGTATCA11100.16814666024376723No Hit
GTCCTACAGTGGACATTTCTAAATT10900.16511699068982547No Hit
CTGTAGGACGTGGAATATGGCAAGA8620.13057875777488948No Hit
CATGTACTCTGCGTTGATACCACTG8370.12679167083246232No Hit
GCGTTGATACCACTGCTTCCCATGT8080.12239864997924677No Hit
GTCCTACAGTGTGCATTTCTCATTT7220.10937107089729722No Hit
GTCCTAAAGTGTGTATTTCTCATTT6970.10558398395487004No Hit
ACGCAGAGTACATGGGAAGCAGTGG6730.10194838049013995No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGTT304.7562018E-420.41387
CCCTAAA405.3433236E-420.0625511
CTTGGGC300.007448274618.3148543
CGTAGCG300.007448274618.3148543
TACACCG350.001144101417.9163935
TGTAGGA6650.017.7423922
ACACCGT300.00996937417.411376
GCAGGAC452.2285977E-417.0938643
CTGTAGG7000.016.6232571
TTCTAGA756.5181466E-616.463292
TTGTAGA654.3935193E-516.461581
GTATAAA654.3935193E-516.461581
TGTAGAA2350.016.3465292
TAGGACC2500.016.339014
CGTGTAA350.00264789215.8047579
TAGACTG601.0387494E-515.6768435
GTAGGAC7350.015.100413
CCTACAC951.0842996E-815.0374583
GATATAC1800.014.8611491
GTAGGAT450.0043826514.6518833
Produced by FastQC (version 0.11.5)